Body Odor Research Articles

Associations between olfactory reference disorder and social phobia - results of an internet-based study.

Despite the similar clinical features of Olfactory Reference Disorder (ORD) and Social Phobia (SP), or studies showing elevated comorbidity of the two disorders, and the conceptualization of ORD as a form of SP in the East Asian culture, to our knowledge, the relationship between ORD and SP has not been investigated. This study examined the association of ORD according to the 11th revision of the International Classification of Diseases (ICD-11) and SP in 225 German university / college students who completed self-ratings with regard to socio-demographic data and symptoms of SP and ORD within an anonymous internet-based survey. Symptoms of SP were assessed with the Social Phobia Inventory (SPIN). Symptoms of ORD according to the ICD-11 were assessed with the Olfactory Reference Disorder Questionnaire (ORDQ), developed for this study. In our sample, 86.6% of the participants who met the self-rated features for ORD also met the self-rated criteria for current SP. ORD severity scores were significantly related to SP. Participants with and without self-reported ORD differed significantly in their SP total scores. SP severity was also significantly correlated with poorer insight of ORD-related beliefs, greater ORD-related avoidance of intimate relationships and higher levels of shame and fear of rejection due to body odor. These preliminary findings indicate that ORD could be closely related to SP and highlight the need for future research on the relationship of ORD and SP in order to gain a better understanding of the development, maintenance, treatment and classification of ORD.

Switching from immediate- to extended-release cysteamine in patients with nephropathic cystinosis: from clinical trials to clinical practice.

The purpose of this study is to evaluate the effectiveness and safety of switching from immediate-release (IR) to extended-release (ER) cysteamine in patients with nephropathic cystinosis (NC) in Spain. We conducted an observational, retrospective, multicentre study in NC patients who received IR cysteamine for at least 12months, switched to ER cysteamine, and received it for at least 6months before inclusion. Data were collected from nine patients (four children, five adults) 36months before and after the switch. Despite the highly selected population, an improvement in growth, particularly in children and a significant reduction in hospitalization days was observed. A decrease in halitosis, body odour and gastrointestinal effects was reported in most of the patients who suffered before the switch, and the use of proton pump inhibitors (PPIs) decreased in some patients. The estimated glomerular filtration rate (eGFR) remained stable in patients with preserved kidney function. No significant changes in white blood cell (WBC) cystine levels were observed after the switch. There was no significant difference in the cysteamine dose received. However, some patients were receiving <50% of the recommended dose of cysteamine before and after the switch and showed elevated levels of WBC cystine. Switching from IR to ER cysteamine in clinical practice reduces hospital stays, improves nutritional status and growth in paediatric patients and could help to enhance treatment tolerability by reducing side effects. Furthermore, the dosing of ER cysteamine could promote therapeutic compliance and positively affect the quality of life of the NC population.

Body odours as putative chemosignals in the father-child relationship: New insights on paternal olfactory kin recognition and preference from infancy to adolescence

Children's body odours are effective chemical cues in the parent-child relationship. Mothers can recognize the odour of their child and prefer this odour over that of unfamiliar children. This effect is mediated by genetic similarity and developmental stage and is therefore suited to promote parental care at pre-pubertal stage, while facilitating incest avoidance at (post-)pubertal stage. The present study tested whether similar mechanisms apply to fathers. Therefore n = 56 fathers evaluated body odour samples of their own and of unfamiliar children in varying genetic and developmental stages. Genetic status was determined by human leucocyte antigen (HLA) profiling, developmental status by standardized assessment of pubertal status and steroid hormone concentration (estradiol, testosterone). Similar to mothers, fathers identified their own child's body odour above chance and preferred that odour. The paternal preference did not relate to HLA similarity but decreased with increasing age of the child. The decline was associated with higher pubertal stages in daughters only, which supports the hypothesis of odour-mediated incest prevention in opposite-sex parent-child dyads.

Formulasi dan Stabilitas Mutu Fisik Sediaan Lulur Serbuk dari Ampas Kopi Arabika (Coffea arabica) Gayo, Ubi Jalar Ungu (Ipomea batatas L) dan Rimpang Jahe (Zingiber officinale)

Scrub is a traditional cosmetic preparation that has been prescribed for generations and is used to remove dead skin cells, dirt, and open pores so that air exchange is free and the skin becomes brighter and whiter. Previous research reported that Gayo Arabica coffee grounds discarded as waste contain antibacterial and antioxidant compounds. Purple sweet potatoes are reported to contain natural antioxidants because they contain anthocyanins and beta-carotene, apart from that, ginger rhizomes are reported to be able to inhibit the growth of bacteria that cause body odor on human skin. Therefore, these three ingredients have the potential to be used as ingredients for making natural powder scrubs. This research aimed to determine the effect of the proportion of Gayo Arabica coffee grounds, purple sweet potato, and ginger rhizome on the finished product of powdered scrub preparations as seen from the stability of their physical quality. The research method used was experimental in the laboratory with a Completely Randomized Design (CRD). The powder scrub formulation is made in 3 formulas (F1, F2, and F3) by varying the ratio of the amount of Gayo Arabica coffee grounds, purple sweet potato, and ginger rhizome respectively, namely F1 (1:3:1), F2 (2:2: 1) and F3 (3:1:1). The weight of each formula is 100 g. The physical quality stability test of the powder scrub preparation was carried out on the 1st, 7th, and 14th days including organoleptics, water content, homogeneity, pH, drying time, and adhesion. The test results showed that during 2 weeks of storage, the body scrub preparations that were made had met the requirements for good physical quality. In the organoleptic test, the color, shape, and odor of the preparation did not change, the preparation was homogeneous, the pH was stable at 6.2, and the water content was stable at below 10% where the F3 formula showed the lowest water content. The results of the drying time test meet the requirements, namely a minimum of 15 minutes and the adhesion test meets the requirements, namely greater than 1 second.

Development of Soup Mix Powder Using Avarampoo (Senna Auriculata)

Avaram (Senna auriculata (L.) Roxb. syn. Cassia auriculata L.; Family: Fabaceae) is a traditional medicinal plant used in India’s Ayurveda and Siddha traditions of medicine to cure a variety of diseases. There have been reports of therapeutic uses for almost every part of the plant, such as the flowers, leaves, seeds, bark, and roots. Traditionally, it has been used to treat diabetes, asthma, rheumatism, dysentery, skin illness, and metabolic problems. The herb avaram is used to manufacture powdered soup mix. Tastes fantastic and an excellent substitute for energy drinks like soup is the antioxidant-rich mix. By functioning as a natural blood purifier, antioxidants shield the body and skin. Avaram poo soup is made with a mixture of dried and natural spices. It can also help with Blood sugar, Skin tone, Body odor, Menstrual cycle, Hair growth, Premature greying.

Sorption Kinetic Parameters from Nanomechanical Sensing for Discrimination of 2-Nonenal from Saturated Aldehydes.

Nanomechanical sensors have gained significant attention as promising platforms for artificial olfaction. Since sorption kinetic parameters that can be estimated from the sensing signals of nanomechanical sensors reflect the chemical and physicochemical interactions between the odorant and receptor material, the parameters can be utilized for the direct discrimination of each odorant. In this study, we demonstrated the discrimination of 20 vapors, including hydrocarbons, alcohols, organic acids, ketones, and aldehydes, which are reported as human body odor components, using the parameters extracted in the analytical solution of nanomechanical sensors based on sorption kinetics with viscoelastic behaviors. By using one of the specific nanomechanical sensors─membrane-type surface stress sensor─as a sensing unit, we successfully discriminated trans-2-nonenal known as an aging marker from other saturated aldehydes along with quantifying their concentrations.

Antibacterial and deodorizing effects of cold atmospheric plasma-applied electronic deodorant

Axillary odor is a malodor produced by bacterial metabolism near the apocrine glands, which often causes discomfort in an individual's daily life and social interactions. A deodorant is a personal care product designed to alleviate or mask body odor. Currently, most deodorants contain antimicrobial chemicals and fragrances for odor management; however, direct application to the underarm skin can result in irritation or sensitivity. Therefore, there is a growing interest in technologies that enable disinfection and odor control without the antiperspirants or perfumes. The cold atmospheric plasma temporally generates reactive radicals that can eliminate bacteria and surrounding odors. In this study, cultured Staphylococcus hominis and Corynebacterium xerosis, the causative bacteria of axillary bromhidrosis, were killed after 90% plasma exposure for 3 min. Moreover, the electronic nose system indicated a significant reduction of approximately 51% in 3-hydroxy-3-methylhexanoic acid and approximately 34% in 3-methyl-3-sulfanylhexan-1-ol, the primary components of axillary odor, following a 5-min plasma exposure. These results support the dual function of our deodorant in eliminating bacteria and axillary odors without the chemical agents. Therefore, cold atmospheric plasma-applied deodorant devices have great potential for the treatment and management of axillary odors as a non-contact approach without chemical use in daily life.

Formulation of Body Odor Bacteria Inhibiting Deodorant Spray from Ethyl Acetate Extract of Klika Kesambi

Acne is one of the skin damages caused by acne-causing bacteria. Flavonoids, saponins, and tannins in taro tubers can potentially have activity against acne-causing bacteria. This study aims to determine the formulation of extract transparent solid soap preparation that meets the characteristics and assess the activity of soap preparation as anti-acne against acne-causing bacteria. Taro tubers were extracted by maceration method. The extract was formulated as transparent solid soap preparations with variations in extract concentration, namely F0 0%, F1 2%, F2 4%, and F3 8%. The soap was evaluated for characteristics including organoleptic test, pH, moisture content, free fatty acids and alkali, mineral oil test, and foam stability test. Antibacterial activity testing of transparent soap extracts was carried out against acne-causing bacteria. The extraction results obtained an extract yield of 10.86%, the three extract formulas have transparent solid soap characteristics that meet the requirements. Soap preparation formula 1 has an inhibition zone of 21.66 ± 0.31 mm against P. acnes bacteria, 19.11±0.53 mm against S. epidermidis, 21.74±0.34 mm against S. aureus. Soap preparation formula 2 has an inhibition zone of 18.53±0.26 mm against P. acnes bacteria, 18.71±0.49 mm against S. epidermidis, 20.16±0.34 mm against S.aureus and soap preparation formula 3 has an inhibition zone of 19.03±0.68 mm against P. acnes bacteria, 19.93±0.86 mm against S. epidermidis, 22.75±0.59 mm against S.aureus. In conclusion, formula 2 with 2% extract concentration has the best soap characteristics and has the most optimal inhibition zone.

Antimicrobial Properties Related to Anti-Acne and Deodorant Efficacy of Hedychium coronarium J. Koenig Extracts from Pulsed Electric Field Extraction.

This study investigated the potential of pulsed electric field (PEF) extraction in enhancing the antimicrobial properties related to anti-acne and deodorant properties of Hedychium coronarium extract. The dried leaf and rhizome of H. coronarium were extracted using 95% v/v ethanol through both conventional solvent extraction and PEF extraction techniques (10, 14, and 20 kV/cm). The chemical composition of the extracts was analyzed. The antimicrobial activities, specifically in relation to acne treatment against Cutibacterium acnes and deodorant properties against Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa, and Escherichia coli, were determined. The irritation profile of was evaluated using the hen's egg chorioallantoic membrane test. The results showed that PEF extraction increased the extract yield, particularly at an electric field strength of 20 kV/cm. Furthermore, PEF extraction significantly enhanced the ellagic acid content, particularly in the leaf extract. Furthermore, the leaf extract demonstrated stronger inhibitory effects against microorganisms associated with body odor and acne compared to the rhizome extract. Notably, all extracts exhibited no signs of irritation, indicating their safety. Overall, the findings suggest that PEF extraction from H. coronarium enhances yield, bioactive compound content, and antimicrobial effects. This indicates the potential of the extract for acne treatment and deodorant use.

Music as aposematic signal: predator defense strategies in early human evolution.

The article draws attention to a neglected key element of human evolutionary history-the defense strategies of hominins and early humans against predators. Possible reasons for this neglect are discussed, and the historical development of this field is outlined. Many human morphological and behavioral characteristics-musicality, sense of rhythm, use of dissonances, entrainment, bipedalism, long head hair, long legs, strong body odor, armpit hair, traditions of body painting and cannibalism-are explained as predator avoidance tactics of an aposematic (warning display) defense strategy. The article argues that the origins of human musical faculties should be studied in the wider context of an early, multimodal human defense strategy from predators.

Influence of the human body odor compound HMHA on face perception.

Body odors convey information about the individuals, but the mechanisms are not fully understood yet. As far as human reproduction is concerned, molecules that are produced in sexually dimorphic amounts could be possible chemosignals. 3-hydroxy-3-methylhexanoic acid (HMHA) is one of them-more typical of men. Here, we investigated the possibility that the perception of gender and attractiveness in human faces could be implicitly influenced by this compound. Clearly feminine, ambiguous and clearly masculine faces were primed with an odor of HMHA, a control odor or air. Based on 100-ms face presentation, 40 raters had to identify the face's gender as quickly as possible and provide attractiveness evaluations. 3-hydroxy-3-methylhexanoic acid tended to be perceived as less pleasant and induced lower sniff duration in women compared with men. As to the effects of HMHA on face perception (vs. control conditions), we found that gender identification and the associated response time were unaffected by HMHA. Attractiveness of the faces, however, increased in presence of HMHA, but not in a sex-specific manner and only for unattractive faces with ambiguous gender. In sum, this study found no evidence in favor of a possible role of this sexually dimorphic compound in intrasexual competition nor in intersexual attraction.

An electronic nose can identify humans by the smell of their ear.

Terrestrial mammals identify conspecifics by body odor. Dogs can also identify humans by body odor, and in some instances, humans can identify other humans by body odor as well. Despite the potential for a powerful biometric tool, smell has not been systematically used for this purpose. A question arising in the application of smell to biometrics is which bodily odor source should we measure. Breath is an obvious candidate, but the associated humidity can challenge many sensing devices. The armpit is also a candidate source, but it is often doused in cosmetics. Here, we test the hypothesis that the ear may provide an effective source for odor-based biometrics. The inside of the ear has relatively constant humidity, cosmetics are not typically applied inside the ear, and critically, ears contain cerumen, a potent source of volatiles. We used an electronic nose to identify 12 individuals within and across days, using samples from the armpit, lower back, and ear. In an identification setting where chance was 8.33% (1 of 12), we found that we could identify a person by the smell of their ear within a day at up to ~87% accuracy (~10 of 12, binomial P < 10-5), and across days at up to ~22% accuracy (~3 of 12, binomial P < 0.012). We conclude that humans can indeed be identified from the smell of their ear, but the results did not imply a consistent advantage over other bodily odor sources.

Using surface-enhanced Raman scattering for simultaneous multiplex detection and quantification of thiols associated to axillary malodour.

Axillary malodour is caused by the microbial conversion of human-derived precursors to volatile organic compounds. Thiols strongly contribute to this odour but are hard to detect as they are present at low concentrations. Additionally, thiols are highly volatile and small making sampling and quantification difficult, including by gas chromatography-mass spectrometry. In this study, surface-enhanced Raman scattering (SERS), combined with chemometrics, was utilised to simultaneously quantify four malodourous thiols associated with axillary odour, both in individual and multiplex solutions. Univariate and multivariate methods of partial least squares regression (PLS-R) were used to calculate the limit of detection (LoD) and results compared. Both methods yielded comparable LoD values, with LoDs using PLS-R ranging from 0.0227 ppm to 0.0153 ppm for the thiols studied. These thiols were then examined and quantified simultaneously in 120 mixtures using PLS-R. The resultant models showed high linearity (Q2 values between 0.9712 and 0.9827 for both PLS-1 and PLS-2) and low values of root mean squared error of predictions (0.0359 ppm and 0.0459 ppm for PLS-1 and PLS-2, respectively). To test this approach further, these models were challenged with 15 new blind test samples, collected independently from the initial samples. This test demonstrated that SERS combined with PLS-R could be used to predict the unknown concentrations of these thiols in a mixture. These results display the ability of SERS for the simultaneous multiplex detection and quantification of analytes and its potential for future development for detecting gaseous thiols produced from skin and other body sites.

Italian validation of the body odor disgust scale.

Disgust sensitivity to body odors plays a role in a set of psychological mechanisms supposedly evolved to avoid pathogens. To assess individual differences in body odor disgust, we previously developed the body odor disgust scale (BODS) and validated it in English. The BODS presents six scenarios where disgust could be evoked by smells coming from an internal source and an external source. The present study aimed to validate the BODS in the Italian population and to find further evidence for its structural, construct, and criterion validity. We used two large samples (N = 1,050, F = 527; and N = 402, F = 203, respectively) that were representative of the Italian population for sex and age. Across these two studies, we confirmed the hypothesized bifactor structure, with all the items loading onto a general body odor disgust sensitivity factor, and on two specific factors related to the internal structure. In terms of construct validity, we found that the BODS converged with pathogen disgust sensitivity of the three-domain disgust scale (TDDS) but was distinct from a general propensity to experience negative emotions. The BODS showed criterion validity in predicting the behavioral intentions toward COVID-19 avoidance behavior, although it did not seem to be incrementally valid when compared to the TDDS pathogen subscale. We also established scalar measurement invariance of the BODS regarding gender and found that women display higher levels of BODS. Results from the Italian version of the BODS indicate its structural, construct, nomological and criterion validity. Furthermore, our result on sex differences in disgust sensitivity are consistent with previous literature, and we discuss them in the broader context of cross-cultural and primate findings that points toward a possible evolutionary explanation of this difference.

Vasopressin differentially modulates the excitability of rat olfactory bulb neuron subtypes.

Vasopressin (VP) plays a crucial role in social memory even at the level of the olfactory bulb (OB), where OB VP cells are activated during social interactions. However, it remains unclear how VP modulates olfactory processing to enable enhanced discrimination of very similar odors, e.g., rat body odors. Thus far, it has been shown that VP reduces firing rates in mitral cells (MCs) during odor presentation in vivo and decreases the amplitudes of olfactory nerve-evoked excitatory postsynaptic potentials (ON-evoked EPSPs) in external tufted cells in vitro. We performed whole-cell patch-clamp recordings and population Ca2+ imaging on acute rat OB slices. We recorded ON-evoked EPSPs as well as spontaneous inhibitory postsynaptic currents (IPSCs) from two types of projection neurons: middle tufted cells (mTCs) and MCs. VP bath application reduced the amplitudes of ON-evoked EPSPs and the frequencies of spontaneous IPSCs in mTCs but did not change those in MCs. Therefore, we analyzed ON-evoked EPSPs in inhibitory interneurons, i.e., periglomerular cells (PGCs) and granule cells (GCs), to search for the origin of increased inhibition in mTCs. However, VP did not increase the amplitudes of evoked EPSPs in either type of interneurons. We next performed two-photon population Ca2+ imaging in the glomerular layer and the superficial GC layer of responses to stronger ON stimulation than during patch-clamp experiments that should evoke action potentials in the measured cells. We observed that VP application increased ON-evoked Ca2+ influx in juxtaglomerular cells and GC somata. Thus, our findings indicate inhibition by VP on projection neurons via strong ON input-mediated inhibitory interneuron activity. This neural modulation could improve representation of odors, hence, better discriminability of similar odors, e.g., conspecific body odors.

Profil Kualitas Organoleptik Produk Segar Dan Matang Dari Ikan Nila (Oreochromis niloticus) Hasil Pengawetan Menggunakan Daun Keluwek (Pangium Edule Reinw.)

Dayak tribe, as the native people of Kalimantan, has used the Keluwek plant for generations to prepare food sources of protein such as meat and fish. Apart from getting a preferred taste, this plant has also extended the food shelf life. This research aims to determine the sensory response profile of cooked products from preserved fish using Keluwek leaves. Tilapia fish is preserved using chopped Keluwek leaves for 2 days, then steamed and fried to get the cooked product. The organoleptic properties of fresh fish from preservation were compared with fresh fish without preservation using a quality assessment score of 1-9 based on SNI 01-2729.1-2006 for eyes, body surface mucus, odor, and texture. The cooked product's hedonic organoleptic properties and hedonic quality (color, aroma, taste and texture) were also observed (score 1-5). The results showed that on day 2 of preservation, the sensory properties of preserved fish were significantly different (p0.001) from fish without preservation for the eye, body surface mucus, smell, and texture attribute. Meanwhile, the sensory properties of the steamed cooked product are significantly different (p0.001) from those for the taste attribute but are not significantly different (p0.001) for the color, aroma, and texture attribute. Likewise, the sensory properties of the cooked fried product are significantly different (p0.001) for the color, aroma, taste and texture attribute. The steamed cooked product of preserved tilapia received a neutral response. for the attribute of color, aroma, taste, and texture. Meanwhile, the cooked fried product of preserved tilapia received a bit dislike response for the attribute of color, smell, taste, and a neutral response to texture attribute.Keywords: Keluwek, Nile fish, fish preservation.

Formulation and antibacterial activity of frankincense sap extract deodorant spray (Styrax benzoin)

Deodorant is a cosmetic preparation used by the wider community to control and reduce body odor. Deodorant act as antibacterial and are able to reduce the excretion of sweat in the armpits. Frankincense is a plant that contains flavonoids and tannins can act as antibacterial. This aim of this study was to determine the effectiveness of frankincense sap extract as an antibacterial in deodorant spray preparation. Frankincense sap powder is macerated with ethanol to obtain frankincense sap extract. Deodorant formulations are prepared from frankincense sap extract in various concentrations (5%, 10%, 15%, 20%, 25%) and combined with cosolvent, emollient, and solvent. Tests carried out include antibacterial testing against Pseuodomonas aureugenisa and Staphylococcus epidermidis bacteria, organoleptic test, pH test, viscosity test, and irritation test. The test results showed that antibacterial testing gave the highest inhibitory diameter of 2.63 mm and 5.35 mm which was exhibited by formulation 5.

Morphology Profile, Anatomy and Phytochemistry of Beluntas Leaves (Pluchea indica)

Beluntas (Pluchea indica), is one of species in Asteraceae family, traditionally used to increase appetite, to treat indigestion, rheumatism and body odor problems. This study aims to provide morphological and anatomical profiles of Beluntas leaves which are often used in medicine, to determine the chemical content of Beluntas leaves using phytochemical screening tests and thin layer chromatografic profiles, and also to determine flavonoids, polyphenols and alkaloids contents. The observation results showed Beluntas leaves are an oval leaf shape with pinnate leaf reinforcement. Anatomical observation of the leaves, found that the leaf type is equifacial with collateral vascular bundles, the presence of non glandulair multicellular trichomes with constricted in the cross wall, papillae and glandular Compositae trichomes. Tube method results and thin layer chromatography profiles showed Beluntas leaves contained polyphenols, saponins, alkaloids, flavonoids, steroids and essential oils. The chromatogram profile with infrared spectrophotometry shows the wave numbers in the fingerprint region 1515 – 1516, 1259 – 1260, 1159 – 1162, 1112 – 1115, 1046 – 1048, 811 – 812, 505 – 506 and 452 – 454 cm-1. The total amounts of polyphenols, flavonoid and alkaloid contained in Beluntas leaves were 3.1 - 7.7% w/w, 0.5 - 1.2% w/w and 0.03 – 0.08% w/w, respectively.

From Beauty to Identity: Cultural Value Betangas Tradition in Sambas Malay Society

The purpose this research is to determine the significance of the Betangas tradition's values, meanings, and processions among the Sambas Malay population in West Kalimantan province. The examine method used is a qualitative research method with a descriptive research type. The examine approach employed is qualitative using a descriptive study design. The Betangas tradition is generally carried out when there is a wedding celebration, especially in the Sambas area. The purpose of the Betangas tradition is being replaced by grooming equipment for the bride and groom when approaching the wedding. This is because Betangas have the goal of reducing excessive body odor and cleansing the body of both the bride and groom who will carry out the wedding and the special assumptions of the Sambas people. This study obtained results in the meaning of Betangas as a form of gratitude to Allah Subhanahu Wa Ta'alla for the Sambas Malay community, who are mostly Muslim and as a request for the two bride and groom to live a household life. While the function of the Betangas procession is to reduce sweating and cleanse the body's unpleasant odor caused by human sweat. Generally, the Betangas tradition is carried out for the bride and groom, but it is possible that anyone can carry out this tradition. The Betangas tradition is carried out from generation to generation and is still strong in the Sambas region in the Sambas Malay community as local wisdom that is still alive amidst the currents of globalization.

ANTIBACTERIAL ACTIVITY OF BASIL LEAVES EXTRACT TOWARDS BACTERIA STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCUS EPIDERMIDIS IN DEODORANT SPRAY

Body odor is a significant problem and is often encountered in some individuals characterized by excessive odor. A deodorant spray made from basil leaf (Ocimum Basilicum L.) extract is a product used to treat body odor caused by Staphylococcus Aureus and Staphylococcus epidermidis bacteria. The purpose of this study is to assess the efficacy of deodorant spray and basil leaf extract on bacterial activity, as well as the optimal dose of deodorant spray for lowering the activity of these bacteria. Method the research was conducted experimentally, testing antibacterial activity using agar diffusion. The results of the deodorant spray preparation test showed that the preparation was homogeneous, organoleptically brown in color, had a distinctive smell, and had a liquid texture; the pH test results met the requirements for deodorant spray 4.5-6.5. The average diameter of the inhibition zones produced from each preparation were Staphylococcus epidermidis bacteria, positive control 7 mm, negative control 10.1 mm; formula 5% 12.3 mm; formula 10% 13.3 mm; formula 15% 12.7 mm maximum concentration in inhibiting bacteria is formula 10% by 13.3 mm, while the bacteria Staphylococcus Aureus positive control 13 mm, negative control 11.2 mm, 5% formula 13.1 mm; formula 10% 13.4 mm; 15% formula 13.9 mm maximum concentration in inhibiting bacteria is 15% formula 13.9 mm. Based on the inhibitory zone created by 10% basil leaf ethanol extract, Staphylococcus epidermidis bacteria had a 13.3 mm clean zone and Staphylococcus aureus bacteria had a 26.5 mm clean zone.