Bull Serum Albumin Research Articles

Copper phosphate-rotenone nanocomposites for tumor therapy through autophagy blockage-enhanced triphosadenine supply interruption and lipid peroxidation accumulation

Malignant tumor cells feature rapid metabolism and proliferation that need a large amount of energy to maintain these physiological activities. Blocking energy supply through mitochondria dysfunction is an alternative strategy but is hindered by the limited blockage efficacy and the repairation of cytoprotective autophagy. Herein, a novel kind of bull serum albumin (BSA)-coated rotenone (RO)-copper phosphate (CuPi) nanocompsites was prepared for tumor therapy through autophagy blockage-enhanced triphosadenine (ATP) supply interruption and lipid peroxidation (LPO) accumulation. When internalization by tumor cells, these formed BSA-RO-CuPi nanoparticles (NPs) degraded to release RO, Cu2+, and PO43−. RO acts on mitochondrial complex I and reduces the activity of Fe-S clusters, and Cu2+ is reduced into Cu+ by ferredoxin 1 (FDX1) to induce dihydrolipoamide S-acetyltransferase (DLAT) aggregation. Thus, mitochondria function is severely damaged to cut off ATP supply. Meanwhile, Cu2+ depletes glutathione (GSH) through a redox reaction and produces hydroxyl radicals through the Fenton-like reaction, inducing the generation of LPO. Importantly, PO43− weakens lysosomal acidity to disrupt the autophagy capacity of tumor cells, resulting in damage accumulation. In vivo data demonstrated that these developed BSA-RO-CuPi NPs show desirable antitumor capacity and emphasize the importance of mitochondria dysfunction in tumor treatment applications.

Highly ordered mesostructured flexible silica-based nanofiltration membrane with satisfactory acid, chlorine, and fouling resistances

In this report, a highly ordered mesostructured flexible silica-based nanofiltration membrane is fabricated using tetraethyl orthosilicate (TEOS) as a silica source and Pluronic F127 copolymer as a structure directing agent, and hydrolyzed polyacrylonitrile ultrafiltration membrane as a substrate by an evaporation induced self-assembly approach. The results show the fabricated silica/F127 (SF) composite membrane possesses ordered mesostructures with different space groups depending on the mass ratios of TEOS/F127. The membrane is negatively charged with an isoelectric point at pH 2.9, and it also has a very hydrophilic surface. The pure water permeability of the membrane is 32 Lm−2h−1bar−1. The membrane also has high rejections to methyl blue (100%) and multivalent salts (> 95%), and moderate rejection to divalent salt (78.5% for Na2SO4). The ordering of the mesostructure enhances the membrane compactness, favoring the improvement of the separation performance. Additionally, the membrane possesses satisfactory resistances to humic acid (HA) and bull serum albumin (BSA) fouling, and bacteria adhesion due to the smooth and hydrophilic surface. Additionally, the salt rejection is stable in 0.5 M HCl for 11 h and/or active chlorine solution (pH = 5) with 9000 ppm·h chlorination strength accompanied by an obvious increase in permeate flux. The membrane also exhibits satisfactory performance stability in a long-term (168 h) running.

Functionalized Graphitic Carbon Nitride Nanomaterials for Electrochemiluminescent Detection of Cancer Cells

Biosensors capable of detecting cancer cell quantity is significant for cancer pathological investigation and prognosis. Herein, a facile electrochemiluminescent (ECL) biosensor is developed for cancer cell detection by comprehensively utilizing the features of functionalized graphitic carbon nitride (g-CN) nanomaterials like well film-forming capability and functionalization level tunability. The solid-state ECL biosensor is fabricated via a simple dropping-drying method by subsequent deposition of g-CN nanosheets/graphene oxide nanocomposites (CNNS/GO) as ECL emitter, folate functionalized g-CN quantum dots (FA-CNQDs) as cancer cell capture agent, and bull serum albumin (BSA) as blocker to prevent nonspecific binding on glassy carbon electrodes (GCEs). The strong and stable ECL emission of CNNS/GO along with the tunability of FA content in FA-CNQDs endow the ECL biosensor with competitive sensitivity, which is able to detect folate receptor-positive cancer cells (HepG2) in the concentration range of 102 − 104 cells ml−1 under optimal condition. Additionally, the proposed ECL biosensor shows high specificity, reproducibility and long-term stability as well as good reliability towards serum sample detection.

A Membrane with Strong Resistance to Organic and Biological Fouling Using Graphene Oxide and D-Tyrosine as Modifiers.

Membrane fouling markedly influences the service life and performance of the membrane during the using process. Herein, hydrophilic polyvinylidene fluoride (PVDF) nanocomposite (P-GO-DAA) membranes with antifouling and anti-biofouling characteristics were fabricated by employing graphene oxide (GO) and different concentrations of D-Tyrosine. The structural properties of the prepared nanocomposite membranes as well as pure PVDF membranes were characterized using FTIR, XPS, SEM, AFM, and contact angle analysis. It was found that the introduction of GO fillers made an excellent antifouling performance compared to pure PVDF indicated by the pure water flux, flux recovery rate, and rejection rate during ultrafiltration experiments as a result of the formation of the hydrophilic and more porous membrane. In particular, the nanocomposite membranes showed an increased flux of 305.27 L/(m2·h) and the rejection of 93.40% for the mixed pollutants solution (including Bull Serum Albumin, Sodium Alginate, and Humic Acid). Besides, the outstanding anti-biofouling activity was shown by the P-GO-DAA membrane with the properties of D-Tyrosine for inhibiting biofilm formation during the bacterial adhesion experiments. Furthermore, the adhesion ratio of bacteria on the membrane was 26.64% of the P-GO-DAA membrane compared to 84.22% of pure PVDF. These results were confirmed by CLSM.

Enhanced Stability and Mechanical Properties of a Graphene–Protein Nanocomposite Film by a Facile Non-Covalent Self-Assembly Approach

Graphene-based nanocomposite films (NCFs) are in high demand due to their superior photoelectric and thermal properties, but their stability and mechanical properties form a bottleneck. Herein, a facile approach was used to prepare nacre-mimetic NCFs through the non-covalent self-assembly of graphene oxide (GO) and biocompatible proteins. Various characterization techniques were employed to characterize the as-prepared NCFs and to track the interactions between GO and proteins. The conformational changes of various proteins induced by GO determined the film-forming ability of NCFs, and the binding of bull serum albumin (BSA)/hemoglobin (HB) on GO’s surface was beneficial for improving the stability of as-prepared NCFs. Compared with the GO film without any additive, the indentation hardness and equivalent elastic modulus could be improved by 50.0% and 68.6% for GO–BSA NCF; and 100% and 87.5% for GO–HB NCF. Our strategy should be facile and effective for fabricating well-designed bio-nanocomposites for universal functional applications.

Fabrication of PDMS chips by laser engraving for protein enrichments

Abstract PDMS (Polydimethylsiloxane) chips are increasingly important for the application of fluorescence measurements due to their auto-fluorescence free, excellent transparency, and biocompatibility. However, the design of PDMS microfluidic chips requires to fabricate plenty of molds for structure optimization, resulting in high cost. In the present, PDMS chips with nafion membrane were fabricated by simple and low-cost method for bull serum albumin (BSA) enrichment. To optimize the laser cutting and bonding parameters, simulation models were established using Bilinear Kinematic and Mooney-Rivlin models, respectively. The influence of laser power and cutting speed on the width and depth of the micro-channels was investigated. And the effect of bonding pressure on the deformation of PDMS micro-channel and stress distribution near the micro-channels was also analyzed. The leakage test and BSA enrichment demonstrated the practicability and feasibility of the present fabrication method in this work.

A developed HPLC-MS/MS method to quantitate 5 steriod hormones in clinical human serum by using PBS as the surrogate matrix

Steroid hormones play an essential role in regulating physiological and reproductive development throughout the lifetime of an individual. One of the difficulties in determining endogenous substances is the lack of a blank matrix. Especially when the level of analytes is lower than the level in the so-called blank matrix. In the present study, an optimized HPLC-MS/MS method was developed and validated to quantify androstenedione (ASD), testosterone (Ts), dehydroepiandrosterone (DHEA), 5α-dihydrotestosterone (DHT), and progesterone (P) in serum samples from healthy people using PBS (pH = 7.4) as the blank surrogate matrix. Simultaneously, the method investigated the characteristics of NaCl, bull serum albumin, pure water as surrogate matrices for the analysis of steroid hormones. The data showed that the matrix effects of ASD, Ts, DHEA, DHT, and P in the same groups were not significantly different between PBS and twice charcoal-stripped serum (CS2S) as a blank surrogate matrix. Furthermore, the LLOQ using PBS as the blank matrix was up to 0.005 ng/mL for ASD, Ts, and P and 0.05 ng/mL for DHEA and DHT. The reference ranges of concentration (CPBS) of 5 steroid hormones were provided. Compared to the concentration with CS2S (CCSS) as the blank surrogate matrix, the relative biases (RBs) of Ts, DHT, P, and DHEA were finally stabilized at approximately −0.7%, −15%, −1.2%, and 9.2%, respectively. The results suggest that, in the cases of special required, the developed HPLC-MS/MS method can be used to determine the absolute concentration of 5 hormones in biological samples with PBS as the blank surrogate matrix.

Sustainable amorphous Fenton nanosystem for visualization-guided synergistic tumor elimination

Chemodynamic therapy (CDT) shows great potential in tumor treatment, in which tumor cells are killed by hydroxyl radicals (·OH) generated by Fenton-catalysis-mediated hydrogen peroxide (H2O2) disproportionation. Fabricating a high-performance tumor microenvironment (TME)-responsive Fenton nanocatalyst and realizing sustainable ·OH production are essential for effective tumor elimination but still challenging. Herein, we reported a sustainable amorphous Fenton nanosystem (SAFN) for visualization-guided synergistic tumor therapy, highlighting the superiority of cascade catalysis and the sustainability of the Fenton nanosystem. The SAFN was fabricated by rational integration of a polydopamine (PDA) core, an amorphous ferric phosphate (FePi) payload, glucose oxidase (GOx) decoration, and a bull serum albumin coating. In an acidic TME, Fe(III) ions were released on demand from FePi and reduced to high-activity Fe(II) by PDA. The reducing capacity of PDA was stronger under acidic conditions, due to C=O exposure. Fe(II) and H2O2 are sustainably supplied by the PDA-reducing and GOx catalytic chemistry, achieving long-lasting ·OH production for enhanced CDT (ECDT). Localized hyperthermia can be generated by PDA under near-infrared laser irradiation for low-temperature photothermal therapy (LT-PTT) in a GOx-induced energy-shortage TME. In addition, a remarkable antitumor effect is achieved by this sustainable nanosystem through the synergistic effect of the starvation/LT-PTT/ECDT strategy.

A comparison of effect mechanisms of chlorination and ozonation on the interfacial forces of protein at membrane surfaces and the implications for membrane fouling control

Pre-chlorination and pre-ozonation are the two most common pre-oxidation processes for fouling control of ultrafiltration membranes. However, few studies have explored and compared their effect mechanisms on membrane fouling behavior at the interfacial forces level. Accordingly, bull serum albumin (BSA) was used, and the changes in the interaction forces of BSA at the membrane surface after pre-chlorination and pre-ozonation were investigated. The results were combined with macro/micro analyses to unravel the effect mechanisms of chlorination and ozonation on membrane fouling behavior. Results demonstrated that the changes in the hydrophobic forces, hydrogen bonds and electrostatic repulsive forces between the membrane and BSA and between BSA molecules after pre-chlorination and pre-ozonation, were beneficial for membrane fouling control in theory, but not helpful. These changes in the non-covalent bonds appear to be completely masked by changes in disulfide bonds. The chlorination could break the disulfide bonds between BSA molecules, while ozonation could promote their formation. As a result, the deposition behavior of BSA onto the membrane surface and the structures of corresponding fouling layers after pre-chlorination were quite different from those after pre-ozonation. Finally, two different membrane fouling behaviors were observed that membrane fouling was definitively mitigated by pre-chlorination but enhanced by pre-ozonation.

Brush assisted layer-by-layer assembled lignin/polyelectrolyte membrane

A lignin/polyelectrolyte membrane was facilely fabricated by a novel brush assisted layer-by-layer (LBL) assembly approach using poly(allylamine hydrochloride) as a polycation, sodium lignin sulfonate (SLS) as a polyanion, and glutaraldehyde as a crosslinker. This approach favors the quick formation of an effective separation layer compared to the classical static LBL assembly. The obtained membrane exhibits high rejection to trivalent and tetravalent anion salts (>90%), along with high permeate flux (up to 66.3 L m−2h−1). The ultrathin separation layer (15 nm) and excellent hydrophilicity favor the enhancement of the permeate flux. The abundant hydrophilic groups of the outermost SLS layer and negatively charged surface endow the membrane with good fouling resistance to bull serum albumin. The membrane also shows good stability on the separation performance.

Impact of monovalent cations on the separation performance of graphene oxide membrane for different organic matters.

Graphene oxide (GO) membrane has become a promising membrane due to its advantages of high flux, good anti-pollution performance, and controllable cost. Since wastewater contains inorganic ions and organic substances, the effect of inorganic ions on the removal of organic matters has attracted attention. This study investigated the impact of monovalent cations (Na+ and K+) on the removal of different organic matters by GO membranes. The results showed that the removal by GO membranes of both macromolecular organic compounds (humic acid, sodium alginate, and bull serum albumin) and small molecular organic substances (new coccine, methyl orange, and methylene blue) were high (>75%). The coexisting Na+ caused the increase in flux and the decrease in removal of small molecular organic matters, while the impact on the removal of macromolecular organic matters was weak. The impact of the coexisting K+ was weaker than Na+. With the crosslinking of ethylenediamine, the structure of GO membrane was more stable. The impacts of Na+ on the flux and separation performance of crosslinked membranes were less than that of non-crosslinked membranes. This suggests that crosslinked GO membrane has more potential for practical wastewater treatment.

Preparation and antifouling performance of PVDF-DCOIT composite hollow fiber membranes

Membrane fouling is the main bottleneck that hinders the applications of membrane bioreactors (MBRs). 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT), as an environmentally-acceptable antifouling biocide, was mixed with Polyvinylidene fluoride (PVDF) to fabricate hollow fiber membrane via non-solvent induced phase separation (NIPS), which was able to effectively improve the antifouling performance of the membranes in this work. Overall research of the prepared membrane revealed that membranes with 3 wt% DCOIT exhibited the optimum antifouling performance. With the addition of DCOIT, hydrophilicity and pure water flux of 3 wt% DCOIT membranes maintained remarkable improvement by 22.9% and 64.6% than that of membranes without DCOIT. Meanwhile, the surface morphologies of 3 wt% DCOIT membranes were smoother than the control group in terms of SEM and AFM images, which was beneficial to alleviate membrane fouling. In antifouling experiments, the flux variation rate of membranes with 3wt% DCOIT filtrated in bull serum albumin, sodium alginate and humic acid solution were 81.42%, 54.25%, 50.5%, while membrane without DCOIT were 64.6%, 24.72% and 29%, respectively. Similar results were obtained by filtrating anaerobic sludge for 24 h. The flux variations of 3 wt% DCOIT membranes were 59.4%, 47.8% and 46.0%, respectively in three stages. However, the flux variations of membranes without DCOIT were 44.8%, 36.7% and 19.8%, respectively, which showed better antifouling ability and higher flux recovery efficiency The novel membranes would provide some theoretical basis and technical support for the rational combinations in elevating the overall antifouling properties of membranes.

Highly Efficient Dual-Polar Electrochemiluminescence from Au25 Nanoclusters: The Next Generation of Multibiomarker Detection in a Single Step

For the first time, simultaneous cathodic and anodic electrochemiluminescence (ECL) emissions of bull serum albumin stabilized Au25 nanoclusters (Au25 NCs) as highly efficient bipolar ECL probes were constructed for the single-step and synchronous determination of carcinoembryonic antigen (CEA) and mucin 1 (MUC1) based on different coreactant and coreaction accelerators. Under the potential cathodic scanning, TiO2 nanosheets (TiO2 NSs) as cathodic coreaction accelerators could catalyze the reduction of coreactant dissolved O2 for promoting cathodic ECL emission of Au25 NCs, while, under the potential anodic scanning, Cu2O@Cu nanoparticles (Cu2O@Cu NPs) as anodic coreaction accelerators could stimulate the oxidation of coreactant N,N-diethylethylenediamine (DEDA) for enhancing anodic ECL emission of Au25 NCs. Thus, the proposed strategy easily solved one main technical challenge of cross reactions of dual-luminophors for dual-biomarker detection, resulting in great sensitivity and accuracy toward the detection of CEA down to 0.43 pg/mL and MUC1 down to 5.8 fg/mL. As a proof of concept, this work realized single-step and simultaneous detection of dual biomarkers, which initiates a new thought in realizing a new generation of dual-biomarker ECL detection beyond the traditional ones in sensing analysis and diagnostic sensing.

Impacts of mono/divalent cations on the lamellar structure of cross-linked GO layers and membrane filtration performance for different DOM fractions

This study evaluated the effects of co-existing cations (Na+ or Ca2+) on the lamellar structure of cross-linked graphene oxide (GO) layers and GO modified membrane performance in terms of their fouling behaviours and retention for single-model organic matter, namely, bull serum albumin (BSA), sodium alginate (SA), humic acid (HA) and tannic acid (TA). In the absence of co-existing cations, the GO layers mitigated membrane fouling for large molecules (SA, BSA, and HA) but led to severer pore blocking for small molecules (TA) compared with pristine membrane. Na+ and Ca2+ altered the performance impacts of the GO modified membrane due to different interactions with the cross-linked GO layers. Low concentrations of Na+ (<0.4 mM) enlarged interlayer spacing of the GO layers and caused a decrease in flux after physical cleaning, but the GO layers maintained the uniform lamellar structure. High concentrations of Na+ (>0.4 mM) promoted the aggregation of cross-linked GO layers through charge shielding and reduced the uniformity of lamellar structure, which weakened the antifouling performance for large molecules and promoted the passage of small molecules through the membrane. However, Ca2+ complexed with GO sheets and reinforced the uniform lamellar structure of the GO layers, leading to a better antifouling performance for the filtration of large molecules than the pristine membrane but aggravated TA fouling.

Determination of freely dissolved polycyclic aromatic hydrocarbons in human serum using core-shell Fe3O4@polyacrylate magnetic microspheres by exclusive volume effect

Freely dissolved concentration is an important parameter for evaluating the bioavailability of compounds. Negligible-depletion solid-phase microextraction (nd-SPME) has been widely used for the determination of freely dissolved compounds but suffered from long equilibrium time. Multifunctional mesoporous composite microspheres have large specific surface area and therefore extraction equilibrium could be reached in a short time. In this study, a novel method was developed for quick determination of freely dissolved polycyclic aromatic hydrocarbons (PAHs) in human serum using core-shell polyacrylate-ferriferous oxide magnetic microspheres (Fe3O4@PA). Mass transfer of PAHs from sample solution to Fe3O4@PA was greatly increased owing to unique properties including large surface area (58.5 m2 g−1), high pore volume (0.10 cm3 g−1) and thin coating layer (50 nm). Freely dissolved PAHs can be selectively extracted because of the mesoporous structure of PA coating layer with uniform pore size of 7.08 nm. However, bound forms of PAHs would not be able to access into pore channels due to size exclusion. In comparison with long equilibration time (139 h) by nd-SPME, equilibrium can be reached within 29 min (t90%) using Fe3O4@PA as novel sorbents. The sorption coefficients (log KBSA) of PAHs to bull serum albumin (BSA) ranged from 3.36 to 4.87, which are consistent with the values measured by nd-SPME (log KBSA = 3.64–5.12). Finally, the freely dissolved PAHs (Cfree) measured by the proposed method (0.69–1.92 μg L−1) have a good agreement with that by nd-SPME (0.56–2.11 μg L−1), indicating that it is feasible for rapid determination of free forms of compounds in real samples by Fe3O4@PA.

Fluorescence enhancement of small squaraine dye and its two-photon excited fluorescence in long-term near-infrared I&II bioimaging.

Two-photon excited fluorescence (TPEF) plays an important role in bioimaging, the longer excitation wavelength improves its imaging depths, which gives us deeper biological information. Here, we reported the two-photon absorption of a small squaraine dye (SD), and we found that the TPEF of the small SD can be enhanced significantly using albumin, the TPEF of SD in water was enhanced 17.7 times by adding bull serum albumin (BSA) in the solution. Meanwhile, the cell imaging results indicated that the SD can enter cell effectively in less than 30 min and emit bright TPEF. Furthermore, the SD showed excellent stability against photobleaching in near-infrared II (1200 nm). The cytotoxicity experiment showed that the cytotoxicity of SD is relatively low. Our work demonstrates the excellent two-photon effect of SD in cells, potential application value of SD in two-photon bioimaging, protein detection and near infrared sensing.

Wear behavior of fluorinated diamond-like carbon (DLC) film in bull serum albumin (BSA) solutions

Fluorinated diamond-like carbon (DLC) films have good biocompatibility and bloodcompatibility. When they are used in cardiac valve prosthesis, the wear resistance has to be investigated in more detail. In this paper, the tribological behavior of fluorinated DLC films under different loads and sliding speeds in bull serum albumin (BSA) solutions were investigated by ball-on-disc friction tests. The results show that the proteins in BSA solutions promoted the DLC film adhesion failure because of the increased friction coefficients and accelerated corrosion rate. The fluorine doping can lower the corrosion rate of DLC film, thereby improving the adhesion stability and wear resistance of DLC in BSA solutions.

Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering

Hydrogels based scaffolds are very promising materials for a wide range of medical applications including tissue engineering and drug delivery. This study reports a covalently cross-linked composite hydrogel embedded with microspheres basing natural polysaccharides as a protein delivery system for soft tissue engineering. This biodegradable composite hydrogel derived from water-soluble chitosan and alginate derivatives upon mixing, without addition of chemical cross-linking agents. The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of N-succinyl chitosan (N-Chi) and oxidized alginate (OAlg), respectively. Meanwhile, gel-like microspheres were prepared with a diameter of 2–10 μm by conjugating sodium alginate with Ca2+ in an aqueous emulsion via the emulsion cross-linking technique. Bull Serum Albumin (BSA) was encapsulated into alginate gel microspheres and subsequently incorporated into OAlg/N-Chi hydrogels to produce a composite scaffold. In the current work, gelation rate, morphology, mechanical properties, swelling ratio, in vitro degradation and BSA release of the composite scaffolds were examined. The results show that mechanical and stable properties of gel scaffolds can be significantly improved by embedding alginate microspheres. The alginate microspheres can serve as a filler to toughen the soft OAlg/N-Chi hydrogels. Compressive modulus of composite gel scaffolds containing 0.5 mL volume of microspheres was 57.3 KPa, which was higher than the control hydrogel without microspheres. Moreover, the controlled release of BSA encapsulated within this composite hydrogels showed significantly lower rate when compared with control hydrogel or microspheres alone. These characteristics provide a potential opportunity to use this injectable composite gel scaffold in protein delivery and soft tissue engineering applications.

Transport of biochar colloids in saturated porous media in the presence of humic substances or proteins

Application of biochar in the field has received considerable attention in recent years, but there is still little known about the fate and transport of biochar colloids (BCs) in the subsurface. Natural organic matter (NOM), which mainly consists of humic substance (HS) and proteins, is ubiquitous in the natural environment and its dissolved fraction is active and mobile. In this study, the transport of BCs in saturated porous media has been examined in the presence of two HS (humic and fulvic acids) and two proteins. Bull serum albumin (BSA) and Cytochrome c (Cyt) were selected to present the negatively and positively charged protein, respectively. At low and high salt concentration and different pH conditions, the transport of BCs was strongly promoted by HS. HS significantly increased the mobility of BCs in porous media under both low and high salt conditions due to the enhanced electrostatic repulsion and modification of surface roughness and charge heterogeneity. While BC mobility in porous media was suppressed by both BSA and Cyt in the low salt solution, the presence of BSA largely promoted and Cyt slightly enhanced the transport of BCs in high salt solutions. BSA and Cyt adsorption onto BC surface decreased the negative charge of BC and resulted in a less repulsive interaction in low salt solutions. In high salt solutions, the adsorbed BSA layers disaggregated BCs and reduced the strength of the interaction between BC and the sand. Adsorbed Cyt on BCs caused more attractive patches between BC and sand surface, and greater retention than BSA.

BSA modified, disulfide-bridged mesoporous silica with low biotoxicity for dual-responsive drug delivery

Abstract Siliceous materials based intelligent drug delivery systems (DDS) have attracted numerous attentions. Unfortunately, their intrinsic biologic inertia and non-degradability are critical issues need to be addressed because it hampers further clinical translation application. Herein, disulfide-bridged mesoporous silica nanoparticles (designed as mSiO2(s-s)) have been synthesized through nucleophilic substitution reaction, which have biodegradability in simulative tumor reducing conditions. Furthermore, folic acid (FA) decorated bull serum albumin (BSA) has been modified onto the surface of mSiO2(s-s) to improve tissue biocompatibility, prevent anticancer drugs leakage and endow effective targeting capacity. Therefore, the nanoparticles present good biodegradability, tissue biocompatibility, tumor cell targeting capacity and pH/glutathione (GSH) dual-responsive drug release capacity. This novel drug nanocarrier possesses biosafety and effective anti-cancer strategy, provides significant promising in future biomedical application.