An extensive experimental database comprising air–water as well as steam-water upwards vertical pipe flows for a pressure up to 6.5 MPa was used to investigate the effect of the lateral lift force on turbulent poly-dispersed flows with medium or high gas volume fraction. It was clearly shown that the lift force plays an important role also in such flows. Several effects such as bubble coalescence and breakup as well as fast rising large bubbles which push small bubbles towards the pipe wall superpose the effect of the lift force but can be separated from this effect. The critical bubble diameter, at which the lift force changes its sign, predicted by using Tomiyama’s correlation agrees well with experimental data obtained for turbulent air–water and steam-water flows with medium and high void fraction and a broad spectrum of bubbles sizes. The values for this critical bubble diameter are confirmed by the experimental data within the frame of the uncertainty of the data. Consequences of the action of the lateral lift force on flow structures in different flow situations are discussed. From the investigations it can be concluded that the lift force including the bubble size dependent change of its sign should be considered in a proper numerical 2D or 3D-simulation on flows in which bubbles in the range of several millimeters are present.