Burn Wound Healing In Rats Research Articles

The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat.

Severe burns often result in an exacerbated inflammatory response, which can contribute to further injury. This inflammatory response may lead to an increased risk of infection, multiple organ failure, and death. This study aimed to investigate the potential of reducing inflammation to enhance burn wound healing in rats using ovine's small intestinal submucosa as a carrier for Wharton's jelly mesenchymal stem cells (WJ-MSCs) and Mineral Pitch (MP). A rat burn model was developed, and the animals were divided into four groups: control group: burn, placebo group: scaffold-treated burn, cell experimental group: WJ-MSCs seeded scaffold-treated burn, and cell and MP experimental group: scaffolds loaded with WJ-MSCs and MP-treated burn. After treating the wounds in the relevant groups and sampling them on days 5, 14 and 21, histological and pathological parameters, and the expression of genes involved in angiogenesis and epithelialization were evaluated. The study results revealed several findings in the burn wounds. These included changes in mast cell populations, a decrease in inflammatory neutrophils and lymphocytes, an increase in fibroblasts and blood vessels, and upregulation of angiogenesis and epithelialization genes. These changes collectively contributed to enhanced wound healing in cell and MP experimental group compared to the other groups. The findings suggest that scaffolds loaded with Wharton's jelly-derived stem cells and MP can serve as engineered tools to modulate inflammatory conditions during the burn wound healing process. These interventions can improve burn wound management and promote better outcomes.

Exploring the potential mechanisms of the ethyl acetate fraction of Hippophae rhamnoides L. seeds as a natural healing agent for wound repair

Ethnopharmacological relevanceSea buckthorn (Hippophae rhamnoides L.) has been designated a "medicine food homology" fruit by the National Health Commission of China due to its nutritional value. In traditional Chinese ethnomedicine, Hippophae rhamnoides L. is commonly used to treat nonhealing wounds such as burns, sores, and gastric ulcers. The aim of this study was to explore the healing effects of the ethyl acetate extract of sea buckthorn seeds (SBS-EF) on burn wounds. Aim of the studyThe primary objectives of this research were to determine the most effective medicinal site of action for treating burns with sea buckthorn seeds (SBS) and to investigate the underlying material basis and mechanisms of their therapeutic effects. Materials and methodsThe effects of different components of SBS-EF on the proliferation and migration of human skin fibroblasts (HSFs) were evaluated via MTT assays, scratch assays, transwell assays, and hydroxyproline secretion analysis. SBS-EF displayed the greatest activity amongst the extracts. Subsequent analyses included network pharmacology methodology, molecular docking studies, ultraperformance liquid chromatography UPLC-Orbitrap-Exploris-120-MS and a severe second-degree burn rat model to investigate the chemical constituents and potential therapeutic mechanisms of the SBS-EF. ResultsIn vitro studies demonstrated the efficacy of SBS-EF in promoting HSF growth and migration. UPLC-Orbitrap-Exploris-120-MS analysis revealed that SBS-EF had ten major constituents, with flavonoids being the predominant compounds, especially catechin, quercetin, and kaempferol derivatives. Network pharmacology and molecular docking analyses indicated that SBS-EF may exert its healing effects by modulating the Wnt/β-catenin signalling pathway. Subsequent in vivo experiments demonstrated that SBS-EF accelerated burn wound healing in rats, increased hydroxyproline expression in skin tissue, facilitated skin structure repair, and enhanced collagen production and organisation over a 21 d period. Additionally, exposure to SBS-EF upregulated WNT3a and β-catenin while downregulating GSK-3β levels in rat skin tissue. ConclusionsThe wound healing properties of SBS-EF were attributed to its ability to enhance HSF growth and migration, increase hydroxyproline levels in the skin, promote collagen accumulation, reduce scarring, and decrease the skin water content. SBS-EF may also provide therapeutic benefits for burns by modulating the Wnt/β-catenin signalling pathway, as evidenced by its effective site and likely mechanism of action in the treatment of burned rats.

Preclinical determination of wound-healing activity of halibut oil cream in rat model of burn wound

This study investigated the effects of halibut oil cream, containing omega-3 fatty acids, vitamins A and D, and hydroxyproline, on burn wound healing in rats. Acute dermal toxicity tests confirmed its nontoxicity. Wistar rats were divided into five groups: a control, a positive control treated with silver sulfadiazine 1% (SSD), and three groups treated with 3%, 9%, and 27% halibut oil cream Formulation (HBOF). The SSD and HBOF groups showed significant healing improvements compared to the control. Histopathological analysis indicated increased collagen production in the HBOF groups, suggesting halibut oil cream’s potential as a topical treatment for burn wounds.

Superoxide dismutase-contained solid lipid nanoparticles: Formulation development and In-vivo evaluation for second-degree burn wound healing in rats

IntroductionSuperoxide dismutase (SOD), a natural enzyme with high antioxidant activity, reduces injury and accelerates wound healing by scavenging superoxide radicals. This enzyme plays an important role in cellular defense against oxidative stress such as burn injury. The aim of this study was to load SOD into solid lipid nanoparticles for the treatment of rat burn wounds. MethodsSolid lipid nanoparticles were prepared by Solvent Emulsification Diffusion method and evaluated for particle size, enzyme activity and enzyme entrapment efficiency. Twenty-seven rats in 3 different groups were induced with deep second-degree burns and then treated with SOD-loaded solid lipid nanoparticles, solid lipid nanoparticles without enzyme, or SOD solution. After the treatment period, the wounds were evaluated macroscopically for the area of healing and microscopically for indices of re-epithelialization, granulation tissue and angiogenesis. ResultsThe optimized SOD-loaded solid lipid nanoparticles showed a particle size of 35–85 ± 2.41 nm, 78.4 ± 4.31 % entrapment efficiency and 90 % initial enzyme activity. Macroscopic examination showed that the best recovery rate belonged to the solid lipid nanoparticle group. Pathological studies also showed that angiogenesis and granulation tissue were significantly better in this group. Compared to the other two groups, SOD-loaded solid lipid nanoparticles showed a significant improvement in pathological factors, particularly angiogenesis and granulation tissue, as well as a faster reduction in the number of inflammatory cells. ConclusionBased on this study, solid lipid nanoparticles could be used as an effective delivery system for SOD in the treatment of second-degree burns.

Resveratrol-Ampicillin Dual-Drug Loaded Polyvinylpyrrolidone/Polyvinyl Alcohol Biomimic Electrospun Nanofiber Enriched with Collagen for Efficient Burn Wound Repair.

The healing of burn wounds is a complicated physiological process that involves several stages, including haemostasis, inflammation, proliferation, and remodelling to rebuild the skin and subcutaneous tissue integrity. Recent advancements in nanomaterials, especially nanofibers, have opened a new way for efficient healing of wounds due to burning or other injuries. This study aims to develop and characterize collagen-decorated, bilayered electrospun nanofibrous mats composed of PVP and PVA loaded with Resveratrol (RSV) and Ampicillin (AMP) to accelerate burn wound healing and tissue repair. Nanofibers with smooth surfaces and web-like structures with diameters ranging from 200 to 400 nm were successfully produced by electrospinning. These fibres exhibited excellent in vitro properties, including the ability to absorb wound exudates and undergo biodegradation over a two-week period. Additionally, these nanofibers demonstrated sustained and controlled release of encapsulated Resveratrol (RSV) and Ampicillin (AMP) through in vitro release studies. The zone of inhibition (ZOI) of PVP-PVA-RSV-AMP nanofibers against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) was found 31±0.09 mm and 12±0.03, respectively, which was significantly higher as compared to positive control. Similarly, the biofilm study confirmed the significant reduction in the formation of biofilms in nanofiber-treated group against both S. aureus and E. coli. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analysis proved the encapsulation of RSV and AMP successfully into nanofibers and their compatibility. Haemolysis assay (%) showed no significant haemolysis (less than 5%) in nanofiber-treated groups, confirmed their cytocompatibility with red blood cells (RBCs). Cell viability assay and cell adhesion on HaCaT cells showed increased cell proliferation, indicating its biocompatibility as well as non-toxic properties. Results of the in-vivo experiments on a burn wound model demonstrated potential burn wound healing in rats confirmed by H&E-stained images and also improved the collagen synthesis in nanofibers-treated groups evidenced by Masson-trichrome staining. The ELISA assay clearly indicated the efficient downregulation of TNF-alpha and IL-6 inflammatory biomarkers after treatment with nanofibers on day 10. The RSV and AMP-loaded nanofiber mats, developed in this study, expedite burn wound healing through their multifaceted approach.

Wound healing effect of nutmeg (Myristica fragrans) cream on second-degree burn in animal model.

Second-degree burn, the most common among burn degrees, underscores the importance of timely and proper treatment in influencing prognosis. Nutmeg (Myristica fragrans), renowned for its potent antibacterial and antifungal properties, also serves as an effective antiseptic for open wounds. The aim of this study was to identify the phytochemical constituents of nutmeg essential oil and analyze the wound healing effect of nutmeg cream on second-degree burns in an animal model. An experimental study with a completed randomized design was conducted on Rattus norvegicus strain Wistar rats with second-degree burn. This study had four groups and each group consisting of four rats: B (burn-treated base cream), B+N (burn-treated 3% nutmeg cream), B+SSD (burn-treated silver sulfadiazine (BSS)), and B+N+SSD (burn-treated 3% nutmeg cream and SSD in a 1:1 ratio). The phytochemical analysis of nutmeg essential oil was conducted by gas chromatography and mass spectroscopy (GC-MS). The burn diameter and burn wound healing percentage were measured from day 0 to 18. One-way ANOVA followed by post hoc analysis using the least significant difference (LSD) was employed to analysis the effect. The phytochemical analysis of nutmeg essential oil found that myristicin, terpinene-4-ol, terpinene, safrole and terpinolene were the most abundant putative compounds in nutmeg essential oil. On day 0, the average burn wound diameters were 1.4 cm in all groups and increases were observed in all groups on day 3. The wound diameter decreased until day 18 with the smallest burn wound diameter was found in the B+N group (0.86±0.37 cm), followed by B+SSD (0.93±0.29 cm). The B+SSD group exhibited the highest percentage of burn wound healing (56.80±14.05%), which was significantly different from the base cream (p<0.05). The percentage of burn wound healing in rats given 3% nutmeg cream was 41.88±13.81%, suggesting that nutmeg cream could promote burn wound healing in rats induced by second-degree burns.

The healing effect of a mixture of Arnebia euchroma and animal fat on burn wounds in rats in comparison with sulfadiazine.

Thermal burn is a serious cause of morbidity and mortality that affects millions of people worldwide. The aim of this experimental study was to investigate the efficacy of Arnebia euchroma (AE) to treat burn wounds in a rat model. A total of 80 male rats (200-250g) were shaved over the back of the neck (2×3cm2) and a second-degree burn wound was induced at this site under general anaesthesia. The rats were then randomly assigned to one of four groups (each n=20) and the burns were treated daily for 14 days as follows: (1) dressed with animal fat; (2) dressed with sulfadiazine; (3) dressed with a mixture of AE and animal fat; (4) no treatment (control). Five rats from each group were sacrificed on days 3, 5, 9 and 14 post-burn and the wounds were evaluated histologically and immunohistochemically for the expression of interleukin (IL)-1 and IL-6. There was a significant increase at day 3 and decrease on day 5 samples for the expression of IL-1 in the AE plus fat group and IL-6 in the AE plus fat and sulfadiazine groups, compared to the control and fat treatment groups, respectively. Both AE plus fat and sulfadiazine treatments reduced inflammation and granulation tissue formation by day 5 post-burn, while re-epithelialisation commenced by day 9 post-burn. In addition, burns treated with AE plus fat exhibited keratinised epidermis, associated with regular collagen fibres, compared to moderately dense collagen fibres without vascularisation in the sulfadiazine group. These findings suggested that AE plus fat was superior to sulfadiazine in enhancing burn wound healing in rats.

Self-assembled and dynamic bond crosslinked herb-polysaccharide hydrogel with anti-inflammation and pro-angiogenesis effects for burn wound healing

Excessive inflammation and defective angiogenesis can affect burn wound healing. Recently, naturally derived substances with anti-inflammatory and proangiogenic properties have attracted public attention. The design and fabrication of naturally derived substance-based bioactive hydrogels as wound dressings are of interest and important for regulating the complex microenvironment of the wound bed. Herein, we developed a hydrogel by self-assembling a natural herb (glycyrrhizic acid, GA) dynamic Schiff base crosslinking of hyaluronic acid derivatives and integrating deferoxamine (DFO). The naturally derived herbal GA endowed the bioactive hydrogel with a native anti-inflammatory capability. The introduction of dynamic bond crosslinking improved the hydrogel stability. In addition, dynamic crosslinking is conducive for integrating the naturally-derived DFO, delivering it to the wound site, and promoting angiogenesis. Rheological tests, injectability, degradation behavior, and drug release performance demonstrated the enhanced stability of the hydrogel and sustained release of DFO. Cytotoxicity, cell proliferation, and cell migration tests suggested that the hydrogel was biocompatible. Further, the hydrogel exerted anti-inflammatory and angiogenic effects and accelerated burn wound healing in rats. Therefore, the proposed hydrogel has the potential to be a natural, herb-based, bioactive dressing for burn wound management.

Effect of combination of photobiomodulation 904 nm superpulsed laser therapy and Hippophae rhamnoides L. on third-degree burn wound healing.

Burn is a traumatic injury and aesthetic scarless repair poses a great challenge in area of cosmetic dermatology. Focus on multimode therapeutic strategies to promote healing of burns by regulating various stages of healing is warranted. Photobiomodulation therapy (PBMT), a non-invasive modality grabs the attention to repair impaired wounds. Seabuckthorn extract (SBTL-ALE) is known to possess antioxidant, anti-inflammation, and tissue-repair abilities. Current study aims to assess the effect of combination treatment of PBM 904 nm superpulsed laser and SBTL-ALE (2.5%) on repair of third-degree burn in rats. Rats were randomized into five groups: uninjured, control, SBTL-ALE, 904 nm PBMT, and combination. A transdermal burn wound was induced on the dorsal side of rats of all groups except the uninjured group and respective treatment was applied for 7 days postwounding. Dual treatment increased wound area contraction compared to control and either treatment alone. Immunohistochemical analyses exhibited increased angiogenesis, dermal hydration, collagen synthesis, and maintained redox homeostasis as evidenced by enhanced expression (p < 0.05) of CD31, aquaporin3, collagen type 3, Nrf2, and HO1 in combination group compared with control. Conversely, pro-inflammatory and oxidative stress markers exhibited reduced (p < 0.05) TNF-α, IL-6, IL-1β, NOS-2, ROS levels, and increased catalase activity in combined treatment. Furthermore, energy metabolizing enzymes viz. citrate synthase, CCO, and ATP contents were substantially (p < 0.05) increased, and LDH activity was reduced in the combination group. Dual treatment (PBMT + SBTL-ALE) prominently accelerates third-degree burn wound healing in rats, which could pave the path for multimode therapeutic strategies for the management of burns and dermal cosmetic care.

Polysaccharides from South Tunisian Moringa alterniflora Leaves: Characterization, Cytotoxicity, Antioxidant Activity, and Laser Burn Wound Healing in Rats.

Phytochemical properties have recently increased the popularity of plant polysaccharides as wound dressing materials. This work aims at studying the structural characteristics of polysaccharides extracted from Moringa leaves (Moringa Leaves Water Soluble Polysaccharide: MLWSP), and its antioxidant activities, cytotoxic effects, and laser burn wound healing effects in rats. This MLWSP was structurally characterized. Results showed 175.21 KDa and 18.6%, respectively, for the molecular weight and the yield of the novel extracted polysaccharide. It is a hetero-polysaccharide containing arabinose, rhamnose, and galactose. XRD suggested a semi-crystalline structure of the studied polymer and FT-IR results revealed a typical polysaccharide structure. It is composed of 50 to 500 µm rocky-shaped units with rough surfaces and it was found to inhibit the proliferation of the human colon (HCT-116) (IC50 = 36 ± 2.5 µg/mL), breast (MCF-7) (IC50 = 48 ± 3.2), and ovary cancers (IC50 = 24 ± 8.1). The MLWSP showed significant antioxidant effects compared to Trolox (CI50 = 0.001 mg/g). Moreover, promising wound healing results were displayed. The effect of MLWSP hydrogel application on laser burn injuries stimulated wound contraction, re-epithelization, and remodeling phases 8 days after treatment. The wound healing potential of MLWSP may be due to its significant antioxidant activity and/or the huge amount of monosaccharide molecules.

IN VIVO ИССЛЕДОВАНИЕ РАНОЗАЖИВЛЯЮЩЕЙ АКТИВНОСТИ ПОЛИСАХАРИДНОГО ГЕЛЯ С ИНКАПСУЛИРОВАННЫМ ОБЛЕПИХОВЫМ МАСЛОМ (HIPPOPHAE RHAMNOIDES)

Objective: To study the morphophysiological features of burn wound healing in rats using a polysaccharide gel form based on pectin and polysaccharide from apricot gum with encapsulated sea buckthorn oil. Methods: The study was carried out on two groups of laboratory rats with 18 animals in each group. A skin burn injury was inflicted in both groups (for 5 seconds at a temperature of 150°C). In the main group, in order to facilitate regeneration, a new gel form based on a polysaccharide composite with encapsulated sea buckthorn oil was applied to the burn site. In the control group, instead of the gel, a 3% hydrogen peroxide solution was applied to the surface of the wound. The skin, subcutaneous tissue, and muscles were sampled for morphological studies, sample size being 2×2 cm. The tissue was sampled within unchanged skin and processed for histological examination. Histological slides were stained with hematoxylin-eosin and viewed under Axiostar PLUS microscope (Carl Zeiss, Germany). Results: In the control group, physiological wound healing took place which corresponds to incomplete healing (reparative regeneration) and the formation of scars. In the main group after the gel treatment, the formation of granulation tissue within the epidermis, dermis, and subcutaneous fat was accelerated. It was noted that the use of the proposed gel limited destructive changes, and contributed to the steady maturation of young connective tissue, ensuring the process of re-epithelialization and complete closure of the defect Conclusion: The proposed polysaccharide gel with encapsulated sea buckthorn oil has great potential for use as a dressing for burn wounds to expedite their healing. Keywords: Burn wound, wound healing, polysaccharide gel, pectin, apricot gum, sea buckthorn oil (Hippophae rhamnoides).

Stimulation of Burn Wound Healing in Rats by Nanosecond Repetitive Pulsed Microwave Radiation

Stimulation of Burn Wound Healing in Rats by Nanosecond Repetitive Pulsed Microwave Radiation

Burn wound healing effect of mesenchymal stem cells seeded into collagen-chitosan in animal model

Skin burn healing is still a major issue and treatments aim to accelerate the burn healing process to minimize the associated complications. Despite the fact that a wide range of wound dressings have been designed, few of them act well and sufficiently in repairing and protecting burn wounds. This study aimed to investigate the accelerating effect of mesenchymal stem cells (MSCs) seeded collagen-chitosan hydrogel on burn wound healing in rats. In this in vitro study, adipose tissue was obtained from 10 patients undergoing liposuction surgery and MSCs were isolated and identified. Cell viability was measured by the MTT assay method. The cells were examined for apoptosis and expression of surface markers using flow cytometry. Burn wounds were created on the back of rats and the animals were divided into control group (n = 6), and groups treated with MSCs (n = 6), treated with collagen-chitosan hydrogel (n = 6), and treated with MSCs seeded collagen-chitosan hydrogel. The healing process of burn wounds was evaluated quantitatively and qualitatively on days 7, 14 and 21 after treatment. Data were analyzed using ANOVA. The wound closure, and skin re-epithelialization, vascularization, collagen synthesis, thickness, density and elasticity at the transplant site were highly promoted by MSCs seeded collagen-chitosan hydrogel than MSCs or collagen-chitosan hydrogel alone (P &lt; 0.001). Dressing of burn wounds with MSCs seeded collagen-chitosan hydrogels has significant potential to accelerate the process of burn wound healing.

Polysaccharides in CO2 enriched Arthrospira platensis: Structure, physico-chemical properties, antioxidant and cytotoxicity activities and laser burn wound healing in rats.

This work was undertaken to determine the structural characteristics of polysaccharides extracted from CO2-enriched Arthrospiraplatensis (Spirulina Water Soluble Polysaccharide: SWSP), as well as its antioxidant activities, cytotoxic effects and laser burn wound healing in rats. This SWSP was structurally characterized by Scanning Electron Microscopy (SEM), Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), high-performance liquid chromatography (HPLC), and thin layer chromatography (TLC). This novel polysaccharide was found to have an average molecular weight of 6.21 kDa. It is a hetero-polysaccharide composed of rhamnose, xylose, glucose and mannose. According to XRD and FT-IR spectra, the SWSP showed a semi-crystalline structure. It is composed of 100 to 500 µm geometric shaped units with flat surfaces and it was found to inhibit the proliferation of human colon (HCT-116) and breast (MCF-7) cancers. This polysaccharide display potential antioxidant activities determined through three different assays: scavenging activity against 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) scavenging assay and ferric reducing antioxidant power assay (FRAP). Results strongly support the beneficial effects of the SWSP to accelerate wound healing in rats. Indeed, its application significantly increased tissue re-epithelization and remodeling phases, after 8 days of the experiment. Findings herein demonstrated that SWSP could be a novel auspicious source of natural wound healing closure and/or cytotoxic remedy.

Effect of cell-free and platelet plasma on burn wound healing in rats

The presented study analyzes the healing effect of pure cell-free plasma and plateletrich plasma. The study was conducted on Wistar rats. Three groups of rats were formed for the experiment: a control group and two experimental ones (No. 1, No. 2). Burn wounds were inflicted on animals under sevoflurane anesthesia in the interscapular region with a copper-plated disc with a diameter of 20.0 mm heated in the flame of a gas burner to a temperature of 300.0 OC by applying it for 2.0 - 3.0 seconds. In the control group, treatment of burn wounds was not carried out, but surface application of aerosol 10% lidocaine was used to reduce the pain syndrome. For rats of experimental groups No. 1 and No. 2, treatment of burn wounds with plasma was started on the 5thday after injury. Rats of experimental group No. 1 were treated by injecting cell-free plasma under and around the burn. Rats of experimental group No. 2 were treated by injecting platelet-rich plasma under and around the burn. Monitoring and evaluation of wound healing was carried out by measuring their area. Photo fixation and photo documentation were carried out at the time of the burn wound and then on the fifth day after the injury (the first injection of plasma samples), on the 14th day after the injury, on the 23rd day after the injury and on the 30thday after the injury. The result of the study showed that both cell-free plasma and platelet-rich plasma accelerate the healing of a burn wound in comparison with the control. But platelet plasma, in comparison with cell free plasma, has a stronger therapeutic effect, significantly accelerating the contraction of the wound edges in the very first days after administration.

Effectiveness Of Ethanol Extract Of Turmeric (Curcuma Domestic Valet) Gel Against Grade Iia Burn In White Rats (Rattus Norvegicus)

This study aims to determine the acceleration of burn wound healing in rats given ethanol extract of turmeric rhizome (Curcuma Domestica Valet) gel and to see the granulation of the skin histopathology of rats. This research method is experimental with a post-test-only design. The research location is in the UNPRI Bio-Molecular Laboratory with 24 rat samples. Treated with Turmeric Rhizome Extract Gel with Concentrations of 25%, 50%, 75%, and 100% and Silver Sulvadizine as Positive Control and Gel as Negative Control. The results showed that the effectiveness of the best Turmeric Rhizome Extract Gel for wound healing was a concentration of 100% on the 21st day of 15 mm. the worst diameter was the negative control on the 21st day of 20 mm. This happens in the healing of burns because of the content of tannins and flavonoids. Measurement of the granulation tissue diameter on the histopathological picture with the results of a concentration of 100% with a thickness of 863 m. Hypothesis test Ha is accepted There is Effectiveness of Ethanol Extract of Turmeric Rhizome Extract (Curcuma Domestica VALET) in Healing Grade IIA Burns. Suggestion It is necessary to do further research related to the factors that cause weak anti-inflammatory and antibiotic Kunit rhizome Extract Gel against second-degree burns.

Hippophae rhamnoides L. leaf extract diminishes oxidative stress, inflammation and ameliorates bioenergetic activation in full-thickness burn wound healing

BackgroundGlobally, burn wounds are the prominent cause of morbidity and occasionally mortality, besides psychological and financial burden to the patients and health care systems. Numerous medical complexities associated with burn injuries prompted the quest for novel, practical therapeutic healing approaches, which could potentially influence different phases of the repair process positively. PurposeThe present study investigates the influence of Seabuckthorn leaf aqueous lyophilized extract (SBTL-ALE) on full-thickness burn wounds in rats to establish its healing efficacy and to explore its possible mechanisms of action concerning molecular, cellular, and histopathological features. MethodsTransdermal burn wound created on the dorsal surface of the rat. Animals were randomly distributed into three different groups and topically treated with vehicle base (control group), 2.5% SBTL-ALE ointment (experimental group) and silver sulfadiazine cream (SSD, positive drug control group) twice daily for seven consecutive postwounding days. ResultsImmunohistochemical analyses revealed enhanced proliferation, re-epithelialization, neovascularization, dermal hydration as demonstrated by significantly up-regulated (p < 0.05) expression of PCNA, cytokeratin-14, CD31, and aquaporin3, respectively in SBTL-ALE treated group as compared with the control and SSD groups. Picrosirius red staining confirmed the deposition of well-aligned, densely-packed, mature collagen in the SBTL-ALE group. The signature markers of oxidative stress and inflammation (ROS, 3-nitrotyrosine, NOS-2, NF-κB, TNF-α, IL-1β, IL-6) were found to be significant (p < 0.05) decreased in the treated group as compared to the control. Immunoblot analysis exhibited that SBTL-ALE treatment conferred cytoprotection as evidenced by significantly (p < 0.05) up-regulated expression of GRP78, thermo-sensitive cation channel (TRPV3), and HIF-1α. Additionally, activation of energy metabolism and bioenergetics (increased hexokinase, citrate synthase, G6PD, cytochrome c oxidase, ATP, decreased LDH) was observed after SBTL-ALE treatment. ConclusionSBTL-ALE treatment confers cytoprotection, redox homeostasis, cellular proliferation, re-epithelialization, angiogenesis, and dermal hydration during repair of full-thickness burn wound healing in rats. Furthermore, its treatment attenuates oxidative stress, inflammatory responses and enhances bioenergetic activation. Taken together, SBTL-ALE topical application augments burn wound healing, which could be of high clinical significance. The leads from the present findings could serve as valuable information for further applications of SBTL-ALE in pharmaceuticals and cosmeceuticals.

The Effect of Transplantation of Collagen Hydrogel with Adipose-Derived Mesenchymal Stem Cells on Burn Wound Healing in Rats

Background: Cell therapy is one of the most challenging methods in the healing of burn wounds in the world. The present study aimed to determine the effect of collagen hydrogel with adipose-derived mesenchymal stem cells on burn wound healing in an animal model. Materials and Methods: The present study was experimentally performed on 24 Wistar rats. Burn wounds were created on the skin of rats. Then, the rats were divided into a control group treated with normal saline and a group treated with collagen hydrogel with adipose tissue-derived mesenchymal stem cells. The healing process of burn wounds was quantitatively and qualitatively evaluated at intervals of every seven days for 21 days. The obtained data were analyzed in the form of burn wound healing parameters using one-way ANOVA. Results: The wound area was traced on days 7, 14, and 21 in the treatment group which was significantly smaller than that in the control group, indicating a faster wound healing in the treatment group compared to the control one (P&gt;0/05). Conclusion: Mesenchymal stem cells transferred by hydrogel to the wound site rapidly heal burn wounds; therefore, evaluating the use of this dressing in the healing of burn wounds is important in clinical cases.

Superpulsed 904 nm laser photobiomodulation combined with coenzyme Q10 synergistically augment burn wound healing

The management of multifaceted burn wound imparts a huge health care burden. Innumerable complexities associated with non-healing burn wounds led to the search for new therapeutic healing modalities. Earlier, our studies have shown that photobiomodulation (PBM) with superpulsed 904 nm laser increased cellular proliferation, bioenergetics activation, reduced inflammation and nitroxidative stress during burn wound healing. Coenzyme Q10 (CoQ10), a mitochondrial electron carrier acts as a potent antioxidant, maintains redox balance and mitochondrial dysfunction. Multi-target therapy aims to accelerate healing of intractable impaired wounds, which could exhibit a synergistic effect to potentially affecting different phases of the repair process. The present study investigates the efficacy of 904 nm superpulsed laser-mediated PBM (200 ns pulse-width, 100 Hz frequency, 0.24 J/cm2 total energy density, 0.40 mW/cm2 average power density and 19.77 W/cm2 peak power density for 10 min once daily for 7 days post-wounding) and CoQ10 (0.6%, w/v) topical treatments individually and in combination on full-thickness burn wound healing in rats and further explore the underlying mechanisms of action. The dual treatment showed significant (p < 0.05) synergism in enhancing pro-reparative healing markers: wound closure, mitogenesis (DNA, protein), angiogenesis (HIF-1α, VEGF), re-epithelialization, collagen deposition and bioenergetics activation (mitochondrial activity, cytochrome c oxidase and ATP), redox homeostasis (increased Nrf2, HO1, TXNRD2, catalase, SOD and decreased reactive oxygen species, lipid peroxidation levels) as compared to the standalone treatment and burn control groups. The PBM-CoQ10 combination treatment demonstrated significantly (p < 0.05) increased cellular proliferation (TGF-β2), light-sensitive ion channel regulation (TRPV3) and cytoprotection (HSP90, GRP78) during burn wound repair. Collectively, the combined therapy of PBM and CoQ10 is highly effective than either treatment alone in augmenting burn wound healing by efficiently attenuating oxidative stress and enhancing cellular proliferation, collagen deposition and bioenergetics activation. The study shows that combined treatment distinctly augments full-thickness burn repair in rats, which could pave the path for multi-target therapy approaches for non-healing impaired wounds in clinical care.

Hyperglycemia-induced impaired neutrophil activity in the dynamic of burn wound healing in rats

article: Hyperglycemia-induced impaired neutrophil activity in the dynamic of burn wound healing in rats - Minerva Biotechnology and Biomolecular Research 2022 March;34(1):22-33 - Minerva Medica - Journals