Bryophyte Samples Research Articles

Moss-associated diatoms from Malaysian montane cloud forest with a description of two new species

Although tropical montane cloud forests (TMCFs) occupy less than half of a percent of the Earth’s land surface, they are the most species-rich and species-dense ecosystems worldwide. Despite numerous studies on various organisms in this environment, diatoms remain a group that is practically unknown. Our work focused on samples of terrestrial bryophytes collected from various substrata at a single site in the Malaysian cloud forest. Despite originating from only one site, the samples contained a high diversity of diatom species and represented a wide range in pH (3.6–6.3). In our study, 62 diatom taxa were observed (8–24 per sample), of which 19 could not be identified with certainty, and were referred to established taxa of similar morphology (as cf. or aff.). Among these however, two species are described as new on the basis of their unique valve morphology: Geissleria bryophila sp. nov. and Eunotia brinchangiana sp. nov.

Cryptogamic Biomass in Pannonic Acidic Sand Steppes Subject to Changing Land-Use.

Cryptogams, often neglected in vegetation dynamics studies, compose a large part of biomass and contribute to the biodiversity of sandy grasslands. Since the work of Verseghy (1970s), their productivity has not been analyzed in Hungary. We studied the lichen and bryophyte dynamics (hereinafter called cryptogams) at two Eastern Hungarian dry sandy grassland sites. The sites of Corynephorus canescens and of Festuca vaginata dominance, respectively, belonging to the community Festuco vaginatae-Corynephoretum have been monitored. We aimed at (1) quantifying the diversity and biomass of the cryptogamic communities; (2) exploring the cryptogamic response to management changes; and (3) studying the effect of experimental management (fencing) on the cryptogamic assemblages. The sites have been compared in 2013 and 2018, respectively. Forty microplots per site per management have been analyzed in both years. Samples of lichens and bryophytes were hand-sorted, dried and then measured. Fencing has led to increased biomass of cryptogams within a few years. Lichens in general benefited comparatively more from exclosure than bryophytes. The increase in lichen biomass (especially that of Cladonia rangiformis) is clearly due to the over 10-year absence of grazing. The only lichen favored by moderate grazing is the legally protected C. magyarica. Short spells of low-intensity grazing can promote the species richness of cryptogams in the community.

Diversity of epiphytic bryophytes in Medan City Parks, North Sumatra, Indonesia and its potential as lead (Pb) bio-accumulators

Abstract. Siregar ES, Pasaribu N, Lubis AF. 2023. Diversity of epiphytic bryophytes in Medan City Parks, North Sumatra, Indonesia and its potential as lead (Pb) bio-accumulators. Biodiversitas 24: 3214-3221. The information on the diversity of epiphytic bryophytes in the metropolitan area, particularly Medan City, North Sumatra, Indonesia has been limited. Therefore, this study aimed to conduct an inventory of the diversity of epiphytic bryophytes in Medan City parks as well as to assess their potential as lead (Pb) bioaccumulators. Three sampling sites, namely Ahmad Yani Park, Beringin Park, and Medan Zoo, were selected based on their pollution levels from high to low, respectively. Five plots with dimensions of 25×25 m were placed at each site and five representative host trees were selected in each plot. Lead (Pb) bioaccumulation of bryophyte samples is determined using the atomic absorption spectroscopy (AAS) method at the Plant Research Center Laboratory, Medan. The Pb-bioaccumulation capacity of each species from different sites was compared through two-way ANOVA. The results showed eight species, namely four liverworts (Marchantiophyta): Cololejeunea lanciloba Steph., Lejeunea cocoes Mitt., Lopholejeunea eulopha (Taylor) Schiffn., and Lopholejeunea subfusca (Nees) Schiffn., as well as 4 Mosses (Bryophyta): Calymperes tenerum C.Müller, Meiothecium microcarpum Mitten, Octoblepharum albidum Hedwig and Vesicularia dubyana Brotherus. Vesicularia dubyana was the dominant species at Beringin Park and Medan Zoo, while C. tenerum was dominant at Ahmad Yani Park. The biodiversity of epiphytic bryophytes in this study was categorized as moderate level (1?H’?3). The highest Important Values Index (IVI) of epiphytic bryophytes in this study was obtained for V. dubyana at 131.80. The highest Pb bioaccumulation was observed in O. albidum at 87.5 mg/kg, followed by V. dubyana at 66.6 mg/kg. This study provided information on the ecological contribution of epiphytic bryophytes as Pb bioaccumulators in urban areas. Vesicularia dubyana and O. albidum can be recommended as biomonitoring agents in the future.

Bryomonitoring of atmospheric elements in Sphagnum sp. commonly growing bryophyte in the Indian Himalayan region of Uttarakhand

For a range of metals originating from both natural and anthropogenic sources, bryophytes are helpful biological markers of environmental contamination. This research focuses on recent changes in air quality and uses a passive biomonitoring technique to estimate atmospheric metal deposition and its seasonal trend in Sphagnum sp., as well as its substrate, from Nainital, Bhimtal, and Mangoli of Uttarakhand, India. Bryophyte samples from the summer, winter and monsoon seasons were collected from equally spaced locations. Due to the large volume of travel, a high metal concentration was discovered in Nainital. The metal deposition loads were reported in the following order: Zn > Cu > Pb > Cd. Quantitative analysis of these elements in vegetative/plants and substrate/soil showed an increase in metallic content during summer. The results indicate this moss may serve as an important tool in estimation of both aerial pollution and mineral enrichment in soil. Such research is vital since development is frequently accompanied by unfavourable changes in air quality and negative impacts of air pollution on human health, agricultural production, and natural ecosystems that need to be monitored and mitigated.

Diversity of bryophilous fungi in desiccation-tolerance bryophyte plants

Bryophilous fungi associated with two bryophyte species in the region of delta in Tamil Nadu that is, the mosses of Polytrichum commune and Bryum capillare (Ptychostomum capillare) mosses were studied during the winter season. These bryophytes were Desiccation-Tolerance Bryophytes plant (DTB), the ability to lose virtually all free intracellular water and then recover normal function upon rehydration is one of the most remarkable features of bryophytes. Our aims were to identify bryophilous fungi/ endophytic fungi using morphological taxonomy, to explore richness and estimate species frequency in two bryophytes, Polytrichum and Bryum in delta region of Tamil Nadu. We collected two bryophyte samples at old building wall, bricks localities on delta region. We thus identified 32 endophytic fungi species were isolated from these bryophyte plants tissues. In total, 32 endophytic fungal strains belonging to Ascomycetes (04), Coeleomycetes (02), Hyphomycetes (20), sterile forms (02), Yeast like strains (02) and Zygomycetes (02) taxa were obtained. Thus species RPO and diversity index suggest that our study still underestimate endophytes diversity because it does not provide complete samples. These results recommend the presence of a diverse range of fungal species even in a very limited area, and those bryophytes fungal diversity in this Desiccation-Tolerance environment. To our knowledge, this is the first report of endophytic fungi from DT Bryophytes in tropical region.

Bryophyte species differ widely in their growth and N2-fixation responses to temperature

Bryophytes are abundant in tundra ecosystems, where they affect carbon and nitrogen cycling through primary production and associations with N2-fixing bacteria. Bryophyte responses to climate warming are inconclusive, likely because species-specific responses are poorly understood. Here we investigated how warming affects the growth and nitrogenase activity of 10 tundra bryophyte species in two tundra landscapes. Collected bryophyte samples were grown in temperature-controlled growth chambers for 12 weeks at five temperatures from 3 to 18 °C. We measured growth, N concentration, δ15N, and δ13C after 3 months and nitrogenase activity after 5 and 12 weeks. Bryophyte growth and associated nitrogenase activity generally increased with temperature, but species differed in their optima. Bryophyte N concentration and δ15N indicated that, for some species, increased N2-fixation could not compensate for growth-induced N limitation. High landscape coverage and large positive warming effects on feather mosses and Sphagnum species highlight their competitive advantages, confirm earlier field observations, and contribute to the mechanistic understanding of differential bryophyte growth in response to warming. We suggest that indirect effects of climate change, such as surface drying and shrub expansion, are likely main threats to slow-growing bryophytes across the Arctic, with consequences for biodiversity and C balance.

Taxonomic and biogeographical analysis of diatom assemblages from historic bryophyte samples from Campbell Island (sub-Antarctic)

Background and aims – The past two decades, the non-marine diatom flora in the sub-Aantarctic region has been intensively revised. Historic collections provide excellent tools for answering taxonomic, community-related, and biogeographical questions. This study analysed the moss-inhabiting diatom flora from sub-Antarctic Campbell Island in samples collected in 1969–1970 and retrieved from the British Antarctic Survey herbarium (Cambridge, UK). With this study we attempt to expand our, till now sparse, knowledge on the moss-inhabiting diatom flora in the southern Pacific Ocean.Material and methods – In total, the diatom composition in 32 moss samples has been analysed using Light Microscopy (LM) and Scanning Electron Microscopy (SEM). A biogeographical analysis and community analysis of the Campbell Island diatom flora were conducted.Key results – Analysis of the Campbell Island diatom flora revealed the presence of 141 taxa belonging to 45 genera. The species composition in the dominant genera Planothidium, Humidophila, and Psammothidium, the presence of Epithemia, Rhopalodia, Cocconeis, uncommon in the sub-Antarctic region, and many unidentified taxa point to the uniqueness of the Campbell Island diatom flora. The biogeographical analysis showed an overall low similarity with the other sub-Antarctic and the Maritime Antarctic islands. Four different diatom assemblages were distinguished following the community analysis. Moisture level and habitat type seem to be the main factors shaping the Campbell Island diatom assemblages.Conclusion – The results of the Campbell Island diatom analysis highlight the importance of historic herbarium material. Since the examined flora is largely composed of unknown (presumably new) species, it is vital to analyse additional (historic) samples to complete the assessment of the moss diatom assemblages from Campbell Island.

New species and records of tardigrades from a biological repository collection from the Sierra Nevada de Santa Marta, Colombia.

The Sierra Nevada de Santa Marta (SNSM) is a mountain range in northern Colombia, recognized for its high biological richness. To date, the existence of 27 species of tardigrades is known for this particular region. Morphological and morphometric analysis of 51 specimens, found in 16 samples of bryophytes and lichens collected at the SNSM, and deposited in the tardigrade collection of the "Centro de Colecciones Científicas de la Universidad del Magdalena" between 2011 and 2016 was carried out. The species Mixibius gibbosus sp. nov. is described based on the following main traits: presence of gibbosities (up to date never reported for any species of the genus Mixibius), isodiametric tubercles and relevant morphometric differences with respect to the most similar congeneric species. Additionally, the species Diphascon pingue pingue sensu lato, Hypsibius cf. allisoni and Adropion onorei are, for the first time, recorded for Colombia and the latter is recorded for the first time out of Ecuador. All records are new for the SNSM.

Small areas and small plants: Updates on Antarctic bryophytes

Snow Island is part of the South Shetland Archipelago in Antarctica. Most of its surface is permanently covered by snow, yet it has an important paleobotanical site. There are no protected areas on the island and no recent data regarding its vegetation. This study aimed to collect and identify fresh samples of bryophytes from President Head Peninsula of Snow Island. Samples were collected during the summers of 2015 and 2018. Among the 24 bryophyte species identified in this work, 19 are new occurrences for Snow Island, bringing the total known for the island to 29 species. The most diverse family is Pottiaceae, with four species of two genera, followed by Bryaceae and Polytrichaceae, with three species each. The results show that the diversity of mosses on Snow Island is greater than previously reported. We here demonstrated the region's importance and the 190 % increase in Snow Island species number. This study also updated the Antarctic distribution of some species.

The Bryophyte Flora of Doğu Küre Mountains

Bu çalışma, Türkiye’nin kuzeyinde Kastamonu, Sinop ve Samsun illeri sınırlarında uzanan Doğu Küre Dağları’nın briyofit çeşitliliği hakkında bilgi sunmaktadır. Araştırma alanına 2014-2016 yılları arasında yapılan 7 arazi çalışmasında 244 lokaliteden 1600 briyofit örneği toplanmıştır. Toplanan örneklerin teşhis edilmesi sonucunda 310 tür/türaltı briyofit taksonu (2 boynuzotu, 58 ciğerotu, 250 karayosunu) tespit edilmiştir. Bu taksonlardan 4 tanesi A2 karesi, 44 tanesi ise A3 karesinden burada ilk defa rapor edilmektedir.

In vitro medicinal potentials of Bryum capillare, a moss sample, from Turkey.

In this study was conducted the in vitro antimicrobial, antibiofilm, antioxidant, antigenotoxic and anticancer activities investigations on the moss Bryum capillare Hedw (BC). Antimicrobial and antibiofilm activity were tested by MIC and microplate biofilm methods on antibiotic resistant bacteria. While the antioxidant activity of the extract was evaluated by DPPH, metal chelating, plasma lipid peroxidation and total phenolic content, the antigenotoxicity and cytotoxicity were established by Comet test and the WST-1 Cell proliferation assay kit respectively. The MIC values were found to be≥125µg.mL-1 and a biofilm inhibition of 3-5% against only S. epidermidis was observed. Total phenolic compounds were determined as 23.26mg/g. The results of DPPH assay, chelating and plasma lipid peroxidation activity were found to be 15%, 3% and 4% respectively. The extract was observed to decrease the affect of H2O2 that cause DNA damage. The BC was also determined 60±5% anticancer activity against SKBR 3 and 76±5% anticancer activity against HeLa cells, where this concentration had only 18±5% cytotoxicity against MCF-12A cells. Also, these results have indicated the potential of Bryum capillare for the first time in novel natural compounds search.

Assessment of urban environmental quality through the measurement of lead in bryophytes: case study in a medium-sized city.

The use of bryophytes as an indicator of environmental quality has been addressed on numerous occasions and in different places of the world under a variety of conditions. However, in Latin America their use is still limited. In the study described here, the presence and distribution of the bioaccumulation of lead in bryophytes has been evaluated in both contaminated and uncontaminated sites in Villavicencio (Colombia) and its surroundings. Villavicencio is a medium-sized city that is similar in size to many other cities in Latin America. A total of 52 samples of bryophytes were collected, of which 43 belong to points distributed in urban areas of the city (residential, commercial, highway), and the remaining nine are from uncontaminated areas (reference) taken in the surroundings of the city. The samples were treated with acid (nitric and hydrochloric) and subsequently measured by atomic absorption spectrometry. It was found that Pb concentrations in the commercial sector are between 1 and 6 times higher than in the residential and highway sectors. Spatial distribution maps of lead over the sampled territory were created using Arc-GIS 10.1. It is noteworthy that the values obtained are higher than those found in European cities.

Epiphytic bryophyte diversity and range distributions along an elevational gradient in Marojejy, Madagascar

AbstractDescribing spatial variation in species richness and understanding its links to ecological mechanisms are complementary approaches for explaining geographical patterns of richness. The study of elevational gradients holds enormous potential for understanding the factors underlying global diversity. This paper investigates the pattern of species richness and range‐size distribution of epiphytic bryophytes along an elevational gradient in Marojejy National Park, northeast Madagascar. The main objectives are to describe bryophyte species composition and endemism in Marojejy National Park, to describe the species richness and distribution patterns of epiphytic bryophytes along an elevational gradient from 250 m to 2050 m and to evaluate the explanatory value of environmental variables for the observed patterns. Bryophyte samples were collected following a nested design with four hierarchical levels: elevational belts, plots, quadrats, and microplots. In total, 254 epiphytic bryophyte species were recorded, comprising 157 liverworts and 97 mosses. Twenty‐three of these are endemic to Madagascar. Species richness exhibits a hump‐shaped pattern along the elevational gradient, peaking at 1,250 m. Eighty‐seven percent of the total recorded species have a range distribution lower than 1,000 m, at which point 36% are restricted to these single elevations. Our results suggest that mean temperature, relative humidity, and vapor pressure deficit play important roles in shaping the richness pattern observed in this study. While the liverwort richness pattern did not correlate to vapor pressure deficit and responded only weakly to relative humidity, the richness pattern shown by mosses correlates well with mean temperature, relative humidity, and vapor pressure deficit.

Where are they hiding? Testing the body snatchers hypothesis in pyrophilous fungi

Abstract Pyrophilous fungi produce sporocarps after a fire but little is known about their ecology prior to or after a fire event. Recently, the body snatchers hypothesis was proposed that suggests some post-fire fungi form endophytic and/or endolichenic relationships with plants and lichens. To test the body snatchers hypothesis, bryophyte, lichen, club moss, and soil samples were collected from unburned and mixed-intensity burned areas 1–2 y after a 2016 wildfire in the Great Smoky Mountains National Park, and from unburned areas in four states outside the park. Samples were examined for the presence of pyrophilous fungi occurring as endophytes or in lichens using culture-dependent and culture-independent techniques. Culture-dependent methods isolated Pholiota highlandensis, a known pyrophilous fungus, from five bryophyte samples. Culture-independent methods identified 22 pyrophilous taxa from bryophyte, club moss, lichen, and soil samples across a range of geographical localities. The ‘body snatchers’ hypothesis is supported since many bryophyte, lichen, and club moss samples contained pyrophilous taxa suggesting that these fungi occur as endophytes and/or endolichenic fungi until a fire event triggers them to produce sporocarps.

Responses of soil mite communities (Acari: Oribatida, Mesostigmata) to elemental composition of mosses and pine needles and long-term air pollution in Scots pine (Pinus sylvestris L.) stands.

Air pollution is an important threat to biodiversity via deposition of high amounts of heavy metals or nutrients (macroelements). In forest ecosystems contamination can be found in plant tissues and the soil environment including soil mesofauna. However, there is little information on how it influences soil mesofauna. Hence, the aim of the study was to evaluate the reaction of soil mites (Acari: Oribatida, Mesostigmata) to long-term air pollution in mature pine (Pinus sylvestris L.) forests in southwestern Poland. The study was conducted in late autumn between October 2008 and 2010 in eight 5000 m2 plots, each within a Scots pine stand. Concentrations of macroelements (C, N, S, Ca, Mg) and heavy metals (Cu, Cr, Cd, Ni, Pb, Zn) were measured in 40 samples of pine needles and 36 bryophyte samples. In total, 360 soil samples were collected for the soil mesofauna analysis. Results of the study include correlations between the sample plot, the year and the soil mite abundance. Among the macroelements analyzed, calcium affected the abundance of mite species the most. Soil mite communities from different forests were dominated by the same species, despite the fact that we found in total 150 mite species, among which there were 106 species of oribatid mites and 44 species of mesostigmatid mites. It seems that, among the elements analyzed, calcium plays the most important, positive role for mite communities. Magnesium had a positive effect on abundance of both mite groups, while nitrogen had a negative effect on diversity of oribatid and mesostigmatid mite communities. Our study indicated that oribatid and mesostigmatid mite communities are stable in areas of long-term contamination, as we did not observe distinct changes in structure and diversity of soil mite assemblages along the pollution gradient.

Influence of Nano-ZnO Exposure to Plants on L-Ascorbic Acid Levels: Indication of Nanoparticle-Induced Oxidative Stress.

With the continuously growing production of nanomaterials, their presence in the environment increases. The effects of nanoparticle exposure on plants are yet not fully understood. This study aimed to provide new insights into the stress induced by the exposure of plants to nanoparticles via the determination of the content of L-ascorbic acid, known antioxidant, in the samples of bryophyte species Pleurozium schreberi (Brid.) Mitt. Bryophyte samples, collected from a pristine environment, were exposed to 0.1 g·L-1 suspensions of nano-ZnO for five weeks. Prior to the exposure, the samples were subjected to the combination of treatments-washing or not washing as well as drying or irrigation. In order to assess the level of oxidative stress caused by the exposure to the ZnO nanoparticles under various pre-treatment conditions, the content of the L-ascorbic acid in the samples was determined by titration with dichlorophenolindophenol. Effect of both nano-ZnO exposure and pre-treatment was observed; nano-ZnO exposure significantly reduced the content of L-ascorbic acid in the bryophyte samples indicating it being consumed in the repair processes following the exposure-related physiological stress.

Taxonomy and richness of yeasts associated with angiosperms, bryophytes, and meltwater biofilms collected in the Antarctic Peninsula.

The interest in the diversity of yeasts in the Antarctic environment has increased in recent years, mainly because Antarctic microbiology is a recent science, and little is known about the biodiversity and genetic resources of the microorganisms that inhabit this ecosystem. This study aimed to determine the diversity of epiphytic yeasts in samples of Deschampsia antarctica, Colobanthus quitensis, and bryophytes, as well yeasts present in biofilms collected from Antarctic meltwater. Samples were collected in the summer of 2014 and 2015 during expeditions organized by the Brazilian Antarctic Program. A total of 310 yeasts were isolated, and 34 species were identified by sequencing the D1/D2 domains of the rDNA region belonging to 18 genera. The species Vishniacozyma victoriae and Mrakia gelida were the most abundant. Dioszegia antarctica and Leucosporidium creatinivorum were found only in plant substrates. Most psychrophilic yeasts were isolated from biofilms, including Glaciozyma antarctica, Glaciozyma martinii, Mrakia gelida, Mrakia frigida, Mrakia robertii, Phenoliferia glacialis, and Phenoliferia psychrophenolica, suggesting that the substrates examined in this study represented an interesting habitat for the isolation and characterization of epiphytic and non-epiphytic yeasts that colonize the Antarctic region.

Ice Nucleation Active bacteria in Mount Lawu forest, Indonesia: 3. Isolation and estimation of bacterial populations on bryophyte

Abstract. Latifah NH, Susilowati A, Suratman. 2018. Ice Nucleation Active bacteria in Mount Lawu forest, Indonesia: 3. Isolation and estimation of bacterial populations on bryophyte. Asian J For 2: 67-75. This study aimed to isolate and estimate the Ice Nucleation Active (INA) bacteria population on bryophyte from the trekking route of Cemoro Sewu, Mount Lawu, Indonesia. Bryophyte samples were taken at three different altitudes, i.e., 2,000, 2,300, and 2,500 m above sea level (asl). Isolation of INA bacteria was carried out by the method of spread plate on King's B and nutrient agar plus 2.5% glycerol (NAG) medium. Bryophyte was identified by referring to the literature of Sutama (1995), Hasan and Ariyanti (2004), Ignatova and Samkova (2006), and Damayanti (2006). Ice nucleation activity was determined by the multiple-tube test method. The bacterial suspension tube was put into the circulating alcohol bath at a temperature of -10°C for 5 minutes. The number of INA bacteria on the bryophyte was estimated with the nucleation multiple tube method. The number (cell/g) of INA bacteria from the fresh weight of bryophyte was estimated based on the Most Probable Number (MPN) tables according to the formula of Thomas's 333 series. INA bacteria were identified through morphological and biochemical characters. Independent Sample T-Test analyzed the population of INA bacteria on terrestrial and epiphytes bryophytes with a significance of 5%. The results showed that 7 INA bacteria were isolated from the bryophyte Campylopus umbellatus (Arn.) Paris (Leucobryaceae, Musci). The population of INA bacteria in terrestrial bryophytes at each altitude were greater with 346 cell/g, 86 cell/g, and 396 cell/g, respectively, than that in epiphytes bryophyte with 50 cell/g, 50 cell/g, 176 cell/g, respectively.

Antimicrobial activity of Isothecium alopecuroides and potential effect of some climate elements on the activity of this bryophyte sample

Aim of study: This study aims to present how some climate elements can dramatically affect the antimicrobial activity of a plant by using Isothecium alopecuroides (Lam. ex Dubois) Isov., a bryophyte, as a plant sample to put forward some important environmental factors.Material and Methods: Two bryophyte samples, which were collected from two different locations, namely Aydın and Muğla. Chloroform, benzene, diethyl ether, ethyl alcohol, methyl alcohol, ethyl acetate, sdH2O and 0.5 M Tris-HCl buffer (pH: 8.0) were used as extraction solvents. The antimicrobial activity were tested against Bacillus subtilis, Candida albicans, Escherichia coli, Listeria monocytogenes, Salmonella enteretidis, Shigella flexneri, Staphylococcus aureus and Yersinia enterocolitica by disk diffusion, minimum inhibitory concentration test and minimum bactericidal/fungicial concentration tests. Meteorological data were obtained from the Turkish State Meteorological Service.Main results: As a result, it was observed that the bryophyte sample collected from Aydın was only active against B. subtilis with only its benzene extract, but the sample collected from Muğla was active against B. subtilis, S. aureus, E. coli and C. albicans with its different extracts at different ranges. Some climate elements were also found to be important in the activity of samples.Research highlights: It is concluded that in any study based on secondary metabolites, the time and the environmental conditions, where the plant samples are collected should be taken into account.

History of formation and modern structure of the bryolological herbarium of the State Natural History Museum of the NAS of Ukraine

The history of formation of the bryological herbaria of the State Natural History Museum of the National Academy of Sciences of Ukraine is considered. Many collectors and scientists-botanists took part in the formation of the main scientific fund of the bryological herbaria, among them A. Lazarenko, K. Ulychna, V. Melnichuk, M. Slobodian and others. The article contains a list of samples of bryophytes, which are included in the Red Book of Ukraine. Rare samples (including doublets and exsiccates) are described from territories of other countries, as well as specimens dating to the end of the 19th century.