Abstract

The primary method of absorbing natural caffeine (1,3,7-trimethylxanthine) is through the consumption of coffee.Natural Caffeine is a white crystalline purine containing methylxanthine alkaloids with a harsh flavour. It is the most often consumed bioactive substance across the world. It is a naturally occurring alkaloid found in over 60 plants, including coffee beans, tea leaves, cola nuts, and cocoa pods. Natural Caffeineis extracted from Coffee arabica beans . It can be found in various plant sources such as Coffee beans, tea leaves ,Cacao Beans , Yerba mate ,Guarana ,Kola nuts. It can have a favourable or harmful impact on human health. Natural sources of caffeine frequently include plant polyphenols (phenol-carboxylic acids, catechins) with known antioxidant properties however, stimulant drinks and dietary supplements frequently contain sugars or artificial sweeteners, which can significantly reduce caffeines effects on oxidative stress.C-14 testconducted for Natural Caffeine by Mangalore University and the University of California. An Inhouse testing method by HPLC has been developed by Bio-Med Ingredients Pvt. Ltd for determining Caffeine content in samples. Caffeine finds many applications, as it is used in dietary supplements ,Vitamin Premixes , Cosmetics and for energy performance benefits including improved physical endurance and reduced fatigue.

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