The aim of this study was to establish normal ophthalmic parameters for selected diagnostic tests, and to describe the orbital anatomy and adnexal histology of the broad-snouted caiman. A total of 35 Caiman latirostris that were free of obvious ocular diseases were used to measure the parameters in this investigation. Ages ranged from 5 to 15years. Ophthalmic diagnostic tests were conducted, including evaluation of tear production with Schirmer Tear test-1 (STT1), culture of the conjunctival bacterial flora, applanation tonometry, conjunctival cytology, nictiating membrane incursion frequency test (NMIFT), endodontic absorbent paper point tear test (EAPPTT), palpebral fissure length measurement (PFL) and B-mode ultrasonography. Adnexal histology and skull samples were studied. Mean (±SD) STT1 was 3.4±3.6mm/min (95% confidence interval of 2.01-4.78mm/min), intraocular pressure (IOP) was 12.9±6.2mmHg, NMIFT was 6.0±3.5, EAPPTT was 17.1±2.5mm/min, PFL was 28.9±3.0mm, anterior chamber depth was 3.1±0.3mm, lens axial length was 8.4±0.6mm, vitreous chamber depth was 7.9±0.7mm and axial globe length was 19.9±1.3mm. For all animals evaluated, Bacillus sp., Diphteroids and Staphylococcus sp. were predominant.