Cadmium In Blood Samples Research Articles

The Effect of Heavy Metal Pollution on Some Haematological Parameters in Domestic Birds: A Study in Camp2 Village, Akamkpa Local Government Area of Cross River State, Nigeria

This study was conducted to evaluate the concentrations of heavy metals in the blood of birds reared for food in Camp2 Community of Akamkpa, Cross River State, Nigeria. Twelve (12) male indigenous jungle fowls (Gallus domestica), six each from the control site (Ugep) and from Camp2 were sampled for the studies. Venous blood samples from the wings of the blood were collected and used for hematological and metal analysis. After digestion of the blood, heavy metals [cadmium (Cd), Lead (Pb), Mercury (Hg), Chromium (Cr), Arsenic (As), and Nickel (Ni)] were detected using atomic absorption spectrophotometer (AAS). The results indicated that Cd, Pb, As and Hg) levels in birds from camp2 village was significantly higher (p<0.05; p = 0.0070) than those of the Ugep control site. The average value of nickel in the blood of Camp2 birds (4.72+1.32mg/L) was significantly higher (p>0.05; p= 0.003) than that of the Ugep control site (4.22+1.07mg/L). The result shows that nickel, lead and Cadmium in blood samples were significantly (p<0.05; p = 0.023) higher in Camp2 compared to the control site. Hence, these values were above WHO standard (0.1mg/l) and FAO/WHO standards of (0.2mg/kg) while others were within joint FAO/WHO standard limit. The studies show that domestic birds can bioaccumulate heavy metals in their blood, which in turn, presents serious health risks to humans who consume these birds within the communities.

A Study of Nafion‐Coated Bismuth‐Film Electrode for the Determination of Zinc, Lead, and Cadmium in Blood Samples

AbstractIn this article a sensitive differential pulse stripping voltammetry technique on Nafion‐coated bismuth‐film electrode (NCBFE) was studied for the simultaneous determination of zinc, cadmium, and lead ions in blood samples at ultra trace levels. The measurement results were in excellent agreement with those obtained from atomic absorption spectroscopy. Various operational parameters were investigated and discussed in terms of their effect on the measurement signals. Under optimal conditions, calibration curves for the simultaneous determination of zinc, cadmium, and lead ions were achieved, based on three times the standard deviation of the baseline, the limits of detection were 0.09 μg L−1 for Cd(II), 0.13 μg L−1 for Pb(II), and 0.97 μg L−1 for Zn(II) respectively.

SI-traceable certification of the amount content of cadium below the ng g(-1) level in blood samples by isotope dilution ICP-MS applied as a primary method of measurement.

The development and implementation of a method for the certification of cadmium in blood samples at low ng g(-1) and sub ng g(-1) levels is described. The analytical procedure is based on inductively coupled plasma isotope dilution mass spectrometry (ICP-IDMS) applied as a primary method of measurement. Two different sample digestion methods, an optimized microwave digestion procedure using HNO3 and H2O2 as oxidizing agents and a high-pressure asher digestion procedure, were developed and compared. The very high salt content of the digests and the high molybdenum content, which can cause oxide-based interferences with the Cd isotopes, were reduced by a chromatographic matrix separation step using an anion-exchange resin. All isotope ratio measurements were performed by a quadrupole ICP-MS equipped with an ultrasonic nebulizer with membrane desolvator. This sample introduction set-up was used to increase sensitivity and minimize the formation of oxides (less MoO+ interference with the Cd isotopes). Because of the very low Cd concentrations in the samples and the resulting need to minimize the procedural blank as much as possible, all sample-processing steps were performed in a clean room environment. Detection limits of 0.005 ng g(-1) Cd were achieved using sample weights of 2.7 g. The method described was used to recertify the cadmium content of three different blood reference materials from the Community Bureau of Reference (BCR) of the European Commission (BCR-194, BCR-195, BCR- 196). Cadmium concentrations ranged between approximately 0.2 ng g(-1) and approximately 12 ng g(-1). For these materials, SI-traceable certified values including total uncertainty budgets according to ISO and Eurachem guidelines were established.

Electrothermal atomization atomic absorption spectrometry with stabilized aqueous standards for the determination of cadmium in whole blood

The use of aqueous calibration graphs obtained by addition of a mixture of (NH42HPO4 and Mg(NO3)2 was used in order to determine cadmium in blood samples diluted only with Triton X-100, using atomic absorption spectrometry with electrothermal atomization using a L'vov platform and peak-height evaluation. The addition of this mixture to the aqueous standards results in a thermal behaviour of aqueous cadmium similar to that shown by cadmium in a blood matrix. Several reference materials were analysed for cadmium by the proposed method. The results indicate that the accurate determination of blood cadmium is possible.