Abstract Previous theoretical and simulation results indicate that anisotropic porous materials have the potential to reduce turbulent skin friction in wall-bounded flows. This study experimentally investigates the influence of anisotropy on the drag response of porous substrates. A family of anisotropic periodic lattices was manufactured using 3D printing. Rod spacing in different directions was varied systematically to achieve different ratios of streamwise, wall-normal, and spanwise bulk permeabilities ($$\kappa _{xx}$$ κ xx , $$\kappa _{yy}$$ κ yy , and $$\kappa _{zz}$$ κ zz ). The 3D printed materials were flush-mounted in a benchtop water channel. Pressure drop measurements were taken in the fully developed region of the flow to systematically characterize drag for materials with anisotropy ratios $$\frac{\kappa _{xx}}{\kappa _{yy}} \in [0.035,28.6]$$ κ xx κ yy ∈ [ 0.035 , 28.6 ] . Results show that all materials lead to an increase in drag compared to the reference smooth wall case over the range of bulk Reynolds numbers tested ($$\hbox {Re}_b \in [500,4000]$$ Re b ∈ [ 500 , 4000 ] ). However, the relative increase in drag is lower for streamwise-preferential materials. We estimate that the wall-normal permeability for all tested cases exceeded the threshold identified in previous literature ($$\sqrt{\kappa _{yy}}^+> 0.4$$ κ yy + > 0.4 ) for the emergence of energetic spanwise rollers similar to Kelvin–Helmholtz vortices, which can increase drag. The results also indicate that porous walls exhibit a departure from laminar behavior at different values for bulk Reynolds numbers depending on the geometry. Graphical abstract