The kinetics of displacement of a fluorescent nucleotide, 2′(3′)- O-[N[2-[[Cy3]amido]ethyl]carbamoyl]-adenosine 5′-triphosphate (Cy3-EDA-ATP), bound to rabbit soleus muscle myofibrils were studied using flash photolysis of caged ATP. Use of myofibrils from this slow twitch muscle allowed better resolution of the kinetics of nucleotide exchange than previous studies with psoas muscle myofibrils (Chaen et al., 1997, Biophys. J. 73:2033–2042). Soleus myofibrils in the presence of Cy3-EDA-nucleotides (Cy3-EDA-ATP or Cy3-EDA-ADP) showed selective fluorescence staining of the A-band. The K m for Cy3-EDA-ATP and the K d for Cy3-EDA-ADP binding to the myofibril A-band were 1.9 μM and 3.8 μM, respectively, indicating stronger binding of nucleotide to soleus cross-bridges compared to psoas cross-bridges (2.6 μM and 50 μM, respectively). After flash photolysis of caged ATP, the A-band fluorescence of the myofibril in the Cy3-EDA-ATP solution under isometric conditions decayed exponentially with a rate constant of 0.045 ± 0.007 s −1 ( n = 32) at 10°C, which was about seven times slower than that for psoas myofibrils. When a myofibril was allowed to shorten with a constant velocity, the nucleotide displacement rate constant increased from 0.066 s −1 (isometric) to 0.14 s −1 at 20°C with increasing shortening velocity up to 0.1 myofibril length/s ( V max, the shortening velocity under no load was ∼0.2 myofibril lengths/s). The rate constant was not significantly affected by an isovelocity stretch of up to 0.1 myofibril lengths/s. These results suggest that the cross-bridge kinetics are not significantly affected at higher strain during lengthening but depend on the lower strain during shortening. These data also indicate that the interaction distance between a cross-bridge and the actin filament is at least 16 nm for a single cycle of the ATPase.