Caffeine Content In Samples Research Articles

NATURAL CAFFEINE, SOURCES, EXTRACTION, USAGE, AND EFFECT OF CAFFEINE ON HUMAN HEALTH

The primary method of absorbing natural caffeine (1,3,7-trimethylxanthine) is through the consumption of coffee.Natural Caffeine is a white crystalline purine containing methylxanthine alkaloids with a harsh flavour. It is the most often consumed bioactive substance across the world. It is a naturally occurring alkaloid found in over 60 plants, including coffee beans, tea leaves, cola nuts, and cocoa pods. Natural Caffeineis extracted from Coffee arabica beans . It can be found in various plant sources such as Coffee beans, tea leaves ,Cacao Beans , Yerba mate ,Guarana ,Kola nuts. It can have a favourable or harmful impact on human health. Natural sources of caffeine frequently include plant polyphenols (phenol-carboxylic acids, catechins) with known antioxidant properties however, stimulant drinks and dietary supplements frequently contain sugars or artificial sweeteners, which can significantly reduce caffeines effects on oxidative stress.C-14 testconducted for Natural Caffeine by Mangalore University and the University of California. An Inhouse testing method by HPLC has been developed by Bio-Med Ingredients Pvt. Ltd for determining Caffeine content in samples. Caffeine finds many applications, as it is used in dietary supplements ,Vitamin Premixes , Cosmetics and for energy performance benefits including improved physical endurance and reduced fatigue.

Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis

Four different phytopharmaceutical dosage forms for use in weight control programs were analyzed. Two different ground herbal blends and their correspondent infusions, a capsule and a tincture were investigated for the presence of compounds used as adulterants in these products. A capillary electrophoresis (CE) method was developed and validated. The optimized experimental conditions were: BGE, sodium tetraborate buffer 20mM, pH 9.2, voltage applied 30kV, capillary temperature 25°C, injection sample at 0.5Psi during 5s. Ephedrine, norephedrine, caffeine and furosemide were baseline separated in less than 7min; the migration times were found to be 2.65, 2.90, 3.75 and 6.58min, respectively. The analysis showed in sample 3 concentrations of 0.45±0.03mgg−1 (ephedrine), 0.33±0.02mgg−1 (norephedrine), 1.09±0.41mgg−1 (caffeine) and 0.80±0.17mgg−1 (furosemide). Caffeine content in samples 1, 2 and 4 was 0.61±0.06mgg−1, 15.66±1.05mgg−1 and 2.27±0.13mgml−1, respectively. Linearity was obtained in the concentration range of 1–1000μgml−1. Limits of detection (LOD) and quantification (LOQ) were determined as 0.42μgml−1 and 1.40μgml−1 (ephedrine), 0.47μgml−1 and 1.40μgml−1 (norephedrine), 0.12μgml−1 and 0.48μgml−1 (caffeine), 0.22μgml−1 and 0.73μgml−1 (furosemide).The common constituents of the samples did not interfere with the potential adulterants. Repeatability was better than 0.24% RSD for the retention time and 1.43% for the peak area. Intermediate precision was tested by changing the capillary, the day of operation and the operator, in all the cases the %RSD was better than 3.06.