Background: The problem with a night shift working at that, the human race is diurnal, who are functions during day time. Night workers report a number of health problems. Therefore these negative effects have consequences not just for individual, but also for work place, as decreased alertness and reduced job performance that could endanger human lives and affect the quality of life. The assumption is that ergonomically designed shift schedules will assist in the reduction of physiological and psychosocial hazards in the workplace. Objective: This study was aimed to find out the factors influencing night shift of nurses working in Shahid Ziaur Rahman Medical College Hospital, Bogra Methods: A descriptive study was used in this study. Data was collected using semi-structured questionnaire and the data was analyzed using SPSS. Results: Here, 16% of the respondents felt discomfort during night shift. 66% they don’t get enough sleep after night duty. 60% of the respondents mentioned that night shift sometimes affects their attitude negatively. 70% stated that night shift sometimes affects their mood. 70% also stated that night shift sometimes disturbs their social life. 80% mentioned that night shift sometimes increases interpersonal conflict in their family. Conclusion: The findings revealed that the participated nurses were not comfortable with night shift at all. Night workers accumulate a sleep deficit and sleepy workers make mistakes. The most dangerous are those who build up a “sleep debt” depriving themselves of sleep over long periods.