Buriti Pulp Research Articles

Impact of pre-exposure stress on the growth and viability of Lactobacillus acidophilus in regular, buriti pulp and orange byproduct fermented milk products

The loss of probiotics viability in yogurts and fermented milk is a significant challenge in producing yogurt and fermented milk. Thus, pre-exposure of probiotics to stress conditions can be a viable alternative to increase the probiotic viability. Moreover, the use of fruit pulp and agro-industrial residues in these products has demonstrated promising results in promoting growth and improving the viability of probiotics. Thus, this study aimed to evaluate the effects of pre-exposure to acid, oxidative and osmotic stress on the growth and viability of Lactobacillus acidophilus in yogurts and naturally fermented milk containing buriti (Mauritia flexuosa Mart.) pulp or orange byproduct. L. acidophilus was individually pre-exposed to acid, oxidative, and osmotic stress and used in the production of yogurts and fermented milk to determine both the acidification profile and growth of the cultures. Furthermore, during cold storage, the post-acidification profiles and viability of microbial cultures added to the yogurts and fermented milk were monitored. Results showed that pre-exposure to stress conditions influenced the growth parameters as the growth rate (μ) and lag phase (λ) of L. acidophilus and the starter cultures of S. thermophilus and L. delbrueckii subsp. bulgaricus. Moreover, an increase in the viability of L. acidophilus - pre-exposed to acid stress - was observed on the 21st day of storage of natural yogurts containing orange byproduct compared with non-stressful conditions. This study reports new data on the growth of probiotic cultures pre-exposed to stress conditions in products added of pulps and agro-industrial residues, which have not yet been shown in the literature.

Ultrasound-Assisted Extraction of Phenolic Compounds from Buriti (Maurtitia Flexuosa L. f.) Pulp

Little has been discussed about the content and profile of phenolic compounds from buriti. The few studies found in the scientific literature diverge about these levels. So, aiming to propose a standardization in the method of extraction of phenolic compounds from buriti pulp and to facilitate the comparison between the results, this work aimed to validate the methodology of extraction of these compounds through an asymmetric factorial design. The optimal conditions for extraction of phenolic compounds in buriti pulp were evaluated in an asymmetric factorial study with three factors (solvent, number of extractions, and time). The extraction procedure consisted of adding the specific solvent to the sample, and submitting it to an ultrasound bath for different times. We concluded that the best treatment was the extraction with acetone in three steps of 30 min each, solid-liquid ratio of 1:10 (g:mL, m/v), using an ultrasonic bath at room temperature.

Functional Fermented Milk with Fruit Pulp Modulates the In Vitro Intestinal Microbiota.

The effect of putative probiotic fermented milk (FM) with buriti pulp (FMB) or passion fruit pulp (FMPF) or without fruit pulp (FMC) on the microbiota of healthy humans was evaluated. FM formulations were administered into a simulator of the human intestinal microbial ecosystem (SHIME®) to evaluate the viability of lactic acid bacteria (LAB), microbiota composition, presence of short-chain fatty acids (SCFA), and ammonium ions. The probiotic LAB viability in FM was affected by the addition of the fruit pulp. Phocaeicola was dominant in the FMPF and FMB samples; Bifidobacterium was related to FM formulations, while Alistipes was associated with FMPF and FMB, and Lactobacillus and Lacticaseibacillus were predominant in FMC. Trabulsiella was the central element in the FMC, while Mediterraneibacter was the central one in the FMPF and FMB networks. The FM formulations increased the acetic acid, and a remarkably high amount of propionic and butyric acids were detected in the FMB treatment. All FM formulations decreased the ammonium ions compared to the control; FMPF samples stood out for having lower amounts of ammonia. The probiotic FM with fruit pulp boosted the beneficial effects on the intestinal microbiota of healthy humans in addition to increasing SCFA in SHIME® and decreasing ammonium ions, which could be related to the presence of bioactive compounds.

Combining high intensity ultrasound and experimental design to improve carotenoid extraction efficiency from Buriti (Mauritia flexuosa)

Buriti (Mauritia flexuosa L.) is a significant source of carotenoids, but these compounds have been extracted using laborious and low-effective methods. The present work evaluated the high-intensity ultrasound combined with a chemometric approach to developing an optimal extraction method of carotenoids from buriti pulp. The multivariate optimization was carried out through two steps. First, a simplex-lattice mixture design was used to optimize the extractor solution finding higher extraction yield (903 ± 21 µg g−1) with the acetone:ethanol (75/25) mixture. After, sample mass (80 mg) and sonication time (30 min) were optimized applying central composite design (CCD) which provided a 14% improvement in the extraction method yield. So, the total carotenoid content (TCC) with optimal extraction conditions was 1026 ± 13 µg g−1which is almost twice the yield of methods known in the literature for buriti. The RP-HPLC-DAD analysis revealed that the carotenoids are gently extracted and β-carotene is the major compound in the extracts. To confirm the accuracy, buriti samples spiked with β-carotene standard and the developed method showed recovery >84% and precision <6.5%. Furthermore, the optimized ultrasound-assisted extraction (UAE) method was applied to other samples (tomato, guava, carrot, mango, acerola, papaya, and pumpkin) and presented a yield to 5.5-fold higher when compared to the reported methods indicating high robustness. Based on results, the UAE method developed has demonstrated feasibility and reliability for the study of carotenoids in buriti pulp as well as in other plant matrices with high biological relevance.

Characterization of Buriti (Mauritia flexuosa) Pulp Oil and the Effect of Its Supplementation in an In Vivo Experimental Model.

Mauritia flexuosa (Buriti) pulp oil contains bioactive substances and lipids that are protective against cardiovascular and inflammatory diseases. We performed physical and chemical analyses to verify its quality and stability. Buriti oil was stable according to the Rancimat test, presenting an induction period of 6.6 h. We evaluated the effect of supplementation with crude buriti oil and olive oil on metabolic parameters in 108 Swiss mice for 90 days. We investigated six groups: extra virgin olive oil (EVOO) 1 and 2 (1000 and 2000 mg/kg), buriti oil (BO) 1 and 2 (1000 and 2000 mg/kg), synergic (S) (BO1 + EVOO1), and control (water dose 1000 mg/kg). The animals were euthanized to examine their blood, livers, and fats. The supplementation did not interfere with food consumption, weight gain, and histological alterations in the liver. Group S showed the strongest relationship with the fractions HDL-c and non-HDL-c, indicating a possible cardioprotective effect. Moreover, we observed significantly higher IL-6 levels in the control, EVOO2, and BO1 groups than in the EVOO1 group. Resistin was also significantly higher for the synergic treatment than for the control. We conclude that BO combined with EVOO could be an excellent food supplement for human consumption.

Influence of buriti pulp (Mauritia Flexuosa L.) concentration on thermophysical properties and antioxidant capacity

This study aimed to determine the thermophysical properties and antioxidant capacity of buriti pulp with concentrations of 7, 9, 11, 13, and 15 °Brix. Total solids and moisture content were determined in an oven, and the specific mass was determined by the pycnometer method, while the specific heat, thermal diffusivity, and thermal conductivity were obtained using the KD2 Pro thermal properties analyzer. The antioxidant capacity was determined by the 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. At high concentrations, total solids and specific mass increased while moisture content decreased, and the soluble solids concentration did not influence specific heat, thermal diffusivity, and thermal conductivity. The antioxidant capacity of buriti pulps ranged from 0.07 to 10.45 μmol Trolox/g sample for the ABTS method and from 293.77 to 411.46 μmol Trolox/g sample for the DPPH method.

Lacticaseibacillus casei SJRP38 and buriti pulp increased bioactive compounds and probiotic potential of fermented milk

The effect of passion fruit and buriti pulps on probiotic and starter lactic acid bacteria growth and on fermented milk (FM) was evaluated. Buriti pulp showed higher amounts of lipids, total phenolic compounds (PC), β-carotene, lycopene and antioxidant activity (AA) than passion fruit. However, passion fruit was used more efficiently as a carbon source by all strains. Lacticaseibacillus casei SJRP38 showed the best results in the presence of passion fruit (1.8 log CFU/mL increase compared to the initial population) and the lowest reduction in the presence of buriti, thus it was selected to produce FM. Acidified milk (AM) with the same fruit pulps was used as a control. The buriti pulp did not affect the kinetic parameters of FM compared to the control. However, the addition of fruit pulps increased the PC in both FM and AM, and FM showed a greater increase (33.4–141.6%) than AM (21.4%–87.9%). Also, buriti pulp was able to increase (6–8%) the AA of the FM when compared to the AM. FM with buriti pulp stood out for its high amount of total PC, which indicates its high potential to be used as a functional food.

Choline chloride-based deep eutectic solvents do not improve the ethanolic extraction of carotenoids from buriti fruit (Mauritia flexuosa L.)

The aim of the present study was to evaluate the effect of choline chloride-based deep eutectic solvents (DESs) as co-solvents for the ethanolic extraction of total carotenoids from buriti fruit (Mauritia flexuosa L.). Choline chloride-based DESs were synthesized by the heating method. The systems were prepared using ethanol and DESs as a co-solvent in concentrations of 0.5–70.0% (w/v). In addition, a control condition was performed using ethanol as the sole solvent. The total carotenoid concentration in the systems was determined by spectrophotometry at 450 nm. The results showed that the ethanol used without co-solvent obtained total carotenoid yields of 10.06 ± 0.03 mg/g for the buriti pulp and 10.43 ± 1.27 mg/g for the buriti peel. In turn, the use of choline chloride-based DESs as co-solvents did not increase the ethanolic extraction yield of carotenoids. However, it was observed that the buriti peel can be a rich source of carotenoids with yields comparable to the fruit. Thus, it is suggested that new green solvents be evaluated to increase the recovery of total carotenoids from buriti fruit using ecofriendly processes.

Physicochemical characterization, bioactive compounds and antioxidant activity of pulp, peel, endocarp and food paste developed with buriti pulp and waste (&lt;i&gt;Mauritia flexuosa&lt;/i&gt; L.)

Buriti (Mauritia flexuosa L) is an Amazonian fruit with high potential to be used as a source of bioactive compounds and healthy food preparations due to its attractive yellow / orange color. The purpose of this paper was to verify the physicochemical characteristics, bioactive compounds and antioxidant activity in pulp, endocarp, peels, and three food paste developed with Buriti pulp and waste. In addition to the centesimal composition of the fractions of the fruit and the preparations, analyzes of vitamin C, phenolic compounds, carotenoids and antioxidant activity were also carried out. The following values were observed for the fractions of the fruit: vitamin C (48.44-55.22 mg/100 g), carotenoids (6.05-21.03 mg/100g), Total phenolics (19.31-33.30 mg GAEq/100g) and antioxidant activity (111.24-190.43 µmol TE/g DM), for all analyzed parameters, the highest values were observed for the peel. As for the formulations, it was found that the good attributes of the peel interfered in the formulation C, which presented the highest yields of vitamin C (21.22 mg/100g), carotenoids (13.99 mg/100g), Total phenolics (21.45 mg GAEq/100g) and antioxidant activity (82.34-130.52 µmol TE/g DM). Thus, it is verified that buriti has a great nutritional potential for direct consumption or to be used in the formulation of confectionery products, including the use of its peels in formulations.

Buriti pulp oil did not improve high-fat diet-induced metabolic disorders in c57bl/6 mice.

Metabolic syndrome (MetS) and obesity are growing in many parts of the world, becoming public health problems. It is proposed that foods with functional properties can assist in the treatment of these diseases. Crude buriti pulp oil (BPO) is a food traditionally consumed by residents in the Pantanal, Cerrado and Brazilian Amazon. It is rich in oleic acid, tocopherols and carotenoids, emerging as a potential functional food. Thus, this study aimed to evaluate the effect of the supplementation of BPO on metabolic disorders caused by a high-fat diet. Four groups of C57BL6 mice were used, a lean group with AIN-93M diet and control oil supplementation, an obese group with a high-fat diet and control oil supplementation, and two obese groups with a high-fat diet and BPO supplementation in the amounts of 50 and 100mg/kg. BPO worsened the metabolic state caused by the high-fat diet, worsening risk factors associated with MetS, as the abdominal circumference and retroperitoneal fat, serum levels of total cholesterol, uric acid, alanine transaminase, glucose and triglycerides, and renal fat, in addition to changes in glycaemic control and oxidative stress markers. C57BL/6 mice fed with a high-fat diet and supplemented with BPO presented a worsening in metabolic risk factors associated with MetS.

Buriti (Mauritia Flexuosa L.) pulp oil as an immunomodulator against enteropathogenic Escherichia coli

Bioactive compounds present in fruit oils have an antioxidant activity, which may be related to an immunomodulatory effect, which in turn can result in a microbicidal activity. Hence, the main aim of the current study is to evaluate the immunomodulatory effect of buriti oil (Mauritia Flexuosa L.) against enteropathogenic Escherichia coli (EPEC). Therefore, buriti pulp oil was extracted and characterized for saponification, peroxide and acidity index, total carotenoid content, fatty acid profile, hydrophilic and lipophilic fraction antioxidant capacity, cell viability, and phagocytosis of EPEC by mononuclear (MN) cells. The heat extraction process did not cause hydrolytic rancidity and oxidative rancidity in the oil, and the result is an acid value of 17.44 mg KOH/g of oil and a low peroxide value (0.062 meq peroxide/1000 g of oil). However, the saponification index was elevated (239.79 mg KOH/g of oil) due to the presence of low molecular weight fatty acid. As concerns the profile of the fatty acid, the oil is composed mainly of oleic acid (72.23 %) and palmitic acid (22.18 %). In addition, buriti oil presented a high content of carotenoids (760.5 ± 46.4 μg of β-carotene/g of oil), related to its antioxidant capacity. Moreover, buriti oil did not show toxicity to human blood MN phagocytes and increased the rate of cellular phagocytosis in EPEC and its microbicide index (69.3 ± 6.6 %), when compared with the negative control group. (48.1 ± 5.4 %).

Physicochemical composition and antioxidants of buriti (Mauritia flexuosa Linn. F.) – pulp and sweet

Buriti pulp has an elevated nutritional value and commercial potential, mainly because of its significant amounts of bioactive compounds. The objective of this research was to evaluate the physicochemical characteristics, the antioxidant capacity, and bioactive compounds of buriti pulps and sweets obtained from the Brazilian states of Goias, Tocantins and Para. The fruits obtained from Tocantins and Para presented greater lipid and energy contents. After processing, the sweets showed reductions in the protein, lipid, fiber and bioactive compound contents. The antioxidant activities and total phenolic compound contents were more significant in the pulp and sweets from Goias. There was a strong correlation between the phenolic compounds and the antioxidant activity. The total carotenoid content was greater in the buriti pulp and sweet from Tocantins but an 80% reduction in the bioactive compounds was observed in all the sweets produced. Thus, it was concluded that the origin of the fruit influenced its chemical composition and the antioxidant activity. However fruits and sweets can still be considered good dietary sources of bioactive compounds especially carotenoids.

Evaluation of the Antimicrobial Activity of Buriti (Mauritia Flexuosa) Pulp Extracts

Background: Buriti (Mauritia flexuosa) is a palm tree typical of the Amazon region. This plant belongs to the Arecaceae family and is economically important because it contains substances important for the food, cosmetic and pharmaceutical industries. It has, in its fruits, compounds with antimicrobial potential. Objective: The objective of this study was to evaluate the minimal inhibitory concentration and minimum bactericidal concentration of the Buriti extracts against the four bacteria: Salmonella enterica serotype Typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. Methods: 15 extracts from buriti pulp were obtained varying in temperature, mass of buriti and ethanol content. The antimicrobial activity of these extracts was evaluated. To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the methodology recommended by the Clinical and Laboratory Standards Institute was followed. Results: The results showed which buriti pulp extracts had strong inhibitory activity. Gram-positive results ranged from 21 to 78 μg.mL-1. For Gram-negative, they ranged from 30 to 111 μg.mL-1. Conclusion: The buriti extracts significantly slowed the growth of the tested bacteria.

Spray drying of the buriti pulp at different maltodextrin concentrations and temperature

In this work, the formulations prepared with buriti pulp and different maltodextrin concentrations (5, 10 and 15%) were dried in spray dryer and the chemical, physical and physical-chemical characteristics of the formulations and powder samples were evaluated. In this drying process two kinds of powder samples were collected: one into drying chamber and another in the cyclone. Using a factorial experimental planning, the experiments were conducted, in order to assess the moisture and ash contents, total acidity and density of both samples. The formulations dried in the spray dryer presented be feasible for the production of buriti pulp powder; however, powder samples collected into drying chamber and cyclone were different. Among the parameters analysed, it was found that only for density the samples collected into drying chamber, that the first-order empirical model obtained was statistically significant and predictive.

Buriti of the cerrado of Minas Gerais, Brazil: physical and chemical characterization and content of carotenoids and vitamins

Abstract The fruit species of the Brazilian Cerrado, such as buriti (Mauritia vinifera ), occupy a prominent position in the ecosystem since they contribute to the economic potential of the region and have high nutritional value. We aimed to evaluate the physical characteristics, chemical composition and content of vitamin C, carotenoids, vitamin E and folates in pulp of buriti from the Brazilian Cerrado of Minas Gerais, Brazil. The length, diameter, mass and fruit yield were evaluated. The titratable acidity was determined by volumetric neutralization; pH by potentiometry; soluble solids by refractometry; moisture using an oven; ash using a muffle furnace; proteins by the micro-Kjeldhal method; total dietary fiber by the gravimetric non-enzymatic method and lipids with a Soxhlet extractor. Vitamin C and carotenoids were analyzed by high performance liquid chromatography with detection by diode array (HPLC-DAD) and vitamin E and folates by HPLC with fluorescence detection. Buriti pulp showed 12.13 g.100g -1 of lipids and 74.86 g.100g-1 of moisture. Also, the fruit showed high content of dietary fiber, carotenoids, vitamin C, vitamin E and folates. Buriti can contribute to the adequate intake of fiber and vitamins and to preventing the incidence of disorders, especially those more frequent in native populations of the Cerrado.

Antioxidant potential of Buriti (Mauritia flexuosa) pulp flour in diabetic rats

Buriti pulp flour (BPF) contains significant levels of antioxidants. This study evaluated the effect of BPF on biomarkers of oxidative damage in the liver, heart, and pancreas of diabetic rats. The chemical composition, antioxidant capacity, and polyphenol content of BPF were determined. Thirty-six female Fisher rats were divided into four groups: control (C); control + BPF (CB); diabetic (D); diabetic + BPF (DB). Diabetes was induced by treatment with streptozotocin. Thirty days after the induction of diabetes, glucose, total cholesterol and triacylglycerides serum levels, aminotransferase and paraoxonase activities were evaluated. Oxidative damage to lipids and proteins was assessed through thiobarbituric acid reactive substances (TBARS) and protein carbonyl analyses, respectively. Histopathological analyses were also performed. BPF contained high concentrations of phenolic compounds, lipids, and fibers, and exhibited a high capacity to neutralize the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Diabetes was evidenced by equivalent high levels of glucose in plasma from rats in the D and DB groups. Diabetic rats in both groups also presented the same increased activity of aminotransferases. Protein carbonyl levels were increased in liver, heart, and pancreas in the D compared with C group. Although treatment with BPF did not result in any histopathological alterations, it reduced significantly the levels of TBARS in the heart and protein carbonyls in the liver and heart. No effect on blood glucose and tissue histology was observed following treatment with BPF. However, BPF diminished oxidative damage in liver and heart, indicating a possible antioxidant potential in vivo, in addition to in vitro.

ANTIOXIDANT ACTIVITY AND PHYSICOCHEMICAL CHARACTERISTICS OF BURITI PULP (Mauritia flexuosa) COLLECTED IN THE CITY OF DIAMANTINO – MTS1

ABSTRACT Buriti is a fruit with potential for economic exploitation due to its nutritional components; however, there are few studies about their post-harvest characteristics. Thus, the aim of this study was to determine the physicochemical characteristics and analyze the antioxidant activity of Buriti pulp. Fruits were harvested at full maturity stage, sanitized, pulped and stored under refrigeration until freezing. Then, pulp was submitted to the following analyses: moisture, protein, total carbohydrates and reducing carbohydrates, ashes, lipids, crude fiber, water activity, titratable acidity (TA), pH, color, ß-carotene, a-carotene, antioxidant activity and total energy value (TEV). Analyses were performed in triplicate, and the mean, standard deviation and variation coefficient were determined. Fruit pulp presented 0.98 of water activity, TA of 8.82g/100 g of citric acid, pH 3.78, 59.69% of moisture, 20.92% of fat, 8.56 % of crude fiber, 1.04% of ash, 7.28% of total carbohydrates, 4.50% of reducing carbohydrates, 9098µg/100g of ß carotene and 10086µg/100g of a-carotene. TEV found was 228.28 kcal/100g, the color analysis showed that the pulp has an average light tint value of L * = 59.69, with high hue H * = 68.36 and vivid color with C *= 62.03. Regarding the antioxidant activity, it was observed that Buriti is a fruit that can be used to combat oxidation, as it reduced by 82.42% the amount DPPH reagent used.

Comparison of the physicochemical profiles of buriti from the Brazilian Cerrado and the Amazon region

Demand for native fruits has been expanding, due to the increasing interest in foods with possible health benefits. The buriti (Mauritia flexuosa L. f.) is a native Brazilian fruit, appreciated by local populations, and its products are already on the market. This study investigated the physical characteristics and nutritional composition of buriti pulp, obtained from typical Cerrado and Amazon regions in Brazil. These regions influenced physical parameters and chemical composition. The fruits originating in the Cerrado were bigger and heavier than the ones from the Amazon. Nevertheless, protein, fatty acid and carbohydrate contents were higher in fruits from the Amazon region. The analyzed buriti fruits presented high monounsaturated fatty acid concentration (73.03 to 79.43 g/100 g), with the predominance of oleic acid (72.21 to 78.57 g/100 g). Buriti fruits may be a promising source of good nutritional quality vegetable oil and dietary fiber.

Elaboration of a cereal bar enhanced with flour of buriti pulp (Mauritia flexuosa L.)

The buriti (Mauritia flexuosa L) shows high percentage of sugar, proteins, and carotenoids among others. The developing of the cereal bar added with dehydrated fruit has become a very important activity due its popularity, practicality, nutritional value and sensory characteristics. This work had as a goal, elaborate a cereal bar enhanced with different concentrations of 10%, 20% and 30% buriti flour. About the buriti pulp physical and chemical composition, were found 60,33% of moisture, 3,69% of protein, 49,8% of lipids, 0,18% of ashes, 46,64% of carbohydrates and 4,7mg of carotenoid/100 g of buriti sample. About the cereal bar physical and chemical characterization, 12,4% of moisture, 11.96% of lipid percentage, 6,76% of protein percentage, 0,16% of ashes and 68,72% of carbohydrates percentage. As for the smell, the 30% flour sample revealed a significant difference from the 10% sample, but similarity with the 20% sample. About the buriti flavor, the 30% sample revealed a significant difference with the average of 8,1. This sample has shown bigger averages for the sensorial tonality, compression, cereal flavor, buriti flour smell and buriti flour flavor attributes, having a bigger rating of acceptability.

Characterization, thermal properties and phase transitions of amazonian vegetable oils

Thermal profiles of buriti pulp oil (Mauritia flexuosa Mart.), tucuma pulp and kernel oils (Astrocarium vulgare Mart.), rubber seed oil (Hevea brasiliensis), passion fruit oil (Passiflora edulis) and ucuuba butter (Virola surinamensis) were analyzed by thermogravimetry (TG/DTG) and differential scanning calorimetry (DSC). Gas chromatography and calculated iodine values were performed to determine the fatty acid profile and to measure the degree of unsaturation in these oils, respectively. The TG curves showed three steps of mass loss, which can be attributed to the degradation of polyunsaturated, monounsaturated and saturated fatty acids. The DSC crystallization and melting curves are reported and depended on the fatty acid composition. Usually, oil samples with a high degree of saturation showed crystallization and melting profiles at higher temperatures than the oils with a high degree of unsaturation. The data obtained by physicochemical analysis of oil samples were analyzed by principal component analysis and hierarchical cluster analysis to increase understanding of the data set, examining the presence or absence of natural groupings between samples.