Carbon nanofibers (CNFs) are good candidates for nano-system applications because they have the excellent mechanical and the electrical properties. The mechanical and electrical properties of a single CNF were measured. A tensile test and a measurement of the electrical resistance of CNFs during elongation were performed inside a scanning electron microscope. We confirmed that the CNFs used in this experiment consisted of a polycrystalline structure and an amorphous phase by a result of Raman. Additionally, we observed that the crystal structure in nanofibers exhibits brittle fracture behavior and the amorphous phase make them relatively ductile. The elastic moduli of the CNFs were 9.57 to 13.6 GPa in the elastic section. The electrical resistance of the CNFs exhibited unusual behavior during elongation. The electrical resistance of the CNFs exhibited stable resistance increase like as the tensile results in the initial region. But the electrical resistance exhibited generally irregular increase after initial region because of the polycrystalline structure and amorphous phase. The strain sensitivity of the CNFs exhibited a much lower value.