Buckwheat Research Articles

Impact of direct and indirect ingestion of six systemic pesticides on the parasitoid Anagyrus vladimiri

Anagyrus vladimiri (Hymenoptera: Encyrtidae) is the main parasitoid of Planococcus citri (Hemiptera: Pseudococcidae), which is one of the most important pests of citrus crops in the Mediterranean Basin. Pesticide residue on trees and in the nectar of wildflowers growing in citrus orchards might cause side effects in beneficial insects. In the present study, we assessed the effects of six systemic pesticides (acetamiprid, dimethoate, cyantraniliprole, flupyradifurone, spirotetramat and sulfoxaflor) on the survival and progeny of A. vladimiri. Anagyrus vladimiri fed on the pesticide solutions and on the nectar of Fagopyrum esculentum Moench plants that had been watered with a solution containing these pesticides. Acetamiprid, dimethoate, flupyradifurone and sulfoxaflor increased female mortality more than the control (water) after 6h; after A. vladimiri consumed these pesticides, female mortality ranged from 62% to 73%. Cyantraniliprole had no immediate toxic effect on parasitoid survival but caused mortality after 15 days. All tested pesticides except flupyradifurone and spirotetramat reduced parasitoid progeny. After six days of feeding on F. esculentum nectar of plants watered with a pesticide solution, significantly increased female mortality was observed with dimethoate (96%), spirotetramat (80%), acetamiprid (62%), cyantraniliprole (62%) and sulfoxaflor (50%). Progeny was reduced in females that fed on plants treated with spirotetramat and dimethoate. These results demonstrate that some of these insecticides can have lethal or sublethal effects on A. vladimiri by both routes of exposure, via direct or indirect pesticide consumption through the nectar of treated plants.

Appropriate Concentration of Rice Straw Decomposition Liquid Prompted Germination and Seedling Growth of Common Buckwheat

Appropriate Concentration of Rice Straw Decomposition Liquid Prompted Germination and Seedling Growth of Common Buckwheat

Morphological and molecular characterization of Heterodera ripae, a new record cyst nematode in the rhizosphere soil of Fagopyrum esculentum

Numerous plant parasitic nematodes (PPNs) have the potential to inflict considerable damage on agricultural crops. Through a comprehensive survey aimed at identifying PPNs affecting crops, cyst nematodes were isolated from the rhizosphere soil of buckwheat (Fagopyrum esculentum). Employing both molecular and morphological techniques, this cyst nematode was conclusively identified as Heterodera ripae. Notably, this represents the first documented occurrence of this particular cyst nematode species within the rhizosphere soil of F. esculentum.

Temporal and fertilizer-dependent dynamics of soil bacterial communities in buckwheat fields under long-term management

This study integrated bacterial community and soil chemicals to characterize the soil ecosystem in an open upland field managed by six controlled fertilizer programs using the minimum amount of pesticides. Amplicon sequencing the 16S rRNA gene revealed that inorganic nitrogen fertilizer and compost altered the diversity and structure of the soil bacterial community throughout buckwheat (Fagopyrum esculentum Moench ‘Hitachiakisoba’) cultivation. The bacterial community comprised three clusters that contained bacteria that are prevalent in soils fertilized with nitrogen (cluster 1, 340 taxa), without nitrogen and compost (cluster 2, 234 taxa), and with compost-fertilized (cluster 3, 296 taxa). Cluster 2 contained more taxa in Actinobacteriota and less in Acidobacteriota, and cluster 3 contained more taxa in Gemmatimonadota compared with the other clusters. The most frequent taxa in cluster 1 were within the Chloroflexi phylum. The bacterial community structure correlated with soil chemical properties including pH, total organic carbon, SO42−, soluble Ca2+. A co-occurrence network of bacterial taxa and chemicals identified key bacterial groups comprising the center of a community network that determined topology and dynamics of the network. Temporal dynamics of the bacterial community structure indicated that Burkholderiales were associated with buckwheat ripening, indicating plant-bacteria interaction in the ecosystem.

Summer cover crop and temporary legume-cereal intercrop effects on soil microbial indicators, soil water and cash crop yields in a semi-arid environment

ContextFew studies have investigated summer cover crops as a replacement for bare fallow in semi-arid temperate regions, and summer cover crop impacts on soil biological function, water balances and subsequent winter crop yields in these regions remain largely unknown. ObjectiveThe 4-year field study aimed to determine the consequences of replacing a summer fallow with a summer growing cover crop on soil function, water balances and subsequent winter cash crop yields in a semi-arid region (486 mm mean annual rainfall) of the Australian southern cropping zone. MethodsA canola (Brassica napus)-wheat (Triticum aestivum L.) rotation with summer fallow was compared to the same rotation with either i) a single species (buckwheat, Fagopyrum esculentum L.) summer cover crop; ii) a four-species mixed brassica + cereal summer cover crop or iii) a cropping sequence with a temporary legume intercrop in the wheat phase of the rotation. Cover crop biomass production, soil water to a depth of 90 cm at cash crop sowing, crop yields and soil functional indices were quantified. ResultsCover crops produced 500–700 kg biomass ha−1 in the 2020 and 2021 seasons and 1000–2400 kg biomass ha−1 in 2022. The four-species mixed cover crop significantly lowered soil water (around 30 mm; P < 0.05) at the time of sowing the cash crop compared to the control and resulted in 15% reduction (P = 0.12) in canola seed yield in 2020, but no significant effect of cover crops of soil water and mineral N at sowing, or cash crop yields, were observed in subsequent seasons. In 2021 the temporary vetch intercrop produced > 1000 kg ha−1 biomass over 9 weeks, and led to a (non-significant) 10% wheat yield reduction. There was no significant effect of cover crop or temporary intercrop treatments on soil functions in the 2020 or 2021 seasons, but activities of several soil enzymes (leucine aminopeptidase, arylsulfatase and B-glucosidase; P < 0.05) and water-soluble carbon (P = 0.059) were significantly higher in the summer cover crop plots compared to control and intercrop plots in March 2022 following termination of the cover crops. These changes coincided with a significant increase in saturated hydraulic conductivity in the four-species cover crop plots in March 2022 compared to control plots. ImplicationsThe study did not provide any compelling evidence for benefits of summer cover crops over fallow in the short term (3–4 years). The relatively low cover crop biomass production over the 4-year study suggests summer cover crops are unlikely to substantially improve soil carbon levels in temperate, semi-arid cropping systems.

Evolutionary analysis of MADS-box genes in buckwheat species and functional study of FdMADS28 in flavonoid metabolism

The MADS-box gene family is a transcription factor family that is widely expressed in plants. It controls secondary metabolic processes in plants and encourages the development of tissues like roots and flowers. However, the phylogenetic analysis and evolutionary model of MADS-box genes in Fagopyrum species has not been reported yet. This study identified the MADS-box genes of three buckwheat species at the whole genome level, and conducted systematic evolution and physicochemical analysis. The results showed that these genes can be divided into four subfamilies, with fragment duplication being the main way for the gene family expansion. During the domestication process from golden buckwheat to tartary buckwheat and the common buckwheat, the Ka/Ks ratio indicated that most members of the family experienced strong purification selection pressure, and with individual gene pairs experiencing positive selection. In addition, we combined the expression profile data of the MADS genes, mGWAS data, and WGCNA data to mine genes FdMADS28/48/50 that may be related to flavonoid metabolism. The results also showed that overexpression of FdMADS28 could increase rutin content by decreasing Kaempferol pathway content in hairy roots, and increase the resistance and growth of hairy roots to PEG and NaCl. This study systematically analyzed the evolutionary relationship of MADS-box genes in the buckwheat species, and elaborated on the expression patterns of MADS genes in different tissues under biotic and abiotic stresses, laying an important theoretical foundation for further elucidating their role in flavonoid metabolism.

Elimination of zero-repeat subunit in allergenic seed protein 13S globulin using the novel allele GlbNB2 in common buckwheat (Fagopyrum esculentum Moench)

Common buckwheat (Fagopyrum esculentum Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The GlbNC allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in GlbNC homozygotes. Meanwhile, the GlbNB2 has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in GlbNB2 homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of GlbNB2 was more rigid than that of GlbNC. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development.

Development and validation of an analytical method based on QuEChERS followed by UHPLC–ToF-MS for the determination of tropane alkaloids in buckwheat (Fagopyrum esculentum L.) and buckwheat products

A method was developed for the determination of tropane alkaloids (TAs), including atropine, scopolamine, anisodamine and homatropine in buckwheat and related products. This work presents an optimised methodology based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction procedure followed by ultra-high performance liquid chromatography combined with time-of-flight mass spectrometry for the determination of TAs (atropine, scopolamine, anisodamine and homatropine) in buckwheat samples. The analytical methodology was successfully validated, demonstrating good linearity, low limit of quantification, repeatability (RSDr < 15%), inter-day precision (RSDR < 19%) and recovery (74–113%). Finally, 13 commercial samples of buckwheat were analysed and the results demonstrated that they were in compliance with the current European regulations regarding TAs.

Neutraceutical and Pharmaceutical Potential of Fagopyrum esculentum (Linn.) Moench.

Buckwheat is a gluten-free pseudocereal grown annually in hilly regions of India. It belongs to family Polygonaceae and genus Fagopyrum. Buckwheat is adaptable to extreme cold temperature, stress conditions of water making it a sustainable crop. In India, twenty species of buckwheat are cultivated across various hilly regions. Out of these only nine species have desirable nutritional value and two are commonly grown. They are Fagopyrum esculentum (Common buckwheat) and Fagopyrum tartaricum (Tartary buckwheat). Buckwheat has good amount of nutrients and many health benefits. Buckwheat has been used in the gluten free diet for those suffering from celiac disease. It can be used for the prevention of obesity and diabetes as they have high total dietary fibre and soluble dietary fibre. It is commonly used grain in the name of Kuttu in Indian society during fasting. The present study aims at establishing the Neutraceutical and Pharmaceutical utility of potential alternative to conventional food grains as mentioned in the contemporary texts.

Variation of glyphosate spray volume in the drying of ground cover plants with an unmanned aerial vehicle

The presence of straw on the soil is crucial for enhancing its physical, chemical, and biological conditions in no-tillage cropping systems, while contributing to weed control. This study aimed to determine the optimal spray volume for desiccating ground cover plants using glyphosate applied by an unmanned aerial vehicle (UAV). Treatments included an untreated control and glyphosate spraying at 5, 10, 15, 20, and 25 L ha−1 spray solution. The study was conducted in three fields in the State of Paraná, Brazil. Spraying variables were assessed using water-sensitive paper and the e-Sprinkle software, and included, covered area (%), recovered spray volume (L ha−1), droplet impact density, coefficient of variation for droplet diameter (CV%), and relative amplitude (SPAN). Herbicidal efficacy and dry biomass of cover plants were also evaluated. Increasing spray volume improved covered area, recovered volume, droplet impact density, and droplet diameter-spectrum variation but had no effect on droplet spectrum homogeneity, control of black oat (Avena strigosa), turnip (Raphanus sativus), or buckwheat (Fagopyrum esculentum). Spray volumes ranging from 5.0 to 25 L ha−1, applied with a UAV, provided satisfactory desiccation percentages of ground cover plants irrespective of species or location. Thus, for desiccating ground cover plants with glyphosate applied by UAV, a volume as low as 5.0 L ha−1 is effective.

Exogenous melatonin improves germination rate in buckwheat under high temperature stress by regulating seed physiological and biochemical characteristics.

The germinations of three common buckwheat (Fagopyrum esculentum) varieties and two Tartary buckwheat (Fagopyrum tataricum) varieties seeds are known to be affected by high temperature. However, little is known about the physiological mechanism affecting germination and the effect of melatonin (MT) on buckwheat seed germination under high temperature. This work studied the effects of exogenous MT on buckwheat seed germination under high temperature. MT was sprayed. The parameters, including growth, and physiological factors, were examined. The results showed that exogenous MT significantly increased the germination rate (GR), germination potential (GP), radicle length (RL), and fresh weight (FW) of these buckwheat seeds under high-temperature stress and enhanced the content of osmotic adjustment substances and enzyme activity. Comprehensive analysis revealed that under high-temperature stress during germination, antioxidant enzymes play a predominant role, while osmotic adjustment substances work synergistically to reduce the extent of damage to the membrane structure, serving as the primary key indicators for studying high-temperature resistance. Consequently, our results showed that MT had a positive protective effect on buckwheat seeds exposed to high temperature stress, providing a theoretical basis for improving the ability to adapt to high temperature environments.

Effects of Different Combinations of Red and Blue Light on the Edible Organ Morphology and Quality of Buckwheat (Fagopyrum esculentum Moench) Microgreens

Buckwheat microgreens are rich in nutrients and have a unique flavor that is favored by consumers. The light environment is closely related to the growth and development of the plant. In order to study the effects of treatments with different combinations of red and blue light on the edible organ morphology and nutritional quality of buckwheat microgreens, five experimental treatments were designed, with energy ratios of red light to blue light of 5:1 (R5B1), 3:1 (R3B1), 1:1 (R1B1), 1:3 (R1B3) and 1:5 (R1B5), respectively, and a white light treatment used as the control (CK). The results showed that different combination treatments of red and blue light had obvious effects on the growth of buckwheat microgreens. The hypocotyl length and main root length of buckwheat microgreens treated with a high proportion of red light (R5B1) were obviously higher than those of other treatment designs. However, contents of soluble protein, chlorophyll, rutin and total flavonoids in buckwheat microgreens showed an increasing trend with an increase in the proportion of blue light. Considering the fresh weight, dry weight and quality indexes of the edible organ, the combination of red light and blue light with a ratio of 1:1 was most suitable for buckwheat microgreen production. The results could provide a reference for the production of buckwheat microgreens.

Enhanced photosynthesis, transpiration regulation, water use-efficiency and growth in buckwheat outperforms wheat response to high [CO2], high temperature and drought

The rising atmospheric [CO2] levels will increase global temperature and drought events, threatening wheat (Triticum aestivum) production. In recent years, buckwheat (Fagopyrum esculentum) has emerged as an alternative crop to wheat under extreme drought. However, it is essential to understand the ability of these species to withstand future climatic conditions where drought and high temperature will occur simultaneously in a high [CO2] environment. Since the mitigating effect of high [CO2] is strongly influenced by the severity of the stress, we compared the response of wheat and buckwheat, differently sensitive to drought, to future climatic scenarios. In wheat, high temperature and high [CO2] passively decreased water potential, as shown by the higher dehydration. Likewise, future drought extremely increased water requirements, causing extreme reductions in the photosynthetic rate and in the quantum yield of PSII, as well as changes in the antioxidant metabolism. Conversely, buckwheat maintained optimal hydration levels, promoted higher photosynthetic rates and increased water-use efficiency under the combination of high [CO2] and high temperature, with and without drought. The improved response of buckwheat was attributed to an enhanced stomatal regulation and water-use efficiency, and resulted in an outperforming growth under future climatic conditions compared to the growth in wheat. Our research highlights the promising potential of buckwheat as an alternative crop to wheat under future climatic scenarios.

The inhibitory properties of the fagopyrum esculentum groats boiling extracts on corrosion of the mild steel in acidic environments

The inhibitory properties of the fagopyrum esculentum groats boiling extracts on corrosion of the mild steel in acidic environments

A new approach for modelling weed suppression of cover crops

AbstractCover cropping plays an important role in Integrated Weed Management (IWM). Weed suppression of cover crops occurs by competition, physical barrier on the soil and allelopathy. Under favourable growing conditions, cover crops can provide similar weed suppression in the autumn‐to‐spring period as repeated stubble tillage and non‐selective herbicides. The objective of this study was to test if cover crop species with higher allelopathic potential can suppress weeds in the field with less shoot biomass than cover crop species with lower allelopathic effects. Data of cover crop shoot biomass, percentage of weed suppression in the field and reduction of weed seed germination in petri‐dish bioassays treated with cover crop extracts were analysed from 11 experiments. Weed suppression in relation to cover crop biomass was better predicted by a Weibul three‐parameter model than with the Cousens model. Raphanus sativus var. oleiferus, Sinapis alba, Fagopyrum esculentum and Phacelia tanacetifolia needed at least three‐fold more biomass than Avena strigosa for 80% weed suppression. Vicia sativa had the lowest weed reduction of only 62%. Aqueous extracts of A. strigosa had the highest inhibiting effect on weed seed germination followed by R. sativus. The lowest effect was observed for P. tanacetifolia. It is assumed that for A. strigosa allelopathy strongly contributes to weed suppression whereas for P. tanacetifolia and other cover crops, competition plays the major role for weed suppression. The model can help growers and consultants to mix cover crop species for the autumn‐to‐spring period. Under dry conditions, species such as A. strigosa can provide high weed suppression with low biomass. Under moist conditions, rapidly growing cover crops such as S. alba suppress most weeds and deliver high biomass.

Flavor Chemical Research on Different Bee Pollen Varieties Using Fast E-Nose and E-Tongue Technology.

Bee pollen, derived from various plant sources, is renowned for its nutritional and bioactive properties, aroma, and taste. This study examined the bee pollen with the highest yield in China obtained from four plant species, namely Brassica campestris (Bc), Nelumbo nucifera (Nn), Camellia japonica (Cj), and Fagopyrum esculentum (Fe), using fast e-nose and e-tongue technology to analyze their flavor chemistry. Results showed substantial differences in scent profiles among the varieties, with distinct odor compounds identified for each, including n-butanol, decanal, and ethanol, in Bc, Nn, and Cj, respectively. The primary odorants in Fe consist of E-2-hexen-1-ol and (Z)-3-hexen-1-ol. Additionally, e-tongue analysis revealed seven distinct tastes in bee pollen samples: AHS, PKS, CTS, NMS, CPS, ANS, and SCS, with variations in intensity across each taste. The study also found correlations between taste components and specific odor compounds, providing insights for enhancing product quality control in bee pollen processing.

Effect of pericarp removal on buckwheat seed respiration and germination under submerged conditions

ABSTRACT Excessive soil moisture (ESM) is a critical factor limiting the productivity of buckwheat (Fagopyrum esculentum Moench) because ESM inhibits its germination. This study investigated the effects of pericarp and seed respiration on reduced buckwheat germination caused by submergence. Seeds were submerged prior to germination testing under aerobic conditions. The effects of days of submergence treatment and pericarp removal on germination percentage were significant. The germination percentage in the 3-day submergence treatment was low in seeds with pericarp (P+) but was significantly improved in seeds without pericarp (P−). Pericarp removal also improved the germination percentage of non-submerged seeds. Pericarp thickness and pericarp/seed dry weight ratio (PWR) were not significantly correlated with the germination percentage in 3-day submerged P+. However, both were significantly negatively correlated with the germination percentage in 1-day submerged P+. Seed respiration rate in water, measured on seeds not submerged before measurement, was significantly higher in P− than in P+; that of P− increased with time, while that of P+ showed a decreasing trend with time beyond the first 24 h. Seed respiration rate during the first 24 h was significantly negatively correlated with germination percentage in 3-day submerged P+. A negative influence of pericarp thickness and PWR on the seed respiration rate of P+ during the first 24 h was suggested. Suppressed seed respiration by the pericarp is probably a cause of inhibited buckwheat germination under ESM conditions.

Effect of microwave treatment on phytoconstituents of buckwheat (Fagopyrum esculentum) grains with special emphasis on allergenic proteins

Effect of microwave treatment on phytoconstituents of buckwheat (Fagopyrum esculentum) grains with special emphasis on allergenic proteins

Potential relation between starch granule-associated proteins and retrogradation properties of buckwheat starch

To elucidate the effect of starch granule-associated proteins (SGAPs) on retrogradation properties of buckwheat starch, the retrogradation properties of Tartary buckwheat starch (TBS) and common buckwheat starch (CBS) before and after removal of SGAPs were systematically investigated, with wheat starch (WS) as reference. A significant decrease in gel strength of starches and density of starch aggregates were observed after removing SGAPs. The results were in line with the changes in retrogradation enthalpy of starches and short-range ordered structure of starch aggregates. After removing SGAPs, the retrogradation enthalpy of TBS decreased from 4.16 J/g to 3.74 J/g, CBS decreased from 4.05 J/g to 3.35 J/g and WS decreased from 3.27 J/g to 2.81 J/g, respectively. Taken together the results of LF-NMR, FTIR and rheological analysis, it can be concluded that SGAPs could promote the hydrogen bond interactions between starch molecules by competitively binding with water molecules, enhancing the rearrangement of starch molecules and forming a more ordered structure. Overall, the study suggested that the presence of SGAPs could enhanced the interaction between starch molecules chains, thus accelerated the retrogradation process. The research results provide more information about SGAPs in buckwheat starch and support further study for manipulation of starch properties.

Odranci and buckwheat groats

The village of Odranci (Prekmurje, Slovenia) is still remembered for its production of buckwheat groats. A special feature were the groats sellers, mainly women, who carried buckwheat groats (kasha or kaša) on foot or by bicycle from house to house or to fairs in various villages in the surrounding area, and further afield.