C-reactive Protein In Diagnosis Research Articles

Values of White Blood Cell Count and C-Reactive Protein in Diagnosis of Acute Appendicitis

Background and objectives. Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis of acute appendicitis is based on careful history, physical examination, and laboratory investigation. The purpose of this study is to investigate the value of white blood cells (WBC) and C-reactive protein (CRP) in the diagnosis of acute appendicitis. Methods. A retrospective analysis of 350 files of patients who underwent appendectomy was conducted. Selected files were those patients who were admitted to ALmasara clinic Tripoli Libya between Aug 2011 to July 2023, ages of patients ranged from 18 to 80 years. Statistical analysis was performed using SPSS version 26 software. A statistical procedure was implemented to compare the hematologic parameters in two groups (Males and Females) to determine if there was any significant difference between them. Means of WBC, Neutrophil, Lymphocyte and CRP were compared between two groups by an independent one-way analysis of variance (ANOVA). Results. This study consisted of 350 participants who were diagnosed with appendicitis. The results showed that about (69.4%) of appendicitis patients exhibited a high white blood cell count (leukocytosis). Slightly over half (52.6%) of the participants had an elevated neutrophil percentage (neutrophilia). Approximately (64.3%) of the appendicitis patients had decreased lymphocyte percentage (lymphopenia). In relation to CRP values, the study showed that most participants (76.5%) had positive CRP results. Conclusion. Individuals with appendicitis have higher levels of leukocytes, neutrophils, and C-reactive protein, and decreased lymphocyte counts. Elevated WBC and CRP values together provide a better specificity and positive predictive value than individual markers.

Diagnostic accuracy of procalcitonin as a marker of neonatal sepsis

Background: Sepsis is defined as “life-threatening organ dysfunction, caused by a dysregulated host response to infection”. Neonatal sepsis is the most common cause of morbidity and mortality in the neonatal period. Early diagnosis of neonatal sepsis is difficult. Therefore, this study was conducted with the objective to assess the diagnostic accuracy of procalcitonin as marker of neonatal sepsis and its comparison with C-reactive protein. Methods: The present study was a hospital-based descriptive comparative study. A total of 59 neonates were enrolled. All suspected neonates for the sepsis admitted to NICU were enrolled in study on the basis of inclusion and exclusion criteria. A detailed clinical examination was done. Blood sample was collected for procalcitonin, C-reactive protein and blood culture. Statistical analysis was performed. Results: In our study diagnostic accuracy of procalcitonin in diagnosis of neonatal sepsis was sensitivity (88.46%), specificity (87.88%), positive predictive value (85.19%), negative predictive value (90.63%) and diagnostic accuracy (88.14%). Diagnostic value of C-reactive protein in diagnosis of neonatal sepsis was sensitivity (88.46%), specificity (69.70%), positive predictive value (69.70%), negative predictive value (88.46%) and diagnostic accuracy (77.97%). Diagnostic accuracy of procalcitonin is maximum followed by C-reactive protein. Conclusions: In our study all patient with gram negative organism were procalcitonin positive whereas 50% Staphylococcus aureus were procalcitonin positive and in candida positive cases out of 6 cases, 5 (83.3%) were procalcitonin positive.

The Role of Salivary C-Reactive Protein in Diagnosis of Neonatal Sepsis

Background Neonatal sepsis remains one of the leading causes of morbidity and mortality among neonates. Objective to evaluate the role of salivary C-reactive protein (CRP) as a diagnostic marker in neonatal sepsis. Methods This case-control study was carried out on 90 neonates including 45 neonates with symptoms and signs suggestive of neonatal sepsis and 45 healthy neonates as controls. All neonates were subjected to full history taking, thorough clinical examination, laboratory investigations including complete blood count (CBC), serum CRP, blood culture, and salivary CRP. Results Septic neonates showed significantly higher salivary CRP compared to controls; it was significantly associated with positive serum CRP, blood culture, and hematological scoring system (HSS). It was significantly higher in neonates who died compared to those who survived. Twenty one cases with positive salivary CRP showed significant positive correlations with the length of the neonatal intensive care unit stay, total white blood cell (WBC) count, mean platelet volume (MPV), neutrophils to lymphocytes ratio (NLR), serum CRP, HSS, significant negative correlations with gestational age, birth weight, APGAR at 1, 5 min, and platelet count. Receiver operating characteristic (ROC) curve for salivary CRP showed that at cut-off value of 0.135 mg/L, the sensitivity was (81.8%) and the specificity was (75.6%) for detecting neonatal sepsis. Conclusion Salivary CRP was significantly higher in the septic group, and was associated with positive serum CRP. Hence, salivary CRP could be considered a novel non-invasive biomarker for diagnosing neonatal sepsis.

Comparing Procalcitonin to C-Reactive protein in Diagnosis of Craniotomi Associated Infection.

Procalcitonin (PCT) is a glicopeptide of 116 amino acid and precursor of calcitonin hormone. At the present day procalcitonin take attention because of being specific to bacterial infection and also is a new indicator for infection which do not affected from viral infection and Systemic Inflamatuar Response Syndrome. The objective of this study was to show the effect of neurosurgical procedures to procalcitonin levels and to distinguish neurosurgical procedure inflammatory reaction from postsurgical associated infection. We carried out our study in Çukurova University Neurosurgery department between May 2007- December 2007. Total number of 44 intracranial tumor existing patients carried out in the study. One day preoperative and four days postoperative values of procalcitonin, white blood cell, C-reactive protein (CRP) and fever were investigated. Results were analyzed in SPSS 15.0 program. In all infection developing patients of the study procalsitonin levels found over 0.1 ng/ml. According to our results patients with a higher of 0.1 ng/ml procalsitonin values in postoperative second and third need attention to followed carefully for postoperative infection. In our study crp exposed a similar kinetic activity in infection developing and infection free patients. We exposed high levels of crp (CRP>5 ng/ml) in both groups. This predicate that crp can not be used in the following of postoperative infection. We may suggest procalcitonin is superior to other existing infection parameters because of not effecting from surgical associated inflamatuar response and a quicker response to routine used other parameters. We suggest that procalcitonin will be a safe and serious parameter in following neurosurgical associated systemic complications by results of new studies which includes more patients and different surgical procedures.

Combining Stress Speckle Tracking with High-Sensitivity C-Reactive Protein in Diagnosis of Coronary Artery Disease.

Introduction: CAD (coronary artery disease) is a leading cause of death and disability in developed nations. Exercise testing is recommended as a first-line diagnostic test for patients with stable angina pectoris. In addition to myocardial strain, high-sensitivity CRP (hs-CRP) can predict the presence of significant coronary artery disease. Aim of work: The purpose of this study was to demonstrate the utility of 2D-speckle tracking at rest and under stress along with hs-CRP for detection of CAD in patients who were referred to the chest pain unit with stable or low risk unstable angina pectoris. Methods: A total of 108 individuals met the inclusion criteria and gave their written consent to participate in this study. Coronary angiography was performed within 48 h after admission to the chest pain unit. Myocardial strain was recorded at rest and during dobutamine administration. Results: Global longitudinal strain at stress appeared to be moderately correlated with the presence of significant coronary artery disease (CAD); r = 0.41, p < 0.0001. A moderate correlation was also found between global longitudinal strain at stress and the severity of coronary occlusion; r = 0.62, p < 0.0001. With a cut-off value of −19.1, global longitudinal strain under stress had a sensitivity of 74.1% and a specificity of 76.7% for detecting significant CAD. Hs-CRP was significantly higher in patients with manifested CAD. Conclusion: Evaluation of longitudinal strain parameters at rest and under stress may predict coronary artery disease in patients with stable angina pectoris. A measurable Hs-CRP is a potential marker of coronary stenosis. Strain data could assist in diagnosing CAD severity.

Role Of C-Reactive Protein in Diagnosis of Acute Appendicitis

Background: Appendicitis is a common surgical emergency and diagnostic dilemma. Making the correct diagnosis is often difficult as the clinical presentation varies according to the age of the patient and the position of appendix. The objective of this study was to identify clinical applicability of C- reactive protein, as a diagnostic test for appendicitis.&#x0D; Methods: This prospective study was carried out in Federal government Polyclinic hospital, Islamabad from January to July 2019, 114 patients underwent appendectomy for clinically diagnosed acute appendicitis. The decision to operate the patient was given by senior registrar. The blood samples for C-reactive protein were drawn before taking the patient to the operating theatre. Removed appendices were sent for histopathological confirmation of diagnosis. The C-reactive protein was then compared with the results of histopathology to determine its validity. The data was entered and analysed in SPSS 23.&#x0D; Results: The sensitivity, specificity, positive and negative predictive values of C-reactive protein in patients with clinical diagnosis of acute appendicitis were found to be 94%, 78%, 93% and 74 % respectively.&#x0D; Conclusion: CRP is helpful in making diagnosis of acute appendicitis. It is highly sensitive but has a relatively low specificity.&#x0D;

“Study of Estimation of Cerebrospinal Fluid C-Reactive Protein in Diagnosis of Acute Meningitis.”

Objectives:To assess the diagnostic role of CSF C-reactive protein quantitatively in acute meningitisand to evaluate the efficacy of CSF C-reactive protein in differentiating pyogenic meningitis fromnon-pyogenic meningitis.Material and Methods: It is a prospective observational study of total 102children with suspected meningitis allocated into three groups based on initial investigations; group-I Pyogenic meningitis, group-II Non-Pyogenic meningitis and group-III No meningitis (Controlgroup). Quantitative CSF C-reactive protein was detected by the latex agglutination method. Datawere analyzed to establish the diagnostic role of CSF-CRP and to evaluate the efficacy of CSF-CRP indifferentiating pyogenic meningitis from non-pyogenic meningitis.Results: A total of 102 clinicallysuspected meningitis patients were studied. Based on CSF findings, the study population (102cases) was categorized into 3 groups. Group I was pyogenic meningitis consist 53 cases (51.96%).Group II was Non-Pyogenic meningitis consists 27 cases (26.47%). Group III was normal CSFfindings consist 22 (21.56%). 98.1% cases of pyogenic meningitis had elevated CSF-CRP level &gt;1.1μg/ml of CSF. In the case of Non-Pyogenic meningitis, 96.2% were found to have CSF- CRP in therange of 0.05-0.10 μg/ml. The mean value of CSF-CRP in groups I,II and III were 5.57±1.48,0.09±0.042 and 0.01±0.010 respectively. Conclusion: Detection of CSF-CRP provides a newdimension to establish the diagnosis of pyogenic meningitis. It is a rapid, reliable and sensitivediagnostic test. From this study it is concluded that CSF-CRP can be used to differentiate pyogenicfrom non-pyogenic meningitis. Early, accurate and appropriate therapy can ameliorate the morbidityand mortality rates in such cases.

Value of C-Reactive Protein in Diagnosis of Septic Arthritis

The diagnosis of septic arthritis may not be clear until a culture has resulted. While the sensitivity of the classic value of 50000 WBC in synovial fluid has been called into question, clinicians will rely on other diagnostic markers to assist in making a diagnosis. The C-reactive protein has been suggested as a highly sensitive marker when considering a diagnosis of septic arthritis. [1, 2] One of these studies have suggested a mean CRP value of septic arthritis of 13. The other suggested a cutoff of 20 to have a sensitivity of 92%. This study set out to confirm the usefulness of CRP in aiding a clinician with a patient who may have septic arthritis.

PREDICTIVE ROLE OF CERULOPLASMIN AND HIGH SENSITIVITE C-REACTIVE PROTEIN AS INFLAMMATORY MARKERS IN ATHEROSCLEROTIC CORONARY ARTERY DISEASE PATIENTS

Background: Coronary artery disease (CAD) remains the leading cause of morbidity and mortality in the industrialized world. The root cause of CAD is mainly atherosclerosis. It seems to be a chronic inflammatory condition, which later develops into an acute clinical event by the induction of plaque rupture, causing thrombosis. Ceruloplasmin is the major copper-carrying protein in the blood and in addition plays a role in iron metabolism. Objective: To assess the predictive value of ceruloplasmin and high-sensitivite C-reactive protein in diagnosis of atherosclerotic coronary artery disease. Patients and methods: The study was conducted on 60 patients presented with clinical features of coronary artery diseases, admitted to Cardiology Department at Sayed Galal University Hospital, throught the period from July 2017 and March 2020, and they were classified according to coronary angiography into 3 subgroups (one, two, multi-vessel disease). They were compared with 20 healthy individuals with identical demographic characteristics not suffering from any disease. Serum ceruloplasmin and serum hs-CRP levels were measured in both groups. Results: A significant increase in serum ceruloplasmin and hs-CRP levels were observed in all patients of coronary artery disease as compared to control group (p=<0.001). Conclusion: In patients of CAD, the serum ceruloplasmin and hs-CRP levels rise due to their property as acute phase proteins.

Role of Combined Blood Neutrophil- Lymphocyte Ratio and C-reactive Protein in Diagnosis of Spontaneous Bacterial Peritonitis

Role of Combined Blood Neutrophil- Lymphocyte Ratio and C-reactive Protein in Diagnosis of Spontaneous Bacterial Peritonitis

The value of C-Reactive protein in diagnosis of acute appendicitis - A prospective study

The value of C-Reactive protein in diagnosis of acute appendicitis - A prospective study

The value of C-Reactive protein in diagnosis of acute appendicitis - A prospective study

The value of C-Reactive protein in diagnosis of acute appendicitis - A prospective study

Role of total leukocyte count and C-reactive protein in diagnosis of acute appendicitis

Background: Acute appendicitis is still one of the most common surgical emergencies. This study was conducted to check the sensitivity and specificity of Total leukocyte count (TLC) and C Reactive Protein (CRP) of acute appendicitis. Aim and objectives were to check the sensitivity and specificity of total leukocyte count in diagnosis of acute appendicitis and to check the sensitivity and specificity of CRP in diagnosis of acute appendicitis. To determine TLC and CRP efficacy in diagnosis of acute appendicitis.Methods: TLC and CRP were calculated in all patients who were planned to undergo appendectomy. Appendectomies were performed independent of results of TLC and CRP levels. For statistical purpose the patients were assigned into 2 groups- Group-A Patients with inflamed/perforated/gangrenous appendix, Group-B patients with Normal appendix. The normal TLC and CRP values, raised TLC, raised CRP, and raised both TLC and CRP values calculated in each of these groups. Sensitivity and Specificity were calculated. The cut off value for TLC was 11x106/L. This value was selected arbitrarily as it corresponds to the elevated TLC. The CRP levels were calculated and cut off value was taken as 1.7mg/dl.Results: Out of 75 cases, 55 were male and 20 were female. Age ranged from 13 to 58 years. CRP was raised in 82.14% of inflamed appendix cases TLC was raised in 76.79% of inflamed appendix cases and both was raised in 92.5% of inflamed appendix cases.Conclusions: TLC and CRP are useful in diagnosis of acute appendicitis. Negative appendicectomy rate can be decreased.

CLINICAL SIGNIFICANCE OF PROCALCITONIN AND C-REACTIVE PROTEIN IN THE PREDICTION OF CARDIOVASCULAR COMPLICATIONS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS

Objective: This work was delineated to assess procalcitonin (PCT) and C-reactive protein (CRP) as prognostic markers for cardiovascular complication in type 2 diabetic patients.Methods: Forty diabetic patients without cardiovascular disease (CVD), 40 diabetic patients with CVD, and 20 healthy control counterparts were participated in this study. Serum PCT and CRP levels were assayed and correlated with metabolic parameters. Receiver operating characteristic (ROC) curve analysis was done for each biochemical marker.Results: The mean level of PCT was 707.17±99.19 ng/l in diabetic patients versus 881.30±123.56 ng/l for the cardio-diabetic patients (p&lt;0.0001). The mean value of CRP was 34.43±17.27 mg/l in diabetic patients versus 50.32±20.19 mg/l for the cardio-diabetic patients (p=0.0003). PCT levels were significantly amplified in the cardio-diabetic patients with increasing CRP, triglycerides (TG), fasting blood glucose (FBG), and cholesterol (p=0.004, 0.0005, 0.002, and 0.01, respectively). CRP levels were significantly enhanced in the cardio-diabetic patients with increasing TG, FBG, cholesterol, and microalbumin (p=0.002, 0.047, 0.003, and 0.001 respectively). ROC curve analysis for PCT and CRP revealed that the area under curve (AUC) was 0.878 and 0.727, respectively. These findings indicate the good validity of the above biomarkers especially PCT as a prognostic marker for cardiovascular complication in type 2 diabetic patients.Conclusion: This study evidences the usefulness of measuring serum levels of PCT and CRP in diagnosis of cardiovascular complication in type 2 diabetic patients.

Assessment of Urinary Interleukin-18 and Serum Amyloid A Efficacies against C-Reactive Protein in Diagnosis and Follow-up of Neonatal Sepsis

Objective: infection is a leading cause of morbidity and mortality in neonates. The aim of this study was to evaluate the diagnostic and prognostic performances of urinary interleukin-18 (uIL-18) and serum amyloid A (SAA) in neonatal sepsis parallel to C- reactive protein (CRP). Subjects and methods: A total of 275 neonates were included in this case-control study. This study was conducted in neonatal intensive care unit at both Minia University Hospital for obstetric and pediatrics as well as Qena University hospital (Egypt). Among those 275 neonates, 150 non septic neonates - who had neither clinical signs nor laboratory findings suggestive of sepsis - were involved as a non-septic control group (Group II) and 125 septic neonates were classified as a septic group (group I). Blood and urine samples were obtained before initiation of antibiotic therapy. Full sepsis screen was performed at the time of sepsis onset plus measurement of SAA and uIL-18 by Enzyme Immune Assay (EIA). A second blood and urine samples were collected 72 hours later. The effectiveness of these 3 biomarkers as diagnostic ones was determined by using receiver operating characteristic (ROC) curve analysis. We also calculated the sensitivity, specificity, and positive, and negative predictive values. Results: The levels of uIL-18 and SAA were significantly elevated in septic neonates than in control group. The two markers showed significant decrease in their levels after 72 hours which matched with clinical improvement but not CRP. Moreover, very high levels of these markers were observed in neonates who died later on. The area under ROC curve (AUC) was used to evaluate the diagnostic efficacies of these markers. The superiority of SAA over both uIL-18 and CRP was obvious in the diagnosis of neonatal sepsis [AUC: 0.995] versus [AUC: 0.934 (uIL-18) and 0.871 (CRP)]. However in discriminating late onset sepsis (LOS), both uIL-18 and SAA have equal diagnostic capacities [AUC: 44 0.991] which are better than that of CRP [AUC: 0.866]. As to EOS, SAA has the most efficient performance as well but CRP is advanced than uIL-18. The specificities and sensitivities of either SAA or uIL-18 were higher than that of CRP in differentiating neonatal sepsis mainly LOS from controls. Conclusion: Both uIL-18 and SAA have enhanced performance over CRP for distinguishing neonatal sepsis mostly for LOS. Thus, they could be promising biomarkers for the screening and follow up of neonatal sepsis. This warrants further assessment of their prognostic values.

Role of Serial C-reactive Protein (CRP) in Relation to Total Leucocyte Count, Platelet Count &amp; Blood Culture for early Diagnosis of Neonatal Septicemia in Developing Countries

Neonatal septicemia is a clinical syndrome of systemic illness accompanied by bacteremia occuring in the first 28 days of life. Neonatal septicemia is one of the major causes of neonatal death in developing countries. Early diagnosis and treatment can prevent neonatal mortality and morbidity. The present study includes: 1) usefulness of CRP (C-reactive protein), Total Leucocyte Count, Platelet Count and Blood Culture in early diagnosis of Neonatal Sepsis, 2) significance of serial CRP in diagnosis of neonatal sepsis. 3) the prognostic value of CRP in neonatal sepsis. This is a prospective study done in neonatal ward, Chittagong Medical College Hospital and carried out from January 2008 to January 2011. Sample size was 300. One hundred fifty neonates with suspected sepsis as cases and 150 healthy babies as control were enrolled in this study. Seventy two percent of cases neonates were preterm and low birth weight. Common risk factors for neonatal septicemia which were identified in this study; preterm (72%), low birth weight (72%), premature rupture membrane (60%), chorioamnionitis (26%) and maternal urinary tract infection (16%) . Out of 150 cases of suspected neonatal sepsis total 80.7%% had raised CRP, in initial sample 70.39% were CRP positive and in 2nd sample additional 9.31% case were CRP positive . In control group 91% were CRP negative. CRP was positive in 100% of culture proven sepsis. Sensitivity of CRP was 80.67% and specificity of CRP was 76.44%. Leucocytosis was observed in 7% of cases and leucopenia was found in 11% of cases. In 82 % cases leucocyte count was found normal. In control group, 95% had normal leucocyte count and 5% had leucocytosis but no leucopenia. Sensitivity of leucocyte count was 18% and specificity was 20.68%. Thrombocytopenia was found in 28% of case group. Out of 150 cases only 15.33% yielded growth of organisms in blood culture. Klebsiella was the most common pathogen isolated which was followed by E.coli and Strph. aureus. Sensitivity of blood culture was 15.33% and specificity was 100% Therefore serial CRP can be taken as alternative method for diagnosis of neonatal sepsis specially in developing countries where blood culture is not readily available.J Bangladesh Coll Phys Surg 2014; 32: 61-65

Procalcitonin and C-reactive protein in early diagnosis of neonatal sepsis

ABSTRACT Background Blood culture is the gold standard for diagnosis of neonatal sepsis, however culture negative sepsis is suspected by high C-reactive protein (CRP) and procalcitonin (PCT) levels. The aim of this study was to compare the diagnostic accuracy of PCT as compared to CRP in diagnosis of neonatal sepsis. Methods Blood samples of 200 neonates with 98 sepsis group and 102 non-sepsis group were analysed for CRP, PCT and culture from December 2012 to July 2013. Sensitivity, specificity, positive and negative predictive values, and diagnostic accuracy of PCT was determined compared to CRP. Results The study group included 56% male and 44% female babies. CRP was raised in 65/98 whereas PCT in 76/98 in sepsis group and in non sepsis group it was 24/102 for CRP and 21/ 102 for PCT. Sensitivity, specificity, positive predictive value, and negative predictive value of PCT was 77.55%, 79.41%, 78.35% and 78.64% respectively as compared to 66.33%, 76.47%,73.03%, 70.27% for CRP respectively. Diagnostic accuracy of PCT was higher at 78.5% as compared to 71.5% for CRP. Conclusion PCT is a better diagnostic marker for early diagnosis of neonatal sepsis as compared to CRP, where blood culture though a gold standard for diagnosis of neonatal sepsis, shows growth in only around 23% of sepsis cases. Diagnosis of neonatal sepsis by blood culture takes up to 72 h and hence use of PCT as a diagnostic marker can minimise this delay in diagnosis. Copyright © 2014, Indian Journal of Medical Specialities. Published by Reed Elsevier India Pvt. Ltd. All rights reserved.

Sensitivity and specificity of CRP as a parameter of atherosclerosis in patients with type II diabetes mellitus

Background: Type 2 diabetes mellitus (T2DM) is an inflammatory atherosclerotic condition with high prevalence of cardiovascular disease. Elevated C-reactive protein (CRP) predicts cardiovascular events in T2DM patients. Objectives: Our work aimed to assess sensitivity and specificity of CRP in diagnosis and follow up of carotid atherosclerosis in T2DM patients (detected by measuring carotid intima-media thickness CIMT). Design and setting: It is observational cross sectional study on T2DM patients from outpatient clinic of fayoum university hospital. Subjects and methods: Hundred patients with T2DM were included; Group I: statin un-treated, Group II: statin treated, and fifty healthy control persons. Laboratory measurement of total cholesterol, LDL, HDL, and serum CRP levels and carotid duplex scanning were done. Results: The patients had higher levels of CRP and CIMT than control. There was strong correlation between CIMT and CRP level among group I and II. LDL in diagnosis of atherosclerosis disease illustrated sensitivity (80%) and specificity (4%), while HDL sensitivity (96%) and specificity (36%). CRP showed higher sensitivity (98%) and higher specificity (72%). Conclusion: CRP level was an important parameter of atherosclerosis with good sensitivity and specificity regardless the level of LDL and HDL.

Role Of Serial C-reactive Protein (Crp) In Relation To Total Leucocyte Count, Platelet Count &amp; Blood Culture For Early Diagnosis Of Neonatal Septicemia In Developing Countries

Neonatal septicemia is a clinical syndrome of systemic illness accompanied by bacteremia occuring in the first 28 days of life. Neonatal septicemia is one of the major causes of neonatal death in developing countries. Early diagnosis and treatment can prevent neonatal mortality and morbidity. The present study includes: 1) usefulness of CRP (C-reactive protein), Total Leucocyte Count, Platelet Count and Blood Culture in early diagnosis of Neonatal Sepsis, 2) significance of serial CRP in diagnosis of neonatal sepsis. 3) the prognostic value of CRP in neonatal sepsis. This is a prospective study done in neonatal ward, Chittagong Medical College Hospital and carried out from January 2008 to January 2011. Sample size was 300. One hundred fifty neonates with suspected sepsis as cases and 150 healthy babies as control were enrolled in this study. Seventy two percent of cases neonates were preterm and low birth weight. Common risk factors for neonatal septicemia which were identified in this study; preterm (72%), low birth weight (72%), premature rupture membrane (60%), chorioamnionitis (26%) and maternal urinary tract infection (16%). Out of 150 cases of suspected neonatal sepsis total 80.7%% had raised CRP, in initial sample 70.39% were CRP positive and in 2nd sample additional 9.31% case were CRP positive . In control group 91% were CRP negative. CRP was positive in 100% of culture proven sepsis. Sensitivity of CRP was 80.67% and specificity of CRP was 76.44%. Leucocytosis was observed in 7% of cases and leucopenia was found in 11% of cases. In 82 % cases leucocyte count was found normal. In control group, 95% had normal leucocyte count and 5% had leucocytosis but no leucopenia. Sensitivity of leucocyte count was 18% and specificity was 20.68%. Thrombocytopenia was found in 28% of case group. Out of 150 cases only 15.33% yielded growth of organisms in blood culture. Klebsiella was the most common pathogen isolated which was followed by E.coli and Strph. aureus. Sensitivity of blood culture was 15.33% and specificity was 100% Therefore serial CRP can be taken as alternative method for diagnosis of neonatal sepsis specially in developing countries where blood culture is not readily available.&#x0D; JCMCTA 2013 ; 24 (2): 22-25

AB0646 Intra-articular level of c-reactive protein in diagnosis of prosthetic knee infection

BackgroundPeriprosthetic joint infection is a rare but potentially serious complication. In 2012, Parvizi et al. suggested intra-articular C-reactive protein (CRP) could be used as a diagnosis marker of periprosthetic joint...