The leaf extract of Murraya paniculata (MPL) is used to create cadmium sulphide nanoparticles, or CdS NPs and the reducing and stabilizing agent is plant extract. A crucial capping agent in nano production is played by phytochemicals. Analytical methods such as XRD and FTIR are used to characterize CdS NPs. The biomedical applications of prepared CdS NPs were examined, including their anticancer, antifungal, and antibacterial properties. The antibacterial performance was carried out with S.flexneri, C.perfringens, S.typhimurium, and E.faecalis which were all susceptible to the antibacterial action of CdS NPs. At 150µg/ml, S.flexneri and E.faecalis showed a maximum zone of inhibition is 20mm. In addition, A.niger and C.albicans were used to test the antifungal activities, results shows the concentration of 400 µg/ml CdS NPs inhibited the growth of A.flavus (16mm). By using the NRU assay, it was found that the biosynthesized CdS NPs exhibited cytotoxic action against the MCF-7 cell line. Analysis using the NRU assay revealed that treating cell lines with increasing concentrations of NPs had lethal effects. The 24-hour treatment's IC50 was found to be 153.2 µg/ml.