BSF Larvae Research Articles

Potential the Black Soldier Fly (Hermetia illucens) in Feed Formulation for Growth of Common Carp (Cyprinus carpio L.)

The utilization of maggot as larvae from black soldier fly is an alternative substitution of fish meal as the primary protein source in fish feed. Maggot is cultivated in 3 different cultivation, the medium used is organic waste, tofu waste, and wheat pollard. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 replication. This study aims to determine the effect of various types of maggot growth media, 1) with maggot biomass parameters, containers, and growing media conditions maggot, 2) then, exact proximate composition, total amino acids content of the prepupae samples were determined. Based on the results of the analysis, each dose of treatment (media) has an influence on the different maggot biomass. The best media treatment results are pollard flour. The proximate analysis showed that BSF larvae had a protein content of 50,88% and also fat content of 15%.

Optimisation and characterisation studies of biodiesel production from black soldier fly larvae fed by soya residue

In this study, a two-step transesterification process was applied to convert crude BSF larval oil into biodiesel. The effects of methanol:oil (molar ratio), catalyst amount (weight %), reaction time (minutes) and temperature (°C) on biodiesel production were investigated. Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimise experimental data obtained from these four variables. The prediction of biodiesel yield was made by employing a quadratic model, whereby the R2 values were greater than 0.99. Based on optimisation studies, a combination of an application of petroleum ether and reaction time of 6 hours was the best to extract crude larval oil with 47% of lipid yield. Meanwhile, methanol:oil molar ratio of 12:1, catalyst (NaOH) concentration of 1.0 weight %, a reaction time of 32 minutes and temperature of 60°C were required to obtain biodiesel with 96% of production yield from BSF crude larval oil. Both BSF crude larval oil and biodiesel were characterised using several analytical instruments such as Fourier Transform Infrared (FTIR) spectrometer and Gas Chromatography – Flame Ionisation Detector (GC-FID). Overall, findings from this study highlight the potential to produce eco-friendly biodiesel from BSF larvae reared on soya residue.

Rearing substrate impacts growth and macronutrient composition of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae produced at an industrial scale

Organic waste is a rapidly increasing problem due to the growth of the agricultural production needed to meet global food demands. Development of sustainable waste management solutions is essential. Black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) (BSF), larvae are voracious consumers of a wide range of organic materials ranging from fruits and vegetables to animal remains, and manure. Thanks to this ability and considering the larval high protein and lipid content, BSF larvae are a useful additive in animal feeds and biodiesel production. Unfortunately, the feasibility of using the black soldier fly as a tool for waste valorization and feed production has primarily been investigated at the benchtop scale. Thus, mobilization of current practices to an industrial scale is challenging because scaling up from small laboratory studies to large industrial studies is not necessarily linear. The goal of this study was to demonstrate the ability of the BSF to recycle organic waste at an industrial scale. To accomplish this goal, three organic waste streams were used (e.g., apples, bananas, and spent grain from a brewery) to test six diet treatments (1) apple, (2) banana, (3) spent grain, (4) apple and banana, (5) apple and spent grain, and (6) banana and spent grain. Working at scale of 10,000 BSF larvae life history traits, waste valorization, protein and lipid profiles were measured for each diet treatment. Differences were recorded across all variables, except substrate conversion, for larvae fed on fruit and spent grain (alone or with fruit). Growth rate significantly differed across treatments; larvae reared on spent grain grew twice as fast as those fed apples alone, but those reared on the apple and spent grain mixture produced twice as much insect biomass. However, it should be noted that larvae resulting from the apple diet contained 50% more fat than larvae fed the fruit and spent grain mixtures. Commonly-available organic wastes were successfully used at an industrial scale to produce BSF larvae that have the potential to substitute other sources of protein and lipids in different industrial applications. Industrialization efforts are encouraged to assess these impacts when integrating diverse ingredients into larval diets as a means to more precisely predict output, such as larval development time and final larval biomass.

Chemical food safety of using former foodstuffs for rearing black soldier fly larvae (Hermetia illucens) for feed and food use

Black soldier fly (BSF, Hermetia illucens) larvae is considered one of the insect species with great potential for large-scale production as feed and food. For this to become economically feasible and to contribute to a circular economy, BSF larvae should be reared on substrates with little or no alternative use for feed and food production. One such class of alternative substrate sources consists of former food products. However, BSF larvae may accumulate chemical contaminants from the substrate, which may originate from the foodstuff and/or the packaging materials. This study aimed to investigate the possible presence of chemical contaminants in BSF larvae being reared on former foodstuff substrates at both laboratory and industrial scale. Four experimental treatments were set up: with meat or vegetarian, and containing between 3-6% of either plastic or paperboard carton packaging material. Four-day old BSF larvae were reared for seven days on these substrates. Concentrations of heavy metals, mineral oil hydrocarbons, dioxins and PCBs, and polycyclic aromatic hydrocarbons were determined in the substrate, residual material, and the larvae. Results suggest that BSF larvae can be reared on former food products containing traces of packaging materials, without negative effects on their growth or survival. Bio-accumulation was observed for most of the tested contaminants, in particular for mineral oils and cadmium, which had a bio-accumulation rate of, respectively, about five and 20. However, none of the concentrations of the analysed contaminants in the substrate and the larvae exceeded the respective legal limits in the EU. Results of this pilot study were promising. As a next step, more different former food products should be investigated in future research.

BIOCONVERSION OF MUNICIPAL ORGANIC WASTE USING BLACK SOLDIER FLY LARVAE INTO COMPOST AND LIQUID ORGANIC FERTILIZER

Bioconversion of municipal organic waste using Black Soldier Fly larvae provides potential benefits. Apart from reducing municipal solid waste, BSF larvae (maggot) offer valuable added value as animal feed. The purpose of this study was to analyze the potential of BSF larvae in the bioconversion process of municipal organic waste, to analyze the quality of the compost produced and to analyze the nutrition content of BSF larvae (maggot). BSF eggs in hatching are covered with gauze which are given organic waste feed, then after hatching they become BSF larvae transferred to enlargement media. BSF larvae are fed leftovers that enter the TPST, so that the enlargement medium is wet. Feeding leftovers from TPST is done every day. The rearing time for BSF larvae is for one life cycle. Maggot harvesting is carried out at the age of the larva about 2-3 weeks. The final products of organic waste bioconversion are BSF larvae (maggot), compost and POC. It was found that the BSF larvae (maggot) could reduce organic waste (municipal organic waste) by 47.75%. Where the ability of BSF larvae (maggot) to consume municipal organic waste is 26,1508 g waste / g maggot. The protein, crude fat and ash content in maggots which were given organic waste were 41.8%, 14.63% 9.12%, respectively.

Impacts of Killing Process on the Nutrient Content, Product Stability and In Vitro Digestibility of Black Soldier Fly (Hermetia illucens) Larvae Meals

The black soldier fly (BSF, Hermetia illucens) is considered a potential sustainable insect alternative source of protein for animal feed. The quality of a BSF meal is greatly influenced by the killing method and the purpose of this article is to compare the influences of different killing methods. BSFs at the 18-day-old prepupae stage were separated into six different killing methods with three replicates: 1. blending, 2. freezing, 3. CO2 treatment, 4. vacuum, 5. blanching and 6. CO2 plus blanching. After killing, BSF larvae meals were obtained by hot air oven drying and grinding. The chemical composition and in vitro digestibility calculated from sediments were not affected by the killing method, except that blending provided the worst BSF quality for all measured parameters (p < 0.05). The highest quality of BSF was obtained from the heat treatment procedures (blanching and the CO2 plus blanching methods), as they produced lower acidity after killing, total viable counts, browning reaction (enzymatic and non-enzymatic), darkness, moisture, fat acidity, protein and lipid oxidation during storage compared with other killing procedures (p < 0.05). Interestingly, the highest free amino acids in the supernatant after in vitro digestibility of BSF samples was observed with the CO2 plus blanching killing method (p < 0.05), whereas other parameters were similar to those obtained with blanching. The CO2 plus blanching method did not produce clearly different outcomes to blanching; therefore, the selection of one of these techniques over the other should depend on the regulations in each country.

Refining and Sulfurization of Oil from Black Soldier Fly and Its Application as Biodegradable Lubricant Additive

AbstractBlack soldier fly (BSF, Hermetia illucens), a high lipid containing insect, is considered as a promising candidate for bioconversion of restaurant waste in China. The fresh oil extracted from BSF larvae were refined by degumming, alkaline neutralization, and absorption decolorization. The gas chromatography–mass spectrometry analysis showed that 12:0, 16:0, 18:1, and 18:2 were the main fatty acids, which accounted for more than 80% of the refined BSF oil. Odorless sulfurized BSF oil was synthesized, and the tribological behaviors of the sulfurized BSF oil as a biodegradable additive in base oil 150 N and pure poly‐alpha‐olefin PAO4 were evaluated in comparison with the commonly used sulfurized lard by means of four‐ball friction and wear testers. The formation of CS bond in the vulcanized oil molecules was confirmed by FTIR analysis. Sulfurized BSF oil showed superior load‐carrying capacity and friction‐reducing ability compared with sulfurized lard.

Nutritional composition of black soldier fly larvae feeding on agro‐industrial by‐products

AbstractBlack soldier fly (BSF) larvae, Hermetia illucens L. (Diptera: Stratiomyidae), bio‐convert organic side streams into high‐quality biomass, the composition of which largely depends on the side stream used. In the present study, BSF larvae were reared on feed substrates composed of dried brewers’ spent grains, each supplemented with either water, waste brewer’s yeast, or a mixture of waste brewer’s yeast and cane molasses to obtain 12 different substrates: barley/water, barley/yeast, barley/yeast/molasses, malted barley/water, malted barley/yeast, malted barley/yeast/molasses, malted corn/water, malted corn/yeast, malted corn/yeast/molasses, sorghum‐barley/water, sorghum‐barley/yeast, and sorghum‐barley/yeast/molasses. The crude protein, fat, ash, and mineral contents of the BSF larvae fed each feed substrate were quantified by chemical analyses. The effect of substrate, supplementation, and their interaction on crude protein, fat, and ash contents of BSF larval body composition was significant. Calcium, phosphorus, and potassium were the most abundant macrominerals in the larvae and their concentrations differed significantly among substrates. These findings provide important information to support the use of BSF larval meal as potential new source of nutrient‐rich and sustainable animal feed ingredients to substitute expensive and scarce protein sources such as fishmeal and soya bean meal.

The Digestibility of Banana Peel and Testa coconut and Its Effects on the Growth and Mortality of Black Soldier Fly Larvae (Hermetia illucens) at Constant Feeding Rates

Organic waste produced by economic activities may create health, aesthetic, and economic problems. One of the approaches applied to solve this problem is the utilization of decomposer macrofauna to decompose the waste. One of the decomposers with great potential is Black Soldier Fly larvae (Hermetia illucens) that can consume various types of organic wastes and converts it into biomass with high protein and lipid content. In this study, banana peels and coconut testa had been fed to the larvae at 200 mg/larvae/day as the objects that represented organic wastes with low fiber content and high fiber content respectively. The purpose of this study was to observe the growth and efficiency of BSF larvae in decomposing those wastes. The analysis was conducted on some parameters such as the growth and consumption rate, the efficiency of conversion of digested (ECD), waste reduction index, and mortality rate. The results showed that BSF larvae THAT consumed banana peel had a higher final weight (58.24 mg), growth rate, and consumption rate while the mortality rate was lower than BSF larvae that consumed coconut testa. The ECD of the larvae group that consumed banana peel was higher than the larvae group that consumed coconut testa. The waste reduction index of banana peel was higher than coconut testa (1.5 and 1.4, respectively). The larvae that consumed coconut testa had a longer pupation period (9±1,75 days) compared to the larvae that consumed banana peel. Based on this result, it can be concluded that the fiber content of organic waste affected the decomposition rate and growth of BSF larvae.

Evaluation of Fertilizer Value of Residues Obtained after Processing Household Organic Waste with Black Soldier Fly Larvae (Hermetia illucens)

The residue generated by the black soldier fly (Hermetia illucens, BSF) during the processing of organic waste is considered a suitable crop fertilizer. However, no detailed studies have investigated the fertilizer value of the residue obtained from processing household organic waste. In this study, experimental household organic waste (EHOW) was processed by BSF at 200 mg of EHOW per head for 15 days at 27 °C. To evaluate the fertilizer value of the obtained BSF larvae production residue (BSFR), the chemical composition and microbiota were analyzed, and Komatsuna (Brassica rapa var. perviridis) cultivation tests were conducted. BSFR results demonstrated higher ammonium nitrogen and lower nitrate nitrogen, and the highest above-ground dry matter weight of Komatsuna. Although the relative abundance of Escherichia was low, the relative abundance of Xanthomonadaceae, which contains a genus that causes disease in plants, was high. Therefore, the presence of plant pathogens in the BSFR microbiota should be considered. Finally, the effects of BSFR on the external environment requires more detailed investigation.

The microbial safety of seaweed as a feed component for black soldier fly (Hermetia illucens) larvae

Farmed insects can offer an environmentally sustainable aquafeed or livestock feed ingredient. The value of black soldier fly (Hermetia illucens) (BSF) larvae could be improved by enrichment in omega-3 through the dietary inclusion of seaweed. However, the industry practice of drying seaweed at low temperatures to retain nutritional properties may benefit the survival of human pathogenic bacteria, particularly if the seaweed has been harvested from contaminated water. Here we have demonstrated that E. coli and E. coli O157:H7 died-off in seaweed dried at 50 °C, although both were detected in the dried powder following 72 h storage. V. parahaemolyticus fell below the level of detection in stored seaweed after drying at ≥ 50 °C, but L. monocytogenes remained detectable, and continued to grow in seaweed dried at ≤60 °C. Therefore, drying seaweed at low temperatures risks pathogen carry-over into insects destined for animal feed. BSF larvae reared on an artificially contaminated seaweed-supplemented diet also became contaminated by all four bacteria present in the supplement. Water quality at seaweed harvesting sites, seaweed desiccation, and insect rearing practices, represent critical points where development of regulatory standards could achieve targeted control of pathogenic hazards.

Dual oxidase Duox and Toll-like receptor 3 TLR3 in the Toll pathway suppress zoonotic pathogens through regulating the intestinal bacterial community homeostasis in Hermetia illucens L.

Black soldier fly (BSF; Hermetia illucens L.) larvae can convert fresh pig manure into protein and fat-rich biomass, which can then be used as aquafeed for select species. Currently, BSF is the only approved insect for such purposes in Canada, USA, and the European Union. Pig manure could serve as a feed substrate for BSF; however, it is contaminated with zoonotic pathogens (e.g., Staphylococcus aureus and Salmonella spp.). Fortunately, BSF larvae inhibit many of these zoonotic pathogens; however, the mechanisms employed are unclear. We employed RNAi, qRT-PCR, and Illumina MiSeq 16S rDNA high-throughput sequencing to examine the interaction between two immune genes (Duox in Duox-reactive oxygen species [ROS] immune system and TLR3 in the Toll signaling pathway) and select pathogens common in pig manure to decipher the mechanisms resulting in pathogen suppression. Results indicate Bsf Duox-TLR3 RNAi increased bacterial load but decreased relative abundance of Providencia and Dysgonomonas, which are thought to be commensals in the BSF larval gut. Bsf Duox-TLR3 RNAi also inactivated the NF-κB signaling pathway, downregulated the expression of antimicrobial peptides, and diminished inhibitory effects on zoonotic pathogen. The resulting dysbiosis stimulated an immune response by activating BsfDuox and promoting ROS, which regulated the composition and structure of the gut bacterial community. Thus, BsfDuox and BsfTLR3 are important factors in regulating these key gut microbes, while inhibiting target zoonotic pathogens.

Supplementation of black soldier fly (Hermetia illucens) on productivity and blood hematology

Black Soldier Fly larvae are natural antibiotics. The use of methanol extract in BSF larvae has a dual function, namely high protein content and high antibiotic content to kill Gram-negative bacteria. The biological structure of BSF larvae with antimicrobial peptide (AMP) acts as an inhibitor of pathogenic microorganisms, has a high lauric acid function as a natural antimicrobial and chitin, the polysaccharide plays a role in enhancing the immune response of the animal. This study aims to gain the best type of BSF protein from feed treatments containing live BSF, dry BSF, and BSF extract to increase blood productivity and hematology. The results of this study show that the best P3 treatment for the productivity of laying hens at the age of 18 to 26 weeks with the use of BSF extract since it contains the highest protein compared to other treatments. Productivity variables show the best P3 treatment, each egg weight 57.17 g bird-1, daily egg production 90.88%, egg mass 2408.16 g bird-1 and ration conversion 2.0. The mean hematology of the blood showed the highest P3 treatment, each erythrocyte 2.91±0.13 x 106 mm-3, leucocytes 17.69±3.68 x 103 mm-3, hematocrit 27.8±1.14%, hemoglobin 9.96±1.12%, MCV 95.77±4.41 fl, MCHC 35.99±5.21%, MCH 34.38±4.53 pg, lymphocytes 61.2 ± 4.86%, heterophile 31.2±2%, monocytes 5±0.62% and ratio H/L 0.51±0.04.

Substitutions of fish meal with larvae meal black soldier fly (Hermetia illucens) on the performance of female quail

BSF larvae meal contains protein which is very high so that it can be used as a protein source to replace fish meal. This study aims to determine how much the use of BSF larvae meal in substituting fish meal for consumption, weight gain and feed conversion in quails. This study used 200 7-day-old female quails that were kept for 6 weeks 42 days with an average initial weight of ± 23 grams. This study uses a completely randomized design (CRD), which consists of 4 treatments and 5 replications. Using experimental ration: P0 Ration without addition of BSF larvae meal; P1 = BSF larvae meal 3.183% (6.67% crude protein of fish meal); P2 = BSF larvae meal 6.370% (3.34% crude protein of fish meal); P3 = BSF larvae meal 9.565% (0% crude protein of fish meal). The parameters measured were the amount of consumption, weight gain, quail carried out every week during the study and feed conversion rate as an indicator of the efficiency of ration use. The results showed that ration consumption and weight gain and feed conversion did not significant affect (P> 0.05) meaning substitution of fish meal with BSF larvae meal until P3 could be carried out without negatively affecting the performance of quails.

Standardisation of quantitative resource conversion studies with black soldier fly larvae

Using larvae of the black soldier fly (Hermetia illucens; BSF) to convert low-value residual organic resources into high-value products like protein-rich animal feed ingredients and biofuel while managing organic waste has developed into a global industry. Considering the associated exponential increase in publications dealing with diet conversion efficiency by BSF larvae, it is timely to suggest procedures to arrive at an improved harmonization and reproducibility among studies. This means establishing protocols for describing the basic experiment design, fly colony origin, rearing procedures, reference and experimental feeding substrates, and sampling preparations including microbiota and chemical analyses. Such standardised protocols are instrumental to allow conversion efficiencies to be calculated. Some of these parameters are relatively easy to describe such as giving the origin and rearing conditions, while others are more challenging (e.g. description of microbe community). In this article we discuss and propose such procedures with the aim to arrive at standardisation of how future resource conversion studies with BSF larvae are conducted and how results are communicated.

Supplementation of Black Soldier Fly (Hermetia illucens) on Activity and Capacity Phagocytic Macrophage of Laying Hens

Black Soldier Fly are natural antibiotics. It is expected that the use of BSF larvae in poultry rations as an alternative source of conventional protein will contribute to improving the immune status and maintaining animal health, thereby reducing the use of antibiotic growth promoters (AGPs). This study aimed to evaluate the effect of the best type of BSF protein for determining the health status of laying hens based on the activity and capacity of macrophage phagocytosis on the non-protein A bacterium Staphyllococcus aureus. Results of this study indicated that the BSF extract (P3) has the highest phagocytic capacity value. This result proves that the BSF extract can induce macrophage cells to optimally process bacterial cells or foreign phagocyte particles. The highest average activity value, and phagocytic capacity of peritoneal macrophages was resulted from BSF extract (P3), respectively was 91.34 ± 0.38% and 22.84 macrophage-1 bacteria.

Modeling the Growth of Black Soldier Fly Hermetia illucens (Diptera: Stratiomyidae): An Approach to Evaluate Diet Quality.

The black soldier fly (BSF, Hermetia illucens (Linnaeus), Diptera: Stratiomyidae) is an edible insect widely reared by using various types of organic wastes as its diet. Developing tools to evaluate the growth performance of BSF is crucial for improving its production. In this study, we monitored the daily growth of BSF larvae reared with 10 diets with diverse nutritional quality and compared 7 growth models for quantifying growth performance. We found that BSF generally exhibited an S-shaped growth curve and that the Richards model was the best-fitting growth model. We estimated asymptotic growth limit (W∞) and constructed a time-series curve of absolute growth rate (AGR) by using the Richards model. We used Gaussian functions to estimate AGR parameters (maximum AGR, m; timing of maximum AGR, T; the deviation of AGR, d) to evaluate whether these parameters adequately quantify the growth performance of BSF. Correlation analysis showed that the AGR parameters were mostly independent of W∞ but were correlated with the duration of the larval stage, indicating that W∞ and the AGR parameters respond to different dietary factors. The results of pairwise comparisons showed that the diets rich in carbohydrates and protein had high W∞, m, and d, and early T. The diets with low levels of carbohydrates had low W∞, and the diets with low levels of protein had low m and d and delayed T. We conclude that the parameters estimated by the Richards model could be effective indices for evaluating the growth performance of BSF and their diet quality.

Impact of the Feeding With the Black Soldier Fly (Hermetia Illucens) on Egg Physical Quality, Egg Chemical Quality and Lipid Metabolism of Laying Hens

BSF larvae have a high growth rate and optimal feed conversion and can be well utilized of various types of material as a food source including decomposed organic matter. BSF larvae have amino acid compositions that resemble the amino acid composition of soybean meal or fish meal so that they can function as (antibiotic growth promoters-AGP). AGP aims to maintain the health of the digestive tract of poultry so that feed conversion becomes more efficient and growth performance can be optimal. This study aims to obtain the best type of BSF protein to improve egg quality physically and chemically and the effect of BSF on lipid metabolism. The results of this study indicated that treatment of T3 was the best physically egg quality and chemically egg quality of-laying hens with the use of BSF because it contained protein extract was quite high compared to other treatments. The results of the analysis of blood lipid metabolism that the treatment of live BSF (T1) has the lowest average value, this proved that live BSF was able to reduce the fat content in the blood of laying hens.

Integrated waste management through symbiotic culture: A holistic approach

Unsegregated heterogeneous mass of solid waste is a potential threat towards the safe processing and disposal of municipal solid waste (MSW), which is cumbersome and ancillary, elevates the expenditure. The absence of any pertinent technique, to handle these bulky mass in an eco-friendly way, makes it even more hectic for the MSW processing plants. But, this substantial mass constitutes a huge potential, which can be valorized through the larval digestion of a symbiotic macro culture, to create new economic niches for small-scale entrepreneurship in developing and under-developed nations. The present study incorporates a symbiotic waste reduction system with the efficient functionalism of micro and macro conversion agents which is attributed to the synthesis of waste derived co-products from the same platform. This study is solely a novel approach, which is capable of addressing a wide fraction of solid waste, domestic sewage, and bio-leachate and converts them into value-added co-products within the magnificent period of time. Multiple experiments have been conducted under the controlled feeding environment with an established symbiosis between the BSF larvae, Wax Moth larvae (Galleria melonella), and effective microorganism culture. An initiation period of 5 days was allocated for the macro conversion agents and during the tenure, the larvae were supplied with synthetic diet to accelerate the body growth index. The entire experiments have been conducted in a newly formulated novel bioreactor namely, Integral Larval Grub Composting Reactor which is capable of performing automated pupal segregation and address solid and liquid waste under a single platform. The system enforces the zero liquid discharge concepts and also helps in an indirect suppression of house fly growth. The novel approach only required 14-16 days in order to convert the solid waste into nutrient-rich manure, having significantly high-quality component. The operation has been executed in a number of batch processes and each process valorized a quantity of approx 30 kg. The targeted larval period has been prolonged by means of maintaining higher moisture content with the help of sewage supply and once the maturation period arrives the further addition of moisture has been restricted for 3 days. Overall it’s an ultimate and wholesome biological waste management technique, which is capable of standing solely to strengthen the sustainable waste management strategy.

Possibilities for the Use of Black Soldier Fly (Hermetia illucens) in Poultry Breeding as a Feed and Waste Utilization

Black soldier fly (BSF, Hermetia illucens), may be a reliable source of animal protein, fats, calcium, lysine, etc. This makes it possible the products obtained by this insect to become substitutes (partly orentirely) of the soybean and fish meal. First of all, however, different issues should be solved to react on the future increase in the demands for such kind of products as feed. This means to dramatically change the technology of manufacturing and to introduce regulatory legislative frameworks in which this industry to be developed, guaranteeing its safety for the customers. This is necessary despite the fact that in BSF larvae such preconditions due to the biological and cycle characteristics are not observed, as naturally it excludes the transfer of pathogens from the bed because of the disconnection of the composted matter at the prepupae stage. This is a result of natural migration, during which the larvae are self-cleaning mechanically and chemically, while emptying their gastrointestinal tract. Additional reliability is also assured by the fact that the adult insect does not feed, does not lay eggs directly in the compost and is not a carrier of infectious and parasitic diseases. Since it is a natural resident of the poultry manure, it can also be successfully used for its processing, on the one hand reducing the volume, and on the other decreasing the concentration of the pathogenic microflora in it, which naturally leads to its decontamination. In terms of origin, some drawbacks have also been identified, however, the effect of which can easily be neutralized by developing the systems used and regulating the composition of the food substrate.