Broader Strategy Research Articles

Youth Engagement in Transforming the Food System to Address Malnutrition in the Philippines

Unless the Philippine food system is made more resilient, the aspirations of AmBisyon Natin 2040 will remain elusive, hindering the country’s ability to achieve inclusive growth, eradicate poverty, and ensure the well-being of all Filipinos. Focused on the youth, this paper brings together three related issues: (1) how can the youth be encouraged to devote their careers to agriculture? (2) How do poor quality diets compromise the cognitive abilities of young people? and (3) How can nutrition concerns be incorporated into agricultural policies? Early exposure to agricultural concepts can inspire young people to pursue careers in agriculture, ensuring a steady influx of leaders and professionals equipped to tackle the challenges within the food system. This approach not only secures the future of the agriculture sector but also promotes innovation and resilience in food production and distribution. One in two preschoolers in the lowest wealth quintile in the Philippines are stunted, while only one in eight in the highest wealth quintile are stunted. Children who are stunted are not reaching the full potential of their cognitive development. The dietary quality of mothers before pregnancy, during pregnancy, and during breastfeeding improves markedly with income and so is associated with the better heights of their children. In the long term, agricultural productivity must be improved with a particular focus on increasing the incomes of the poor. Increasing household income is the most sustainable way to improve dietary quality. In the shorter term, there are two broad strategies to link Philippine agriculture to nutrition by adding more minerals and vitamins to the food supply: (1) by increasing the density of minerals and vitamins in food staples, a strategy referred to as “biofortification,” and (2) by lowering the prices of nutritious nonstaple food such as vegetables, fruits, pulses, fish, and meat through improved productivity that results in rapid supply increases.

How to make cardiology clinical trials more inclusive.

Cardiovascular clinical trials continue to under-represent children, older adults, females and people from ethnic minority groups relative to population disease distribution. Here we describe strategies to foster trial representativeness, with proposed actions at the levels of trial funding, design, conduct and dissemination. In particular, trial representativeness may be increased through broad recruitment strategies and site selection criteria that reflect the diversity of patients in the catchment area, as well as limiting unjustified exclusion criteria and using pragmatic designs that minimize research burden on patients (including embedded and decentralized trials). Trial communications ought to be culturally appropriate; engaging diverse people with lived experience in the co-design of some trial elements may foster this. The demographics of trialists themselves are associated with participant demographics; therefore, trial leadership must be actively diversified. Funding bodies and journals increasingly require the reporting of sociodemographic characteristics of trial participants, and regulatory bodies now provide guidance on increasing trial diversity; these steps may increase the momentum towards change. Although this Perspective focuses on the cardiovascular trial context, many of these strategies could be applied to other fields.

Pharmacy benefit managers and drug affordability policy proposals for enhancing access in underserved communities

Pharmacy Benefit Managers (PBMs) play a pivotal role in the pharmaceutical supply chain, yet their practices have increasingly contributed to the rising costs of prescription drugs and limited access to essential medications, particularly in underserved communities. This paper critically examines the role of PBMs, highlighting their challenges to drug affordability and accessibility. It explores the limitations of existing policies and proposes comprehensive reforms to enhance transparency, accountability, and fairness in PBM operations. The proposed policy interventions include mandatory transparency in PBM rebates and formularies, regulations on rebate retention, and fair reimbursement rates for pharmacies. Additionally, broader strategies such as government regulation of drug prices, expanded subsidies for low-income populations, and value-based contracts are discussed as potential solutions. The paper concludes with recommendations for policymakers, healthcare providers, and PBMs, emphasizing the need for collaborative efforts to ensure the successful implementation of these reforms. Addressing the issues posed by current PBM practices, these policy proposals aim to significantly improve drug affordability and access in underserved communities, ultimately contributing to a more equitable healthcare system. Keywords: Pharmacy Benefit Managers (PBMs), Drug Affordability, Healthcare Access, Underserved Communities, Policy Reform, Pharmaceutical Supply Chain.

‘Burnt out by all the exploitation’: Involuted labour in creative fields

This article develops a neo-Bourdieusian analysis of how creative workers respond to the pressures of freelance, contract and project-based cultural production. Using data from 15 in-depth interviews with independent ‘creatives’ we enrol Bourdieu's fields and Clifford Geertz's account of involution – internal over-elaboration – to investigate the tendencies, nuances and outcomes of creative labour's production practices. To answer the field's piecemeal funding and limited market rewards three broad strategic involution strategies are outlined: ‘hard driving’ of resources; reworking and hybridization of existing cultural forms; and uncodified intricacy within the social relations of production. This theorization illustrates how iterative internal complexity aids the economizing of cultural production oriented to gaining recognition while, due to the social intricacy of creative processes, inducing a tendency towards emotional and physical fatigue. Rather than promote a meritocratic productivity imagined by policy discourses, involuted labour produces burnout and thus contributes to the churn of creative talent.

Understanding patient and health care provider perceptions of barriers and facilitators to diabetes care in immigrant and refugee populations

BackgroundThere is a lack of literature which assesses both patient and health care provider (HCP) perceptions of barriers and facilitators to diabetes health care in immigrant and refugee populations within the United States. This study uniquely incorporates both cohorts’ perceptions on the same topic to assess for congruency and discrepancies in perspectives.MethodsSurveys were deployed to patients with diabetes and HCPs to solicit perceptions of barriers and facilitators to diabetes care, with the intention to determine similarities and differences between cohorts. A literature review was conducted to explore barriers and facilitators to diabetes care in immigrant/refugee populations in the US to aid in development of the survey used in this study, as no validated survey existed. Broad recruitment strategies were used, including snowball sampling. Descriptive statistics were calculated and reported p-values were derived from a chi-squared or Fisher’s exact test and used for categorical data and a Wilcoxon rank sum or Kruskal–Wallis rank sum test was used for continuous data. Qualitive analysis was completed using the inductive analysis method.ResultsAmong 19 immigrant and refugee patients and 35 HCPs, almost all facilitators to diabetes care were endorsed by patients and HCPs as helpful. The highest ranked facilitator among patients and HCPs was having access to health insurance. Patients rated stress, while HCPs rated lack of health insurance as the biggest barriers to diabetes care for immigrants and refugees. Patients and HCPs differed in perspective on who held primary responsibility for diabetes management. Patients wanted more diabetes education and nutrition support, while HCPs thought understanding patients’ cultural context/sensitivity, providing more education, and having language/translation services were the most important things HCPs can do to care for patients’ diabetes.ConclusionsImmigrant/refugee patients and HCPs largely agree about what factors are most helpful in managing diabetes, with access to health insurance as the greatest facilitator. However, while HCPs identified lack of health insurance as the greatest barrier to care, immigrant and refugee patients cited the role of stress as the greatest challenge in managing their diabetes. There was a striking difference in perception of responsibility for diabetes management, with 89% of patient participants responding that the patient is responsible, while only 17% of HCPs responded that the patient was responsible. Future work should include surveys translated into languages other than English to further elicit the perspective of diabetes care in immigrant/refugee populations. Clinical recommendations based on this study include assessing and supporting patient stressors, assuring patients understand that diabetes management is a partnership, providing culturally tailored diabetes education, and increasing access to health insurance.

A Study on the Relationship of Business Intelligence and Business Performance Management

In order for corporations to attach their operational and strategic targets with everyday activities, Business Performance Management (BPM) is a important challenge. By making it simpler to make educated decisions and provide useful data, this lets in for comprehensive overall performance management. Not most effective should an enterprise version and track its strategies for BPM to paintings, however it have to additionally model and tune its broader strategies and the assumptions that underpin those plans. Examining the methods, gear, and approaches that make up Business Process Management (BPM) is the number one goal of this newsletter. Along with providing a radical framework that integrates BI and company overall performance control, it will also check out the intrinsic relationship between BPM and BI. This all-encompassing technique to managing agency performance ensures that operational execution and strategic objectives are always in sync by means of promoting continuous improvement and adaptable plans via records-pushed analysis.

Abstract P308: High Prevalence of Secondary Hypertension with Prospective Systematic Screening

Introduction: A systematic screening of hypertensive patients for secondary arterial hypertension (SHT) is generally held to be not justified by the low prevalence of SHT but improved knowledge and diagnostic tools might change this view. Hypothesis: To challenge the notion that the prevalence of SHT is low, we prospectively submitted consenting hypertensive patients presenting between 2017 and 2023 to a systematic search for SHT. Methods: The systematic search for SHT entailed, besides history and full physical examination, renal Doppler ultrasound assessment, a sleep questionnaire, and measurement of serum Na + , K + , ionized Ca 2+ , plasma active renin (DRC), aldosterone concentration (PAC), the aldosterone-renin ratio (ARR), PTH, TSH, and 24-h urinary/plasma Na + , K + , Ca 2+ , phosphate, and metanephrine and normetanephrine excretion. Cure or prominent improvement of hypertension after target treatment was used to confirm SHT. Results: In 494 consecutive patients (median age 62 years, 50% women) who completed the work-up, we found that 11.5% of these patients had isolated systolic hypertension. The overall prevalence of SHT among those with systo-diastolic HT was 72.3%: 25.5% had primary aldosteronism; 13.4% obstructive sleep apnea, 7.9% hypothyroidism, 6.5% renovascular hypertension and 19.0% rarer causes of SHT. Of all patients, only 14% were referred by their general practitioner or other specialists and 85% were self-presenting. Conclusions: Only a small minority of consecutive hypertensive patients seen in a specialized center were referred by their doctors, thus calling for implementation of broad strategies to increase the awareness of SHT. The fact that a systematic screening showed that SHT is far more common than currently believed supports this conclusion.

Hjernetegn.dk-The Danish Central Nervous System Tumor Awareness Initiative Digital Decision Support Tool: Design and Implementation Report.

Childhood tumors in the central nervous system (CNS) have longer diagnostic delays than other pediatric tumors. Vague presenting symptoms pose a challenge in the diagnostic process; it has been indicated that patients and parents may be hesitant to seek help, and health care professionals (HCPs) may lack awareness and knowledge about clinical presentation. To raise awareness among HCPs, the Danish CNS tumor awareness initiative hjernetegn.dk was launched. This study aims to present the learnings from designing and implementing a decision support tool for HCPs to reduce diagnostic delay in childhood CNS tumors. The aims also include decisions regarding strategies for dissemination and use of social media, and an evaluation of the digital impact 6 months after launch. The phases of developing and implementing the tool include participatory co-creation workshops, designing the website and digital platforms, and implementing a press and media strategy. The digital impact of hjernetegn.dk was evaluated through website analytics and social media engagement. hjernetegn.dk was launched in August 2023. The results after 6 months exceeded key performance indicators. The analysis showed a high number of website visitors and engagement, with a plateau reached 3 months after the initial launch. The LinkedIn campaign and Google Search strategy also generated a high number of impressions and clicks. The findings suggest that the initiative has been successfully integrated, raising awareness and providing a valuable tool for HCPs in diagnosing childhood CNS tumors. The study highlights the importance of interdisciplinary collaboration, co-creation, and ongoing community management, as well as broad dissemination strategies when introducing a digital support tool.

Trade war: the steel and aluminum markets and the trade governance

The international institutionalism has been under debate. The World Trade Organization (WTO) has not been immune to strong criticisms that has been exacerbated in recent years. A turning point in this process was the trade war between the United States and China that began in 2018. It was characterized by tariff increases that persist. The contribution of this work is to show how these measures taken by relevant global players (such as the United Sates and China) have significantly affected the international markets and it impacted on third countries, not only in terms of trade flows but also when considering the international trade regulatory framework. The drop in the demand from the United States implied a fall in the global demand that caused a fall in international prices. Moreover, it is shown that Trump's measures were successful given that China was substituted by other suppliers. Finally, his protectionist strategy against China, was accompanied by a broader strategy against the World Trade Organization by blocking not only the negotiations during the Buenos Aires Ministerial Conference but also the Dispute Settlement System.

A comprehensive molecular phylogeny of Cephalotrichum and Microascus provides novel insights into their systematics and evolutionary history.

The genera Cephalotrichum and Microascus contain ecologically, morphologically and lifestyle diverse fungi in Microascaceae (Microascales, Sordariomycetes) with a world-wide distribution. Despite previous studies having elucidated that Cephalotrichum and Microascus are highly polyphyletic, the DNA phylogeny of many traditionally morphology-defined species is still poorly resolved, and a comprehensive taxonomic overview of the two genera is lacking. To resolve this issue, we integrate broad taxon sampling strategies and the most comprehensive multi-gene (ITS, LSU, tef1 and tub2) datasets to date, with fossil calibrations to address the phylogenetic relationships and divergence times among major lineages of Microascaceae. Two previously recognised main clades, Cephalotrichum (24 species) and Microascus (49 species), were re-affirmed based on our phylogenetic analyses, as well as the phylogenetic position of 15 genera within Microascaceae. In this study, we provide an up-to-date overview on the taxonomy and phylogeny of species belonging to Cephalotrichum and Microascus, as well as detailed descriptions and illustrations of 21 species of which eight are newly described. Furthermore, the divergence time estimates indicate that the crown age of Microascaceae was around 210.37 Mya (95 % HPD: 177.18-246.96 Mya) in the Late Triassic, and that Cephalotrichum and Microascus began to diversify approximately 27.07 Mya (95 % HPD: 20.47-34.37 Mya) and 70.46 Mya (95 % HPD: 56.96-86.24 Mya), respectively. Our results also demonstrate that multigene sequence data coupled with broad taxon sampling can help elucidate previously unresolved clade relationships. Citation: Wei TP, Wu YM, Zhang X, et al. 2024. A comprehensive molecular phylogeny of Cephalotrichum and Microascus provides novel insights into their systematics and evolutionary history. Persoonia 52: 119-160. https://doi.org/10.3767/persoonia.2024.52.05 .

Strategic familismo: how Hispanic/Latine students negotiate family values and their stem careers

Hispanic/Latine young adults remain severely under-represented in STEM fields. While structural barriers contributing to these disparities have been thoroughly examined in previous research, the current study brings focus to the strategies young adults employ to navigate the incongruence between the cultures of higher education and STEM and the cultural value of “familismo,” a central orientation in Latin American cultures that prioritizes family ties over the individual. This study reports findings from 19 interviews and two focus groups we conducted with Hispanic/Latine young adults who had been part of an undergraduate STEM program. Focusing on participants’ first two years after college graduation, we identify the strategies these young adults employed to combine familismo with their goals for successful careers in STEM related fields. We identify three broad strategies, strategic selection, strategic timing, and strategic bridging; which enables Hispanic/Latine young adults to cultivate successful careers in STEM without rejection of their home-based cultural values. We also explore how participants’ perspectives and strategies shifted with the onset of the COVID-19 pandemic. Our findings contribute to a growing interest in recognizing how Hispanic/Latine young adults exercise agency to successfully negotiate structural and cultural barriers in the pursuit of STEM careers.

Preface

This WSBE24 Conference Proceedings comprise the selected papers presented at the 2024 World Sustainable Built Environment Conference (WSBE24). The world conference, held on a triennial basis, is the premier event of the Sustainable Built Environment (SBE) Conference Series managed by the SBE Partners and Permanent Co-hosts – consisting of the International Initiative for a Sustainable Built Environment (iiSBE), the International Council of Research and Innovation in Building and Construction (CIB), the International Federation of Consulting Engineers (FIDIC) and the UN Environment Programme (UNEP). WSBE24 was conducted online from 12-14 June 2024, under the overarching theme of “Innovation Pathways & Partnerships for Sustainable Built and Urban Environments”. This conference not only facilitated a diverse array of talks and discussions but also ensured wide accessibility by publishing its proceedings as an open access document, with both the whole proceedings and the individual papers available online. The video recordings of the presentations are also available online in another platform. This Proceedings include the articles that underpin the conference’s scientific program. Each submission underwent a double-blind peer-review process. A globally represented Scientific Committee and the Editors carefully reviewed initial abstracts and full paper submissions, ensuring that only those meeting the necessary scientific standards were presented at the conference and included in these proceedings. The breadth of topics covered in these papers is vast, spanning current trends and innovations, challenges and solutions, and empirical findings across the fields of science, industry, policy, and governance. A major focus was placed on reducing greenhouse gas emissions within the construction, building and real estate sector, reflecting discussions in line with those at the Buildings and Climate Global Forum held in Paris in March 2024, organized by UNEP and the French Government, amongst others. This collection reflects the field’s most pressing challenges and emerging solutions. Papers examine greenhouse gas reduction strategies, the integration of climate action and resource conservation, and the transition toward a circular economy. Tools for environmental performance assessment – including the expanding role of lifecycle assessment (LCA) in design and management workflows, especially in a digital environment (e.g., BIM) – feature prominently. Questions and innovative research and solutions range from specific materials and building physics in individual buildings to broad urban development strategies, always guided by the principles of sustainable development. The continuing needs for enhanced educational and capability development – which is also an emerging subject within the Global Alliance for Building and Construction (GlobalABC) – and expanded scope of SBE issues and impacts on people and communities and economics, and vice versa, in future editions are highlighted. The first principles concept of sustainability continues to be a suitable umbrella for topics such as circularity, resilience and regenerative design. List of Reviewers is available in this Pdf.

Lewis Acid-Driven Inverse Hydride Shuttle Catalysis.

Inverse hydride shuttle catalysis provides a multicomponent platform for the highly efficient synthesis of alkaloid frameworks with exquisite diastereoselectivity. However, a number of limitations hinder this method, primarily the strict requirement for highly electron-deficient acceptors. Herein, we present a general Lewis acid-driven approach to address this constraint, and have developed two broad strategies enabling the modular synthesis of complex azabicycles that were entirely unattainable using the previous method. The enhanced synthetic flexibility facilitates a streamlined asymmetric cyclization, leading to a concise total synthesis of the alkaloid (-)-tashiromine.

Lewis Säure‐Getriebene Inverse Hydrid‐Shuttle Katalyse

AbstractInverse Hydrid‐Shuttle Katalyse bildet eine Plattform für die hocheffiziente und diastereoselektive Synthese von Alkaloidstrukturen durch Multikomponentenreaktionen. Mehrere Einschränkungen hindern jedoch die breite Anwendbarkeit dieses Ansatzes, darunter vor allem die Notwendigkeit nach besonders elektronenarmen Akzeptoren. Hier präsentieren wir einen allgemeinen, durch Lewis Säuren getriebenen Ansatz, um diese Einschränkung anzugehen, und haben zwei Strategien entwickelt, die die modulare Synthese komplexer azabicyclischer Systeme ermöglichen, die mit der früheren Methode unerreichbar waren. Die so gewonnene synthetische Flexibilität führt zu einer verbesserten asymmetrischen Cyclisierung, die eine kurze Totalsynthese des Alkaloids (−)‐Tashiromin ermöglicht hat.

Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy

Total tumor clearance through immunotherapy is associated with a fully coordinated innate and adaptive immune response, but knowledge on the exact contribution of each immune cell subset is limited. We show that therapy-induced intratumoral CD8+ Tcells recruited and skewed late-stage activated M1-like macrophages, which were critical for effective tumor control in two different murine models of cancer immunotherapy. The activated CD8+ Tcells summon these macrophages into the tumor and their close vicinity via CCR5 signaling. Exposure of non-polarized macrophages to activated Tcell supernatant and tumor lysate recapitulates the late-stage activated and tumoricidal phenotype invitro. The transcriptomic signature of these macrophages is also detected in a similar macrophage population present in human tumors and coincides with clinical response to immune checkpoint inhibitors. The requirement of a functional co-operation between CD8+ Tcells and effector macrophages for effective immunotherapy gives warning to combinations with broad macrophage-targeting strategies.

Risk mitigations to prevent methotrexate dispensing errors in English hospitals: a mixed-methods exploratory study

Abstract Introduction Oral methotrexate is indicated for paediatric cancer indications and autoimmune diseases. Unfortunately, cases of inadvertent overdose due to confusion between 2.5mg and 10mg tablet strengths are widely documented. National guidance is available to prevent such errors; indeed, the overdose of methotrexate for non-cancer treatment is a Never Event.[1] At our organisation, the main mitigations to reduce the risk of dispensing errors were to segregate tablet strengths and restrict 10mg tablets to cancer indications.[2] Adherence had not been evaluated, and alternative risk mitigations had not been explored. Aim We aimed to 1) determine whether methotrexate 10mg tablets were restricted to cancer indications; and 2) explore strategies to prevent errors associated with methotrexate tablet strength at a sample of hospitals. Methods First, an audit approach was used to determine use of 10mg tablets; standards were derived from local policy.[2] The setting was a large teaching hospital providing acute, specialist, paediatric and cancer care. Dispensing transactions of 10mg tablets on the electronic pharmacy dispensing system between April 2022 and March 2023 were included. Details of prescription, dispensed quantity, dose instructions and speciality were recorded onto the data collection tool. Data were analysed descriptively. Second, a survey was conducted with a sample of twenty English hospitals (including the study site). These comprised ten large teaching hospitals and specialist paediatric cancer centres. A relevant pharmacist was contacted by email at each trust and asked a series of open questions about local risk strategies associated with methotrexate 10mg tablets. Notes were taken and data summarised descriptively. Results There were 79 dispensing issues for methotrexate 10mg tablets. Of those, 65 issues were for 13 cancer patients; the other 14 were erroneous issues where 10mg were dispensed instead of 2.5mg for non-cancer indications. Whilst most errors were rectified (n=13), one patient received a weekly dose of 40mg instead of 10mg, a four-times overdose. Nineteen (95%) trusts responded to our survey. There were three broad strategies reported: to only use 2.5mg tablets (n=9); isolate dispensing of 10mg tablets to dedicated cancer or paediatric dispensaries (n=5); or reliance on local protocol and physical segregation of 2.5mg and 10mg tablets within multi-service dispensaries (n=5). Conclusion It was encouraging that 10mg tablets were solely intended for cancer indications. However, the incidental discovery of errors highlighted the need for improvement. Findings suggest that eliminating 10mg tablets from practice was a feasible strategy, even for organisations providing cancer services. Findings have limited generalisability due to the small sample sizes, and detail provided in survey responses was variable. Nevertheless, the audit was effective in identifying a previously unknown Never Event. National Health Service organisations should acknowledge the risk associated with keeping 2.5mg and 10mg methotrexate tablets, particularly within dispensaries servicing multiple specialties. Dedicated cancer dispensaries are not commonplace, therefore eliminating 10mg tablets altogether should be considered. Indeed, the principle of limiting strengths is a well-established and effective medication risk management strategy. Further research should explore the potential negative implications for patients, such as increased tablet burden, if 2.5mg tablets are used exclusively for all indications. This may further inform national strategy to prevent Never Events.

Inhibition of Pim kinases triggers a broad antiviral activity by affecting innate immunity and via the PI3K-Akt-mTOR axis the endolysosomal system

Zoonoses such as ZIKV and SARS-CoV-2 pose a severe risk to global health. There is urgent need for broad antiviral strategies based on host-targets filling gaps between pathogen emergence and availability of therapeutic or preventive strategies. Significant reduction of pathogen titers decreases spread of infections and thereby ensures health systems not being overloaded and public life to continue. Based on previously observed interference with FGFR1/2-signaling dependent impact on interferon stimulated gene (ISG)-expression, we identified Pim kinases as promising druggable cellular target. We therefore focused on analyzing the potential of pan-Pim kinase inhibition to trigger a broad antiviral response.The pan-Pim kinase inhibitor AZD1208 exerted an extraordinarily high antiviral effect against various ZIKV isolates, SARS-CoV-2 and HBV. This was reflected by strong reduction in viral RNA, proteins and released infectious particles. Especially in case of SARS-CoV-2, AZD1208 led to a complete removal of viral traces in cells. Kinome-analysis revealed vast changes in kinase landscape upon AZD1208 treatment, especially for inflammation and the PI3K/Akt-pathway. For ZIKV, a clear correlation between antiviral effect and increase in ISG-expression was observed. Based on a cell culture model with impaired ISG-induction, activation of the PI3K-Akt-mTOR axis, leading to major changes in the endolysosomal equilibrium, was identified as second pillar of the antiviral effect triggered by AZD1208-dependent Pim kinase inhibition, also against HBV.We identified Pim-kinases as cellular target for a broad antiviral activity. The antiviral effect exerted by inhibition of Pim kinases is based on at least two pillars: innate immunity and modulation of the endolysosomal system.

How do people use reappraisal? An investigation of selection frequency and affective outcomes of reappraisal tactics.

Although the effects of different emotion regulation strategies are well-documented, most studies to date have focused on the selection and implementation of broad strategies, while overlooking the selection and implementation of specific tactics to enact those strategies. The present research investigated the strategy of cognitive reappraisal and the differences in selection frequency and affective outcomes that are associated with the implementation of different reappraisal tactics to enact that strategy. Participants completed a laboratory task in which they were instructed to reappraise or not to reappraise negative images and reported on their use of specific reappraisal tactics for every trial. Using established reappraisal tactic coding, we assessed how people selected from among common tactics for each image (Study 1) and all tactics (Study 2) and implemented those tactics to reappraise negative images. We compared reappraisal tactic selection and implementation when used during instructed reappraisal versus during spontaneous reappraisal, in the nonreappraise condition. Results of both studies indicate that tactics were used more often when instructed to reappraise versus when spontaneously reappraising. Participants used some tactics (e.g., reality challenge) more frequently compared to the rest of the tactics in both conditions. Negative affect was lower following instructed versus spontaneous reappraisal. Some tactics (e.g., change current circumstances) were more effective at decreasing negative affect in both conditions. Knowing which reappraisal tactics are most frequently selected, and their affective outcomes when used when prompted or spontaneously, may help us better understand how to improve people's ability to use reappraisal to achieve their emotional goals. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Escherichia coli Cytoplasmic Expression of Disulfide-Bonded Proteins: Side-by-Side Comparison between Two Competing Strategies.

The production of disulfide bond-containing recombinant proteins in Escherichia coli has traditionally been done by either refolding from inclusion bodies or by targeting the protein to the periplasm. However, both approaches have limitations. Two broad strategies were developed to allow the production of proteins with disulfide bonds in the cytoplasm of E. coli: i) engineered strains with deletions in the disulfide reduction pathways, e.g. SHuffle, and ii) the co-expression of oxidative folding catalysts, e.g. CyDisCo. However, to our knowledge, the relative effectiveness of these strategies has not been properly evaluated. Here, we systematically compare the purified yields of 14 different proteins of interest (POI) that contain disulfide bonds in their native state when expressed in both systems. We also compared the effects of different background strains, commonly used promoters, and two media types: defined and rich autoinduction. In rich autoinduction media, POI which can be produced in a soluble (non-native) state without a system for disulfide bond formation were produced in higher purified yields from SHuffle, whereas all other proteins were produced in higher purified yields using CyDisCo. In chemically defined media, purified yields were at least 10x higher in all cases using CyDisCo. In addition, the quality of the three POI tested was superior when produced using CyDisCo.

A collaborative network trial to evaluate the effectiveness of implementation strategies to maximize adoption of a school-based healthy lunchbox program: a study protocol.

An important impediment to the large-scale adoption of evidence-based school nutrition interventions is the lack of evidence on effective strategies to implement them. This paper describes the protocol for a "Collaborative Network Trial" to support the simultaneous testing of different strategies undertaken by New South Wales Local Health Districts to facilitate the adoption of an effective school-based healthy lunchbox program ('SWAP IT'). The primary objective of this study is to assess the effectiveness of different implementation strategies to increase school adoption of the SWAP across New South Wales Local Health Districts. Within a Master Protocol framework, a collaborative network trial will be undertaken. Independent randomized controlled trials to test implementation strategies to increase school adoption of SWAP IT within primary schools in 10 different New South Wales Local Health Districts will occur. Schools will be randomly allocated to either the intervention or control condition. Schools allocated to the intervention group will receive a combination of implementation strategies. Across the 10 participating Local Health Districts, six broad strategies were developed and combinations of these strategies will be executed over a 6 month period. In six districts an active comparison group (containing one or more implementation strategies) was selected. The primary outcome of the trial will be adoption of SWAP IT, assessed via electronic registration records captured automatically following online school registration to the program. The primary outcome will be assessed using logistic regression analyses for each trial. Individual participant data component network meta-analysis, under a Bayesian framework, will be used to explore strategy-covariate interactions; to model additive main effects (separate effects for each component of an implementation strategy); two way interactions (synergistic/antagonistic effects of components), and full interactions. The study will provide rigorous evidence of the effects of a variety of implementation strategies, employed in different contexts, on the adoption of a school-based healthy lunchbox program at scale. Importantly, it will also provide evidence as to whether health service-centered, collaborative research models can rapidly generate new knowledge and yield health service improvements. This trial is registered prospectively with the Australian New Zealand Clinical Trials Registry (ACTRN12623000558628).