BuOH Extract Research Articles

Comprehensive phytochemical analysis of various extracts of Kickxia lanigera growing in Turkey

In this study, the phytochemical contents of the extracts of Kickxia lanigera plant from Baskil district of Elazığ province in Turkey obtained in different organic solvents (hexane, chloroform, ethyl acetate and butanol) were determined by spectrometric methods such as GC-MS/FID, ESI-LC-MS/TOF and NMR. The result of GC-MS analysis: palmitic acid (19.08%), oleic acid ( 18.76%), dotriacontane (13.08%), CHCl3; linoleic acid (38.13%), oleic acid (34.08%), palmitic acid (13.79%) were determined in high amounts in hexane. HPLC-TOF/MS analysis showed that the CHCl3 and BuOH extracts had low levels of standard phenols, and the main components in the EA extract (in mg/kg plant) were cinnamic acid (5.92), hesperidin (4.7), apigetrin (4.5) and p-coumaric acid (2.8). the 1H NMR spectrum showed that the CHCl3 and EA extracts were rich in phenols.

Phenolic Constituents, Photoprotective Effect, and Antioxidant Capacities of Achillea ligustica All.

The present investigation was performed to figure out the chemical constituents and biological potential of polar extracts (AcOEt and BuOH) from Achillea ligustica, a medicinal species of the Asteraceae family. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was utilized to conduct a preliminary analysis of the phytochemical profiles of the AcOEt and BuOH extracts. The analysis revealed the existence of twenty compounds in the AcOEt extract and twenty-two in the BuOH extract, classified into various types of secondary metabolites. Subsequently, the exudate from the plant yielded five flavonoids, including two 6-methoxyflavonols identified for the first time in this genus. The isolation of compounds from AcOEt and BuOH extracts was achieved through the combined use of column chromatography (silica gel and Sephadex LH-20) and preparative TLC chromatography. The structures have been elucidated using 1D and 2D NMR spectroscopy, alongside comparisons with research data. Our study measured the total phenolic and flavonoid contents and carried out a comprehensive range of antioxidant tests using DPPH, GOR, CUPRAC, reducing power, and O-phenanthroline assays. Both extracts exhibited considerable antioxidant potential and contained high levels of phenolic and flavonoid compounds. The photoprotective effect of the AcOEt and BuOH extracts was evaluated in vitro by measuring the sun protection factor. Both extracts exhibited a high capacity for UV radiation absorption. Consequently, this plant presents an intriguing prospect for future research focused on incorporating it into photoprotective cosmetic products and pharmaceutical formulations.

Anticancer activity, phytochemical investigation and molecular docking insights of Citrullus colocynthis (L.) fruits

Cancer disease is regarded as one of the most significant public health issues, regardless of economic standards. Medicinal plants are now regarded as a natural source of anticancer medicines due to their antioxidant and anti-mutagenic actions. Cucurbitaceae is considered to be one of the most economically significant families. One family species is Citrullus colocynthis (L.), which has a high concentration of many active secondary chemical metabolites. Various C. colocynthis plant extracts showed cytotoxicity against some cancer cells. This study aims to identify the C. colocynthis fruit components and determine whether they have anticancer action against MIA PaCa-2 and A431 cells. High-Performance Liquid Chromatography/Quadrupole Time of Flight/Mass Spectrometry (HPLC/QTOF/MS); the technique was accustomed to investigate the compounds of the ethyl acetate (EtOAc) fruit extract. Anticancer activity was investigated on both MIAPaCa-2 and A-431 cell lines. DPPH assay for antioxidant activity was carried out. Molecular modelling was employed to help understand the molecular basis for the observed anticancer activity. 24 compounds were tentatively identified by comparing the extract’s fragmentation pattern in positive mode against reference compounds spectra and literature. The EtOAc extract of C. colocynthis had effective positive results on cancer cells (MIAPaCa-2 and A-431) and was characterized by slight or no harmful effect on normal (healthy) cells. For the DPPH assay, EtOAc and BuOH extracts exhibited high antioxidant activity (86 and 76%, respectively) compared with the oxidative potential of the standard compound (Caffeic acid, 98%). One of the major cucurbitacin derivatives that LC/MS tentatively identified in the EtOAc extract was Cucurbita-5(10),6,23-triene-3β,25-diol. During this study, docking experiments and MD simulations were carried out, which suggested the anti-pancreatic cancer activity of C. colocynthis extract to be attributed to EGFR inhibition by Cucurbita-5(10),6,23-triene-3β,25-diol. Therefore, expansion of this type of research should be encouraged in the hope of obtaining natural therapeutics for cancerous tumors in the future, having the advantage of being cheaper, safer, and with fewer side effects.

Antioxidant, Anti-Skin-Aging, Anti-Inflammatory, and Anti-Acetylcholinesterase Activities of Rourea oligophlebia Extracts.

The objective of this study was to evaluate the antioxidant, anti-skin-aging, anti-inflammatory, and anti-acetylcholinesterase activities of the hexane (n-hex), AcOEt, BuOH, MeOH, and aqueous extracts from R. oligophlebia roots. The total phenolic and flavonoid contents (TPC and TFC) were determined using Folin-Ciocalteu and AlCl3 colorimetric assays. The antioxidant capacity was examined by reducing power (RP), ferric reducing antioxidant power (FRAP), ABTS⋅+ , and DPPH⋅+ radical cation assays. All extracts potentially exhibited antioxidant activity with IC50 values ranging from 2.93 to 5.73 μg/mL for ABTS⋅+ and from 5.69 to 7.65 μg/mL for DPPH⋅+ except the n-hex extract. The BuOH, MeOH, and aqueous extract possess promising anti-skin-aging activities, as observed by an attenuation of UV-A toxicity on human keratinocytes. We proposed that these anti-skin-aging properties are possibly due to direct scavenging activity against reactive oxygen species and upregulate cellular antioxidant machinery. Moreover, we found that the antioxidant capacity was well correlated with anti-inflammatory capacity against nitric oxide (NO) production in terms of the n-hex, AcOEt, and BuOH extracts with IC50 values from 23.21 to 47.1 μg/mL. In contrast, these activities were found to be poorly correlated with AchE activity. To the best of our knowledge, this is the first report of the antioxidant, anti-skin-aging, anti-inflammatory, and anti-acetylcholinesterase activities of the extracts of R. oligophlebia roots. These findings indicated that this species could be a potential source of natural antioxidant, anti-aging, and anti-inflammatory agents. Consequently, it may be suggested as a medicinal plant that prevents diseases related to oxidative stress and inflammatory responses.

Antifungal Activities of Compounds Produced by Newly Isolated Acrocarpospora Strains.

In our continued search for bioactive metabolites from cultures of rare Actinobacteria resources from all over Taiwan and various natural ecological environments, an active antimicrobial strain of Acrocarpospora punica 04107M was collected in Taitung County in Taiwan and prepared from soil. The bioassay-guided fractionation of the BuOH extract of a culture broth from A. punica 04107M led to the isolation of five previously undescribed compounds: Acrocarposporins A-E (Compounds 1-5). All the constituents were confirmed by HRESIMS and 1D- and 2D-NMR spectroscopy. Their antifungal activity was also evaluated. Our results showed that four constituents (Compounds 1, 2, 4, and 5) possessed mild antifungal activity against Aspergillus niger, Penicillium italicum, Candida albicans, and Saccharomyces cerevisiae. It is worth mentioning that the chemical composition of Acrocarpospora punica 04107M has never been studied. This is the first report on diterpenoid metabolites from the genus Acrocarpospora.

Undescribed Metabolites from an Actinobacteria Acrocarpospora punica and Their Anti-Inflammatory Activity.

In an effort to explore bioactive anti-inflammatory compounds from natural Actinobacteria resources from all over Taiwan and various ecological environments, an active strain of Acrocarpospora punica was collected at Taitung County in Taiwan, prepared from soil origin. A bioassay-guided fractionation of the BuOH extract of a culture broth of a new strain of the actinomycete Acrocarpospora punica led to the isolation of five previously undescribed compounds: acrocarpunicains A-F (1-6). The structures were elucidated by 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry. Furthermore, the isolated compounds were subjected to in vitro testing to evaluate their anti-inflammatory activity. Of these isolates, acrocarpunicains A (1), B (2), C (3) and F (6) showed NO inhibitory activity with IC50 values of 9.36 ± 0.25, 10.11 ± 0.47, 5.15 ± 0.18, and 27.17 ± 1.87 μM, stronger than the positive control, quercetin (IC50 = 35.95 ± 2.34 μM). To the best of our knowledge, this is the first report on azaphilone and phenanthrene-type metabolites from the genus Acrocarpospora.

Comparison of Antioxidant activity Total Phenolic, and Total Flavonoids content of Butanolic extracts from Haloxylon scoparium and Traganum nudatum

The ability to protect the body from damages caused by free radical induced oxidative stress is an essential property of antioxidants, so they are an indispensable substance. A variety of free radical scavenging antioxidants are found in several dietary sources. The main objective of this study focused on the screening of antioxidant activity of two Saharian Chenopodiaceae: Haloxylon scoparium and Traganum nudatum. The in vitro antioxidant activity was investigated with DPPH radical scavenging assay and reducing power assay of the Butanolic extracts of these species. The Total Phenolic Content (TPC) was evaluated according to the Folin-Ciocaltue assay and Gallic acid was used as standard. The Flavonoids content (TFC) was evaluated by using the Aluminum Chloride method. The findings showed that the Bu-OH extract of Haloxylon scoparium exhibited better values of antioxidant activity, total phenolic content and total flavonoid content compared to those of Traganum nudatum.

Abstract 6261A: Chinese medicinal herbs Scutellaria barbata and Oldenlandia diffusa modulate apoptosis via induction of pro-apoptotic and anti-apoptotic proteins in MDA-MB-157 and 93B breast cancer cells

Abstract Scutellaria barbata (SB) and Oldenlandia diffusa (OD) have been used in Traditional Chinese Medicine for treating colon cancer, glioblastoma, and ovarian cancer liver, lung, and rectal cancers. These two herbs are included in most of the herbal cancer treatment formulas in China and Taiwan hospitals. We previously demonstrated that aqueous extracts of SB and OD inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo[a]pyrene in carcinogenesis. In studies by Hugo (2006) and Perez (2010), the efficacy of BZL 101 (an extract of SB) has been demonstrated in preliminary Phase Ia and Ib clinical trials in metastatic breast cancer patients. It is also shown to induce an S phase arrest of the cell cycle by affecting the Cycline A2 and CDK2 expression in estrogen-sensitive MCF7 and estrogen insensitive MDA-MB-231 breast cancer cells. MeOH and BuOH extract of OD have been illustrated to reduce MCF7 invasion via the inhibition of MMP-9 and ICAM-1 apoptotic proteins expression. Ursolic acid of OD could inhibit breast cancer metastasis by suppressing glycolytic metabolism through activating SP1/Caveolin-1 signaling. In this study, the effectiveness of aqueous extract of SB and OD in the modulation of apoptosis of breast cancer cell lines MDA-MB-157 and 93B (a drug-resistant APC-mutant of it) were investigated. Assessments were performed using green/red/blue fluorescent Apoptosis/Necrosis Detection Test and the Human Apoptosis Antibody Array - Membrane [43 Targets] Test (Abcam). Our fluorescent apoptosis data showed that 3-hours treatment with 2.5 mg aqueous extract of SB induced a statistically significant percentage of apoptosis in MDA-MB-157 (46.5 ± 7.5% > 16%± 1.0%, p < 0.05) and 93B (46.5 ± 7.5% > 16%± 1.0%, p < 0.05) as compared to the negative control. Similar results were obtained with the OD treatment. From the antibody array-membrane experiments, modulation of various apoptosis markers by SB such as the pro-apoptotic proteins BID and BIM (p=0.019; p=0.007) in MDA-MB-157 and Bad (p=0.002), Bax (p=0.020), BID (p=0.001), p27 (p=0.004), p53 (p=0.022) in 93B as compared to the negative controls; and anti-apoptotic markers BcL-2, FasL, IGF-I, and p21 were also observed. OD treatment also induced similar significant production of these two types of proteins. These results suggest that SB and OD contain phytochemicals that induce apoptosis in MDA-MB-157 and 93B via the modulation of these pro-apoptotic and anti-apoptotic proteins. Citation Format: MinSeo Kang, Nathaly Manrique, Rekha Issac, Sarah Wolf, Jasmine Cha, SungBeen Han, Hugo Jie Qin, Lun Kim, Padma P. Uppala, Brian Yuen Yau Wong. Chinese medicinal herbs Scutellaria barbata and Oldenlandia diffusa modulate apoptosis via induction of pro-apoptotic and anti-apoptotic proteins in MDA-MB-157 and 93B breast cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 6261A.

Phenolic constituents, antioxidant and α-amylase inhibitory activities of Pulicaria vulgaris growing in Tunisia: an in vitro and in silico study

The present research aimed to establish the polyphenolic profile and to appraise the antioxidant and anti-α-amylase activities of the EtOAc and BuOH extracts from the aerial parts of Pulicaria vulgaris. Total phenolics and flavonoids of both extracts were quantified. A total of 4 and 19 polyphenolic compounds were identified in the EtOAc (major component: methylcatechin: 261.51 ± 0.03 g kg−1) and BuOH extracts (major component: 1,3-O-caffeoylquinic acid:12.95 ± 0.12 g kg−1), respectively, by HPLC-PDA/ESI-MS analysis. P. vulgaris extracts showed interesting in vitro antioxidant properties. The EtOAc and BuOH extracts exhibited an α-amylase inhibitory action (IC50 values of 10.0 and 7.5 μgmL−1, respectively). It has been shown that the EtOAc extract produced an uncompetitive inhibition against α-amylase, while the BuOH extract inhibited this enzyme in mixed manner. The molecular docking analysis of the major constituents of BuOH extract showed a low binding energy, a correct mode of interaction in the active pocket of the enzyme, and an ability to interact with the key residues of glycosidic cleavage (GLU-230 and ASP-206), which can explain the strong inhibitory effect of α-amylase established by the BuOH extract. This is the first report on the polyphenolic composition, antioxidant and anti-α-amylase activities of the extracts from the aerial parts of P. vulgaris.

Evaluation of the EtOAc Extract of Lemongrass (Cymbopogon citratus) as a Potential Skincare Cosmetic Material for Acne Vulgaris.

This study evaluated the biological properties of lemongrass (Cymbopogon citratus) extracts. The EtOAc extract of lemongrass had DPPH, TEAC, and nitric oxide-scavenging activity assay results of 58.06, 44.14, and 41.08% at the concentration of 50, 10, and 50 μg/ml, respectively. The EtOAc extract had higher elastase and collagenase inhibitory activities than the 80% MeOH, n-hexane, BuOH, and water extracts and comparable whitening activity toward monophenolase or diphenolase. Also, the EtOAc fraction had higher lipase inhibitory and antimicrobial activities against Cutibacterium acnes among extracts which is known to an important contributor to the progression of inflammatory acne vulgaris, and an opportunistic pathogen present in human skin. Total phenolic and flavonoid concentrations in the EtOAc extract were 132.31 mg CAE/g extract and 104.50 mg NE/g extract, respectively. Biologically active compounds in lemongrass extracts were analyzed by LC-MS. This study confirms that lemongrass extracts have potential use as cosmetic skincare ingredients. Thus, lemongrass can be considered a promising natural source of readily available, low-cost extracts rich in antioxidant, skincare, and antimicrobial compounds that might be suitable for replacing synthetic compounds in the cosmeceutical industry.

A new sesquiterpene, prosoterpene, from Prosopis africana (Guill. & Perr.) Taub

A new sesquiterpene (Prosoterpene, 1) and eleven reported compounds (2–12) of several classes, such as flavonoids, alkaloids, phenolic acids, and long-chain alcohols, were isolated from the BuOH extract of Prosopis africana (Guill. & Perr.) Taub. Compounds 2–10 were reported for the first time from this plant. Isomers 11 and 12 were separated for the first time. Extensive spectroscopic techniques and literature comparisons were used to characterise their structures. Furthermore, compounds 3, 5–8, and 10–12 were performed for anti-glycation and cytotoxicity activities. Compound 3 (quercetin-3-O-α-L-rhamnoside) exhibited moderate anti-glycation activity. All tested compounds were non-cytotoxic against MCF-7 (breast cancer), NCI-H460 (lung cancer), Hela (cervical cancer), and BJ (normal human fibroblast) cell lines.

Chemical Composition, Anti-Quorum Sensing, Enzyme Inhibitory, and Antioxidant Properties of Phenolic Extracts of Clinopodium nepeta L. Kuntze.

Phenolic extracts of Clinopodium nepeta were prepared and their preliminary phenolic profiles determined using HPLC-DAD with 26 phenolic standards. Apigenin (21.75 ± 0.41 µg/g), myricetin (72.58 ± 0.57 µg/g), and rosmarinic acid (88.51 ± 0.55 µg/g) were the most abundant compounds in DCM (dichloromethane), AcOEt (ethyl acetate), and BuOH (butanol) extracts, respectively. The DCM and AcOEt extracts inhibited quorum-sensing mediated violacein production by C. violaceum CV12472. Anti-quorum-sensing zones on C. violaceum CV026 at MIC (minimal inhibitory concentration) were 10.3 ± 0.8 mm for DCM extract and 12.0 ± 0.5 mm for AcOEt extract. Extracts showed concentration-dependent inhibition of swarming motility on flagellated P. aeruginosa PA01 and at the highest test concentration of 100 μg/mL, AcOEt (35.42 ± 1.00%) extract displayed the best activity. FRAP assay indicated that the BuOH extract (A0.50 = 17.42 ± 0.25 µg/mL) was more active than standard α-tocopherol (A0.50 = 34.93 ± 2.38 µg/mL). BuOH extract was more active than other extracts except in the ABTS●+, where the DCM extract was most active. This antioxidant activity could be attributed to the phenolic compounds detected. C. nepeta extracts showed moderate inhibition on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, and α-amylase. The results indicate that C. nepeta is a potent source of natural antioxidants that could be used in managing microbial resistance and Alzheimer′s disease.

Evaluations of the curative efficacy of G. fruticosus solvent extracts in experimentally induced nephrolithiatic Wistar male rats

BackgroundIn Ethiopian folk medicine, there is a claim that medicinal plants can treat urolithiasis although there is insufficient scientific evidence. The objective of this study was to evaluate the curative efficacy of Gomphocarpus fruticosus extracts in experimentally induced nephrolithiatic rats.MethodsUrolithiasis was induced in male Wistar rats by feeding ethylene glycol in drinking water for 28 days. The curative effects were evaluated after oral administrations of 200 mg/kg of the extracts from 15 to 28 days. Urine samples were collected 1 day before sacrificing the rats. Blood, liver and kidney samples were gathered under anaesthetic condition at day 28. Crystals in the urine were also analyzed by light microscopy.ResultsG. fruticosus EtOAc extract reduced significantly the level of sodium (P < 0.001), whereas it was significantly elevated the levels of magnesium and citrate (P < 0.01) compared to lithiatic control. G. fruticosus BuOH extract lowered the levels of potassium (P < 0.01), calcium and phosphate in urolithiatic rats. It was also observed that G. fruticosus EtOAc extract decreased the level of oxalate in the urine (P < 0.001), whereas it was increased the levels of magnesium (P < 0.05) and citrate (P < 0.01) in serum analysis after exposure to BuOH extract. In the kidneys, CaOx crystal deposits were reduced significantly by G. fruticosus EtOAc extract (P < 0.01).ConclusionIt has been noted that G. fruticosus EtOAc extract was potent in treating urolithiasis. However, further study is required to assess the efficacy of the active compounds against urolithiasis.

Amazonian Siparuna extracts as potential anti-influenza agents: Metabolic fingerprinting

Ethnopharmacological relevanceSiparuna species are used in Brazilian Folk Medicine for the treatment and prophylaxis of colds, fever, headache, gastrointestinal disorders and rheumatic pain. Aim of the studyThis study aimed to investigate a possible anti-influenza activity of 25 extracts from leaves of Amazonian S. cristata, S. decipiens, S. glycycarpa, S. reginae and S. sarmentosa based on their folk medicinal uses as well as to investigate their metabolic fingerprinting. The chemical composition of the active extracts was further dereplicated. Material and methodsThe chemical composition of the crude EtOH extracts from five Siparuna species were investigated by ESI (±) LC-QTOF-MS2. Organic extracts were obtained by liquid-liquid partition with solvents of increasing polarity, generating 25 extracts which were subjected to a quick DI-ESI (±) IT-MS fingerprint analysis. These extracts were tested against influenza virus replication and cellular toxicity using MDCK cells and influenza A/Michigan/45/2015 (H1N1)pdm09 virus. The compounds in the active BuOH extracts from S. glycycarpa and S. sarmentosa were annotated by ESI (±) LC-QTOF-MS2. ResultsAnalysis of the EtOH extracts revealed the presence of alkaloids and flavonoids, in the positive and negative ionization modes. Out of the 25 organic extracts screened for their antiviral activity, the BuOH extracts from S. glycycarpa and S. sarmentosa were the most active, inhibiting 96.0 ± 1.3% and 89.5 ± 0.8% of influenza virus replication 24 h post-infection. These inhibitory effects were maintained until 72hpi. Alkaloids, O- and C-flavonoid glycosides, dihydrochalcones and a procyanidin dimer were annotated in these extracts. ConclusionsThe inhibitory effect against influenza A(H1N1)pdm09 virus replication shown by Amazonian Siparuna species corroborates the use of these plants in Brazilian Folk Medicine, showing their potential as anti-influenza agents. These promising results stimulate the continuation of this study with the aim of isolating the compound(s) responsible for this bioactivity, thus contributing to a better knowledge of those species and to the research of natural products with potential anti-influenza activity.

Antischistosomal and antioxidant protective role of Carica papaya fruit extracts against Schistosoma mansoni

Schistosomiasis is a major disease of public health in human. Patterns on immune response, worm recovery, in vitro antischistosomal bioassay screening (cercaricidal killing in vitro of Schistosoma mansoni was observed). In vivo Mice were infected by injection of 100 S. mansoni cercariae/mouse and treated by oral administration with crude extract, MeOH, ethyl acetate (EtOAc) and butanol (BuOH) Carica papaya fruits (4g/kg) start from 7th day p.i. to the end of experiment and a treatment control of 500 mg/kg of Praziquantel. Various concentrations of plant extracts were used in cercaricidal assay. Both crude extract and EtOAc showed significant dose-dependent percentage worm load reduction (P<0.001). Carica papaya, showed highly reduction in worm counts (60.3% and 68.2%) while Praziquantel (92.8%), elevated immune responses and least time in destroying cercariae. The highest significant reduction in dead ova (P<0.001) was observed in group treated with EtOAc extract. The level of IgG1 and IgG2 was significantly reduced (P<0.001) than in groups treated with C. papaya crude extract, EtOAc extract or PZQ as compared to levels in untreated infected mice. While the level of IgG1 and IgG2 was insignificantly reduced in groups treated with MeOH extract or BuOH extract as compared to levels in untreated infected mice. It was concluded from the results that the antischistosomal efficacy of the extracts was dependent being more potent in reducing both the worm burden and tissue egg load. The antishistosomal effect of C. papaya extracts was significantly higher in group treated with EtOAc extract. These findings supporting the potential use of C. papaya extracts in the management of schistosomiasis.

Comprehensive Phytochemical Characterization of Herbal Parts from Kidney Vetch (Anthyllis vulneraria L.) by LC/MSn and GC/MS.

Extracts of kidney vetch (Anthyllis vulneraria L.) are becoming increasingly interesting as ingredients for the health and cosmetics industry. However, comprehensive phytochemical investigations of this plant are scant in the literature. Thus, the aim of the present work was an in-depth characterization of semi-polar constituents from A. vulneraria. To capture a broad spectrum of compounds, the aerial parts of A. vulneraria were extracted with EtOH/water and the resulting crude extracts fractionated by partition between AcOEt and BuOH. Secondary plant metabolites were analyzed by HPLC-ESI-MSn and GC/MS. In a fraction obtained from the BuOH extract via Amberlite® XAD-7 purification glycosides of kaempferol, quercetin, isorhamnetin and rhamnocitrin were detected by LC/MSn , besides flavonoids acylated with meglutol (3-hydroxy-3-methylglutaric acid), acetic and ferulic acids. Moreover, aglycons were analyzed in extracts after 1 N HCl hydrolysis and derivatization with BSTFA. GC/MS analysis of the hydrolysates revealed the incidence of compounds like meglutol, OH/OMe-substituted benzoic acids, ferulic and fatty acids, flavonoids, sugars and the triterpenoid medicagenic acid. Furthermore, a hemolytic activity was detected in the AcOEt extract using a blood-agar assay, and this was ascribed to the occurrence of saponins. In a saponin fraction, obtained from the AcOEt extract by chromatographic purification, two main saponins were characterized by LC/MSn and HR-ESI-MSn . A pure sapogenin could be isolated via VLC and CC purification upon acid hydrolysis of the saponins and assigned to saikogenin D by NMR analysis.

Characterization of Secondary Metabolites in Flowers of Sanguisorba officinalis L. by HPLC-DAD-MSn and GC/MS.

The investigations reported here focus on an in-depth characterization of the secondary metabolite profile of Sanguisorba officinalis flowers. For this purpose, fresh flowers were extracted with MeOH/H2 O and EtOH/H2 O and the resulting crude extracts fractionated using CH2 Cl2 , AcOEt, and BuOH. Individual compounds were characterized by high performance liquid chromatography and gas chromatography coupled with mass spectrometric detection (HPLC-DAD-MSn and GC/MS). MeOH/H2 O extraction and LC/MSn investigations revealed the occurrence of flavonoid glycosides (quercetin, kaempferol), ellagitannin glycosides and four anthocyanins. Among the latter, two components, i. e., cyanidin-malonyl-glucose and cyanidin-galloyl-hexose, have not been reported for S. officinalis so far. Furthermore, phenylethylamine was characterized for the first time in Sanguisorba by pH value dependent extraction with CH2 Cl2 . In addition, AcOEt and BuOH extracts were analyzed by GC/MS both prior to and after acid hydrolysis of secondary metabolites. For this purpose, the extracts were treated with 1 n HCl solution (105 °C, 1 h) and derivatized with BSTFA. Analyses revealed the occurrence of several classes of phenolic compounds, such as gallic acid, hydroxybenzoic acid, hydroxycinnamic acid and ellagic acid derivatives. Additionally, the most prominent ursane-type triterpenoid (ziyu-glycoside I) from Sanguisorba and its corresponding aglycone isomers were detected and assigned based on their characteristic fragmentation patterns.

Characterization of Polar and Non-Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia.

This work investigated the polar (PC: protein, amino acid and metabolite) and non-polar (NPC: fatty acid) compounds and bioactivity characteristics of the EBN harvested from the state of Johor in Malaysia. The electrophoretic gels exhibited 15 protein bands (16-173 kD) with unique protein profile. Amino acids analysis by AccQ⋅Tag method revealed 18 types of amino acids in EBN. Metabolite profiling was performed using High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometer (HPLC-QTOF/MS) technique and a total of 54 compounds belonging to different groups were detected and identified. These findings help to uncover the relation of therapeutic activity of EBN. The EBN was further extracted with AcOEt and BuOH. The AcOEt extract was fractionated into three fractions (F1 -F3 ), and the high triglyceride content in F2 was verified by gC-FID. The three groups of fatty acids discovered in EBN are 48.43 % of poly-unsaturated (PUFA), 25.35 % of saturated fatty acids (SFA) and 24.74 % of mono-unsaturated fat (MUFA). This is the first time to report results ofEBN, BuOH, and AcOEt extracts and of fraction F2 (TEBN) on their analysis for their antioxidant activities by DPPH, ABTS and catalase assay and for their paraoxonase and anti-tyrosinase activities. The results showed that TEBN exhibited the significant bioactivity in all assays. These findings suggest that TEBN is a good source for natural bioactive compounds in promoting body vigor. Current work widened the content of EBN especially on the triglyceride and also marked the content of specific location (Johor, Malaysia) of EBN origin.

PRELIMINARY PHYTOCHEMICAL SCREENING, GC-MS PROFILING AND IN VITRO EVALUATION OF BIOLOGICAL ACTIVITIES OF GARCINIA ATROVIRIDIS ROOT EXTRACTS.

01Jan 2019 PRELIMINARY PHYTOCHEMICAL SCREENING, GC-MS PROFILING AND IN VITRO EVALUATION OF BIOLOGICAL ACTIVITIES OF GARCINIA ATROVIRIDIS ROOT EXTRACTS Nur Salsabila Ahmad Roslan , Seema Zareen , Normaiza Zamri and Muhammad Nadeem Akhtar. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Malaysia.

IN VITRO ANTIOXYDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF EXTRACTS FROM THE ENDEMIC ARGANIA SPINOSA (L.) SKEELS FROM ALGERIAN SAHARA

The BuOH fractions prepared from 80% MeOH extract of the leaves from the endemic Saharan tree Argania spinosa (L.) Skeels exhibited the most potent antioxidant capacity ( IC50 = 2.41 ± 0.38 mg/mL for DPPH and 7.95 ± 0.54 mg/mL for superoxide anion radical), compared to the EtOAc and CHCl3 fractions. The results showed that BuOH extract fraction exhibited a strong antioxidant activity and had the most potent scavenging abilities which may be correlated to the presence of Phenolic compounds.