Bright Spectra Research Articles

Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.

We present a novel approach for deep vascular imaging in rodent cortex at excitation wavelengths susceptible to water absorption using two-photon microscopy with photons of dissimilar wavelengths. We demonstrate that non-degenerate two-photon excitation (ND-2PE) enables imaging in the water absorption window from 1400-1550 nm using two excitation sources with temporally overlapped pulses at 1300 nm and 1600 nm that straddle the absorption window. We explore the brightness spectra of indocyanine green (ICG) and assess its suitability for imaging in the water absorption window. Further, we demonstrate in vivo imaging of the rodent cortex vascular structure up to 1.2 mm using ND-2PE. Lastly, a comparative analysis of ND-2PE at 1435 nm and single-wavelength, two-photon imaging at 1300 nm and 1435 nm is presented. Our work extends the excitation range for fluorescent dyes to include water absorption regimes and underscores the feasibility of deep two-photon imaging at these wavelengths.

Unbiased method for spectral analysis of cells with great diversity of autofluorescence spectra.

Autofluorescence is an intrinsic feature of cells, caused by the natural emission of light by photo-excitatory molecular content, which can complicate analysis of flow cytometry data. Different cell types have different autofluorescence spectra and, even within one cell type, heterogeneity of autofluorescence spectra can be present, for example, as a consequence of activation status or metabolic changes. By using full spectrum flow cytometry, the emission spectrum of a fluorochrome is captured by a set of photo detectors across a range of wavelengths, creating an unique signature for that fluorochrome. This signature is then used to identify, or unmix, that fluorochrome's unique spectrum from a multicolor sample containing different fluorescent molecules. Importantly, this means that this technology can also be used to identify intrinsic autofluorescence signal of an unstained sample, which can be used for unmixing purposes and to separate the autofluorescence signal from the fluorophore signals. However, this only works if the sample has a singular, relatively homogeneous and bright autofluorescence spectrum. To analyze samples with heterogeneous autofluorescence spectral profiles, we setup an unbiased workflow to more quickly identify differing autofluorescence spectra present in a sample to include as "autofluorescence signatures" during the unmixing of the full stained samples. First, clusters of cells with similar autofluorescence spectra are identified by unbiased dimensional reduction and clustering of unstained cells. Then, unique autofluorescence clusters are determined and are used to improve the unmixing accuracy of the full stained sample. Independent of the intensity of the autofluorescence and immunophenotyping of cell subsets, this unbiased method allows for the identification of most of the distinct autofluorescence spectra present in a sample, leading to less confounding autofluorescence spillover and spread into extrinsic phenotyping markers. Furthermore, this method is equally useful for spectral analysis of different biological samples, including tissue cell suspensions, peripheral blood mononuclear cells, and in vitro cultures of (primary) cells.

DESI complete calibration of the colour–redshift relation (DC3R2): results from early DESI data

ABSTRACT We present initial results from the Dark Energy Spectroscopic Instrument (DESI) complete calibration of the colour–redshift relation (DC3R2) secondary target survey. Our analysis uses 230 k galaxies that overlap with KiDS-VIKING ugriZYJHKs photometry to calibrate the colour–redshift relation and to inform photometric redshift (photo-z) inference methods of future weak lensing surveys. Together with emission line galaxies (ELGs), luminous red galaxies (LRGs), and the Bright Galaxy Survey (BGS) that provide samples of complementary colour, the DC3R2 targets help DESI to span 56 per cent of the colour space visible to Euclid and LSST with high confidence spectroscopic redshifts. The effects of spectroscopic completeness and quality are explored, as well as systematic uncertainties introduced with the use of common Self-Organizing Maps trained on different photometry than the analysis sample. We further examine the dependence of redshift on magnitude at fixed colour, important for the use of bright galaxy spectra to calibrate redshifts in a fainter photometric galaxy sample. We find that noise in the KiDS-VIKING photometry introduces a dominant, apparent magnitude dependence of redshift at fixed colour, which indicates a need for carefully chosen deep drilling fields, and survey simulation to model this effect for future weak lensing surveys.

Applying dimensionality reduction methods to extract physiological and diagnostic features for clinical Hyperspectral Images

The most valuable information of Hyperspectral Image (HSI) should be processed properly. Using dimensionality reduction techniques in two distinct approaches, we created a structure for HSI to collect physiological and diagnostic information. The tissue Oxygen Saturation Level (StO2) was extracted using the HSI approach as a physiological characteristic for stress detection. Our research findings suggest that this unique characteristic may not be affected by humidity or temperature in the environment. Comparing the standard StO2 reference and pressure concentrations, the social stress assessments showed a substantial variance and considerable practical differentiation. The proposed system has already been evaluated on tumor images from rats with head and neck cancers using a spectrum from 450 to 900 nm wavelength. The Fourier transformation was developed to improve precision, and normalize the brightness and mean spectrum components. The analysis of results showed that in a difficult situation where awareness could be inexpensive due to feature possibilities for rapid classification tasks and significant in measuring the structure of HSI analysis for cancer detection throughout the surgical resection of wildlife. Our proposed model improves performance measures such as reliability at 89.62% and accuracy at 95.26% when compared with existing systems.

Silver‐Nanoclusters and Vacancies Influence the Optical Properties of Spherical Cs2AgBiCl6 Nanocrystals

AbstractHalide double perovskites (DPs), such as Cs2AgBiX6 (X = Br/Cl), are emerging semiconductors for optoelectronic applications, offering less toxic alternatives compared to lead halide perovskites. Although exhibiting an indirect bandgap, Cs2AgBiCl6 DP nanocrystals (NCs) show bright photoluminescence spectra characterized by a spectrally broad red emission centered ≈650 nm and a narrower blue emission band centered at 425 nm. However, the origin of both emission bands is still under debate. In this paper, it is shown that silver (Ag) plays a crucial role in the explanation of both emission bands observed in these DP NCs. The trapping of holes in Ag vacancies leads to a spatial localization of the hole wave function on the scale of the lattice constant. This provides k‐values for the hole wave function at all boundaries of the Brillouin zone and thus favors the recombination with electrons at the L‐point. Accordingly, a thermally activated behavior of the red photoluminescence with an activation energy of 56 meV is observed. It is further shown that the high‐energetic blue emission band originates from lecithin ligands attached to the NC surface. Surprisingly, their emission spectrum coincides exactly with the plasmon resonance of Ag nanoclusters, located on the surface of the DP NCs.

Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers.

Integrating micro- and nanolasers into live cells, tissue cultures and small animals is an emerging and rapidly evolving technique that offers noninvasive interrogation and labeling with unprecedented information density. The bright and distinct spectra of such lasers make this approach particularly attractive for high-throughput applications requiring single-cell specificity, such as multiplexed cell tracking and intracellular biosensing. The implementation of these applications requires high-resolution, high-speed spectral readout and advanced analysis routines, which leads to unique technical challenges. Here, we present a modular approach consisting of two separate procedures. The first procedure instructs users on how to efficiently integrate different types of lasers into living cells, and the second procedure presents a workflow for obtaining intracellular lasing spectra with high spectral resolution and up to 125-kHz readout rate and starts from the construction of a custom hyperspectral confocal microscope. We provide guidance on running hyperspectral imaging routines for various experimental designs and recommend specific workflows for processing the resulting large data sets along with an open-source Python library of functions covering the analysis pipeline. We illustrate three applications including the rapid, large-volume mapping of absolute refractive index by using polystyrene microbead lasers, the intracellular sensing of cardiac contractility with polystyrene microbead lasers and long-term cell tracking by using semiconductor nanodisk lasers. Our sample preparation and imaging procedures require 2 days, and setting up the hyperspectral confocal microscope for microlaser characterization requires <2 weeks to complete for users with limited experience in optical and software engineering.

Extraction of ocean wave parameters from video images

ABSTRACT In this study, we proposed a method for estimating windsea parameters from the images captured by the surveillance camera installed in coastal areas for practical use. Camera images were acquired in the observation room of the Tokyo Bay Marine Traffic Center at Cape Kannon in Tokyo Bay, Japan for approximately 4 months. Video images of the sea surface were analyzed using in-situ wave observations and wave model data. The brightness spectrum in the frequency domain and the brightness period were defined and introduced from the time series of brightness values in the obtained video images. The novelty of this study lies in the method proposed for estimating the wave parameters using the brightness period. Although this is a simple method, we confirmed that the wave parameters for windsea, such as the significant wave height and significant wave period, can be estimated with high accuracy using the brightness period. Furthermore, we confirmed that the brightness spectrum obtained from camera images and the slope spectrum of wind waves exhibited extremely similar shapes.

PATHOPHYSIOLOGICAL ASPECTS OF HABILITATION AND REHABILITATION OF CHILDREN FROM CHILDREN’S HOMES WITH NEURODEVELOPMENTAL DELAY OF PSYCHOSOCIAL GENESIS

Background. Psycho-emotional stress in institutionalized children leads to significant tension in regulatory systems. Justification for using a complex of habilitation and rehabilitation measures aimed at reducing the stress factor and cre-ating conditions for “catch-up” development is an urgent problem. The aim of the study. To create and to test the effectiveness of a complex of habilitation and rehabilitation measures aimed at reducing deprivation pressure and environmental stress. Material and methods. The study included 114 children from children’s homes of Donetsk and Makeevka and 44 chil-dren from families aged 3 to 7 years. The complex of habilitation and rehabilitation measures included optimization of the daily routine, conditioning exercises, music, toy and fairy tale therapy, sessions of individual play and play in small groups of 2–3 people in a separate playroom, phototherapy with bright solar spectrum lamps, enriched sensory envi-ronment, increased physical activity, differentiated drug support with L-carnitine, glycine, hopantenic acid, magnesium and pyridoxine complex. Neuropsychic development was studied using the Denver Screening Test and the Formalized Card for the Study of Psychoneurological Functions according to I.A. Skvortsov and N.A. Ermolenko. The functions of regulatory systems were assessed by the level of cortisol and melatonin in saliva, and brain-derived neurotrophic factor in blood serum. Conclusion. A comparison of the results of assessing the parameters of neuropsychic development confirmed the effec-tiveness of the proposed complex of habilitation and rehabilitation measures. Restoration of the function of regulatory systems was confirmed by normalization of the rhythms of cortisol and melatonin synthesis, as well as by a significant (3-fold) increase in the content of brain-derived neurotrophic factor in the blood serum in children of the main group.

Chromophores' Contribution to Color Changes of Thermally Modified Tropical Wood Species.

This work examines the effect of thermal modification temperature (180, 200, and 220 °C) in comparison with reference (untreated) samples on selected optical properties of six tropical wood species-Sp. cedar (Cedrala odorata), iroko (Chlorophora excelsa), merbau (Intsia spp.), meranti (Shorea spp.), padouk (Pterocarpus soyauxii), and teak (Tectona grandis). The main goal is to expand the existing knowledge in the field of wood thermal modification by understanding the related degradation mechanisms associated with the formation of chromophoric structures and, above all, to focus on the change in the content of extractive substances. For solid wood, the CIELAB color space parameters (L*, a*, b*, and ΔE*), yellowness (Y), ISO brightness, and UV-Vis diffuse reflectance spectra were obtained. Subsequently, these wood samples were extracted into three individual solvents (acetone, ethanol, and ethanol-toluene). The yields of the extracted compounds, their absorption spectra, and again L*, a*, b*, ΔE*, and Yi parameters were determined. With increasing temperatures, the samples lose brightness and darken, while their total color difference grows (except merbau). The highest yield of extractives (mainly phenolic compounds, glycosides, and dyes) from thermally modified samples was usually obtained using ethanol. New types of extractives (e.g., 2-furaldehyde, lactones, formic acid, some monomer derivatives of phenols, etc.) are already created around a temperature of 180 °C and may undergo condensation reactions at higher temperatures. For padouk, merbau, teak, and partially iroko modified at temperatures of 200 and 220 °C, there was a detected similarity in the intensities of their UV-Vis DR spectra at the wavelength regions corresponding to phenolic aldehydes, unsaturated ketones, quinones, stilbenes, and other conjugated carbonyl structures. Overall, a statistical assessment using PCA sorted the samples into five clusters. Cluster 3 consists of almost all samples modified at 200 and 220 °C, and in the other four, the reference and thermally modified samples at 180 °C were distributed. The yellowness of wood (Y) has a very high dependence (r = 0.972) on its brightness (L*) and the yellowness index of the extractives in acetone Yi(Ac), whose relationship was described by the equation Y = -0.0951 × Y(Ac) + 23.3485.

An inner warp discovered in the disk around HD 110058 using VLT/SPHERE and HST/STIS

Context. An edge-on debris disk was detected in 2015 around the young, nearby A0V star HD 110058. The disk showed features resembling those seen in the disk of β Pictoris that could indicate the presence of a perturbing planetary-mass companion in the system. Aims. We investigated new and archival scattered light images of the disk in order to characterise its morphology and spectrum. In particular, we analysed the disk’s warp to constrain the properties of possible planetary perturbers. Methods. Using data from two VLT/SPHERE observations taken with the Integral Field Spectrograph (IFS) and near InfraRed Dual-band Imager and Spectrograph (IRDIS), we obtained high-contrast images of the edge-on disk. Additionally, we used archival data from HST/STIS with a poorer inner-working angle but a higher sensitivity to detect the outer parts of the disk. We measured the morphology of the disk by analysing vertical profiles along the length of the disk to extract the centroid spine position and vertical height. We extracted the surface brightness and reflectance spectrum of the disk. Results. We detect the disk between 20 au (with SPHERE) and 150 au (with STIS), at a position angle of 159.6° ± 0.6°. Analysis of the spine shows an asymmetry between the two sides of the disk, with a 3.4° ± 0.9° warp between ~20au and 60 au. The disk is marginally vertically resolved in scattered light, with a vertical aspect ratio of 9.3 ± 0.7% at 45 au. The extracted reflectance spectrum is featureless, flat between 0.95 µm and 1.1 µm, and red from 1.1 µm to 1.65 µm. The outer parts of the disk are also asymmetric with a tilt between the two sides compatible with a disk made of forward-scattering particles and seen not perfectly edge-on, suggesting an inclination of &lt;84°. Conclusions. The presence of an undetected planetary-mass companion on an inclined orbit with respect to the disk could explain the warp. The misalignment of the inner parts of the disk with respect to the outer disk suggests a warp that has not yet propagated to the outer parts of the disk, favouring the scenario of an inner perturber as the origin of the warp.

Purely Nitrogen‐Based Multi‐Resonance Deep‐Blue Emitter with an Ultralow y Color Coordinate of &lt; 0.03 via Rationally Intramolecular Charge Transfer

AbstractFor purely nitrogen‐based multi‐resonance emitters (N‐MR), strategies to integrate emission modulation with high color purity remain exclusive, especially in near‐ultraviolet (NUV) regions. Herein, it is demonstrated for the first time that rationally introducing weak intramolecular charge transfer (ICT) to the MR skeleton to modulate the emission from violet to deep‐blue while retaining high color purity is feasible. By replacing the middle phenyl moiety with the pyridine or benzonitrile unit in the solely nitrogen‐based violet MR skeleton of tDIDCz, two proof‐of‐concept emitters, Nm‐ICz and CNm‐ICz, emphasizing mixed excited states of localized excited (LE) transition from the original MR skeleton and emergent transition channel with charge‐transfer (CT) character, successfully realize bathochromic‐shift and polarity‐insensitive fluorescence from 395 nm to 404–407 and 419–421 nm, respectively, while retaining small full‐width at half‐maximums (FWHMs) of 28–37 and 33–43 nm (214‐281 and 237–310 meV). Furthermore, Nm‐ICz shows bright and violet electroluminescence (EL) spectrum with the peak at 412 nm, while CNm‐ICz shows high color purity deep‐blue EL spectrum with the peak at 427 nm, a small FWHM of 42 nm (286 meV), an ultralow y color coordinate of 0.027, and a maximum external quantum efficiency (EQEmax) of 3.7%.

A Technique to Measure Coronal Electron Density, Temperature, and Velocity Above $2.5\ \mathrm{R}_{\odot}$ from Sun Center Using Polarized Brightness Spectrum

A Technique to Measure Coronal Electron Density, Temperature, and Velocity Above $2.5\ \mathrm{R}_{\odot}$ from Sun Center Using Polarized Brightness Spectrum

Study of the electric arc dynamics in a cascaded-anode plasma torch

In plasma spraying process, the plasma jet stability depends on the electric arc movement inside the plasma torch. For conventional plasma torches with rod cathode and a large anodic surface, arc roots are free to move along the anode wall, leading to high plasma jet fluctuations. To overcome these instabilities, cascaded-anode plasma torches have been developed, reducing considerably the electric arc movement and thus the plasma jet fluctuations. Unfortunately, a small number of studies reports on the behaviour of theses torches including the arc instabilities and on electrode erosion process as well. In this work, several diagnostics have been set up to further understand the cascaded-anode plasma torch behaviour under different sets of operating conditions. Influence of plasma forming gas composition on the electric arc dynamic has been studied through anodic imaging. The issue of the intense plasma radiation masking is discussed since the light emitted by the arc column interferes with anodic arc roots observations. Multiple constricted arc roots have been observed and a preferential site for anodic arc root has been identified for all experimental conditions. The addition of a secondary plasma forming gas promotes a higher number of reattachments. The influence of the electric arc movement on plasma jet stability is also studied by following the plasma jet brightness fluctuations. The low voltage fluctuation frequency is also observed on brightness spectra, which means that these fluctuations could have an impact on in-flight particle treatment.

Serendipitous discovery of the magnetic cataclysmic variable SRGE J075818−612027

We report the discovery of SRGE J075818−612027, a deep stream-eclipsing magnetic cataclysmic variable found serendipitously in SRG/eROSITA calibration and performance verification phase (CalPV) observations of the open cluster NGC 2516 as an unrelated X-ray source. An X-ray timing and spectral analysis of the eROSITA data is presented and supplemented by an analysis of TESS photometry and SALT spectroscopy. X-ray photometry reveals two pronounced dips repeating with a period of 106.144(1) min. The 14-month TESS data reveal the same unique period. A low-resolution identification spectrum obtained with SALT displays hydrogen Balmer emission lines on a fairly blue continuum. The spectrum and the stability of the photometric signal led to the classification of the new object as a polar-type cataclysmic variable. In this context, the dips in the X-ray light curve are explained by absorption in the intervening accretion stream and by a self-eclipse of the main accretion region. The object displays large magnitude differences on long timescales (months) both at optical and X-ray wavelengths, which are interpreted as high and low states and thus support its identification as a polar. The bright phase X-ray spectrum can be reflected with single temperature thermal emission with 9.7 keV and bolometric X-ray luminosity LX ≃ 8 × 1032 erg s−1 at a distance of about 2.7 kpc. The X-ray spectrum lacks the pronounced soft X-ray emission component prominently found in ROSAT-discovered polars.

Temporal albedo variability in the phase curve of KELT-1b

The dayside brightness spectrum of a highly irradiated transiting brown dwarf KELT-1b is challenging to explain based on current brown dwarf atmosphere models. The spectrum has been measured from observations spanning ten years and covering high-precision secondary eclipses and phase curves from space in blue-visible (CHaracterising ExOPlanet Satellite, CHEOPS), red-visible (Transiting Exoplanet Survey Satellite, TESS), and near-infrared (Spitzer), as well as secondary eclipse observations in near-infrared from the ground. First, the dayside of KELT-1b was observed to be brighter in the TESS passband than expected, based on earlier near-infrared phase curve observations with Spitzer, and, recently, the dayside was observed to be extremely dark in the CHEOPS passband. While several theories have been proposed to reconcile the discrepancy between the TESS and Spitzer bands, explaining the difference between the largely overlapping CHEOPS and TESS bands has proven more difficult. In this work, I model the TESS photometry from Sector 17 together with the new TESS photometry from Sector 57 and show that the discrepancies in KELT-1b’s dayside brightness spectrum are best explained by temporal variability in KELT-1b’s albedo. This variability is most likely due to changes in the weather, namely, variations in the silicate cloud coverage on the brown dwarf’s dayside.

Fluorescent HPHT nanodiamonds have disk- and rod-like shapes

Fluorescent nanodiamonds (FNDs) containing nitrogen-vacancy (NV) centers can be used as nanoscale sensors for temperature and electromagnetic fields and find increasing application in many areas of science and technology from biology to quantum metrology. Decreasing the separation between the NV centers and their sensing target often enhances the measurement sensitivity. FND shape strongly affects this distance from NV centers to the particle surface and therefore properties such as brightness and fluorescence spectrum, and can limit sensor applications. Here, we demonstrate that FNDs made from high-pressure high-temperature (HPHT) diamond have predominantly disk-like shapes. Using single-particle atomic force microscopy in combination with ensemble X-ray and light scattering techniques, we show that a typical FND in the 50–150 nm size range has an aspect ratio of three i.e. is three times thinner (e.g. in z) than it is wide (e.g. in the x-y plane). This high aspect ratio of FNDs is important for many quantum sensing measurements as it will enable enhanced sensitivities compared to spherical or other isotropic particle geometries. We investigate FND shape, fluorescence properties, T1 spin relaxation time and T1 fluorescence contrast as functions of particle size and discuss the implications of FND particle shape on quantum sensing applications.

Expanding the color palette of bicolor-emitting nanocrystals.

Owing to their bright and tunable luminescence spectra, nanocrystals appear as a unique playground for light source design. Displays and lighting require white light sources that combine several narrow lines. As Kasha's rule prevents the emission of hot carriers, blends of multiple nanocrystal populations are currently the obvious strategy for broad-band source design. However, a few reports suggest that bicolor emission can also be obtained from a single particle even under weak excitation if a careful design of the exciton scattering mechanism sufficiently slows down its relaxation pathways. A key challenge remains to maintain quantum confinement for color tunability in the same structure, while simultaneously achieving a large size to leverage the critical, slower exciton diffusion or relaxation down to the ground state. Herein, we demonstrate that 2D nanoplatelets offer an original opportunity for the design of confined and large heterostructures. We demonstrate that bicolor emission is not limited to green-red pair and show that blue-yellow and purple-green emissions can be obtained from CdSe/CdTe/CdSe core/crown/crown and CdSe/CdS core/crown heterostructures, respectively.

Choosing the Probe for Single-Molecule Fluorescence Microscopy

Probe choice in single-molecule microscopy requires deeper evaluations than those adopted for less sensitive fluorescence microscopy studies. Indeed, fluorophore characteristics can alter or hide subtle phenomena observable at the single-molecule level, wasting the potential of the sophisticated instrumentation and algorithms developed for advanced single-molecule applications. There are different reasons for this, linked, e.g., to fluorophore aspecific interactions, brightness, photostability, blinking, and emission and excitation spectra. In particular, these spectra and the excitation source are interdependent, and the latter affects the autofluorescence of sample substrate, medium, and/or biological specimen. Here, we review these and other critical points for fluorophore selection in single-molecule microscopy. We also describe the possible kinds of fluorophores and the microscopy techniques based on single-molecule fluorescence. We explain the importance and impact of the various issues in fluorophore choice, and discuss how this can become more effective and decisive for increasingly demanding experiments in single- and multiple-color applications.

Multipolar Raman Scattering vs Interfacial Nanochemistry: Case of 4-Mercaptopyridine on Gold.

Caution needs to be exercised in associating changes in plasmon-enhanced Raman spectra with chemical transformations. This is demonstrated through a detailed analysis of tip-enhanced Raman (TER) scattering from 4-mercaptopyridine (MPY) on gold. The substrate used consists of gold nanoplates atop a gold surface featuring heterogeneous grooves, all coated with a monolayer of MPY. The brightest spectra across the substrate exhibit features that can only be recovered by considering the generalized polarizability of oriented MPY molecules. The complex TER spectra we observe do not mark interfacial chemistry but rather multipolar TER scattering driven by local field gradients.

Phosphoric acid assisted synthesis of fluorescent carbon dots from waste biomass for detection of Cr(VI) in aqueous media

A green and facile method for the synthesis of carbon dots (CDs) have been developed using lignocellulosic biomass (banana stem) and phosphoric acid to construct fluorescent sensor for Cr(VI) detection. The effects of phosphoric acid and temperature on the fluorescence behavior of carbon dots have been explored elaborately. The optical and structural properties of the CDs were determined by different characterization techniques. The prepared CDs display excellent fluorescence properties with intense bright blue emission spectra around 447 nm at an excitation wavelength of 350 nm. The CDs possessing the highest quantum yield (15.1%) have an immense sensing potential to detect Cr(VI) in linear detection range (10–30 μM) with detection limit of 2.4 μM. Furthermore, the CDs have been applied to quantitatively detect Cr(VI) in real water samples. Theoretical studies based on DFT and TDDFT calculations suggested the detection pathway of Cr(VI) occurs via photoinduced electron transfer (PET) which furthur supports the experimental studies.