Cage Temperature Research Articles

Odorless Probiotic Chicken of Eco-Smart Poultry Coop

The smell of chicken waste has become a serious problem in Malaysia. Flies and an unpleasant odor from poultry coop plague the locals. To decrease the disagreeable odor of chicken manure, the approach used in this study is charcoal as an adsorption method to adsorb an ammonia gas. The sawdust is used as a drying agent for the chicken waste to ensure it produces no smell, and it also contains EM (Effective Microorganisms) as a decomposition mechanism for the bacteria in the chicken waste. The research result obtained positive results based on the number of flies, the odor examined through human surveys, the difference between probiotic and normal chicken waste, and the difference in fat content identified in probiotic chicken. It has been demonstrated by studies that while using wood dust and charcoal can aid in eliminating the odor of chicken droppings, processing chicken droppings with an EM solution is crucial before the droppings start to smell bad. Implementing an automatic system to manage chicken feed and drink is a recommended approach to ensure the project's success. This will ensure that the chickens obtain the proper amount of food and drink at the time specified. Given that chickens are highly susceptible to extreme heat or cold, the following recommendation is to monitor the cage's temperature and humidity levels. The ammonia odor associated with poultry farming affects not only the environment but also people. People who live close to the area where chickens are raised will feel uncomfortable because of this issue. The probiotic approach is the problem-solving strategy, according to the research. The chickens' digestive tract will be aided using EM solution as a drink and meal, resulting in less offensive chicken faeces.

Comparison of two bedding types on the breeding fecundity of C57BL/6J mice.

One of the favored options for generating complex transgenic laboratory mice is through in-house breeding and management strategies. One consideration in the management of these colonies is how the animals' environment may affect reproductive success. Several aspects of the microenvironment can be controlled or manipulated, including cage type, bedding, enrichment, diet, and temperature and humidity. This study sought to evaluate reproductive outcomes for C57BL/6J mice that were randomly assigned to one of two different bedding types: paper based or corncob bedding. Our hypothesis was there would be no significant difference in reproductive outcomes between the two bedding types. A total of 10 males and 10 females were paired at 45 days of age. Animals were allowed to breed for 15 consecutive weeks. Cages were checked daily for the presence of pups and a pup count was performed at 7 days of age. Weaning occurred at 20 or 21 days of age, at which time a final pup count, pup weight, and sex were recorded. All litters born and pups weaned in the 15-week timeframe were used for data analysis. Statistical analysis compared cannibalization between the two groups and the results showed no statistical difference between groups (p > 0.05). Other parameters analyzed included average litter size, average weaning weight, and number of litters per group. All pups counted at Day 7 survived to weaning age in both groups. We concluded that both bedding types produced similar success regarding breeding fecundity in C57BL/6J mice.

Pemberdayaan Kelompok Tani Unggas Jaya Melalui Teknologi Monitoring Gas Amonia Dan Suhu Kandang Otomatis Berbasis IoT

The Poultry Jaya farmer group is in the city of Kefamenanu, the native chicken farming system of the Jaya Poultry Group is still conventional. One of the main problems faced by this group is the lack of monitoring and controlling the temperature of the cage as well as monitoring the ammonia gas produced by chicken droppings. Temperature and ammonia gas levels have a major impact on chicken growth and health. To overcome this problem, it is necessary to implement Internet of Things (IoT) technology integrated with Android applications. This technology allows automatic and periodic monitoring of cage temperature and ammonia gas levels. Farmers can access information via the Android app and take necessary action if significant changes occur. This community service aims to build tools and systems for automatic temperature control and IoT-based ammonia levels integrated with Android, as well as introducing technology and providing training and assistance to the Unggas Jaya farmer group regarding IoT technology in chicken coop management. With the existence of IoT technology, it is hoped that the behavior patterns of farmers will change to become more modern and efficient. This technology can help improve poultry farming performance, reduce risks, and provide convenience in cage management so that it will bring positive changes to the productivity and welfare of farmers. Keywords: Poultry Jaya farmer group; chicken coop; Internet of Things (IoT)

Physiological Response of Broiler Chicken To Cage Temperature

This study aims to study the physiological response shown by broiler chickens to cage temperatures at different times. A completely randomised design was used in this research, with three treatments and five replications of each. The treatments were the average temperature of the cage in the afternoon (30.15oC) , afternoon (27.04oC), and morning (26.01 °C). Each treatment was repeated six times, and each replication consisted of 5 chickens. The variables measured in this study are respiratory rate, heart rate, and rectal temperature in broiler chickens. If the results of the analysis of variance show that there is a significant difference between treatments, it is continued with the Duncan Multiple Range test. The results showed that the temperature of the cage during the day significantly increased the frequency of breathing of broiler chickens (74.90 times/minute), heart rate (279.50 times/minute), and rectal temperature (41.53 oC) compared to the frequency of breathing (57 times). ,27 times/minute and 55.77 times/minute), heart rate (274.07 times/minute and 272.47 times/minute), and rectal temperature (40.74oC and 40.61oC) In the afternoon and the morning, it can be concluded that the temperature of the cage during the day resulted in the frequency of breathing, heart rate and rectal temperature in broilers which were significantly higher than the temperature of the cell in the afternoon and morning, while the temperature of the cell in In the afternoon, the respiratory frequency, heart rate, and rectal temperature were not significantly higher than the cage temperature in the morning.

Reduction in Cold Stress in an Innovative Metabolic Cage Housing System Increases Animal Welfare in Laboratory Mice.

Housing in metabolic cages can induce a pronounced stress response. Metabolic cage systems imply housing mice on metal wire mesh for the collection of urine and feces in addition to monitoring food and water intake. Moreover, mice are single-housed, and no nesting, bedding, or enrichment material is provided, which is often argued to have a not negligible impact on animal welfare due to cold stress. We therefore attempted to reduce stress during metabolic cage housing for mice by comparing an innovative metabolic cage (IMC) with a commercially available metabolic cage from Tecniplast GmbH (TMC) and a control cage. Substantial refinement measures were incorporated into the IMC cage design. In the frame of a multifactorial approach for severity assessment, parameters such as body weight, body composition, food intake, cage and body surface temperature (thermal imaging), mRNA expression of uncoupling protein 1 (Ucp1) in brown adipose tissue (BAT), fur score, and fecal corticosterone metabolites (CMs) were included. Female and male C57BL/6J mice were single-housed for 24 h in either conventional Macrolon cages (control), IMC, or TMC for two sessions. Body weight decreased less in the IMC (females-1st restraint: -6.94%; 2nd restraint: -6.89%; males-1st restraint: -8.08%; 2nd restraint: -5.82%) compared to the TMC (females-1st restraint: -13.2%; 2nd restraint: -15.0%; males-1st restraint: -13.1%; 2nd restraint: -14.9%) and the IMC possessed a higher cage temperature (females-1st restraint: 23.7 °C; 2nd restraint: 23.5 °C; males-1st restraint: 23.3 °C; 2nd restraint: 23.5 °C) compared with the TMC (females-1st restraint: 22.4 °C; 2nd restraint: 22.5 °C; males-1st restraint: 22.6 °C; 2nd restraint: 22.4 °C). The concentration of fecal corticosterone metabolites in the TMC (females-1st restraint: 1376 ng/g dry weight (DW); 2nd restraint: 2098 ng/g DW; males-1st restraint: 1030 ng/g DW; 2nd restraint: 1163 ng/g DW) was higher compared to control cage housing (females-1st restraint: 640 ng/g DW; 2nd restraint: 941 ng/g DW; males-1st restraint: 504 ng/g DW; 2nd restraint: 537 ng/g DW). Our results show the stress potential induced by metabolic cage restraint that is markedly influenced by the lower housing temperature. The IMC represents a first attempt to target cold stress reduction during metabolic cage application thereby producing more animal welfare friendlydata.

Characterization of ultraviolet B light emitting diodes (UVB LED) irradiation device for Wistar rats as an experimental animal model

Link of Video Abstract: https://youtu.be/-l5AY96hUI8 Background: Light emitting diodes (LED) lamps are used widely in daily because of their many benefits, including their low cost, extended lifespan, low energy consumption, and low environmental impact. The use of LEDs, especially ultraviolet B LED (UVB LEDs) is quite extensive and has been developed for the treatment of skin diseases, irradiating pet reptiles, and conducting research on experimental animals. The use of light for therapy must be done carefully so that unwanted side effects do not occur because light also harms tissue. The purpose of this characterization is to determine the stability of temperature and irradiation of the device we make and to determine the duration of light exposure based on the characterization. Methods: The irradiation device use UVB LEDs with a wavelength of 305-310 nm. The characterization carried out included temperature stability and irradiation stability, with observations every 30 minutes for 10 hours and no replication. Statistical analysis using the Kolmogorov-Smirnov (Asymptotic Approximation) testing technique. The test criteria state that if the probability value > level of significance (alpha (α) = 5%). Results: The characterization results from 10 hours of observation with sampling data taken every 30 minutes showed stable LED temperature and room temperature while unstable cage temperature and irradiance. Conclusion: This device can be considered to use in Wistar rat’ experimental studies using UVB and perhaps developed further. The duration of exposure can be adjusted according to the distance of the object and the dose required.

Design of monitoring system for controlling the Temperature of chicken coops based on Internet of Things by utilizing Thingspeak

Broiler chickens are cattle whose growth is affected by ambient temperature. Inappropriate chicken cage temperatures can affect productivity and cause death in broiler chicken, so setting the cage temperature should be done. The design of this automatic temperature control system used the ESP32 microcontroller as the primary controller, the DHT22 as the enclosure temperature sensor and the ThingSpeak as remote monitoring. The microcontroller shall order if the measured temperature is above the set temperature limit and shall order the LED to light red, if the measured temperature is below the specified temperature limit to light blue, but due to the limitations of the author's LED material uses yellow. The results of the study showed that this automatic chicken coop temperature setting tool can work well to automatically regulate the chicken coop temperature and that the data on the results of this study would be entered into ThingSpeak and printed on the LCD.

IMPLEMENTASI PEMANTAUAN SUHU, KELEMBABAN DAN KADAR GAS AMONIA BERBASIS ANDROID PADA PETERNAKAN KAMBING SAPERA MITRA AGRO ABADI FARM

The partner of this program is a dairy goat breeder Mitra Agro Abadi Farm which is located in Barukan Village, Semarang Regency. The fundamental problem of dairy goat farmers in Barukan Village is related to continuous real time monitoring of temperature, humidity and ammonia gas levels in goat cages. Breeders still use the manual method by estimating changes in temperature and humidity and changes in ammonia gas making the goat cage susceptible to disease in goats so that it affects the production of goat milk produced. Therefore, the PKM team offers a solution to create a system that can monitor temperature, humidity and ammonia gas levels in an Android-based goat pen that can be monitored via the farmer's cellphone, then the monitoring data is sent via the internet and then provides notifications to farmers remotely in real time. via mobile phones so that farmers can provide immediate handling or action if the cage conditions are not in accordance with ideal conditions. The goal to be achieved from this activity is that the Barukan Village goat breeders are IT literate and it is easier to control the condition of the goat cages in a measurable manner. Thus, the quality of goat's milk production also increases

Respons Fisiologis Sapi Pedaging terhadap Pengabutan Air Menggunakan Sprinkler Water

Indonesia is facing the problem of low productivity of cattle. To increase the productivity of cattle producer, it can actually be done by relocating the cattle to the comfort zone by observing the physiological response. The purpose of this research is to evaluate the effects of misting cattle’s environment by using water sprinkler on the physiological response and the microclimate of the cattle’s shed. The design used was a paired T-test for physiological and microclimate response variables with 5 replications and a completely randomized design to determine the difference between body surface temperatures. The observations were conducted before water misting and after water misting. The results showed that treatment of misting water in the afternoon significantly decreased (P<0.05) the variables of cage temperature, humidity, temperature humidity index, rectal temperature, respiratory rate, and heart rate. Water misting in the afternoon in cattle produced a good effect on all physiological response variables.
 
 Keywords: beef cattle, pen microclimate, physiological responses, water sprinkler

Sarm1 Regulates Circadian Rhythm Disorder in Alzheimer's Disease in Mice.

Sarm1 (Sterile alpha and TIR motif-containing 1) is a key protein that regulates neurodegenerative pathologies. Alzheimer's disease (AD) is highly associated with neurodegenerative lesions and biorhythmic disturbances. This study aims to decipher the role of Sarm1 in AD-induced circadian rhythm disturbances and AD progression. Open field and water maze tests were used to assess the cognitive function of mice. Thioflavin-S staining was used to assess amyloid-β (Aβ) plaque deposition in the hippocampus and cortex. Rhythmic waveform of home cage activity and temperature was recorded to evaluate circadian rhythm. Expression of clock molecules including Bmal1 and Per2 in the hippocampus were analyzed using western blot and real-time PCR. Further, HT22 cells with Sam1 knockout were treated with Aβ31-35 treatment to initiate circadian rhythm disorder in the cellular level to assess the changes in Bmal1 and Per2. Our data suggested that Sarm1 deficiency rescued cognitive disorder, decreased Aβ plaque deposition in the hippocampus and cortex, inhibited astrocyte activation, improved circadian rhythm, altered clock molecule expression in the cortex and hippocampus in APP/PS1 mice. Sarm1 attenuates circadian rhythm disturbances and reduces AD progression. These data support the potential use of Sarm1 as a therapeutic target to improve circadian rhythm to impede AD progression.

Laboratory animal handling techniques, basic facilities and care: A review

Animals have been utilized in pharmaceutical scientific research and development for more than 300 years. Pharmaceutical items are a must in order to treat illnesses caused by viruses, bacteria, or homeostatic imbalance, among other causes. Pharmaceuticals must be thoroughly characterized via in vitro, in vivo, and clinical trial investigations in order to be used in humans. It is essential to quantify organism's in vivo using animals whose physiology and genetics are most similar to those of humans. Common animals used for laboratory experimental research are Cats, Rats, Dogs, Mice, Guinea pigs and Monkeys. Handling of animals includes transportation, animal housing, temperature of cages, sanitation, water, and food options etc. Proper animal handling techniques affects the outcome of research study, so it very imperative to study and to practice the basics of animal handling techniques. This review is the small efforts to compile the scientific literature and basic information regarding laboratory animal handling techniques, basic facility and care.

The Effect of Ethanolic Extract of Justicia Secunda on Kidney Function in Female Wistar Rats

Purpose: The kidneys serve as the excretory organ of the body. The assessment of serum creatinine, urea, and electrolytes (Na+, K+, HCO3-, Cl-) are crucial and sensitive biochemical markers that are usually involved in the diagnosis of renal impairments (Agbasi et al. 2010; Tietz, 2008; Yakubu et al. 2003).
 Methodology: Thirty-six animals were grouped into 6, with 6 rats in each group. The animals were housed at room temperature in metal cages. Animals in each group had access to clean water and commercial standard rodent pellets. Group 1 (control group) received distilled water, Groups 2-6 were treated orally with low, middle, and high doses of ethanolic extract of Justicia secunda leaves daily for 42 days.
 Findings: A significant increase in the level of urea concentration was observed compared to the control. From the present study, Na+, K+, Cl-, and HCO3 increased significantly in the moderate and high dose groups.
 Unique contribution to theory, policy and practice: In conclusion, this study indicates that ethanolic extract of Justicia secunda increased the serum level of urea, creatinine, and electrolytes.

Implementation of Lora for Controlling and Monitoring Broiler Cage Temperature

A system for controlling the temperature of broilers cages has been made with LoRa data communication. The purpose of this research is to make a prototype of a control system for monitoring and controlling the temperature of broiler controlled by a microcontroller with LoRa communication. The results of measuring system performance are indicated by the DHT11 sensor reading error occurs when the temperature is 36,37 °C and 38 °C by 1.03%. The device that can connect to LoRa is 320 m in Line of Sight (LOS) conditions and 52 m in Non Line of Sight (NLOS) conditions. Overall the tool can function well. Suggestions for further research, an Internet of Things (IoT) based monitoring system can be added using a mobile application.

Implementasi Smartquail Incubator Pada Peternakan Burung Puyuh Cv. Suga Jaya Barokah, Banyumas

Wlahar Kulon Village, Patikraja District, Banyumas has a community of quail breeders (Coturnix coturnix japonica), one of which is Pak Herman. Partner problems are the microclimate of the cage which is uncomfortable, not yet equipped with precise tools and there is only one fan for the building. This condition causes low egg production and egg shell quality. The optimal environmental temperature for quail production period is 28-32°C. Temperatures that are too high cause the egg shells to be thin and easily cracked and make the quail stressed and die easily. The purpose of this PKM-PI is to increase the productivity of quail using the Smartquail Incubator tool and to apply and develop it in order to achieve economic independence for quail farmers. The method of implementing this program consists of conducting surveys and literature studies, designing and manufacturing tools, calibrating sensors, testing tools, evaluating tools, as well as mentoring and monitoring. The results after applying the Smartquail Incubator tool to quail farmers are that the cage temperature control becomes automatic, the energy needed to manage quail is only 2 hours/day, production increases to 38.2 kg/month, mortality decreases to 3 fish/month, quail egg shell becomes thicker, and uses a hybrid electric energy source, namely a combination of PLN and solar panels. Smartquail Incubator uses a microcontroller equipped with an LCD as a means of regulating and monitoring the temperature conditions of the cage. Smartquail Incubator can be controlled with a Smartphone remotely because it already uses the Internet of Things system. The conclusion obtained is that the Smartquail Incubator is an alternative for quail breeders to increase quail productivity. The development of the Smartquail Incubator helps farmers to achieve economic independence.

Salmon lice (Lepeophtheirus salmonis) in the stomach contents of lumpfish (Cyclopterus lumpus) sampled from Norwegian fish farms: Relationship between lice grazing and operational conditions

Cleaner fish are commonly used as a control measure against salmon lice infestations in salmonid farms. Lumpfish (Cyclopterus lumpus) is the most common cleaner fish species used in Norwegian farms. However, little is known about how different operational, including environmental, conditions affect the salmon lice grazing efficacy by lumpfish. In this paper, we analyse salmon lice (Lepeophtheirus salmonis) in the stomach contents of a large sample of more than 20,000 lumpfish from 80 different Norwegian farms. We investigate the proportion of lumpfish with salmon lice and the mean number of salmon lice in the stomach contents of the lumpfish. We further explore how the salmon lice contents vary with different factors like lumpfish weight, weight of salmonids, salmon lice abundance in the cage, cloud cover, and sea temperature. We find that 3.1% of the 24,693 lumpfish contained salmon lice. Most of the lumpfish with salmon lice in their stomach contents contained few (one or two) lice, while there were a few lumpfish which contained many salmon lice. We find more salmon lice in the stomach contents with increasing abundance of salmon lice in the sea cage, lower weight of the salmonids, and in clear weather. Interestingly, for the relationship between lumpfish weight and salmon lice in the stomach contents, we find increased salmon lice grazing from ca. 5 g up to an optimal weight of ca. 40 g, and then a decrease from 40 g. Surprisingly, we find no relationship between sea temperature and salmon lice per lumpfish. We find more salmon lice in the stomach contents of the lumpfish with comparatively high condition. By studying the factors associated with most efficient salmon lice grazing, our paper contributes to understanding how different operational factors affect salmon lice grazing by lumpfish. For controllable factors, the results thus contribute to guiding the best practice for use of lumpfish as a salmon lice control measure.

Implementation of Maggot Cage Temperature and Humidity Control Using ESP8266 Based On the Internet of Things

Black Soldier Fly (BSF) is a fly that can produce a maggot or larvae that are useful for human life, like a decomposer waste in the form of composting, animal feed, animal oil production, source of chitin, and for the economic incomes of the society. This study aims to develop a device that can be used to control the maggot cage temperature and humidity using the ESP8266 microcontroller based on the Internet of Things (IoT). The benefit of this study is the utilization of nozzle-based water spraying that can be used to maintain the maggot cage temperature and humidity to improve the quality of maggot cultivation results. In this study, the sensor used to read the temperature and humidity on the maggot cage is DHT11, then used a water spraying method to handle the temperature and humidity controlled by using the ESP8266 and online based on the Blynk IoT platform. This study result shows that the device built in this study can maintain the maggot cage temperature between 28 to 30oC and humidity over 60%.

Desain Prototype Alat Kontrol Serta Deteksi Suhu dan Kelembaban Kandang Ayam Broiler Dengan Metode Fuzzy Berbasis IoT

<p><em>In this study, the authors designed the control of temperature and humidity detection in chicken coops using the IoT-based Fuzzy method. Control prototype design and detection of temperature and humidity of broiler cages using IoT-based fuzzy methods, and can be monitored remotely with a smartphone using several components, namely NodeMCU ESP32 as a data processor and controller between the Dht-11 temperature sensor and the output in the form of an LCD to determine the temperature of the cage. directly and also in the form of Smartphone communication data via the internet network with Blynk software. the DHT-11 sensor is in charge of reading the temperature and humidity of the chicken coop, the LCD serves to provide a direct display of the results of the temperature and humidity monitoring data parameters, the blynk application itself is tasked with displaying sensor parameters remotely on a smartphone which in the blynk software can monitor or get notification if the temperature state is crossing a predefined threshold. All components require 24V voltage generated by the 220V SMPS power supply to 24V DC. </em><em>The use of fuzzy rules in temperature and humidity control can regulate the output performance of lights and fans that work according to the specified rules.</em></p>

Oxygenation effects on temperature and dissolved oxygen at a commercial Atlantic salmon farm

As climate change continues to warm oceans and exacerbate deoxygenation, coastal ecosystems and anthropogenic activities that occur there are left vulnerable. Fish cultured in ocean net pens are increasingly subjected to oxygen stress. Aeration and oxygenation have been utilized to improve oxygen conditions in pond aquaculture; however, their use in open ocean net pens is still in its infancy. In this study, liquid oxygen was distributed through the NetOx Net system within sea cages in a commercial Atlantic salmon (Salmo salar) farm located off Saddle Island, Nova Scotia (Canada). Real-time sensors were used to measure temperature and oxygen before oxygenation, between oxygenation and the onset of de-stratification due to mixing, and after mixing had resulted in full de-stratification. Oxygenation likely resulted in the upwelling of cold deep water, lowering the cage temperature by ∼4 ℃ which in turn increased oxygen solubility and decreased fish metabolism (though no significant change in growth was observed); these factors resulted in an overall increase in oxygen concentrations by ∼1.5 mgL−1. Following de-stratification, the effects of the oxygenation system were diluted, and mixing due to oceanographic processes dominated the system. As climate change increases hypoxia in coastal regions, where aquaculture practices continue to expand, oxygenation systems will become commonplace. Therefore, it is important to understand whether they will work in an environment that is open to other elements.

Monitoring chicken livestock process using Vento Application at a farm

Regular temperature monitoring in the poultry industry is necessary to produce high-quality products. However, the traditional methods of these activities are still massively applied. Therefore, a modest process on poultry farms requires temperature monitoring and control. Vento is a climate controller developed by Big Dutchman that is easy to understand and user-friendly. Further, the module provides a handy installation and operation on the farm. Therefore, Vento is suitable to use in hot climates area. Vento performs temperature control by reading the input collected from the DOL114 sensor and the DOL12 sensor. The DOL114 sensor performs temperature and humidity detection in the front area of the cage. At the same time, the DOL12 was installed to obtain temperature information behind the cage. Both functions as input to operate the Heater, but only the DOL114 sensor is used to activate the cooling pad. The temperature value gathered by the DOL114 sensor and the DOL12 sensor will be processed by Vento, resulting in average temperature data. Thus, the data obtained from the sensor will be transferred to the Vento system to operate the output in the form of an exhaust fan. The exhaust fan serves as a tool that removes air and ammonia in the cage so that the cage temperature complies with a predetermined setpoint.

Effect of Different Ambient Temperatures on Reproductive Outcome and Stress Level of Lactating Females in Two Mouse Strains.

Simple SummaryThe optimal temperature for laboratory mice has been under discussion for some time. Current standard temperature is 20 °C–24 °C but it has been suggested to elevate the standard to 30 °C, which is the thermoneutral zone for mice. In this study, the effect of different cage temperatures (20 °C, 25 °C, 30 °C) on reproduction and stress hormone metabolite excretion was evaluated in lactating females of two commonly used mouse strains. Pup loss was higher, and weights of mothers and pups were reduced at 30 °C compared to the lower temperatures. In addition, pups showed increased tail length at weaning under the high temperature (30 °C). There was no difference in stress hormone metabolite excretion in mice between temperature groups. We could not show any detrimental effects of the lower or higher cage temperature on stress hormone metabolite excretion, but found decreased reproductive outcome under the higher temperature.Ambient temperature is an important non-biotic environmental factor influencing immunological and oncological parameters in laboratory mice. It is under discussion which temperature is more appropriate and whether the commonly used room temperature in rodent facilities of about 21 °C represents a chronic cold stress or the 30 °C of the thermoneutral zone constitutes heat stress for the animals. In this study, we selected the physiological challenging period of lactation to investigate the influence of a cage temperature of 20 °C, 25 °C, and 30 °C, respectively, on reproductive performance and stress hormone levels in two frequently used mouse strains. We found that B6D2F1 hybrid mothers weaned more pups compared to C57BL/6N mothers, and that the number of weaned pups was reduced when mothers of both strains were kept at 30 °C. Furthermore, at 30 °C, mothers and pups showed reduced body weight at weaning and offspring had longer tails. Despite pronounced temperature effects on reproductive parameters, we did not find any temperature effects on adrenocortical activity in breeding and control mice. Independent of the ambient temperature, however, we found that females raising pups showed elevated levels of faecal corticosterone metabolites (FCMs) compared to controls. Peak levels of stress hormone metabolites were measured around birth and during the third week of lactation. Our results provide no evidence of an advantage for keeping lactating mice in ambient temperatures near the thermoneutral zone. In contrast, we found that a 30 °C cage temperature during lactation reduced body mass in females and their offspring and declined female reproductive performance.