zF test showed that there was a significant difference between treatment plots, and the least significant difference (LsD) was calculated. Lilium japonicum Thunb., pink lily or lovely lily, is mainly grown in central Japan for use in religious ceremonies and as an ornamental. The wild population of L. japonicum is decreasing, and we are trying to revive its. cultivation using plant tissue culture techniques. Only a report by Shoyama et al. (1988) details work on L. japonicum. Although lilies usually are propagated by scaling, a technique that produces bulblets from each bulb scale, depending on the cultivar, other organs of lily plants have regenerative capacity, e.g., leaves (Niimi and Onazawa, 1979), peduncles and petals (Takayama and Misawa, 1979); and bulblets (Shoyama et al., 1988). Shoot apices have been used as explants for bulblet regeneration (Sheridan, 1968); however, the bulblets regenerated from callus may have undergone