The aim of the present study was to examine the prevalence, symmetry and configurations of C-shaped canals and radicular groove types in mandibular second molars according to age and sex in a Turkish sub-population using cone beam computed tomography (CBCT). In total, 674 CBCT images (368 females and 306 males, aged 18-76 years) of 1348 mandibular second molars were evaluated. The symmetry, configuration of C-shaped root canals, radicular groove types with age and sex were noted. One sample chi-square test and chi-square test were used to determine statistically significant differences. The statistical significance level was set at p < 0.05. Of the 1348 mandibular second molars evaluated, 10.7% had C-shaped root canals. C-shaped root canals appeared to be significantly more common in females than in males (p < 0.05). C1 (44.4%) was the most frequent configuration in the coronal third, and C2 (44.4%), and C4 (31.9%) were the most frequent configurations in middle and apical thirds, respectively. Type 2 (45.1%) was the most common radicular groove type (p < 0.05). The frequency of C-shaped root canals in mandibular second molars in a Turkish sub-population was considerably high (10.7%). CBCT can aid the detection of C-shaped canals and their configurations. Dentists should consider the possibility of C-shaped canals in root canal treatment of mandibular second molars, as these configurations have implications for biomechanical preparation and filling methods.