Bunch Of Grapes Research Articles

GROSS MORPHOLOGY AND MORPHOMETRICAL STUDY OF FEMALE REPRODUCTIVE TRACT IN SIRUVIDAI CHICKEN DURING LAYING AND BROODINESS PHASE

Avian species reproduce sexually by the fusion of male and female gamete spermatogonia and oogonia-produced by differentiated testes and ovaries. Fertilization is internal and all birds are oviparous. Reproduction in birds entirely varies from other animals. The two essential reproductive organs ere the ovary and oviduct which were involved in the synthesis of egg in adult laying hen. Gross and morphometrical studies were carried out on the female reproductive tract in twelve Siruvidai chicken of which six at laying phase and six at broodiness phase. The birds were reared in Poultry Research Station, Madhavaram, Chennai-51. In laying phase, the left ovary showed mature ovarian follicles whereas the ovary during broodiness phase was in the form of a bunch of grapes with no hierarchical follicles. There was no significant difference in the body weight of the bird during laying and broodiness. The mean length, weight and width of ovary and oviduct had a highly significant difference during laying and broodiness phase. Gross morphology revealed that only the left female reproductive tract was well-developed both during laying and broodiness phase and the morphometric data analyzed will provide a baseline for subsequent studies and also for comparison with other avian species.

Twist and turn of an unusual entity: A rare case report of torsion of huge epididymal cyst from a tertiary hospital

An epididymal cyst (EC) is a benign lesion. It arises due to cystic degeneration of the epididymis. The torsion of it is extremely rare. The usual presentation of the cyst is asymptomatic scrotal swelling. The swelling has a lobulated surface and feels like a bunch of grapes. The huge EC is rare and moreover, it is associated with torsion-rarest presentation.

Comparative Study of the Effect of Fertigation and Drip Irrigation Technologies on the Development of Grapevines (In Laboratory Conditions)

The article provides information on determining the loss during evaporation and water conservation of irrigation water supplied to grape plants in vegetative vessels studied in laboratory conditions using injection-ferritization technology, on the study of mineral fertilizers dissolved in water and applied under the plant, and on the growth of grape plants. It is shown that the vegetation experiments were carried out in two variants: fertigation technology and drip irrigation technology. As a result, it was found that the water level of the injector of grape plants in the growing vessels is 0.5 l/h, the duration of watering during the growing season was 264 hours, with 11 watering 132 liters of water were given. When using drip irrigation technology, the water consumption by a dropper is 0.5 l/h, the duration of irrigation is 336 hours, 168 liters of water were used with 14 times watering, which means saving irrigation water by 27% with ferritization. When using fertigation technology, it was found that inorganic fertilizers dissolve in water 10 times faster with forced mixing than in the absence of forced mixing. This makes it possible to apply fertilizers with water in less time and save its norms. Also, in the vegetative vessel with fertigation technology, the mother bunch of grapes in the budding phase was on average 14 cm larger than with drip irrigation technology.

ПОДВОИ И НОРМА НАГРУЗКИ ДЛЯ ВИНОГРАДА ЛИВИЯ

In the course of the experiment, the differentiat-ed influence of various rootstocks and the norms of the bush load with shoots on the agrobiologi-cal indicators of grapes was established. The ob-ject of research was the grapevine variety Livia, rootstocks SO4 and 41B. The studies were car-ried out in the conditions of a moderate conti-nental climate in the Central agroecological zone of viticulture of the Krasnodar region, on irrigat-ed vineyards in a covering culture. Scheme of planting bushes was 3.8×2 m. The soils are low-humus, leached powerful chernozems. The most productive were the vineyards on the rootstock 41B. The bunch size on the 41B rootstock was 1.34 times greater than on SO4, the yield capaci-ty – 1.56 and amounted to 696 g and 28.23 t/ha, respectively. The predominant influence of the 41B rootstock in comparison with SO4 on the productivity of the Livia grapes is preserved with a differentiated load of grape bushes with bunches. With a maximum load of bushes with bunches (SO4 – 33, 41B – 38 pcs./bush), their weight on the 41B rootstock was 43 % higher than on SO4, yield capacity – by 62 %, with an average load (SO4 – 27, 41B − 32 pcs./bush) – by 27 and 48 %, respectively, at minimum load (SO4 – 19, 41B – 23 pcs./bush) – by 32 and 59 %, respectively. The influence of rootstocks on the norm of bush load by shoots is not unam-biguous. Against the background of the maxi-mum number of bunches on bushes, the highest values of bunch weight and grape yield capacity on SO4 and 41B were at loads of 25 and 26 shoots/bush, average – 25 and 33 shoots/bush, minimum – 31 and 20 shoots/bush, respectively. For growing high yields and attractive sizes of bunches of table grapes of Livia variety in the Central agroecological zone of the Krasnodar re-gion, it is recommended to use rootstock 41B, the load norm of bushes with shoots of 26 and bunches of 38 pcs./ bush.

Magnetic resonance imaging and next-generation sequencing for the diagnosis of cystic echinococcosis in the intradural spine: a case report

BackgroundCystic echinococcosis (CE) is a parasitic zoonotic disease caused by the larval stage of Echinococcus granulosus. The liver and lungs are the most common sites for infection. Infection of the intradural spine is rare.Case presentationA 45-year-old woman of Han ethnicity presented with a chronic history of recurrent lumbar pain. Magnetic resonance imaging of the lumbar spine revealed the classical characteristic of multiple cystic lesions of variable sizes, manifesting a “bunch of grapes” appearance, localized within the spinal canal at the L4–L5 vertebral level. In the meanwhile, metagenomic next-generation sequencing identified Echinococcosis granulosa. The patient underwent surgery to remove the cyst entirely and subsequently took albendazole 400 mg orally twice daily for 6 months.ConclusionSpinal CE should be suspected in patients with multiple spinal cystic lesions and zoonotic exposure. metagenomic next-generation sequencing serves as a robust diagnostic tool for atypical pathogens, particularly when conventional tests are inconclusive. Prompt and aggressive treatment for spinal cystic echinococcosis is imperative, and further research is warranted for improved diagnostic and therapeutic strategies.

“Bunch of Grapes”: A Case of Innumerable Endometriotic Cysts

“Bunch of Grapes”: A Case of Innumerable Endometriotic Cysts

Impact of Smoke from Wheat, Oat, and Clover Stubble Burning on Cabernet Sauvignon Grapes and Wine

Background and Aims. Stubble burning is an agricultural practice employed by some grain growers to prepare farmland for sowing and/or to control weeds and pests. Grapegrowers and winemakers have questioned whether the resulting smoke can contaminate grapes in nearby vineyards. This study therefore sought to determine the potential for smoke from three different stubble burns to taint grapes and wine. Methods and Results. Excised bunches of mature Cabernet Sauvignon grapes were exposed to smoke from prescribed burning of wheat, oat, and Balansa clover stubble windrows. Environmental sensors monitored the concentration of particulate matter as a measure of smoke density, while chemical and sensory analysis established the extent to which grapes and wine were tainted by smoke. Only grapes that were positioned among the burning wheat windrows or downwind, but in close proximity (∼200 m) to the oat stubble burn, were exposed to sufficient quantities of smoke to result in a detectable concentration of volatile phenols (up to 12 µg/kg), as chemical markers of smoke taint. These grapes yielded wines with two to threefold higher volatile phenol concentrations (up to 18 µg/L) than other wines, including the control wine, and low intensity, but perceptible smoke-related sensory attributes, indicative of low-level smoke taint. Conclusions. Chemical and sensory analyses suggest the risk of smoke taint from stubble burning is low, except where vineyards are immediately downwind and/or prolonged or repeated smoke exposure occurs. However, stubble moisture content and prevailing weather conditions affect smoke density and dispersion, and will therefore affect the potential for smoke contamination by grapes. Significance of the Study. This study will assist colocated grain growers and grape and wine producers to undertake commercial operations, without negatively impacting one another.

The magnetic resonance characteristics of sinonasal rhabdomyosarcoma in adults: analysis of 27 cases and comparison with pathological subtypes

BackgroundSinonasal rhabdomyosarcoma (RMS) in adults is extremely rare, and early diagnosis and treatment are crucial to improve the patient’s prognosis. The purpose of this study was to evaluate the magnetic resonance imaging (MRI) findings of sinonasal RMS in adults and analyze the correlations between the imaging features and pathological subtypes.MethodsWe reviewed 27 patients with pathologically proven RMS of the nasal cavity and paranasal sinuses, including embryonal RMS (ERMS) in 14 patients, alveolar RMS (ARMS) in seven patients, and mixed-type RMS in six patients. Conventional MRI was performed in all 27 patients, and high-resolution diffusion-weighted imaging was conducted in 25 patients. The tumor location, size, morphological features, signal intensity, texture, contrast enhancement characteristics, lymph node metastases, apparent diffusion coefficients (ADCs), and involvement of local soft tissues were independently assessed by two authors.ResultsOn MR imaging, sinonasal RMS appeared isointense on T1-weighted imaging in 21 cases (77.8%) and heterogeneously hyperintense on T2-weighted imaging in 18 patients (66.7%). After enhancement, the tumors were heterogeneously enhanced in 24 cases (88.9%). Botryoid enhancement with multiple small rings resembling bunches of grapes was found in 15 cases (55.6%). Mucosal invasion of the maxillary sinus was identified in 51.9% patients. Skull and orbit involvement were found in 55.6% and 81.5% patients, respectively. Lymph node metastasis was seen in 18 cases (66.7%). There were significant differences in botryoid enhancement (P = 0.044) and skull involvement (P = 0.044) among different histological subtypes. The mean ADC value of RMS was 0.73 ± 0.082 × 10–3 mm2/s, and there was no significant difference among different histological subtypes.ConclusionsSome characteristic MRI findings such as botryoid enhancement in the ethmoid sinus, involvement of the orbit and skull, and a lower ADC value can provide important clues for preoperative diagnosis of sinonasal RMS in adults. Further, botryoid enhancement was more common in ERMS, while skull involvement was more common in ARMS.

Rapid In-Field Volatile Sampling for Detection of Botrytis cinerea Infection in Wine Grapes.

Fungal infection of grape berries (Vitis vinifera) by Botrytis cinerea frequently coincides with harvest, impacting both the yield and quality of grape and wine products. A rapid and non-destructive method for identifying B. cinerea infection in grapes at an early stage prior to harvest is critical to manage loss. In this study, zeolitic imidazolate framework-8 (ZIF-8) crystal was applied as an absorbent material for volatile extraction from B. cinerea infected and healthy grapes in a vineyard, followed by thermal desorption gas chromatography-mass spectrometry. The performance of ZIF-8 in regard to absorbing and trapping the targeted volatiles was evaluated with a standard solution of compounds and with a whole bunch of grapes enclosed in a glass container to maintain standard sampling conditions. The results from the sampling methods were then correlated to B. cinerea infection in grapes, as measured and determined by genus-specific antigen quantification. Trace levels of targeted compounds reported as markers of grape B. cinerea infection were successfully detected with in-field sampling. The peak area counts for volatiles 3-octanone, 1-octen-3-one, 3-octanol, and 1-octen-3-ol extracted using ZIF-8 were significantly higher than values achieved using Tenax®-TA from field testing and demonstrated good correlation with B. cinerea infection severities determined by B. cinerea antigen detection.

Investigation of the influence of fermentation conditions on the content of biologically active substances of the phenolic complex in young red wines

The article presents data on the study of the influence of fermentation conditions on the content of biologically active substances of the phenolic complex in young red wines.Red wines, due to the high content of phenolic substances in them, have P-vitamin activity, antiglycemic, antihepatoxic and antimutagenic effects, as well as antimicrobial and other types of biological activity. Many of the phenolic substances act as natural antioxidants, have a radical-cleansing ability, and help reduce blood cholesterol levels. Thanks to these properties, red wines contribute to the prevention of many diseases and correct the antioxidant status of a person.The objects of the research are wine materials obtained from grapes of the Moldova variety by the method of carbon dioxide maceration, with a process duration of 4 days or 7 days. In the second case, additional introduction of carbon dioxide of exogenous origin hasa been used. As a control one, wine material obtained by traditional technology has been used, as a result of must fermentation on pulp with a floating cap.The influence of fermentation conditions on the total content of monomeric and polymeric phenolic compounds, as well as on the mass concentration of individual phenolic acids and flavonoids, has been studied.It has been established that an increase in the duration of maceration of a bunch of grapes up to 7 days, as well as the additional introduction of CO2 of exogenous origin, have contributed to the intensification of extraction processes and led to a natural increase in the concentration of substances of the phenolic complex.Thus, the possibility of using carbon dioxide maceration, which ensures the optimal accumulation of substances of the phenolic complex, has been established for the production of young red table wines, especially when processing high-acid grape varieties with a high content of anthocyanins and tannin. Carrying out carbon dioxide maceration of whole bunches of grapes under conditions of anaerobiosis has contributed to the preservation of biologically active substances in wine, including vitamins, stilbenes, phenolcarboxylic acids.

Effects of Different Freezing Treatments during the Winemaking of a Varietal White Wine with Regard to Its Phenolic Components.

In white wine production, the technique consisting of freezing whole or crushed grapes usually increases the levels of aroma-related compounds in the final wine products. However, this technique may affect phenolic compounds, among other chemical compounds. Phenolic compounds are crucial to white wines because of their susceptibility to oxidation and their role with regard to color stability. In this study, white wines made from Muscat of Alexandria grapes were subjected to two different freezing techniques: whole-bunch freezing and crushed-grape freezing. In addition, a pre-fermentative maceration was applied to each experiment in order to determine if the effects of freezing were comparable to those of maceration. The phenolic compounds studied were gallic acid, protocatechuic acid, caffeic acid, trans-coutaric acid, and epicatechin, which are the key compounds from the point of view of wine stability. The freezing of crushed grapes enhanced the extraction of phenolic compounds in comparison to the freezing of whole bunches of grapes without pre-fermentative maceration. On the other hand, the effect of pre-fermentative maceration was comparable to that resulting from freezing crushed grapes. This step made the must from whole frozen grapes having even larger levels of phenolic compounds. Without pre-fermentative maceration, freezing whole bunches of grapes only allowed a moderate extraction of phenolic compounds and produced wines with lower individual phenolic contents than those obtained through traditional winemaking procedures.

‘Bunch of Grapes’ at the Occipital Lobe with 3.8ppm Singlet Peak on MRS: A Case of Tuberculoma

‘Bunch of Grapes’ at the Occipital Lobe with 3.8ppm Singlet Peak on MRS: A Case of Tuberculoma

A Case of Haemorrhagic Herpes Zoster

Background: Varicella-zoster virus (HHV3) is one of the virus in herpes family. Herpes zoster results from the reactivation of varicella-zoster virus in the dorsal root ganglion. This reactivation occurs in immunocompromised conditions such as people with cancer, organ transplant recipients or those receiving chemotherapy, and people with HIV. Old age is also an important risk for the development of herpes zoster. Immunocompromised patients are at increased risk of VZV reactivation because of reduced T cell-mediated immunity. Emotional stress has also been mentioned as an associated factor in people with herpes zoster. The Classic clinical presentation consists of clear vesicular eruptions in dermatomal distribution which are painful. An immunocompromised individual may have frequent attacks of herpes zoster, cutaneous dissemination, organ involvement, and hemorrhagic herpes zoster. Haemorrhagic herpes zoster is an atypical form of herpes zoster develops in patients who are immunosuppressed and people of advanced age who are taking antiplatelets, and anticoagulants, and also in patients with thrombocytopenia due to any cause. The most common Association of herpes zoster is observed with lymphocytic leukemia and less frequently with myeloid leukemia. Management icludes antiviral therapy along with management of haemorrhagic conditions. Case Report: A nondiabetic male of 60 years attended the medicine outpatients department with complaints of epistaxis for two days. No history of bleeding from other sites was evident. There was no history of trauma or taking of anticoagulants or antiplatelets. No history of fever. The patient was anaemic and an erythematous rash was evident along the right T6 dermatome. He was admitted to the general medicine ward. On the second day of his admission, our patient developed painful haemorrhagic bullous lesions over the right T6 dermatome resembling a bunch of grapes and erythematous popular lesions all over his body (Figure 1). The bullous lesions became larger in size in the subsequent two days of admission. On query, he mentioned that he had suffered from Varicella Zoster in his early childhood. Patients haemoglobin was 10.70 gm/dl, total count of white blood cell (WBC) was 13.20 K/ mm3 (normal value 4.00 – 11.00 K/ mm3), neutrophil and lymphocyte counts were 2.38 K/ mm3 (normal value 2.16 – 6.04 K/ mm3) and 10.38 K/ mm3 (normal value 0.6 – 3.06 K/ mm3) respectively. His platelet count was 5.00 K/ mm3 (normal value 150.00 – 400.00 K/ mm3). The patient denied skin biopsy. A diagnosis of haemorrhagic herpes zoster was made after a dermatology consultation and oral valacyclovir was started along with oral and topical antibiotics and regular dressings. After six units of platelet transfusion patients, the platelet count was raised to 30 K/ mm3. Peripheral blood film was suggestive of acute leukaemia. A bone marrow examination was suggestive of acute myeloid leukaemia. The patient was transferred to the haemato-oncology ward for further planning and management. Conclusion: Haemorrhagic herpes zoster may occur in different clinical conditions and one of them although rare, is acute myeloid leukaemia. Clinical scenario along with bone marrow and blood picture helps to reach a diagnosis in such cases.

Yuan Longping, a great world hunger fighter

Yuan Longping, a great world hunger fighter

Bunch of grapes – I saw the sign

As early as the late 1800s, the appearance of hydatidiform moles have been likened to grapes. The bunch of grapes sign is an ultrasound finding of a heterogenous mass within the uterine cavity with multiple anechoic spaces. We saw the bunch of grapes sign on a 28-year-old on ultrasound.

Monitoring of disease and pests in vineyards and their levels of harmfulness

This article monitors the spread and damage of oidium (Uncinula necator Burrill), anthracnose (Gloeosporium ampelophagum Sacc) and mildew (Plasmopara viticola Berl & De Toni) diseases in the vineyards of Tashkent, Samarkand and Fergana regions of Uzbekistan. In addition, additional monitoring work was carried out on the spread of the external quarantine vine bacteriosis Pierce's disease (Xylella fastidiosa Wells et al) in the vineyards of Uzbekistan. According to the results of research and observation in 2022, vine oidium disease is relatively low in the vineyards of Samarkand and Fergana regions (from 5.0% to 30.0% in leaves, branches and grape bunches), and strongly in the vineyards of Tashkent region (12% in leaves, branches and grape bunches). .0% to 100.0%) have been found to be harmful. Anthracnose disease is widespread in the vineyards of Tashkent and Samarkand regions (from 10.0% to 75.0% on leaves, branches and bunches of grapes), causing severe damage to vineyards, and it is relatively rare in Fergana region (from 5.0% to 20.0% on leaves, branches and bunches of grapes) was found to be spread.

A Shine Muscat Grape Berry Detection and Grape Cluster Compactness Estimation for Assessment of Grape Quality Based on Instance Segmentation Methods

Highlights Mask R-CNN ResNet-101 was analyzed to have the highest detection accuracy for grape berries. Grape cluster compactness is one of the important factors that determines the quality of grapes. Indicator DGC was used to intuitively calculate the compactness of grape cluster. An average error of 1.6 mm occurred with the verification of diameter estimation algorithm. Abstract. Consumption and cultivation of grapes are constantly increasing because they are known as alkaline foods, which relieve fatigue by accelerating carbohydrate metabolism in the human body. Among these grapes, a Shine Muscat doesn’t have the bitter taste of ordinary grapes, has low acidity, and has a crunchy texture, which results in growing interest in modern society. As the consumption of Shine Muscat increases, an automated process to save labor and time becomes essential. Particularly, one challenge that needs to be solved is the determination of grape quality based solely on visual evaluations by experts, which can result in inconsistent pricing for consumers. In this study, an algorithm was adopted to detect the grape berries from a bunch of single grapes by acquiring RGB images of the harvested Shine Muscat. Mask R-CNN, a convolutional neural network-based image segmentation technique, was used with various backbones to compare and evaluate the performance of each model. Results showed that Mask R-CNN ResNet 101 had the highest AP (Average Precision) value of 0.961 among all models. In addition, indexes such as the size (diameter) of each grape berry, the area of the grape on the image, and the area of the empty space that are required to find the compactness of the grape cluster are obtained. In particular, it was analyzed that the average error value (mm) and percent error (%) of the diameter estimation algorithm developed in this study were 1.60 mm and 4.79%, respectively. Keywords: Keywords., Density of Grape Cluster (DGC), Diameter estimation, Grape berry, Grape cluster compactness, Mask R-CNN, Object detection.

First report of Lasiodiplodia theobromae associated with panicle blight of grapes (Vitis vinifera L.) in India.

Grape (Vitis vinefera L.) is a popular horticulture crop in Karnataka, India. A fungal pathogen caused panicle blight on panicles with immature fruit and severity increased subsequently in the grape growing regions of Devanahalli and Doddaballapur, Karnataka, between August and September 2019. The disease incidence varied from 15 to 18 percent in around 45 hectares of grape vineyards surveyed. The associated fungal pathogen was isolated on Potato Dextrose Agar (PDA) medium (HiMedia Laboratory, Mumbai, India) amended with Chloramphenicol. A total of 12 fungal isolates were obtained and identified based on morphology. Fungal cultures obtained from all the panicle blight affected samples were fluffy grayish to black with profuse, dense mycelium. Microscopic examinations revealed that the conidia ellipsoidal, two celled and hyaline when young, and developed dark brown pigments at maturity. Mature conidia measured 18.24±2.35 to 26.62±3.11 μm long and 10.32±1.08 to 12.57±1.82 μm width (n=30). The fungal pathogen was identified as a Lasiodiplodia sp. based on colony morphology and microscopic features. A total of three representative isolates L. theobromae (Vv12, Vv15, and Vv19) were selected for molecular identification based on ITS-rDNA, tub2 and EF-1α gene sequences and phylogenetic analysis. Genomic DNA was isolated from 12 day old cultures and ITS-rDNA, tub2 and EF-1α genes were amplified using ITS1/ITS4; Bt2a/Bt2b and EF1-728F/986R primer pairs, respectively (White et al., 1990; Glass and Donaldson, 1995, Carbone and Kohn, 1999). PCR amplicons were sequenced and the sequences were deposited in GenBank with the accession number ITS: MZ855866.1; MZ855867.1; MZ855868.1; tub2: MZ868708.1; MZ868709.1; MZ868710.1 and EF-1α: OM604750; OM604751; OM604752 respectively. The phylogeny was constructed based on combined ITS, EF-1α and the tub2 regions. Maximum Likelihood (ML) analysis was conducted and an ML tree was constructed with the substitution models (branch support was evaluated by 1,000 bootstrap replications). Combined phylogeny confirmed that the sequences shared a common clade with L. theobromae. Based on micro-morphological features and multi-locus sequence phylogeny, the associated fungal pathogen was identified as L. theobromae. There are no reports on the occurrence of L. theobromae causing panicle blight on grapes from India. Further, the pathogens association was confirmed through pathogenicity assay conducted on field harvested healthy bunches of grapes maintained under humid chamber. A total of 10 grape bunches were inoculated with a mycelial disc on the rachis of the panicle and incubated in a moist chamber for 5 days and control sets were inoculated with only agar plugs. The experiments were conducted in three replicates and repeated twice. A total of 21 panicle bunches developed typical rot symptoms 12-days post inoculation. The identity of the pathogen was confirmed based on micromorphology and cultural features after re-isolation (n=5), thus proving the Koch postulates and confirming the association of L. theobromae with panicle blight of grapes. Lasiodiplodia species are known to cause dieback, stem blight, leaf blights and spots on various crop plants. Mathur (1979) mentioned the occurrence of L. theobromae on grapes, however, no further details are available on the part associated, as well as morphological and molecular confirmation of L. theobromae. This is the first report of the L. theobromae causing panicle blight disease of grapes in India. Further, understanding the host range for L. theobromae and its variation will help to draw suitable disease management strategies.

Lemko Carving Traditions as Part of Applied Art

The purpose of work is to identify the place of Lemko carving traditions in applied art, to describe peculiarities of Lemko carving traditions, and identify its leading artists. The research is related to the fact that Lemko national carpenters and carvers created real masterpieces, distinguished by uniqueness and originality of artistic forms. Methodology of research consists is applying comparative, historical, and logical methods. Scientific novelty of the work consists in the expansion of ideas about the place of Lemko carving traditions in applied art. The specialities of Lemko carving traditions have been shortly described, it has been resumed that Lemkos depicted floral ornaments on their products. It has been assumed that flat carving (plates, trays) probably arose, the products are combined from leaves of various trees, flowers, and bunches of grapes. Firstly, peasants decorated with floral ornament household items, for example, wooden boxes (chests), lizhnyks (woollen plaids), house tragars (decorative wooden beams). Soon, the masters transferred decorative elements to industrial souvenirs (plates, cassettes, and trays). It has been emphasised that one of the most outstanding masters of Rusyn wood carving in Lemko region is M. Orysyk (1885–1946) («Lemko-Rusyn» (1930s) is the most famous work) and V. Odrekhivskyi, the Honored Worker of Arts of Ukraine (1964), the member of the Union of Artists of Ukraine. Conclusions. Highly artistic Lemko carvings reflect the centuries-old traditions of carving, as well as the rich history of the formation and development of Lemko culture, which give us the opportunity to learn the true value of Lemkos. Woodcarving became a very popular type of artistic and decorative wood processing in Lemko region at the border of the 19th and 20th centuries and a significant part of applied art.
 Key words: Lemko carving traditions, carving, applied art, artists, products.

Diving in the big blue: Atypical Mott cells in rapidly fatal primary amyloidosis.

A 75-year-old man with no previous medical history presented at the emergency unit with asthenia, anorexia, weight loss, and progressively increasing dyspnea revealing acute heart failure (N-terminal pro b-type natriuretic peptide:16 667 ng/L; troponemia: 47 ng/L). Echocardiography showed a granular appearance of the myocardium and interventricular septal hypertrophy, suggestive of amyloidosis. Chemistry disclosed high levels of immunoglobulin kappa light chains at 1240 mg/L (lambda light chains 14 mg/L; κ/λ ratio 86) and a detectable but unquantifiable IgA κ peak. Myocardial biopsies pathology showed an endocardium thickened by fibrosis. Within the myocardium, the presence of interstitial fibrillary deposits stained by Congo red and birefringent in polarized light confirmed the diagnosis of cardiac amyloid light-chain (AL) amyloidosis. The search for other organ damage disclosed vegetative involvement with orthostatic hypotension, icteric cholestasis (bilirubin 51.8 mg/L), undocumented digestive involvement (diarrhea with evocative endoscopic lesions), and glomerulonephritis (urinary proteinuria 4 g/24 h with 3 g/24 h albumin and 1 g/24 h immunoglobulin light chains). A bone marrow aspirate displayed 17% of plasma cells. Interestingly, a particular pattern of huge atypical Mott cells was identified (Figure 1A,B). The latter had so many cytoplasmic inclusions that the cytoplasm, and the nucleus could not be discriminated. These atypical cells are however plasma cells with an endoplasmic reticulum loaded with immunoglobulins and should be integrated as such in cell counts. Classical plasma cells and typical Mott cells with a few Russel bodies were also present (Figure 1C) indicating a continuum in the shape of the diseased plasma cells. Treatment with cyclophosphamide–bortezomib–dexamethasone-daratumumab was initiated in emergency. Unfortunately, the patient died 3 days later, of multiple organ failure. No additional septic or toxic causes could be identified. Mott cells are activated plasma cells containing intracytoplasmic inclusions with a “bunch of grapes” appearance. These inclusions, called Russell bodies, are dilated cisternae of the endoplasmic reticulum containing condensed immunoglobulins. They were identified in 1901 by the surgeon F.W Mott in the brains of monkeys with trypanosomiasis. Mott cells have been described in various pathological conditions: reactive plasmacytosis, monoclonal gammopathies, high grade B-cell lymphomas, Wiskott-Aldrich syndrome, and von Recklinghausen neurofibromatosis. AL amyloidosis is caused by clonal expansion of plasma cells producing misfolded immunoglobulin light chains responsible for the deposition of insoluble fibrillar proteins in many organs. Those most frequently affected are the kidneys, heart, liver, and peripheral nervous system. AL amyloidosis is a rare disease, and its diagnosis is often delayed. Although associated with a poor prognosis, AL amyloidosis presents a heterogeneous clinical expression and evolution. To our knowledge, the presence of Mott cells has rarely been described in this pathology. KR, ME, and BLC wrote the manuscript. KR, ME, and BLC performed biological analyses. CS treated the patient. All authors critically reviewed the manuscript. The authors have no conflict of interest to disclose. The authors received no specific funding for this work. The research was conducted in accordance with the principles embodied in the Declaration of Helsinki and in accordance with local statutory requirements.