Actuality. The herb of Monarda fistulosa L. contains the essential oil and flavonoids, which are of the greatest interest in terms of pharmacological properties. The herb of Monarda fistulosa L. contains a number of flavonoids (rutin, hesperidin, diosmin, quercetin, luteolin, luteolin-7-O-glucoside, naringenin, catechin), however, the literature on the flavonoid composition of this plant is contradictory. In turn, this gives rise to a variety of approaches to standartization of the herb Monarda fistulosa L.
 The aim is to study the flavonoid composition of Monarda fistulosa L. by means of column chromatography and high performance liquid chromatography (HPLC).
 Materials and methods. The herb Monarda fistulosa L. was collected during the flowering period at the Botanical Garden of Samara University in 2018. Individual substances were isolated by means of column chromatography. Bruker DRX 500 instrument (126.76 MHz) was used to determine 13C NMR spectra, and Bruker AM 300 instrument (300 MHz) was used to determine 1H NMR spectra. Mass spectra were recorded by mass-spectrometer Kratos MS-30, UV spectra were recorded by means of the spectrophotometer Specord 40 (Analytik Jena). HPLC analysis was carried out with the use of chromatograph Milichrom-6 (NPO Nauchpribor).
 Results. For the first time column chromatography by means of 1H-NMR, 13C-NMR, UV spectroscopy, mass spectrometry allowed to isolate from Monarda fistulosa L. and characterize the following flavonoids: isorhoifolin (7-O-rutinoside of apigenin), linarin (7-O-rutinoside of acacetin), didymin (7-O-rutinoside of isosakuranetin), monardoside (5-O-rutinoside of apigenin), apigenin, acacetin. The composition of flavonoids was studied by means of HPLC method and isolated individual substances.
 Conclusion. The results can be used to develop a pharmacopoeial monograph for a new type of raw material Monarda fistulosa herb.