Butyric Research Articles

Diethyl ethylphosphonate retardants disturbed the gut microbiome and metabolite SCFAs in vitro based on simulator of the human intestinal microbial ecosystem

Diethyl ethylphosphonate (DEEP) as a novel organophosphorus flame retardant received increasing attention and its structure was discovered. But there are currently insufficient studies on how DEEP exposure affects the gut microbiome. In this study, the effects of DEEP on the structure and function of the human gut microbiota were examined using the SHIME system. Results from high-throughput sequencing of the 16S rRNA gene show that the high dose DEEP exposure reduced the Shannon and Simpson index in the transverse and descending colon. The Bacillota had the highest proportion while the proportion of Proteobacteria gradually decreased at the phylum level. The abundance of Escherichia, Prevotella, and Bilophila at the genus level increased with increasing doses of DEEP exposure. On the contrary, the abundance of Megasphaera, Klebsiella, and Phascolarctobacterium decreased. The short-chain fatty acids had a significant shift. With increasing doses of DEEP exposure, the concentration of acetic acid and propionic acid increased, while the concentration of butyric acid reached the highest at the medium dose of exposure. In addition, Bilophila, Psychrobacter, Escherichia, and Nostoe showed strong beneficial associations with acetic and propionic acids under DEEP exposure. Phocaeicola, Agathobacter, Klebsiella, Megasphaera, Phascolarctobacterium, and Bacteroides were negatively association with acetic and propionic acids. In a word, the study verified that exposure to different doses of DEEP can cause changes in the composition of the gut microbiome and metabolite SCFAs, which provides ideas for the investigation of other potential hazards of DEEP on human beings.

Butyrolactone-I from marine fungi alleviates intestinal barrier damage caused by DSS through regulating Lactobacillus johnsonii and its metabolites in the intestine of mice

Butyrolactone-I (BTL-1), a secondary metabolite from the marine fungus Aspergillus terreus, exhibits numerous biological activities. Previous research has indicated that Butyrolactone-I alleviates intestinal epithelial inflammation via the TLR4/NF-κB and MAPK pathways. However, the mechanisms underlying its protection against intestinal barrier damage remain unclear. This study aims to further elucidate these mechanisms. We observed that BTL-1 administration increased the abundance of Lactobacillus johnsonii (LJ) in both in vivo and in vitro experiments, prompting an investigation into the effects of LJ and its metabolites on DSS-induced inflammatory bowel disease (IBD). The results demonstrated that BTL-1 significantly upregulated tight junction (TJ) and adherens junction (AJ) proteins, maintained intestinal barrier integrity, and alleviated DSS-induced IBD in mice. These effects were associated with the proliferation of LJ and its metabolites, such as butyric and propionic acids, and the inhibition of the MAPK signaling pathway in the colon. Interestingly, administering LJ alone produced a protective effect against DSS-induced IBD similar to that observed with BTL-1. Furthermore, butyric acid, a metabolite of LJ, also upregulated TJ/AJ proteins in intestinal epithelial cells through the MAPK signaling pathway. Our findings suggest that BTL-1 regulates intestinal flora, promotes LJ proliferation, protects intestinal barrier integrity, increases the concentrations of butyric and propionic acids, and ultimately inhibits the activation of the MAPK signaling pathway in mice to alleviate IBD. Therefore, BTL-1 could potentially be used as a natural drug to prevent IBD and maintain intestinal flora balance. ImportanceWe explored how butyrolactone-I exerts a preventive effect on IBD through intestinal bacteria (Lactobacillus johnsonii).

Insulin Regulation of Lysine and α-Aminoadipic Acid Dynamics and Amino Metabolites in Women With and Without Insulin Resistance.

Insulin is a key regulator of amino acids (AAs) metabolism. Many plasma AAs, including lysine and its metabolite, α-aminoadipic acid (α-AA), a predictor for developing diabetes, are elevated in insulin resistance. In 18 insulin-resistant (IR) over-weight women with polycystic ovary syndrome compared to 12 lean controls, high physiological insulin during a euglycemic clamp failed to normalize many elevated AA metabolites, including branched-chain and aromatic AA, alphaamino- butyric acid, and lysine, but normalized α-AA. To understand the underpinning of differential responses of lysine and its metabolic product α-AA to high physiological insulin in IR compared to controls, we developed a kinetic model utilizing [α-15N1] lysine and [13C1] α-AA as tracers and measured the two tracers simultaneously in α-AA by innovative mass spectrometry. High insulin increased lysine conversion to α-AA in IR and controls but failed to normalize plasma lysine concentrations in IR due to a decrease in lysine metabolic clearance rate (MCR). In contrast, despite higher conversion rates of lysine to α-AA by high insulin, α-AA concentration decreased in IR because of the sustained greater MCR of α-AA. The abnormal AAs and metabolites, even while on high physiological insulin, could potentially explain many functional derangements in IR.

Reactivation of Epstein-Barr virus by n-butyric acid from Pseudoramibacter alactolyticus induces inflammatory cytokines in periapical granulomas

This study investigates whether latent Epstein-Barr virus (EBV) can be reactivated by n-butyric acid from Pseudoramibacter alactolyticus, and if such reactivation induces expression of interleukin (IL)-1β and IL-6 in periapical granulomas. We analyzed periapical granulomas and healthy gingival tissues to detect the presence of EBV and P. alactolyticus. The concentration of n-butyric acid in P. alactolyticus culture supernatants was measured. BZLF-1 luciferase assays were conducted with or without these supernatants. Immunohistochemical detection of ZEBRA-, IL-1β-, and IL-6-expressing cells was performed in the tissue samples. Additionally, mRNA expression levels of BZLF-1, IL-1β, and IL-6 were quantified and statistically analyzed for correlation. The expression of these mRNAs was also measured in Daudi cells treated with or without the culture supernatants. Both EBV and P. alactolyticus were detected in periapical granulomas, but not in healthy tissues. The concentration of n-butyric acid in the culture supernatants was ∼3.58 mmol/L. BZLF-1 luciferase activity in the presence of the culture supernatants was comparable to that of commercially available butyric acid, whereas no activity was detected without the supernatants. Cells expressing ZEBRA co-expressed IL-1β and IL-6. The mRNA levels of BZLF-1, IL-1β, and IL-6 in periapical granulomas were correlated with the number of EBV DNA copies. Daudi cells treated with the culture supernatants expressed BZLF-1, IL-1β, and IL-6 mRNA, while those without the supernatants did not. The study concludes that EBV can be reactivated by n-butyric acid produced by P. alactolyticus, leading to the induction of IL-1β and IL-6 expression in periapical granulomas.

Laoxianghuang polysaccharide promotes the anti-inflammatory cytokine interleukin-10 in colitis via gut microbial linoleic acid

BackgroundOur previous study found that the polysaccharide from Laoxianghuang (LP), fermented fruit of bergamot (traditional Chinese medicine and food), can alter gut microbiota and regulate short-chain fatty acids (SCFAs) in vitro. Nevertheless, there is a paucity of reports on the impact of LP on gut microbiota in vivo. PurposeTo analyze the structures of LP, investigate the influence of LP on the damaged intestinal barrier in DSS-induced colitis mice, and further explore its potential mechanisms. MethodsWe analyzed the physicochemical properties of purified LP by HPLC, SEM, and FT-IR spectrum. Then, to assess the effect of LP in DSS-induced colitis mice, we observed the damage to the colon tissue, measured inflammatory cytokines and tight junction protein expression through RT-qPCR as well as immunofluorescent staining, and investigated the influence of LP on altering gut microbiota and metabolites using 16 s rRNA sequencing and HPLC-MS/MS. Ultimately, the impact of linoleic acid on inflammatory cytokines was confirmed by the LPS-induced RAW264.7 cells. ResultsLP, mainly galactoglucan, could inhibit weight loss and colon shortening, decrease levels of tumor necrosis factor-α (TNF-α), increase levels of interleukin-10 (IL-10) and the intestinal acetic acid and butyric acid, and promote the expression of tight junction proteins ZO-1 and Claudin-1. Meanwhile, LP enhanced the abundance of beneficial bacteria including Romboutsia, Eubacterium_coprostanoligenes_group, and Akkermansia, and regulated linoleic acid metabolism to increase the linoleic acid level. In vitro cell experiment proved that linoleic acid could elevate the level of IL-10 and inhibit inflammatory responses. ConclusionsOur results suggested that LP effectively alleviated colitis by promoting the anti-inflammatory cytokine interleukin-10 via gut microbiota-mediated linoleic acid metabolism.

Integrative microbiomics, proteomics and lipidomics studies unraveled the preventive mechanism of Shouhui Tongbian Capsules on cerebral ischemic stroke injury

Ethnopharmacological relevanceCerebral ischemic stroke (CIS) is one of the most important factors leading to death and disability, which seriously threaten the survival and health of patients. The intentional flora and its derived metabolites are demonstrated to play vital roles in the physiology and onset of CIS. Shouhui Tongbian Capsules (SHTB), a Traditional Chinese Medicine, could regulate gut microbiota and metabolites. Study has found that SHTB has protective effect on CIS, but the mechanism is still unclear. Aim of studyThis study was designed to evaluate the preventive effects and the mechanism of SHTB on CIS injury. Materials and methodsThe rats were pretreated with SHTB for 5 days, then the middle cerebral artery occlusion/reperfusion (MCAO/R) was established. Neurological deficit score, TTC staining, brain water content, H&E and Nissl staining were preformed to evaluate the preventive effects of SHTB on CIS. The Occludin and ZO-1 were analyzed to evaluate the blood-brain barrier (BBB). 16S rDNA sequencing and LC-ESI-MS/MS-based metabolomics profiling were performed to analyze the gut microbiota composition and short chain fatty acids (SCFAs) profile in gut. Serum lipopolysaccharide specific IgA antibody (LPS-SIgA) and diamine oxidase (DAO), as well as colon Claudin 5 and ZO-1 were analyzed to evaluate the intestinal barrier. Proteomics was used to evaluated the proteins profile in brain. Lipidomics were used to evaluate the brain SCFAs as well as medium and long chain fatty acids (MCFAs and LCFAs). Malondialdehyde (MDA), Total Superoxide dismutase (T-SOD), Glutathione (GSH), Glutathione peroxidase (GSH-Px), Catalase (CAT) and reactive oxygen species (ROS) were assayed to evaluate the oxidative stress in brain. Western blot was performed to evaluate the expression of PPARγ, Nrf2, SLC3A2, SCL7A11, GPX4, ACSL4 and LOX. ResultsSHTB prevented rats from MCAO/R injury, which was confirmed by lower cerebral infarct rate, brain water content, neurological deficit score and nissl body loss, and improved brain pathology. Meanwhile, SHTB upregulated the expression of ZO-1 and Occludin to maintain the integrity of BBB. 16S rDNA sequencing and LC-ESI-MS/MS-based targeted metabolomics found that SHTB increased the abundance of gut microbiota, regulated the numbers of intestinal bacteria to increase the production of Acetic acid, Propionic acid, and Butyric acid, as well as decrease the production of Valeric acid and Hexanoic acid in the gut. Meanwhile, SHTB improved the intestinal barrier by upregulating the protein levels of Claudin 5 and ZO-1, which was confirmed by low concentrations of LPS-SIgA and DAO in serum. Multi omics and spearman correlation analysis indicated that SHTB regulated the abundance of Escherichia-Shigella and Lactobacillus to increase Acetic acid, Propionic acid, and Butyric acid to induce the expression of PPARγ, thereby regulating fatty acid metabolism and degradation, improving lipid metabolism disorders, downregulating lipid oxidative stress, inhibiting ferroptosis, and alleviating brain injury. ConclusionThis study confirmed that SHTB improved the disturbance of fatty acid metabolism in brain tissue by regulating gut microbiota and the production of fecal SCFAs to inhibit ferroptosis caused by lipid oxidative stress and prevent CIS injury, which provided a potential candidate drug for the prevention of CIS.

Impact of eight extruded starchy whole grains on glycemic regulation and fecal microbiota modulation

We selected an array of whole grains (WGs), including highland barley, buckwheat, coix seed, foxtail millet, barley, oats, proso millet and sorghum to determine their carbohydrates profile before and after extrusion-expansion, estimated glycemic index (GI) and fermentation characteristics. Our findings reveal that the extrusion processing can significantly influence the structural properties and digestibility of starch. A notable rise in the total dietary fiber (TDF) content was found in foxtail millet, barley and sorghum (p < 0.05) after extrusion. Digestion results of extruded whole grains (EWGs) suggested that coix seeds, oats, and sorghum after extrusion can better manage glycemic index (GI) values due to low starch hydrolysis degree and higher resistant starch (RS) and slowly digestible starch (SDS) content. EWGs with higher TDF content (HE, FE, SE, OE and BE) demonstrated greater SCFA yield, with extruded highland barley exhibiting a superior capacity for generating propionic acid and butyric acid. Extruded barley, buckwheat, oats, and proso millet significantly boosted microbial diversity. All eight EWGs notably curtailed Bilophila growth and fostered beneficial bacteria such as Prevotella, Megamonas, Megasphaera, Bifidobacterium, Parabacteroides and Phascolarctobacterium. This study provides a robust scientific foundation for the application of extrusion technology in functional WGs products.

Investigating intermolecular interactions of surfactant solutions using femtosecond thermal lens spectroscopy

In this study, we examined the impact of various surfactants (oleic acid, butyric acid, Span-20, and CTAB) on the thermophysical properties of methanol (MeOH) using a femtosecond laser-induced thermal lens (TL) spectroscopic technique. Methanol was chosen for its unique convective properties under intense localized heating, making it a promising candidate for studying photothermal responses. We discovered that the TL characteristics of both pure methanol and methanol-surfactant mixtures are significantly influenced by the molecular interactions and physical properties of the components. Our experimental results indicate that the addition of surfactants notably alters the heat transfer dynamics of the solvent by restricting natural molecular movement and modifying thermal properties, which in turn affect the TL signatures. Beyond the surfactant concentration, we observed that the extent of variation in steady-state TL signals, inflection points, and heat transfer mechanisms depends on the molecular properties of the surfactants, such as chain length, the number of active (hydrophilic) groups per molecule, thermal conductivity, viscosity, and intermolecular interactions. Our TL findings provide a detailed investigation into the effect of surfactants on the photothermal response and heat transfer process of a solvent for the first time.

Modulating the Rhizosphere Medium and Indole − 3−butyric Acid Supply Influence Rooting, Nutrients and Biochemical Constituents and Histological Features of Pedilanthus tithymaloids

AbstractPedilanthus tithymaloids is a popular temperate indoor succulent ornamental plant and is highly marketable. However, the appropriate propagation techniques should be adopted to solve the rooting issues of cuttings. Therefore, the present research was designed to specify the optimal indole − 3−butyric acid (IBA) concentration and rooting medium type for obtaining good rooting and healthy plants. Three IBA levels (0, 1.0, 2.0, and 3.0 g L− 1) and five growing mediums (sand, sand: clay, sand: peat moss, sand: perlite, and sand: vermiculite as 1:1, v/v) were used. The experiment was performed in a randomized complete block design in three replications. The obtained data illustrated that roots number per cutting and rooting percentage reached the maximum values by treating P. tithymaloids cuttings with 3.0 g L− 1 IBA x sand: perlite or sand: vermiculite medium. Cultivating the treated-IBA 3.0 g L− 1 cuttings of P. tithymaloids in sand: perlite showed the most enhancements in plant height, branches number and leaves number, leaf area and herb dry weight. However, the heaviest root dry weight was noticed with IBA 3.0 g L− 1 x sand: peat moss medium. Except sand medium, all other tested media in combination with IBA at 1.0, 2.0–3.0 g L− 1 showed remarkable stimulation effect on chlorophyll content. Sand: vermiculite mixture x IBA at 2.0 g L− 1 was the effective practice for improving carbohydrates percentage. By studying the anatomical changes of the adventitious root of P. tithymaloids plant, it was found that there was an increase in the root diameter, phloem and xylem tissues and xylem vessels diameter due to growing in sand: perlite x 3.0 g L− 1 IBA. In conclusion, dipping the cuttings bases of P. tithymaloids in 3.0 g L− 1 IBA and cultivation in sand: perlite considered the most effective combination practice for better rooting ability and growth and could be advisable for commercial production.

Recovery and fractionation of volatile fatty acids from fermented solutions by electrodialysis: electrochemical characterization of anion-exchange membranes

Electrodialysis can be used to recover charged precursors, such as volatile fatty acids (VFAs), of the biopolymers polyhydroxyalkanoates (PHAs). In general, all VFAs are recovered in a receiver solution, despite their advantageous partial fractionation. Herein, the separation of acetic, propionic, butyric, and valeric acids by electrodialysis was evaluated using three anion-exchange membranes (namely, Ralex AMH-PES, Fumasep FAS-PET-130 and PC200D) applying cell voltages from 0V to 2.88V. The mass transfer mechanisms were evaluated by linear sweep voltammetry and chronopotentiometry. Fumasep showed a slightly greater VFAs fractionation capacity than Ralex, while it was much greater for PC200D due to the presence of tertiary amines in its fixed functional groups. Overall, increasing the operating voltage and/or time reduced the degree of VFAs fractionation with all membranes. The higher percent extraction values and greater VFAs fractionation degrees obtained with the PC200D membrane could enhance PHAs storage efficiency.

Exploring advanced antimicrobial effects of Pediococcus pentosaceus and Lactococcus lactis derived from Bufo gargarizans: In vitro analysis and in vivo evaluation in mice

With the context of increasing global concern about antibiotic resistance, the study presented the antibacterial properties of two novel edible bacterial strains, Pediococcus pentosaceus TE0302 and Lactococcus lactis TE0801, derived from the unique ecosystem of Bufo gargarizans. Significant antibacterial efficacy of these strains, with inhibitory zones of 14.77 mm and 15.26 mm, were confirmed through a multi-omics approach involving agar diffusion assays, mouse models, metabolomics, and genomics. These strains also mitigate pathogen-induced colitis and improve gut microbiota by increasing beneficial bacteria and reducing harmful ones. A crucial investigation is that the bacteria's substantial production of N-butyric acid is linked to antimicrobial effectiveness with a correlation coefficient ≥0.972. Genomic analysis shown that these strains feature diverse biosynthetic pathways similar to established antibiotics like Tetracycline and Vancomycin and contain genes for powerful bacteriocin production, especially Enterolysin_A. Pan-genome analysis indicated the unique gene counts of 124 and 179, greatly enhanced the genomic diversity compared to typical reference strains to emphasize the novelty. This study can facilitate a clear understanding of probiotics' genetic and biochemical properties. It highlighted the pivotal role in providing sustainable solutions to combat antibiotic resistance and improve gastrointestinal health, foreshadowing a new era of food science biotechnology.

Anti-viral Effects of Pavetta indica Methanolic Extract and Acyclovir on Behavioral and Biochemical Parameters in Streptozotocin-induced Alzheimer's Disease in Rats.

Alzheimer's disease is a neurological dysfunction of the brain caused by neurodegeneration and oxidative stress. Some viruses, such as herpes viruses, HSV-1, and HSV-2, are causative agents of Alzheimer's disease and result in β-amyloid peptide and tau protein accumulation in the brain. Some antiviral drugs, such as valacyclovir, acyclovir, and foscarnet, reduce amyloid-beta and P-tau. Pavetta indica leaves are also reported for their antiviral properties. The current study aimed to find out the significance of using Pavetta indica methanolic extract and acyclovir against Alzheimer's disease induced by streptozotocin. Wistar rats received acyclovir and Pavetta indica methanolic extract orally at different dose ranges (50, 150, 450 mg/kg) and (125, 250, 500 mg/kg), respectively. The standard therapy, Rivastigmine (2 mg/kg), was given orally. Intracerebroventricular-streptozotocin produced significant alternations in behavioral assessments, including locomotor activity test, Morris water maze test, and elevated plus maze test. Moreover, intracerebroventricular-streptozotocin ameliorated the antioxidant defense activity by decreasing levels of catalase, superoxide dismutase, and reduced glutathione while enhancing the oxidative stress markers, including malondialdehyde, and total nitrite levels. Finally, the main findings showed that intracerebroventricular-streptozotocin significantly increased the inflammatory marker, tumor necrosis factor-α, and disturbed neurotransmitter mediators, including levels of acetylcholinesterase, glutamate, and γ-amino butyric acid. In a dose-dependent manner, acyclovir and Pavetta indica methanolic extract treatments abrogated the streptozotocin-induced behavioral and neurological abnormalities in rats. The potential therapeutic effects of PIME and acyclovir administration in intracerebroventricular-streptozotocin-treated rats may be attributed to its potential antiviral, antioxidant, and anti-inflammatory effects. The current study suggests that Pavetta indica methanolic extract and acyclovir are promising therapeutic targets against Alzheimer's disease.

Elucidating the hysteresis effect in printed flexible perovskite solar cells with SnO2 quantum dot- and PCBM-based electron transport layers

Recently, flexible perovskite solar cells (FPSCs) fabricated using solution-processed printing techniques have garnered significant attention. However, challenges remain in achieving cost-effective, scalable manufacturing under ambient conditions and ensuring stable, efficient devices. This study focuses on fabricating printed FPSCs using the slot-die coating technique and examines the impact of SnO2 quantum dot (QD) and (6,6)-Phenyl C61 butyric acid methyl ester (PCBM) based electron transport layers (ETLs) on device performance and hysteresis. Experimentally results show that SnO2 QD-based devices exhibited favorable photovoltaic properties but significant hysteresis compared to PCBM-based devices. Numerical simulations have shown that the hysteresis effect in devices is influenced not only by the higher concentration of mobile ions in the perovskite layer of PCBM-based devices compared to SnO2 QD-based devices, but also by the more effective redistribution of these ions during forward and reverse J-V scans. The results provide insights into the behavior of printed FPSCs with different ETLs, contributing to the development of high-performance, hysteresis-free printed FPSCs.

Dietary supplementary with ellagic acid improves the intestinal barrier function and flora structure of broiler chicken challenged with E. coli K88

Ellagic acid (EA) contributes to the immunity and anti-oxidant function of body, whereas there are few reports about its effect on the intestinal health and growth performance of broiler chickens. Hence, the present study was arranged to investigate the effect of dietary supplementary with EA on the intestinal barrier function and flora structure of broiler chickens challenged with Escherichia coli K88 (E. coli K88). A total of 216 healthy 1-day-old, Ross 308 broilers with uniform weight were randomly assigned into three treatment groups, six replicates in each group and twelve birds in each replicate. Broilers in the control (CTR) group and E. coli K88 infected group (ETEC) were fed with the basic diet, and 200 mg/kg EA was supplemented into the diet of the E. coli K88 infected group treated with EA (EAETEC). The animal trial had lasted for 42 days, and the outcomes showed that the ADG and ADFI during the animal trial, the jejunal villi height (VH) and the ratio of VH to crypt depth (CD) tended to be decreased with E. coli K88 treated (P< 0.05). Additionally, the level of serum diamine oxidase (DAO) and intestinal malondialdehyde (MDA) were elevated, the activity of intestinal total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), the mRNA levels in jejunal claudin-1 and occludin were down-regulated with E. coli K88 treated as well as the transcription levels of ileal Mucin-2, aquaporin-3 (AQP-3) and Na+/H+ exchanger proteins-3 (NHE-3) (P< 0.05). In addition, E. coli K88 down-regulated the α-diversity index of cecal flora, the ratio of Bacteroidota to Firmicutes and the relative abundance of Barnesiella were up-regulated and it of Alistipes was descended with E. coli K88 treated (P< 0.05). Beyond that, the content of propionic acid in the cecal chyme was decreased and the amino acid metabolic pathways were inhibited with E. coli K88 challenged (P< 0.05). Additionally, there was a significant positive correlation between the relative abundance of Alistipes and the levels of butyric acid in the caecal chyme and the activity of GSH-Px in the intestine (P< 0.05). Interestingly, dietary supplementary with EA could reshape the intestinal flora structure and alleviate the above negative effects of E. coli K88 on broiler chickens. In conclusion, dietary supplementary with ellagic acid improved the intestinal barrier function and flora structure of broiler chickens challenged with E. coli K88.

Biofilm biofertilizer enhances bioactive compounds including antioxidant activity in hybrid rice grains

Excessive use of chemical fertilizers (CF) in rice cultivation has adversely affected soil and crop productivity and human and environmental health. This has led to the introduction of bio-organic inputs like biofilm biofertilizers (BFBF), which have been shown to increase crop yields and soil quality with reduced CF use. However, the effect of the BFBF on rice grain quality has been understudied. The present study investigated the effect of BFBF on bioactive compounds in rice grains, including antioxidants, which are essential in protecting against diseases related to oxidative stress. The study compared two practices i.e. CF practice and BFBF practice, using six hybrid rice varieties. Harvested grain samples were analyzed for total phenolics (TPC), flavonoids (TFC), and Gamma amino butyric acid (GABA) contents, and antioxidant capacity (TAC). Results showed that the BFBF significantly (p &lt; 0.05) increased TPC, TFC, and GABA in most rice varieties. The TAC was also significantly (p &lt; 0.05) higher (30-200%) in the rice grains produced by the BFBF practice. In conclusion, the BFBF alters the biochemical processes of the rice plant leading to improved medicinal properties showing its potential to produce high-quality rice, which is essential to improve human health.

Effects of Nano Silver and Indole Butyric Acid Application on Growth and Some Physiological Characteristics on Hardwood Cutting of Dalbergia sissoo Roxb

Nanosilver (NS) and indole-3-butyric acid (IBA) can improve cutting performance and subsequent growth. This study was performed in Taqtaq city in a randomized complete block design to study the effects of NS (30, 60, and 90 mg/L) and IBA (50, 100, and 200 mg/L) in addition to distilled water as a control on growth and some characteristics of hardwood cutting of Dalbergia sissoo Roxb. Application of IBA enhanced significantly buds sprouting, where the cutting treated with 50 mg/L IBA sprouted after 35.53 days. IBA at 200 mg/L increased plant leaf area significantly to 204.11 dm2 in comparison to the control cuttings (122.00 dm2). Furthermore, IBA at 50 and 200 mg/L increased the number of leaves to 194.66 and 193.00 leaves/plant, compared to control cuttings (158 leaves/plant). The lowest peroxidase activity (902.00 and 903.30 absorbing units/ g fresh leaves) was observed in the cuttings soaked in 30 and 60 mg/L NS, respectively. Both NS and IBA had a significant effect on macro and microelements in the shoot except Mg and Fe. The shoot content of elements was different in response to NS and IBA applications, whereas the high level of IBA decreased significantly K content (25.80 %) it increased significantly the shoot content of Zn (0.22%). However the lowest concentration of NS (30 mg/L) decreased significantly the Cu content (0.02 %) and increased significantly the shoot content of Mn (0.58%). Root response to NS and IBA also was different, where 90 mg/L NS increased significantly each of K (26.40%) and Zn content (0.68 %), whereas it decreased significantly the root content of Fe (8.78%). The enhancement effects of IBA were more than that of NS on most studied characteristics.

Neuroprotective effects of punicalagin and/or micronized zeolite clinoptilolite on manganese-induced Parkinson's disease in a rat model: Involvement of multiple pathways.

Manganism, a central nervous system dysfunction correlated with neurological deficits such as Parkinsonism, is caused by the substantial collection of manganese chloride (MnCl2) in the brain. To explore the neuroprotective effects of natural compounds, namely, micronized zeolite clinoptilolite (ZC) and punicalagin (PUN), either individually or in combination, against MnCl2-induced Parkinson's disease (PD). Fifty male albino rats were divided into 5 groups (Gps). Gp I was used as the control group, and the remaining animals received MnCl2 (Gp II-Gp V). Rats in Gps III and IV were treated with ZC and PUN, respectively. Gp V received both ZC and PUN as previously reported for the solo-treated plants. ZC and/or PUN reversed the depletion of monoamines in the brain and decreased acetyl choline esterase activity, which primarily adjusted the animals' behavior and motor coordination. ZC and PUN restored the balance between glutamate/γ-amino butyric acid content and markedly improved the brain levels of brain-derived neurotrophic factor and nuclear factor erythroid 2-related factor 2/heme oxygenase-1 and decreased glycogen synthase kinase-3 beta activity. ZC and PUN also inhibited inflammatory and oxidative markers, including nuclear factor kappa-light-chain-enhancer of activated B cells, Toll-like receptor 4, nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 and caspase-1. Bcl-2-associated X-protein and B-cell leukemia/lymphoma 2 protein (Bcl-2) can significantly modify caspase-3 expression. ZC and/or PUN ameliorated PD in rats by decreasing the levels of endoplasmic reticulum (ER) stress markers (p-protein kinase-like ER kinase (PERK), glucose-regulated protein 78, and C/EBP homologous protein (CHOP)) and enhancing the levels of an autophagy marker (Beclin-1). ZC and/or PUN mitigated the progression of PD through their potential neurotrophic, neurogenic, anti-inflammatory, antioxidant, and anti-apoptotic activities and by controlling ER stress through modulation of the PERK/CHOP/Bcl-2 pathway.

Analysis of Main Components of Five Mulberry Varieties in Tropics.

Mulberries (Morus alba L.) contain rich and beneficial nutrients for human health. However, as a temperate adaptive species, high-temperature and high-humidity climate conditions may alter the main nutritional value of mulberries after their intended arrival in tropical regions, which has not yet been reported on. In this study, we analyzed the differences in morphology, sugars, organic acids, free amino acids, and aroma contents of five mulberry varieties in the tropics between two harvesting periods. The results show that the full-ripe fruits of M. laevigata W (TLM) have the longest fruit length (83.67 mm) and highest brix (25.90); meanwhile, full-ripe fruits of M. atropurpurea R (D10M) have the longest fruit transverse stem (20.00 ± 0.577 mm) and single-fruit weight (9.63 ± 0.033 g). Fructose, glucose, and sucrose were the main sugars, and oxalic acid, quinic acid, malic acid, and citric acid were the main organic acids in all varieties; in addition, the sucrose content in mature fruits of M. laevigata W. (BLM) and M. alba L. BZZ (BZM) was significantly higher than other sugars. Twenty free amino acids were detected in all five varieties and asparagine was the main free amino acid. A total of 100 volatile compounds were identified, including 31 esters, 20 aldehydes, 14 hydrocarbons, 11 alcohols, 10 acids, 6 ketones, and 8 others. Although the main components of five mulberry full-ripe fruits were significantly higher than the green-ripe fruits, gamma-amino butyric acid and a few other components were otherwise. The research results show that the tropical climate conditions could increase the main nutritional components of mulberries, but the specific molecular regulatory mechanisms need to be further analyzed.

High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method Development and Validation for Simultaneous Determination of Seven Nitrosamine and Azidomethyl-Biphenyl-Tetrazole Impurities in Losartan.

Nitrosamine-related impurities (N-nitrosomethylamino butyric acid [NMBA], N-nitrosodiethylamine [NDEA], N-nitrosodiisopropylamine [NDIPA], N-nitrosomethylphenylamine [NMPA], N-nitrosodibutylamine [NDBA], N-nitrosodimethylamine [NDMA], and N-nitrosoethylisopropylamine [NEIPA]) and 5-[4'-(azidomethyl)-[1,1'-biphenyl]-2-yl]-2H-tetrazole (AZBT) formed during the manufacture of sartan medicines have been classified into human mutagens and carcinogens after long-term treatment. The study developed a simple, economical but highly sensitive procedure for the simultaneous quantification of seven nitrosamines and AZBT impurities in sartan pharmaceuticals. After extraction with methanol (MeOH) 50%, the compounds were analyzed with a reversed-phase liquid chromatography-tandem mass spectroscopy with atmospheric-pressure chemical ionization (APCI) mode (APCI[+] for nitrosamines and APCI[-] for AZBT), selected reaction monitoring, C18 column, gradient elution with 0.1% formic acid in water and in MeOH, respectively. The validated procedure obtained high extraction efficiency (>90%), wide linear range (0.2-50.0ng/mL NMBA, NDEA, NDIPA, NMPA, and NDBA; 0.5-50.0ng/mL NDMA and NEIPA; 2.0-100ng/mL AZBT), limit of quantification <10% of the acceptance level, recovery range of 85%-115% with relative standard deviation <15% and minimum matrix effects for all impurities. The procedure was applied to test 16 commercial losartan samples. As a result, eight samples contained AZBT within the current regulatory limits, but no nitrosamine impurities were detected in all samples.

Plant growth promoting signatory volatiles emitted by a drought-tolerant bacterium Bacillus altitudinis FD48 and its role in moisture stress alleviation in rice (Oryza sativa L.)

Increasing evidence implies that bacterial volatile organic compounds (bVOCs) play a significant role in plant-microbe interaction. Plant associated bacteria produces plant growth modulating volatiles elicits induced systemic tolerance (IST) in plants against a multitude of abiotic stress. Induction of IST and plant growth promotion by signatory bVOCs of Bacillus altitudinis FD48 against drought are reported in this study. The rice seedlings exposed to bVOCs blends of FD48 showed a one-fold increase in whole plant biomass and auxin content (3 µmol g-1 FW) under induced moisture stress. The effect of bVOCs highly depends on the inoculum load. Higher inoculum quantity (100 µL) is detrimental to plant growth. bVOCs produced by FD48 profiled at different growth intervals in GC–MS-ATD revealed a total of 40 bioactive compounds both under stress (PEG 6000) and non-stressed conditions. Interestingly, potential plant growth-promoting compounds such as 1-Hexanol, 2,3-butanediol, dimethyl disulfide, benzene, butanoic acid, pentadecane, and acetic acid are more pronounced. Few compounds produced under non-stress were found to increase during stress (example, 2,3-Butanediol, and acetic acid). This study unraveled the significant biosynthetic pathways induced by FD48 bVOC blends, such as pyruvate metabolism, tryptophan metabolism, sulfur metabolism, fatty acid biosynthesis, and ethanol degradation that anchors in abating moisture stress. Hence, it can be concluded that PGPB B. altitudinis FD48 produced bVOCs could be potential orchestrators of induced systemic tolerance in plants against moisture stress.