The generalized uncertainty relation is introduced to calculate entropy of the black hole. By using quantum statistical method, we directly obtain the partition function of Bose and Fermi field on the background of the plane symmetry black hole. Then we calculate the entropy of Bose and Fermi field on the background of black hole near the horizon of the black hole. In our calculation, we need not introduce cutoff. There are not the left out term and the divergent logarithmic term in the original brick-wall method. And it is obtained that the entropy of the black hole is proportional to the area of the horizon. The inherent contact between the entropy of black hole and the area of horizon is opened out. Further it is shown the entropy of black hole is entropy of quantum state on the surface of horizon. The black hole’s entropy is the intrinsic property of the black hole. The entropy is a quantum effect.