The goal of this study is to show how velocity, temperature, solutal and microorganism slip effects coupled with suction/injection affect mixed convective fluid flow through a vertical cone containing gyrotactic microorganism. To execute numerical computations, the controlling partial differential equations which comprise steady momentum, energy, conservation of mass and motile microorganism balances are initially reduced to a collection of linked non linear ordinary differential equations by similarity transformations, utilizing the MATLAB bvp4c scheme to solve numerically. The current study’s findings have been graphically compared to those of earlier research, revealing a high level of compatibility. The flow fields are numerically exhibited for diversified parameters. For some precise parameter values, dual solutions can be discovered throughout the domain of free, mixed and forced convection and only in the free convection zone do dual solutions exist beyond a critical point. This research has applications for microbial fuel cells, a green sustainable technology for the production of bioelectricity and the treatment of wastewater.