Brush Cytology In Diagnosis Research Articles

Role of Bronchial Brush Cytology in Diagnosis of Bronchopulmonary Lesions

Bronchial carcinoma is currently the most common cause of cancer-related mortality worldwide. The increasing incidence could be due to increased smoking habits, change in lifestyles, increased environmental pollution, and the availability of different modalities to detect lung cancer. Fibreoptic bronchoscopy was introduced in 1968 to evaluate bronchopulmonary lesions. Since then, various methods for obtaining good specimens have become available apart from sputum. A higher positive rate was obtained from specimens collected by fiberoptic bronchoscope. We aimed to assess the sensitivity and specificity of brushings compared with the biopsy of lung lesions. The study was done on 100 patients, of which bronchial brush cytology was done in all 100 cases, and endobronchial biopsy was carried out in 20 cases. This study showed sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of brushing to be 87.5%, 75%, 85%, 93.13%, and 60%, respectively.
 TAJ 2022; 35: No-1: 107-115

Role of Brush Cytology in Diagnosis of Bronchial Carcinoma: Experience in Rajshahi Medical College Hospital

This was a descriptive study conducted in Rajshahi Medical College Hospital (RMCH), Rajshahi and the study period was from June 2017 to June 2019. A total number of 300 patients (48 females and 252 males) were enrolled who underwent elective fiber optic bronchoscopy with brush cytology for the diagnosis of bronchial carcinoma. Our aim was to identify the role of brush cytology in diagnosis of bronchial carcinoma. Most of the cases (84%) were male. Majority 111(37.00%) of the patients were in age group 60-72 years; the mean±SD age was (58.70±13.11). Most of the study participants were farmer 134(44.67%). 182(66.67%) were smoker and 118(39.33%) were non-smoker. For smokers, pack year history was <10 pack in 78(42.85%), 10-20 pack 66(36.26%) and >20 pack 38(20.87%) of cases. Squamous cell carcinoma was the most common type of bronchial carcinoma; found in 95 (31.66%) cases followed by adenocarcinoma 50 (16.66%), large cell carcinoma 43 (14.33%), small cell carcinoma 25 (8.33%), others 87 (29%) This study revealed that the bronchial brush cytology was minimally invasive, well enduring technique with high sensitivity in diagnosis of central lesion of bronchial carcinoma.
 TAJ 2019; 32(2): 1-6

The diagnostic accuracy of colonoscopic brush cytology in diagnosis of colorectal malignancies: A study of 49 patients.

To find the spectrum and frequency of lower gastrointestinal malignancies and diagnostic accuracy of colonoscopic brush cytology in their diagnosis. This was a prospective study carried out on 49 patients in the Department of Pathology over 1 year. Brushing material was smeared directly onto at least two clean glass slides. The air dried smears were stained with May Grunwald Giemsa stain. The endoscopic biopsies were examined grossly and were fixed in 10% formalin, processed and stained with Hematoxylin and Eosin stain, respectively. Special stains were used wherever required. The study was done on 49 patients presented with colorectal and anal lesions. Age of the patients ranged from 17 to 72 years with male to female ratio being 1.57:1. On statistical analysis, the sensitivity of colonoscopic brush cytology was found to be 85.71% and specificity 61.53%. The accuracy came out to be 79.16%. Brush cytology is a reliable, safe, inexpensive, and rapid method of diagnosing gastrointestinal lesions. Since brushing is a relatively noninvasive procedure, routine use of brushings of colonoscopically visible lesions should be done, in addition, to biopsy to increase the diagnostic yield.

Diagnostic value of intraductal ultrasonography and brush cytology on differentiating malignant and benign biliary stricture

Objective To investigate the diagnostic value of intraductal ultrasonography (IDUS) with or without brush cytology on distinguishing malignant from benign biliary stricture. Methods The data of patients with biliary stricture, who underwent endoscopic retrograde cholangiography(ERC) and IDUS in Drum Tower Hospital from September 2007 to October 2015 were retrospectively analyzed. Features of IDUS images and results of brush cytology were compared with postoperative histological results to evaluate the diagnostic yield of IDUS and brush cytology on differential diagnosis of malignant biliary stricture. Results A total of 230 patients with biliary stricture underwent ERC and IDUS, and 206 patients meanwhile received brush cytology, including 84 cases of malignant biliary stricture. Of the 230 patients, 87 cases underwent surgery, and 75 revealed malignant results, including 65 cases of cholangiocarcinoma, 8 cases of pancreatic adenocarcinoma, and 2 cases of periampullary adenocarcinoma. Using postoperative pathologic results as the gold standard, 72 malignant and 9 benign biliary strictures were correctly diagnosed by IDUS. The sensitivity, specificity, positive predictive value, and negative predictive value of IDUS for diagnosis of malignant biliary stricture was 96.0%, 75.0%, 96.0%, and 75.0%, respectively. Sixty-five patients underwent IDUS with brush cytology during ERC before surgery, and 56 out of 65 showed malignant results. Thirty-one malignant and 9 benign biliary stricture were correctly diagnosed by brush cytology. The sensitivity, specificity, positive predict value, and negative predict value of brush cytology for diagnosis of malignant biliary stricture was 55.4%, 100%, 100%, and 26.5%, respectively. If brush cytology combined with IDUS to diagnose malignant biliary stricture, the sensitivity rose up to 94.6%, while the specificity went down to 77.8%, and the positive and negative predictive value was 96.4% and 70.0%, respectively. Conclusion IDUS exhibited excellent sensitivity and specificity, while brush cytology revealed perfect specificity and positive predictive value on diagnosis of malignant biliary stricture. If IDUS combined with brush cytology, the sensitivity and negative predictive value could rise significantly, while the specificity and positive predictive value mildly went down, which suggested that IDUS remained a certain degree of over judgment and false positive rate. Key words: Endosonography; Cholangiopancreatography, endoscopic retrograde; Biliary stricture; Brush cytology

Role of Bronchial Brush Cytology in Diagnosis of Central Bronchial Carcinoma

Background : Despite the advances in imaging techniques and treatment modalities, the prognosis of lung cancer remains poor, with five-year survival of 14% at early stages and less than 5% in locally advanced stages. Before starting treatment a clear distinction between small cell and nonsmall cell carcinoma must be made, for that histopathology remains the mainstay of confirmation of diagnosis. To combat the disease successfully, it should be diagnosed at earliest possible stage. If bronchial brush cytology yield comparable diagnostic specificity, then it may easy for the patient and physician to manage this fatal disease.Purpose : The study was designed to evaluate the diagnostic yield of bronchial brush cytology in central (endoscopically visible) bronchial carcinomaSubjects and methods: This cross sectional study was done in the Department of Medicine, and Respiratory Medicine units of Sylhet MAG Osmani Medical College Hospital, Sylhet during the period from July 2012 to June 2014.Thirty seven patients with clinically suspected bronchial carcinoma were enrolled in this study fulfilling the inclusion and exclusion criteria. In all cases bronchial brush cytology and endo-bronchial biopsy were performed and results of both techniques were comparedReasults: The mean age of the patients with bronchial carcinoma was 59.65 (SD 12.95) years; 28 (75.7%) patients were 51 years or above with a ratio of male to female of 17.5:1. The sensitivity of bronchial brush cytology in the diagnosis of central bronchial carcinoma was 89.2% and that of endobronchial biopsy was 67.6%, no true negative cases were detected. Five (13.5%) patients developed respiratory distress and minor bleeding in 4 (10.8%) as a complications of bronchoscopy procedure.Conclusion: The diagnostic yield of bronchial brush cytology is non inferior to than that of endobronchial biopsy in the diagnosis of central bronchial carcinoma.J Bangladesh Coll Phys Surg 2017; 35(2): 68-74

Evaluation of Bile Ductal Brushing Cytology in Diagnosis of Extrahepatic Biliary Tract and Pancreatic Ductal Carcinoma

Evaluation of Bile Ductal Brushing Cytology in Diagnosis of Extrahepatic Biliary Tract and Pancreatic Ductal Carcinoma

Su1366 Biliary Brush Cytology for Diagnosis of Cholangiocarcinoma in Primary Sclerosing Cholangitis -a Systematic Review and Meta-Analysis

Su1366 Biliary Brush Cytology for Diagnosis of Cholangiocarcinoma in Primary Sclerosing Cholangitis -a Systematic Review and Meta-Analysis

Usefulness of imprint and brushing cytology in diagnosis of lung diseases with flexible bronchoscopy

Usefulness of imprint and brushing cytology in diagnosis of lung diseases with flexible bronchoscopy

Usefulness of imprint and brushing cytology in diagnosis of lung diseases with flexible bronchoscopy

BackgroundTo increase the diagnostic yield in pulmonary diseases, histopathology, imprint cytology and brushing cytology are assessed in combination during flexible bronchoscopy. However, the individual diagnostic discrimination of the three methods...

P74. Accuracy and patient acceptance of brush cytology for diagnosis of potentially malignant lesions and oral cancer

P74. Accuracy and patient acceptance of brush cytology for diagnosis of potentially malignant lesions and oral cancer

Diagnostic benefit of biliary brush cytology in cholangiocarcinoma in primary sclerosing cholangitis

Primary sclerosing cholangitis (PSC) is associated with a high risk of cholangiocarcinoma development. Efforts should be made to detect early neoplastic changes that can be radically treated by liver transplantation. In this prospective case series we obtained brush cytology specimens from bile duct strictures in 61 consecutive PSC patients. The cytological classifications were compared with histopathological findings in bile ducts from explanted livers or clinical outcome. Among patients with cytological low-grade (n=9; 15%) or high-grade dysplasia/adenocarcinoma (n=13; 21%), 8 (36%) proved to have cholangiocarcinoma and 7 (32%) to have high-grade dysplasia (i.e. cholangiocarcinoma in situ) in bile ducts from explanted livers. The sensitivity, specificity, positive- and negative predictive values, and accuracy of brush cytology in diagnosis of biliary malignancy were 100%, 84%, 68%, 100%, and 88% for the combination of low-grade and high-grade dysplasia/adenocarcinoma and 73%, 95%, 85%, 91%, and 90% for high-grade dysplasia/adenocarcinoma only. All patients with high-grade biliary epithelial dysplasia in explanted bile ducts were tumour free at follow-up. Brush cytology from bile duct strictures in PSC patients can detect cholangiocarcinoma in situ. Patients with cytological low-grade and high-grade dysplasia/adenocarcinoma are currently referred for liver transplantation in our hospital.