Bruguiera Cylindrica Research Articles

Characterization of heterogeneous groups of fungal endophytes isolated from mangroves in Negombo, Sri Lanka

Mangroves are well known for their adaptability to extreme conditions; thus, mangrove habitats help to manage coastlines and carbon cycling against anthropogenic stresses. Fungal endophyte is a type of microorganism that typically has a mutualistic relationship with its plant hosts. These endophytes can be found in several parts of the plant and play a positive role in the production of enzymes, nutrients and secondary metabolites, along with other processes such as toxin assembly and phyto-stimulation. The exploitation of these bioactive substances from endophytic fungi is beneficial in several fields such as pharmaceuticals and agriculture. The objective of the current investigation was to isolate and identify fungal endophytes present in ten mangrove species from Negombo, Sri Lanka: Acanthus ilicifolius, Acrostichum aureum, Avicennia marina, Bruguiera cylindrica, Bruguiera gymnorrhiza, Excoecaria agallocha, Lumnitzera racemosa, Rhizophora mucronata, Sonneratia alba and Sonneratia caseolaris. A standard potato dextrose agar culturing was used to obtain fungal colonies from leaves, roots and twigs, whose morphological characteristics were used for their identification. Out of the 42 fungal isolates extracted from the samples, nine endophytic genera such as Aspergillus, Candida, Chaetomium, Fusarium, Mucor, Penicillium, Phyllosticta, Rhizopus and Trichoderma were found. The highest number of isolates was obtained from Sonneratia alba. Each mangrove species held varying types of fungal colonies, with the genus Penicillium being isolated the highest number of times and the genus Aspergillus being highly diverse. The findings of this study have helped to confirm the presence of endophytic fungi in all the sample mangroves tested.

Evaluation of Antibacterial Activity and Characterization of Phytochemical Compounds from Selected Mangrove Plants

The aim of this study was to investigate the antibacterial activity of n-butanol, petroleum ether, ethyl acetate, ethanol, methanol, and chloroform extracts of the sponges and leaves of different mangrove species (Bruguiera cylindrica, Suaeda maritima, Ceriops decandra, Avicennia officinalis, Rhizophora apiculata, Suaeda monoica, Avicennia marina, and Rhizohora mucronata) against Proteus mirabilis ATCC 7002. Methods and Results: Agar well diffusion method was used to evaluate the antimicrobial activity of selected mangrove extracts. The active compounds from mangrove sponges and leaves with various solvents were examined under Fourier transform infrared (FTIR) spectroscopy. The ethyl acetate extracts, methanol extracts, chloroform extracts, n-butanol extracts, and petroleum ether extracts of selected mangrove leaves (B. cylindrical, S. maritime, A. officinalis, S. monoica, A. marina, R. mucronata) showed good antibacterial activity against P. mirabilis ATCC 7002. FTIR analysis of crude methanol extract of S. monoica showed the presence of amide and alkane groups in phytochemicals. For crude methanol extract of R. mucronata, the presence of phenol, nitro, amide, and alkane groups was revealed. Conclusion: the results obtained revealed industrially important mangrove extracts and the functional groups of plant compounds responsible for great antibacterial activity.

Variabilities of the carbon storage of mangroves in Gili Meno Lake, North Lombok District, Indonesia

Abstract. Hilyana S, Rahman FA. 2022. Variabilities of the carbon storage of mangroves in Gili Meno Lake, North Lombok District, Indonesia. Biodiversitas 23: 5862-5868. Mangrove is one of the coastal vegetation that can act as carbon mitigation (carbon sink and carbon storage). This study aims to determine the potential for carbon sinks and storage in the leaves and roots of each type of mangrove found in Gili Meno lake, North Lombok, Indonesia. The research includes the identification of species and sampling (leaves and roots) of mangroves in the research quadrant. The organic carbon content of mangrove leaves and roots was tested using the Wakley and Black method. The results showed that there were 5 (five) types of mangroves in Gili Meno lake, namely: Avicennia marina, Lumnitzera racemosa, Bruguiera cylindrica, Rhizophora apiculata, and Excoecaria agallocha. The highest leaf tissue carbon content value was R. apiculata at 45.85%C or equivalent to 3.19 g.C, while in roots, A. marina was 50.06%C, equivalent to 4.49 g.C. In addition, the potential carbon stock in the leaves of the entire mangrove ecosystem in an area of 3 ha is 762.81 tons.C±199.257 and at the roots is 659.76 tons.C±394.848, while the largest potential carbon stock in leaf organs is the type of mangrove R. apiculata, which is 318.91 tons.C.ha-1. and at the root is the type of mangrove A. marina, amounting to 448.54 tons.C.ha-1. The estimated carbon dioxide uptake by the Gili Meno mangrove leaves is in the range of 130.36 g.CO2-168.27 g.CO2 or with an average of 154.34 g.CO2±14.376, while the species with the highest carbon dioxide absorption capacity is R. apiculata (268.27 g.CO2) and the lowest in the species of L. racemosa (130.36 g.CO2).

STRUCTURE AND COMPOSITION OF FLORAL AT MANGROVE FOREST IN PITAS SABAH MALAYSIA

The aim of the study was to determined and record mangrove species composition anddiversity at Pitas mangrove forest, Sabah. Mangroves forest occurs in waterlogged, saltysoils of sheltered tropical and subtropical shores. It usually found along the coastlinesthroughout the world, usually between 25º N longitude and 25º S latitude. This study wasconducted in Pitas district (N 06?43’, E 117?4’) which is located at the northern tip ofSabah, Malaysia. Whereas, Pitas district has an approximately 38,564 ha of the mangrovearea. In this study, the mangrove location covered an area ranges from Malawali island(N 07?2.023', E 117?16.883'), Tobo island (N 07?01.070', E 117?19.008'), Layak-layakisland (N 06?56.587', E 117?14.323'), Mapan-mapan (N 06?51.608', E 117?14.861'), andJambongan island (N 06?45.016', E 117?25.816'). The random sampling method wasdone with simple plots sizes of 20m x 20m to determine the species’ biodiversity,composition and structure. As a result, 13 mangrove species have been identified in allstudy locations namely known as Sonneratia caseolaris, Ceriops tagal, Bruguieragymnorhiza, Rhizophora mucronata, Aegiceras corniculatum, Avicennia marina,Rhizophora apiculata, Sonneratia alba, Bruguiera cylindrica, Lumnitzera littorea,Pemphis acidula, Scyphiphora hydrophyllacea, and Scaevola taccada. The result showsthat the Shannon –Wiener biodiversity index (H’) at the island and disturbed mangroveareas is less diverse (H’ below than 1.5) compared to the undisturbed mainland areaswith (H’=1.95). Sonneratia caseolaris is the dominant mangrove species in the islandwith the highest value of Important Value Index (IVI) with 144.77% compared to allspecies in the study location. These areas show the importance of ecological aspectswithin the forest ecosystem. Therefore, the protection and conservation of this mangrovein this area is a necessity.Keywords: Mangrove, species composition, diversity, Important Value Index (IVI),island, mainland, disturbed, Pitas Sabah

Species Composition and Hydrolase Enzyme (EC.3) Activity of Fungi Isolated from Thasala Mangroves, Nakhon Si Thammarat Province, Southern Thailand

In the present study, we aimed to investigate diversity of manglicolous and endobiotic fungi associated with mangrove plants from Thasala mangroves, Nakhon Si Thammarat province, southern Thailand. Extracellular hydrolase enzymes (EC.3) including amylase, cellulase, lipase, protease, and pectinase from isolated fungi were evaluated. A total of 31 obligate marine fungal species was recorded from various Thasala mangroves sites. These included 24 ascomycetes (77.4 %), 1 mucoromycete (3.2 %), 3 basidiomycetes (9.7 %), 4 asexual morphs (12.9 %), and tentatively identified fungi (9.7 %). The average percentage occurrence of the fungi recorded from each site ranged from 0.4 to 16%. At all sites, the common filamentous marine fungi were Cumulospora sp., Halorosellinia oceanica, Kallichroma tethys, Leptosphaeria sp., Rimora mangrovei, Lulworthia sp., Phoma sp., Halenospora varia, Halocyphina villosa, Nia vibrissa, and Sclerococcum haliotrephum. Also, the most frequent marine fungi include Eutypella naqsii, Sammeyersia grandispora, Marinosphaera mangrovei, Nemania maritima, and Verruculina enalia. Seven mangrove plants were selected for endophytic fungal isolation including Acanthus ebracteatus, Acrostichum aureum, Avicennia alba, Bruguiera cylindrica, Rhizophora apiculata, R. mucronata, and Sonneratia alba. The isolation rate (IR) and colonization rate (CR) of fungal endobiota varied for all plant studied. The isolation rate ranged from 24 - 53.3 %, while colonization rate varied from 29.3 - 61.3 %. The fungal isolates were screened for the production of hydrolase enzymes (EC.3), of which 37.2 % exhibited enzyme activities. The 16 out of 28 obligate marine fungi (57.1 %) and 13 out of 50 fungal endophytes (26 %) had enzyme production efficiency toward specific substrates or an enzymatic index (EI) higher than 1.4. None of the fungal isolates depicted lipase activity. Additionally, the environmental stressors (temperature, pH, salinity) affecting enzyme production were evaluated in order to discover potential candidates for industrial application.
 HIGHLIGHTS
 
 Diversity of obligate marine fungal species was estimated from Thasala mangroves
 The obligate marine fungi and fungal endophytes had hydrolase enzyme (EC.3) production efficiency toward specific substrates or an enzymatic index (EI) higher than 1.4
 The temperature, pH, and salinity affecting enzyme production were evaluated in order to discover potential candidates for industrial application
 
 GRAPHICAL ABSTRACT

MOSQUITO LARVICIDAL POTENCY OF SELECTED HALOPHYTE SPECIES AND THEIR MODULATION ON ACETYLCHOLINESTERASE AND GLUTATHIONE S-TRANSFERASE AGAINST DENGUE VECTOR: Aedes aegypti

A mangrove is an ecosystem that represents a natural product reservoir of biotechnological interest. They have been advocated as environmentally benign alternatives to synthetic pesticides against all vector mosquitos. The purpose of this study was to determine the larvicidal activities of selected mangrove plant extracts against Aedes aegypti mosquito larvae. Methanol extracts of three selected halophytes viz. Lumnitzera racemosa, Bruguiera cylindrica, and Excoecaria agallocha were made, phytochemically investigated and evaluated against A. aegypti early 4th instar larvae. Acetylcholinesterase (AChE) activity and Glutathione S-transferase (GST) activity were evaluated, followed by DNA extraction and fragmentation tests. GC-MS technique was used to determine the presence of bioactive chemicals. Alkaloids, flavonoids, terpenoids, anthocyanin, coumarins, phenolic compounds, and quinones were found in all plants. The best larvicidal activity was recorded in the L. racemosa flower buds (LC50- 0.766 mg/ml) and E. agallocha stem extract (LC50- 0.986 mg/ml). L. racemosa flower buds and E. agallocha stem exhibited higher inhibition in Acetylcholinesterase (AChE) activity with the rate of 1.39 μmol ACT/min/mg protein and 1.65 μmol ACT/min/mg protein (p < 0.05) in treated larvae. In the case of L. racemosa flower buds, however, enhanced GST activity was detected. Plant extracts were found not to cause any fragmentation activity in the larval DNA. 5 major peaks of chemical compounds were analyzed in L. racemosa and E. agallocha. Because of the possible larvicidal action of selected halophyte extracts, they can be utilized as ecologically friendly mosquitocide for eradicating dengue vector larvae in their early stages.

Genetic diversity and population structure of Bruguiera cylindrica along coastal areas in Thailand

Bruguiera cylindrica (L.) Blume is an important true mangrove species and is the common mangrove species in Thailand. Studies related to the genetic diversity and population structure of B. cylindrica are limited. Here, the genetic diversity and population structure of 78 B. cylindrica accessions from the mangrove forests in Thailand were explored using 2611 single nucleotide polymorphism (SNP) markers. A low level of genetic diversity was inferred from the genetic diversity parameters (Ho = 0.289, He = 0.257, and I = 0.400). The results of STRUCTURE, a principal component analysis (PCA) and a maximum-likelihood (ML) tree suggested that the genetic structure of the studied B. cylindrica populations fell into two clusters corresponding to geographical regions. The analysis of molecular variance (AMOVA) pointed to higher levels of variation within populations than between populations, and the mean pairwise genetic differentiation (FST = 0.225) indicated high genetic population differentiation. This study revealed the genetic variation of B. cylindrica along the coast of Thailand for the first time and also provides valuable genetic information for this species that will be helpful for management and conservation of the species.

Mangrove species of Mantehage Island, Bunaken National Park, North Sulawesi, Indonesia

Abstract. Djamaluddin R, Djabar B. 2022. Mangrove species of Mantehage Island, Bunaken National Park, North Sulawesi, Indonesia. Biodiversitas 23: 2845-2852. A study on mangrove species in Mantehage Island, Bunaken National Park, North Sulawesi, Indonesia, is important due to the large extent of mangrove forest, geographical position, and geomorphological characteristics of the island. Many mangrove species are expected to occur on this island. This study was conducted to explore the biological diversity of mangrove species and to evaluate the biogeography and conservation status of certain species occurring on this island. Extensive surveys were conducted throughout the island on the areas covered with mangroves, with a total of 58 locations surveyed using spot check and quadrat-transect methods, and species identification was based on morphological characteristics. Results showed that in Mantehage Island there were 20 species of true mangrove belonging to 13 genera and 11 families, including Acanthus ilicifolius L., Acrostichum spesiosum Willd., Aegiceras corniculatum (L.) Blanco, Avicennia marina (Forsk.) Vierh., Bruguiera gymnorrhiza (L.) Lamk., Bruguiera parvilora Weight & Arnold ex Griffith, Ceriops tagal (Perr.) C.B. Rob, Excoecaria agallocha L., Heritiera littoralis Dryand, Nypa fruticans (Thunb.) Wurmb., Rhizophora apiculata Bl., Rhizophora mucronata Lamk., Rhizophora stylosa Griff., Scyphiphora hydrophyllacea Gaertn., Sonneratia alba J. Smith, Sonneratia ovata Blake., Xylocarpus granatum K?enig. Three rare species in the mangrove of Bunaken National Park, Camptostemon philippinense (Vidal) Becc., Bruguiera cylindrica (L.) Bl. and Lumnitzera racemosa Willd. were also recorded. The presence of C. philippinense could explain its distribution limit on the northern coast of North Sulawesi. Indication of putative hybrids in the genera of Rhizophora was found, but this needs further investigation. Two species of conservation concern, C. philippinense and S. ovata, are categorized as endangered and near-threatened species, respectively.

In situ conservation of mangroves species in Bagan Datuk, Perak

In situ conservation is one of the methods recommended for conserving forest genetic resources. It aims to maintain and recover viable population of species in its natural habitat, to reintroduce the rare and endangered species and to recreate the habitats for plant species. Though mangrove rehabilitation programmes are widely carried out in Malaysia, the most common species planted for this purpose have always been Rhizophora apiculata (Bakau minyak) and Rhizophora mucronata (Bakau kurap). In this study, mangroves species namely Bruguiera cylindrica (Berus-berus), Ceriops tagal (Tengar), Xylocarpus granantum (Nyireh bunga), Xylocarpus moluccensis (Nyireh batu) and Sonneratia ovata (Gedabu) were planted in Bagan Datuk Mangrove Germplasm, Perak. These species were selected due to their unavailability in the study area. The main objective of this study is to rehabilitate and to introduce new species in the Bagan Datuk Mangrove Germplasm area. The initial growth and survival of saplings planted were measured and data obtained were analyzed using SPSS program. After 12 months of planting, the survival rate was 80% and Sonneratia ovata recorded the highest mean height increment.

Metabolite profiling of Bruguiera cylindrica reveals presence of potential bioactive compounds

Bruguiera cylindrica parts are commonly used in Chinese and Indian traditional medicine to treat diarrhea, fever, and many ailments. The present study aims non targeted analysis of key secondary metabolites of B. cylindrica by gas chromatography mass spectrometry (GC-MS) and ultra-high performance liquid chromatography hybrid quadrupole-Exactive-Orbitrap high resolution mass spectrometry (UHPLC-Q-Exactive Orbitrap HRMS). GC-MS and UHPLC-Q-Exactive Orbitrap HRMS were utilized for metabolic profiling of ethyl acetate extract of B. cylindrica leaves. Key metabolites in the extract were identified and predicted based on chemical similarity using online databases such as ChemSpider and mzCloud. Thirty-six compounds belonging to different classes of secondary metabolites viz. flavonoids, fatty acids, fatty acid amides, carboxylic acids, and alkaloids were identified in the extract. Pentacyclic triterpenes like betulin, ursolic acid and a tropine, an alkaloid with potential pharmacological and therapeutic activities such as anticancer properties, neuromuscular blockers and antioxidants, were also identified. This study combined GC-MS and UHPLC-Q-Exactive Orbitrap HRMS with available online database for effective and rapid identification of bioactive metabolites in the ethyl acetate extract of mangrove without individual standard application. This is the first report on the HRMS based secondary metabolic profiling of B. cylindrica, with comprehensive map of its biologically important metabolites.

Assembly of a hybrid mangrove, Bruguiera hainesii, and its two ancestral contributors, Bruguiera cylindrica and Bruguiera gymnorhiza

Mangroves are plants that live in tropical and subtropical coastal regions of the world, they are adapted to high salt environments and cyclic tidal flooding. Mangroves play important ecological roles, including acting as breeding grounds for many fish species and to prevent coastal erosion. The genomes of three mangrove species, Bruguiera gymnorhiza, Bruguiera cylindrica, and a hybrid of the two, Bruguiera hainesii were sequenced, assembled and annotated. The two progenitor species, B. gymnorhiza and B. cylindrica, were found to be highly similar to each other and sufficiently similar to B. parviflora to allow it to be used for reference based scaffolding to generate chromosome level scaffolds. The two subgenomes of B. hainesii were independently assembled and scaffolded. Analysis of B. hainesii confirms that it is a hybrid and the hybridisation event was estimated at 2.4 to 3.5 million years ago using a Bayesian Relaxed Molecular Clock approach.

ANALISIS FAKTOR EKOLOGIS VEGETASI MANGROVE DI NEGERI ETI TELUK PIRU KABUPATEN SBB

This study aims to determine the types of mangrove vegetation and the factors where mangroves grow in the land of Eti, Teluk Piru, West Seram Regency. The methods used in the measurement and observation in this study were descriptive and field surveys. Based on the results of mangrove research in the country of Eti, Teluk Piru, West Seram Regency, the types of vegetation that make up the mangrove ecosystem at the research site are: Sonneratia alba, Rhizophora apiculata, Rhizophora stylosa, Bruguiera gymnorrhyza, Bruguiera cylindrica, Avicennia officinalis, Xylocarpus granatum, Nypa fruticaria. agallocha and Scyphiphora hydrophyllacea and the measurement results of environmental parameters that affect mangroves, namely. Temperature: the lowest temperature range is 27oC to the highest temperature is 33oC. Salinity: the lowest salinity level is 21 ppt to the highest salinity is 33 ppt. The degree of acidity (pH) of water: the range of the lowest water pH is 5.5 to the highest temperature 8. The degree of acidity (Ph) of the soil: the range of the lowest water pH is 5 to the highest temperature 8. Subsrak: the condition of the soil at the study site has a type of substrate mud up to sandy mud. DO: the average DO front is 2.9 mg/1 and the average DO back is 2.8 mg/1 in the results obtained the average DO value is 5.3 mg/l. mud thickness: the average thickness of the front mud is 43 cm, the average thickness of the middle mud is 31 cm and the average thickness of the rear mud is 29 cm.

Aerial surveys reveal biotic drivers of mangrove expansion along a Thai salt flat ecotone

Rapid sea level rise (SLR) could overwhelm the adaptive capacity of many mangrove forests. The conservation of functional mangrove ecosystems will rely on their expansion into upper intertidal areas, including salt marshes and salt flats, in advance of SLR. The development of new mangrove habitats could be accelerated by amplifying positive biotic interactions among herbaceous plants, macrofauna, and mangrove trees, such as trapping of hydrochorous propagules or site amelioration. It was investigated how biotic and abiotic factors influenced the expansion of mixed‐species mangrove stands along a mangrove‐salt flat ecotone. Multi‐temporal unmanned aerial vehicle (UAV) imagery (< 1 cm/pixel) was used to monitor seedlings of Avicennia alba, Bruguiera cylindrica, and Lumnitzera racemosa in three sample plots (0.7–0.9 ha) over 2 years. The aerial images were used to map mangrove crowns, sedges (Fimbristylis tetragona), substrate elevation, and microtopography. The spatial distribution of seedlings was modeled using point pattern processes; mixed‐effects models were used to analyze how environmental factors influenced seedling survival and growth. The spatio‐temporal patterns of mangrove regeneration revealed that sedges and macrofauna facilitated the regeneration of mangroves into higher‐elevated areas of low tree density. Sedges not only trapped seeds but also increased the likelihood of survival among seedlings of all species. Large‐sized propagules established in undulating microtopography created through the bioturbation of macrofauna. Herbaceous vegetation expanded the range of macrofauna in the absence of trees. Thus, the migration of mangroves into hypersaline retreat areas may be accelerated by enhancing the growth of herbaceous vegetation and promoting burrowing macrofauna.

Mangrove seedling abundance in mangrove ecosystem outgrowth in Ajkwa Island, Mimika Regency, Papua

Mangrove is one of the essential ecosystems for coastal areas. Mangrove is an individual or community which survives in tidal areas. Ajkwa Island is an island created by sedimentation activities from Ajkwa river. Ajkwa Island has a tidal supply well and it makes this island be an essential area for mangrove growth. This research was conducted in Ajkwa Island, Mimika Regency, Papua Province. The research is in permanent monitoring plots in 2011, 2014, and 2018. Based on the research, 2 categories were found in the monitoring plot, there are seedling and herbs. Species for seedling category are Avicennia marina, Bruguiera gymnorrhiza, Bruguiera cylindrica, Diospyros sp., Nypa fruticans, Sonneratia alba, and Xylocarpus sp. Species for herbs category are Allamanda cathartica and Hoya sp. Mangrove ecosystem type in Ajkwa Island is over-wash mangrove. The result, Sonneratia alba and Bruguiera gymnorrhiza had density and important value index higher than the other species in every monitoring year. Mangrove spreading in Ajkwa Island increases in each monitoring year. Mangrove seedling spread significantly from 2014 to 2018. On the monitoring plots, mangrove density in 2014 is 41.700 ind/ha and for 2018 is 74.000 ind/ha. Beside vegetation analysis, this research does spatial analysis to support the result from field monitoring data. Spatial analysis also proved the spreading of the seedling increased significantly from 2014 to 2018.

Engaging multi-stakeholders’ strategy toward strengthened mangrove rehabilitation program in Tangerang Regency

The Tangerang’s coastal area faces (1) decreasing environmental quality, and (2) poverty. The government developed Gerbang Mapan Program, an integrated coastal management (ICM) program designed to accelerate the recovery of coastal ecosystems and community development. To encourage the acceleration of the program, the government invites various parties (multistakeholders) to be involved in the program. This study aims to explore the processes of involving multistakeholders in the success of the mangrove rehabilitation, which has now succeeded transforms from damage to be good and have even become centers of community socio-economic activities. The method is an explorative method with a coevolutive dynamic historical process approach from 2014 to 2020. The involvement of various parties i.e local government agencies/institutions in Tangerang Regency, the private sector, TNI/Polri, schools, universities, and coastal community groups in mangrove rehabilitation programs and awareness campaigns and public education have encouraged the acceleration of mangrove rehabilitation, where by 2020, rehabilitation has been carried out with planted 720,000 mangrove trees, with six species (Rhizophora sp, Avicennia sp, Bruguiera cylindrica, Bruguiera gymnoriza, Ceriop tagal and Sonneratia alba), where the survival rate reached 69.22%, with the most adaptive type Avicennia sp planted in Tangerang Regency with a survival rate of 90.71%.

Mangrove ecology and species distribution along the Gorai Creek of Mumbai coast, Maharashtra, India

The extensive mangrove forest of Gorai Creek, Mumbai coast, has recently seen substantial deterioration. The results of a study on mangrove diversity conducted in Gorai Creek on the Mumbai coast from June 2017 to May 2018 are presented here. During the course of the study, twelve species of mangroves from five families and eight genera were identified in each of the three study locations along the creek. Avicennia marina accounted for 13.44% of the Gorai creek marshes, confirming its predominance. Sonneratia alba, Avicennia officinalis, Rhizophora apiculata, Bruguiera cylindrica, Kandelia candel, and Acanthus ilicifolius are among the mangrove species found in the estuarine embayment, with other species strewn around. A diverse range of species, including endangered migratory birds and herpetofauna, can be found in these mangrove environments. Mangroves have been observed to have narrowed in density with time, and it is critical to begin conservation efforts as speedily as humanly possible.

Recent advances on therapeutic potentials of gold and silver nanobiomaterials for human viral diseases.

Viral diseases are prominent among the widely spread infections threatening human well-being. Real-life clinical successes of the few available therapeutics are challenged by pathogenic resistance and suboptimal delivery to target sites. Nanotechnology has aided the design of functionalised and non-functionalised Au and Ag nanobiomaterials through physical, chemical and biological (green synthesis) methods with improved antiviral efficacy and delivery. In this review, innovative designs as well as interesting antiviral activities of the nanotechnology-inclined biomaterials of Au and Ag, reported in the last 5 years were critically overviewed against several viral diseases affecting man. These include influenza, respiratory syncytial, adenovirus, severe acute respiratory syndromes (SARS), rotavirus, norovirus, measles, chikungunya, HIV, herpes simplex virus, dengue, polio, enterovirus and rift valley fever virus. Notably identified among the nanotechnologically designed promising antiviral agents include AuNP-M2e peptide vaccine, AgNP of cinnamon bark extract and AgNP of oseltamivir for influenza, PVP coated AgNP for RSV, PVP-AgNPs for SARS-CoV-2, AuNRs of a peptide pregnancy-inducedhypertension and AuNP nanocarriers of antigen for MERS-CoV and SARS-CoV respectively. Others are AgNPs of collagen and Bacillus subtilis for rotavirus, AgNPs labelled Ag30–SiO2 for murine norovirus in water, AuNPs of Allium sativum and AgNPs of ribavirin for measles, AgNPs of Citrus limetta and Andrographis Paniculata for Chikungunya, AuNPs of efavirenz and stavudine, and AgNPs-curcumin for HIV, NPAuG3-S8 for HSV, AgNPs of Moringa oleifera and Bruguiera cylindrica for dengue while AgNPs of polyethyleneimine and siRNA analogues displayed potency against enterovirus. The highlighted candidates are recommended for further translational studies towards antiviral therapeutic designs.

Short Communication: Structure and composition of mangrove vegetation in Lembar bay area, West Lombok District, Indonesia

Abstract. Sukuryadi, Harahab N, Primyastanto M, Hadi AP. 2021. Short Communication: Structure and composition of mangrove vegetation in Lembar bay area, West Lombok District, Indonesia. Biodiversitas 22: 5585-5592. One of the potential natural resources of coastal areas in West Lombok District, West Nusa Tenggara Province, Indonesia is the 501.9-ha mangrove ecosystem. The purpose of this study was to analyze the structure and composition of mangroves in the Lembar bay area. The study used field observation methods based on predetermined plots. The results showed that the composition of mangroves in the Lembar Bay area consisted of Avicennia alba Bl, Avicennia marina (Forsk) Vierh, Bruguiera cylindrica (L) Bl, Ceriop decandra (Griff) Ding Hou, Ceriop tagal (Perr) C.B. Rob, Lumnitzera littorea (Jack) Voight, Lumnitzera racemosa Willd. Var, Phemphis acidula, Rhizhopora stylosa Griff, Rhizophora apiculata Bl, Rhizophora mucronata Lmk, and Sonneratia alba J.E. Smith. The mangrove vegetation at tree level was composed of 12 species dominated by Rhizhopora stylosa Griff and Rhizophora mucronata Lmk, with Importance Value Index (IVI) of 56.05 and 48.89, respectively, at sapling level 9 species dominated by Rhizhopora stylosa Griff, Bruguiera cylindrica, and Rhizophora mucronata, with IVI of 47.46, 33.33, and 31.07 respectively, and at the seedling level 7 species dominated by Rhizhopora stylosa Griff and Rhizophora mucronata Lmk, with IVI of 47.71 and 37.76, respectively.

Bioprospecting of Selected Mangrove Fruits Based-Nutritional, Antioxidant, and Element Properties to Support Functional Food Materials for Pulau Sembilan Coastal Communities, Indonesia

Mangrove plants are popularized for their edible and vital source of food. Mangroves in Pulau Sembilan, Langkat, North Sumatra, Indonesia were supported by the high plant diversity. These major tree species were provided in mangrove fruits annually and assist in finding a new variety of functional food materials. Bioprospecting was denoted the exploration of bioresources material to useful derived mangrove products. The present study aims to assess bioprospecting based-nutritional parameters, antioxidant content, and elemental analysis (micronutrient and macronutrient) in fruits of eight mangrove plants: <em>Avicennia officinalis</em>, <em>Bruguiera cylindrica</em>, <em>Rhizophora apiculata, Ceriops tagal, R. stylosa, R. mucronata,</em> <em>Xylocarpus granatum and Sonneratia alba </em>in Pulau Sembilan, North Sumatra, Indonesia. Out of seven nutritional parameters, <em>A. officinalis</em> recorded the highest in three parameters (protein, total sugar, and non-reducting sugar), <em>X. granatum</em> recorded highest in two parameters (fat and moisture content), among all studied species followed by<em> R. mucronata, </em>and <em>R. stylosa </em>in one parameter (ash and reducing sugar), respectively. Among the antioxidant content, <em>R. mucronata</em> exhibited the highest ascorbic acid content and phenolic acid. Beta carotenoid was maximum in <em>C. tagal</em>. The highest macro element varied among the mangrove fruits: sodium was recorded in <em>R. apiculata</em>, potassium content was noted in <em>X. granatum, </em>and calcium was in <em>S. alba. </em>Likewise, the highest microelement was spread among the mangrove fruits: <em>X. granatum </em>(iron), <em>R. mucronata </em>(manganese), <em>A. officinalis </em>(cupper), and <em>S. alba </em>(zinc). Thus, this study's findings showed the mangrove fruits have reported prospective values as antioxidants, bio-nutrition, and renewable food sources potential for the adjacent mangrove.

Antibacterial effects of mangrove ethanolic leaf extract against zoonotic fish pathogen Salmonella arizonae

The emergence of multiple drug-resistant pathogens, affecting aquaculture and public health, has put the spotlight on alternative medicine research. This study was conducted to evaluate the In vitro and In vivo antibacterial activity of mangrove ethanolic leaf extract (MLEE) against Salmonella arizonae isolated from Carassius auratus. In vitro, antimicrobial activity of 10 mangrove species and 13 commercial antibiotics were determined using the agar diffusion method. MLEE with the highest antimicrobial activity were subjected to qualitative phytochemical tests and bioassay experiments. In vivo antibacterial activity of MLEE was assessed using C. auratus intraperitoneally injected with S. arizonae. Results showed that Sonneratia alba has the highest antimicrobial activity against S. arizonae followed by Avicennia marina, A. officinalis, Sonneratia ovata, Rhizophora mucronata, Excoecaria agallocha, and Bruguiera cylindrica. However, bacterial isolate was resistant to A. rhumpiana, Scyphiphora hydrophyllacea, and Laguncularia racemosa. Interestingly, S. alba has comparable antimicrobial activity with amoxicillin, trimethoprim, novobiocin, and cefixime. The activity of S. alba could be attributed to the presence of flavonoids, saponin, sterols, tannin, and terpenoids. Moreover, S. alba has reduced and delayed the onset of goldfish mortality infected with S. arizonae. Based on these findings, the S. alba MLEE, is a potential antimicrobial resource against S. arizonae.