The research was carried out at the research station of the College of Agriculture - University of Kufa during the growing season of 2022-2023 to demonstrate the effect of biofertilizers and phosphate fertilization on the growth of Iris flowers. Bacillus subtilis, Pseudomonas fluorescens, Azotobacter chroococcum by injecting the bacteria into the culture medium before planting the bulbs, and the second was foliar spray using superphosphate Nano-fertilizer at levels 0, 1, or 2 mg/L. The bulbs were cultivated on 10/24/2022 in a factorial experiment with three replications as Randomized Complete Block Design (R.C.B.D). Results showed that when phosphate Nano-fertilizer concentrations rose, so did indicator levels. It was also discovered that regardless of the type of bacteria used for fertilization, the results were the same: an increase in the growth markers of interest. In contrast to most Single and nested transactions, the results showed that the bacteria A. chroococcum at the treatment of P2 fertilization (2 mg/L) recorded the highest values for plant height, number of leaves, vegetative dry weight, number of bulbs, bulb diameter, and root dry weight. In comparison to other varieties of bacteria at the same stage of fertilization, however, it showed no significant differences.