Brewery Waste Research Articles

Adsorption of low molecular weight food relevant polyphenols on cross-linked agarose gel

Adsorption of five relevant low molecular weight polyphenols identified in agro-industrial waste extracts (Aristotelia chilensis leaves, Carménère wine pomace, spent coffee grounds, and brewery waste streams) was measured and characterized. SuperoseTM 12 prep grade and between three and six solutions with different water, ethanol, and acetic acid compositions were used as adsorbent and liquid phases. The chosen adsorbent and liquid phases were relevant for designing an adsorption preparative liquid chromatography (APLC) process to isolate these polyphenols. Langmuir and Freundlich models adequately fitted the obtained isothermal equilibrium data. The Freundlich model represented better ferulic acid (FA), kaempferol (KAE), and resveratrol (RSV) adsorptions, while the Langmuir model represented better gallic acid (GA) and catechin (CAT). Different polyphenol/agarose affinities in water-rich liquid phases were observed. From this, a hypothetical elution order was established (KAE < GA < FA < CAT < RSV), which was partially experimentally corroborated (for a mixture of GA, CAT, and RSV) with an APLC system. Lowering the water proportion or increasing the EtOH:HAc ratio in the liquid phase reduced the adsorption of these polyphenols, except for FA. The decrease in adsorption with temperature and the negative values of ΔH indicated that these processes were exothermic. The adsorption of all the polyphenols was governed by physisorption. All the adsorption processes studied were spontaneous and thermodynamically feasible (ΔG < 0). In addition, the polyphenol molecules were less randomly organized (more ordered) at the polyphenol/agarose interface during the adsorption process (ΔS < 0).

In Situ Bioremediation of a Chlorinated Hydrocarbon Plume: A Superfund Site Field Pilot Test

The North Railroad Avenue Plume, discovered in 1989, contained chlorinated solvent groundwater plumes extending over 23.5 hectares (58 acres) and three hydrostratigraphic units. The source contaminant, tetrachloroethene, stemmed from release at a dry cleaner/laundromat business. The anaerobic biodegradation byproducts trichloroethene, isomers of dichloroethene (DCE), and vinyl chloride were detected in groundwater samples collected prior to remedial action. The impacted aquifers are the sole source drinking water aquifers for the communities near the site. Following the remedial investigation and feasibility study, the selected alternative for full-scale remedial action at the site was enhanced reductive dichlorination (ERD) focused on four treatment areas: the shallow source zone, the shallow hotspot area, the shallow downgradient area, and the deep zone. Pilot testing, which was conducted in the source zone and hotspot areas, is the subject of this paper. The primary objectives of the pilot test were to obtain the necessary information to select an ERD treatment formulation, dose, and frequency of dosing for use during full-scale remedial action, as well as to refine the site’s hydrogeologic conceptual site model and design parameters. Four (4) test cells, each of which contained well pairs of injection and downgradient extraction wells, were used to test ERD bio-amendment formulations: ethyl lactate, dairy whey, emulsified vegetable oil (EVO), and a combination of EVO and a hydrogen gas infusion. A conservative tracer, bromide, was added to the recirculation flow to record tracer breakthrough, peak, and dissipation at extraction wells. The results of these dipole tracer tests were used to reassess the hydraulic conductivity and hydrodynamic dispersity used in the remedial design. In addition to water quality analyses of contaminants and substrates, groundwater samples were also analyzed for biological analyses before, during, and after the addition of bioamendment. Analyses of phospholipid fatty acids and deoxyribonucleic acid (DNA) extracts from fresh groundwater samples informed decisions on the capacity for complete ERD without DCE stalling and tracked the shifts in the bacterial and archaeal taxonomy and phylogeny stemming from the addition of bioamendments. The pilot test concluded that EVO was the most suitable, considering (1) support of the native microbial consortia for ERD, (2) mechanics and hydraulics of the remediation system, and (3) sustainability/retention of the substrate in the subsurface. Along with EVO, the addition of a nutrient broth derived from brewery waste accelerated and sustained the desired conditions and microbial diversity and population levels. The pilot test results were also used to assess the utilization kinetics of the injected substrates based on total organic carbon (TOC) concentrations measured in the groundwater. After determining that substrate utilization followed Monod kinetics, a TOC threshold at 300 milligrams per liter, equivalent to approximately twice its half-saturation constant was established. Full scale treatment dosing and dose frequency were designed around this threshold, assuming the maximum substrate utilization would yield optimum ERD.

Soybean hulls inclusion on silage of wet brewery waste

This study aimed to evaluate the effects of soybean hulls inclusion in the silage of wet brewery waste. The experimental design was randomized in block, where the treatments were constituted by four levels of soybean hulls on the silage (0, 15, 25, and 35%), with three (silos) replicates per treatment. All data collected were subjected to ANOVA and subsequent polynomial regression at 5%. Soybean hulls inclusion caused a linear increase (p &lt; 0.05) in the pH, dry matter, acid detergent fiber, neutral detergent fiber and total carbohydrates content of the silage. This inclusion also caused a significant reduction (p &lt; 0.05) in the effluent losses and percentages of crude protein, fats, hemicellulose, non-fibrous carbohydrates and total digestible nutrients. Thus, it was concluded that soybean hulls can be used as an additive in the silage of wet brewery waste. Up to 35% of inclusion, there was a significant reduction in the effluent losses, a little increase on pH and enrichment of nutritional content, especially in the dry matter.

Valorization of Brewery Wastes for the Synthesis of Silver Nanocomposites Containing Orthophosphate.

Brewery wastes from stage 5 (Wort precipitate: BW5) and stage 7 (Brewer’s spent yeast: BW7) were valorized for the synthesis of silver phosphate nanocomposites. Nanoparticles were synthesized by converting silver salt in the presence of brewery wastes at different temperatures (25, 50, and 80 °C) and times (10, 30, and 120 min). Unexpectedly, BW7 yielded Ag3PO4 nanoparticles with minor contents of AgCl and Ag metal (Agmet). Contrastingly, BW5 produced AgCl nanoparticles with minor amounts of Ag3PO4 and Agmet. Nanocomposites with different component ratios were obtained by simply varying the synthesis temperature and time. The morphology of the nanocomposites contained ball-like structures representative of Ag3PO4 and stacked layers and fused particles representing AgCl and Agmet. The capping on the nanoparticles contained organic groups from the brewery by-products, and the surface overlayer had a rich chemical composition. The organic overlayers on BW7 nanocomposites were thinner than those on BW5 nanocomposites. Notably, the nanocomposites exhibited high antibacterial activity against Escherichia coli ATCC 25922. The antibacterial activity was higher for BW7 nanocomposites due to a larger silver phosphate content in the composition and a thin organic overlayer. The growth of Agmet in the structure adversely affected the antimicrobial property of the nanocomposites.

Antibiotic Resistance of Escherichia coli Isolated from Processing of Brewery Waste with the Addition of Bulking Agents

The aim of the study was to determine the drug resistance profile and to assess the presence of genes responsible for the production of extended-spectrum beta-lactamases in Escherichia coli isolated from energy-processed hop sediment with the addition of bulking agents. Antibiotic resistance was determined by the disk diffusion method and the PCR technique to detect genes determining the extended-spectrum beta-lactamases (ESBLs) mechanism. A total of 100 strains of E. coli were collected. The highest resistance was found to aztreonam, tetracycline, ampicillin, ticarcillin, and ceftazidime. The bacteria collected were most often resistant to even 10 antibiotics at the same time and 15 MDR strains were found. The ESBL mechanism was determined in 14 isolates. Among the studied genes responsible for beta-lactamase production, blaTEM was the most common (64%). The study revealed that the analysed material was colonised by multi-drug-resistant strains of E. coli, which pose a threat to public health. The obtained results encourage further studies to monitor the spread of drug resistance in E. coli.

A low-cost brewery waste as a carbon source in bio-surfactant production.

This work aims to produce bio-surfactant using a brewery waste (trub) as a strategy to reduce production costs related to the substrate, as well as to provide an eco-friendly destination for this residue. Trub is obtained during the boiling of the wort, being mainly composed of proteins and reducing sugars. To evaluate important process parameters on bio-surfactant production, a full factorial design (24) was elaborated, having agitation rate and concentrations of trub, yeast extract, and peptone as independent variables. The highest bio-surfactant concentration achieved was 100.76mgL-1, where FTIR and Maldi-ToF-MS confirmed functional groups characteristic of peptides and isomers of surfactin in the bio-surfactant extract. Trub, agitation and yeast extract showed statistically significant effects on the response variable (surface tension), where an increase in the agitation rate and in the concentration of yeast extract demonstrated a positive impact on the production of bio-surfactant.

Anaerobic Digestion of Food Waste, Brewery Waste, and Agricultural Residues in an Off-Grid Continuous Reactor

Small-scale anaerobic digestion (AD) can be an effective organic waste management system that also provides energy for small businesses and rural communities. This study measured fuel production from digestions of single and mixed feedstocks using an unheated, 2 m3 digester operated continuously in a temperate climate for over three years. Using local food waste, brewery waste, grease waste, and agricultural residues, this study determined that small-scale AD co-digestions were almost always higher yielding than single feedstocks during psychrophilic operation and seasonal temperature transitions. Agricultural residues from Miscanthus x giganteus had the greatest impact on biomethane production during co-digestion (4.7-fold greater average biogas %CH4), while mesophilic digestion of brewery waste alone produced the most biogas (0.76 gCH4 gVS−1 d−1). Biogas production during the transition from mesophilic to psychrophilic was temporarily maintained at levels similar to mesophilic digestions, particularly during co-digestions, but biogas quality declined during these temperature shifts. Full-time operation of small-scale, unheated AD systems could be feasible in temperate climates if feedstock is intentionally amended to stabilize carbon content.

Bioconversion of low-cost brewery waste to biosurfactant: An improvement of surfactin production by culture medium optimization

Trub is the second-largest residue from brewing industry (250 thousand tons/year) and, due to the presence of high amounts of carbon and nitrogen, it is a potential source of reducing sugars and nitrogen for biological processes. Therefore, this work aimed to enhance surfactin production by Bacillus subtilis using Trub as a carbon source. The effect of nitrogen sources on biosurfactant production was investigated by a full factorial design (22). Yeast extract and its interaction with peptone presented a statistically significant effect on the response variable of surface tension (ST). ST was described by a linear regression model (R2 = 0.93). The effect of metals supplementation on the culture medium was also investigated, where the volumetric productivity increased from 2.98 to 7.43 mg L−1 h−1 for the medium without (M-I) and with metals (M-II), respectively. After 28 h, 210.11 mg L−1 was obtained in the batch culture with M-II medium, being higher than the maximum surfactin concentration achieved after 52 h (121.80 mg L−1) for M-I medium. The biosurfactant production in bioreactor was successfully performed since a concentrated extract was obtained in the foam collected, reaching the maximum of 1118.20 mg L−1 of surfactin.

Kinetic modeling of biosurfactant production by Bacillus subtilis N3-1P using brewery waste

Abstract Costs associated with production of favorable biologically produced surfactants continue to be a significant obstacle to large scale application. Using industrial wastes and by-products as substrate and optimization of cultural conditions are two strategies of producing biosurfactants with a reasonable price. Also, modeling the biosurfactant production bioprocess improves the commercial design and monitoring of biomass growth, biosurfactant production, and substrate utilization. In this study, the indigenous Bacillus subtilis N3-1P strain and a local brewery waste as the carbon source were used to produce a biosurfactant. The batch cultivation was performed under the optimum conditions. Models describing the biomass growth, biosurfactant production, and substrate utilization were developed by fitting the experimental data to the logistic, Contois and Luedeking-Piret models using MATLAB software and regression analysis. The kinetic parameters including the maximum specific growth rates (µ max), the Contois constant (K), parameters of the Luedeking-Piret modelswere calculated. Yields including Y X/S , and Y P/X were found to be 0.143 gX/gS, and 0.188 gP/gX, respectively. The experimental and predicted model showed good agreement. The developed models are a key step in designing reactors for scale up of biosurfactant production.

Characteristics of carcass and non-carcass components of lambs fed wet brewery waste as a roughage feed

The objective of the present study was to evaluate the effect of including wet brewery waste (WBW) as the exclusive dietary roughage source on the characteristics of carcass and non-carcass components of feedlot-finished lambs. Thirty-two non-castrated male lambs weaned at 50 days of age, resulting from the cross between the Texel and Ile de France breeds, were used. The diet was constituted by roughage (WBW) and a concentrate composed of crushed maize, soybean meal, limestone and common salt. Treatments consisted of four levels of WBW as the roughage source in the diet (% dry matter), namely, 31, 44, 57 and 70%. Lambs were slaughtered upon reaching 34 kg live weight. The treatments resulted in a linear decrease in hot and cold carcass weights and yields, carcass compactness index, conformation, degree of fatness and rib-eye area. Among the primal cuts, the absolute weights of pallet, ribs and legs decreased as the WBW level was increased. As to the non-carcass components, only the total gastrointestinal content increased linearly with WBW. The increasing levels of WBW led to greater fasting losses and reduced carcass weights and yields. There was also a reduction in the degree of fatness, carcass compactness, carcass conformation index and rib-eye area Wet brewery waste at the concentration of 31% as the roughage source in the diet of finishing lambs in the feedlot provides better carcass traits.

Comparative assessment of developmental parameters, proximate analysis and mineral compositions of black soldier fly (Hermetia illucens) prepupae reared on organic waste substrates

The increasing demand for alternative feed sources for livestock has evolved, owing to quest for efficient and sustainable use of resource channeled towards achieving food security. Breeding and biomass conversion of selected insects therefore, is imperative to complement existing sources. This study investigated the developmental parameters, and nutritional composition of Black soldier fly (BSF) (Hermetia illucens) larvae reared on chicken feed (CF), brewery waste (BW), food remains (FR) and fruits’ waste (FR). Proximate compositions (%) of prepupae and substrates were analyzed for dry matter (DM), crude protein (CP), ether extracts (EE), ash, fibre and mineral composition. Data were analyzed using analysis of variance (ANOVA) tests and Tukey (homoscedasticity) using SPSS statistical package. CF recorded the highest prepupal weight (0.30 g), length (2.18 cm), and shortest development time (21 days) across the different substrates. CP content was highest in prepupa fed on CF and BW at 48.93% and 44.52% respectively (p < 0.05). The ash contents and EE of the prepupae differed significantly across the different substrates. Prepupae reared on CF were low in EE and high in ash content (4.12% and 9.24%, respectively) compared to those reared on FW (13.90% and 5.24%, respectively), BW (11.23% and 10.29%, respectively) and FR (9.52% and 5.24, respectively). BSF larvae fed on different substrates exhibited different mineral accumulation patterns, with FW resulting in the largest turnover of minerals (Potassium 46.94 g/kg). The rearing of BSF larvae can be optimized on identified organic substrates into edible biomass suitable for use as an alternative protein source.

Разработка инновационного способа получения биологически активных соединений пивной дробины

Introduction. Brewery mash, or brewer’s spent grain (BSG), is a by-product of brewing industry. It is known to contain valuable biologically active substances. However, their extraction is complicated by the presence of various polymers. The research featured various physicochemical methods for obtaining valuable biological compounds from brewery waste. The new method modified complex non-starch polysaccharides, lignin, arabinoxylans, and other high-molecular compounds associated with phenolic compounds. The research objective was to solve the problem of recycling industrial by-products that accumulate in large quantities and require expensive processing or disposal. The paper introduces new technological approaches for deep processing of BSG as a source of secondary raw materials in order to obtain extracts fortified with polyphenolic compounds.&#x0D; Study objects and methods. The research featured BSG from malt subjected to treatment with ECA-activated water (catholyte with pH 9.6 ± 0.1), followed by enzymatic hydrolysis of cellulolytic enzyme preparations and extraction with a polar solvent of the resulting free polyphenolic substances. The experiments were based on standard methods for assessing the content of various biologically active substances.&#x0D; Results and discussion. A 70% water-ethanol solution proved to be optimal at the BSG:extractant ratio of 2:1, process temperature = 50 ± 2°C, and extraction time = 60 ± 5 min. Under the same conditions, 70 %vol. of beer distillate made it possible to extract phenolic acids, flavonoid rutin, irreplaceable and nonessential amino acids, and non-starch polysaccharide β-glucan from the BSG matrix. The BSG treatment with 1M NaOH solution delivered viscous hydrolysates fortified with flavonoids rutin and quercetin, which did not happen when acid hydrolysis was used. The combined use of ECA-treated water (catholyte with pH 9.6 ± 0.1) for 24 ± 0.05 h, combined with biocatalysis with the enzyme preparation Viskoflo MG for 2 ± 0.05 h, made it possible to obtain BSG extracts with a high content of phenolic acids and aldehydes, as well as flavonoid rutin.&#x0D; Conclusion. The study revealed the mechanism of hydrolytic decomposition of BSG non-starch polysaccharides, considering the compounds contained in the extracts. The BSG hydrolysates fortified with various phenolic compounds can be used in various food technologies, e.g., in fermented drinks.

Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

This work reports surfactin production by B. subtilis ATCC 6051 in a stirred tank bioreactor, where trub was used as a carbon source. Trub is a residue from the brewing industry mainly composed of high-molar-mass proteins and reducing sugars, being a sustainable and low-cost substrate for bioprocess. Despite this, Trub has been little explored as a raw material in bioprocesses. Aiming to evaluate important factors in scale-up process, the impact of oxygen transfer coefficient (KLa) concerning volumetric productivity, and the effect of agitation (150–300 rpm) and aeration (0.5–1.5 vvm) on biosurfactant production by a Full Factorial design were evaluated. Only agitation presented a significant effect on surfactin concentration (SF), where SF was described by a linear regression model (R2 = 0.99). The biosurfactant showed high stability in relation to variations in temperature and pH. In addition, the foam collecting was an efficient method in recovering and enrichment of the product.

Treatment of brewery industrial wastewater and generation of sustainable bioelectricity by microbial fuel cell inoculated with locally isolated microorganisms

The chemical energy contained in wastewater can be considered as a promising sustainable energy source and can be recovered using microbial fuel cell technology by means of electro-active bacteria. There are several brewery industries releasing wastewater into the environment, posing serious environmental issues. Herein, we demonstrated treatment of brewery wastewater and production of bioelectricity simultaneously using double chamber MFCs by inoculating locally isolated microorganisms. Microorganisms were locally isolated from brewery waste sludge (named as BSGB1 and BSGB2), brewery wastewater (BWGB3 and BWGB4) and food processing industry waste sludge (FSGB5 and FSGB6). Total dissolved solids (TDS), chemical oxygen demand (COD), and biochemical oxygen demand (BOD) were determined before and after treatment of brewery wastewater by locally isolated microorganisms. The results revealed that the microorganisms isolated from brewery waste sludge outperformed the bacteria isolated from brewery wastewater and food processing industry waste sludge. The maximum power density of 0.8 W/m3 and 0.35 W/m3 was generated by MFC inoculated with locally isolated microorganisms from brewery waste sludge using the synthetic and real-brewery wastewater, respectively. The removal efficiency of COD was 79−83%, indicating significant treatment of brewery wastewater by locally isolated microorganisms while generating sustainable and clean energy.

Heavy metal bioaccumulation in prepupae of black soldier fly Hermetia Illucens (Diptera: Stratiomyidae) cultured with organic wastes and chicken feed

Larvae of the black soldier fly (BSF), Hermetia illucens can be reared in large quantities on organic wastes. The larvae are harvested at the prepupal stage and used as feed for poultry and aquaculture. BSF farms in Nigeria make use of a combination of chicken feed and organic waste in the rearing processes. Chicken feed and organic waste can contain varying levels of heavy metals, which may accumulate in the larvae. The aim of this study was to determine bioaccumulation of heavy metals, cadmium chromium, copper, lead, and zinc, in prepupae cultured on chicken mash and various types of organic wastes (food waste, brewery waste, and fruit waste). Bioaccumulation of a particular heavy metal occurs when the concentration of the metal is higher in the harvested prepupae than in the feed substrates (Factor > 1). The results of the study showed that bioaccumulation was more likely to occur in brewery waste than in the other substrates, while bioaccumulation factors greater than one were reported for three of the five heavy metals (copper, lead and chromium). All heavy metal concentrations reported in the study were below the safe limits for use in animal feeds. The use of organic waste rather than chicken mash for rearing BSF larvae should be considered in Nigeria. However, there must be quality control measures in place to ensure that the larvae’s propensity for accumulating heavy metals are contained.

Dynamic Simulation and Modelling of Methane Production Process for Habesha Beer Waste Water Treatment Process Using Aspen Plus Software

Brewery production process is one of the biggest economical sources in Ethiopia, there are different brands in beer industry in the country, such as Dashen, Balageru, Heineken, and Habesha Brewery Company, in such factories the modern technology treatment was performed in Habesha brewery. All beer industrial processes require large amounts of water and produce significant amounts of wastewater. Cleaner production is having an impact on these industries and today they are looking forward to zero discharge. In Habesha brewery, the generation of wastewater is coming under all departments, such as from brew house, filtration utility, and packaging rooms. The composition of brewery waste water effluent was sulphate, H2S, CO2, sugar, and methane, etc. Habesha Brewery has given value highly the quality, taste, and drinkability of their beer. Production of methane from waste water during the treatment process was not applied in Habesha brewery industry due to the low treatment of methane and lack of technology to recover methane as an energy source. Through anaerobic digestion of organic waste materials provides an alternative environmentally friendly renewable energy. Methane production from digestion of brewery wastewater was improved using chemical methods, using aspen plus dynamics simulation the model of methane production recovery system was modelled, and brewery waste was taken into the reactor in its own behavior was evaluated under atmospheric conditions, 25°C, 30°C & at 1 atmosphere. For all substrates, total solids, biological oxygen demand, chemical oxygen demand, temperature, total nitrogen, total phosphorus, and pH were measured before and after digestion. Wastewater by volume was taken fully into the digester. All measured physico-chemical parameters of the sample substrate significantly varied before and after anaerobic digestion. Methane was measured for all samples periodically starting from the effluent was started. In all substrates. Assessment of cumulative methane production revealed that the substrate at a temperature 30°C, pH 6.5 (0.0013 hl /0.008 hL sample of waste water) and after 4 days sample 25°C, PH 7.5 (0.001625 hL/0.008 hL sample) showed the highest Methane production and the lowest was in the last day volume waste water, 35°C temperature and pH of 6 (0.00125 hL/0.008 hL sample of waste water). The results indicated that the methane amount was affected by H2S, CO2, sugar, and other behaviors of brewery waste water effluent during the digestion process.

DOI: 10.1002/chemv.202100049 Author: ChemistryViews Published Date: 31 Mai 2021 Copyright: Wiley-VCH GmbH thumbnail image: Renewable Energy Is Growing – But Not Fast Enough Related Articles News: Technical Corn Oil for Renewable Diesel News: Investing in Renewable Fuels News: Together Must Be The Motto in Europe Magazine: Brewery Waste as Renewable Energy Source For the first time, renewables have overtaken fossil

DOI: 10.1002/chemv.202100049 Author: ChemistryViews Published Date: 31 Mai 2021 Copyright: Wiley-VCH GmbH thumbnail image: Renewable Energy Is Growing – But Not Fast Enough Related Articles News: Technical Corn Oil for Renewable Diesel News: Investing in Renewable Fuels News: Together Must Be The Motto in Europe Magazine: Brewery Waste as Renewable Energy Source For the first time, renewables have overtaken fossil

Use of Natural Wastes for Biosurfactant (BS) and Bioemulsifier (BE) Production and their Applications – A Review

Commercial biosurfactant (BS) or bioemulsifier (BE) production requires high manufacturing cost and result difficulties in downstream processing and purification. This problem can be resolved by using low- cost natural substrates. Agro- industrial wastes as well as non-edible portions of fruits, vegetables, fish and meat contributes in high disposal and loss of nutritional biomass from the environment. These are readily available wastes which have tremendous potential to be reused as a substrate by microorganisms for efficient BS or BE production. Fruits, vegetables, fish, dairy and brewery wastes are rich sources of valuable nutrients which includes carbon, nitrogen, vitamins and other minerals. BS or BE produced using these substrates are stable in environment and show potential applications in many sectors of food industry, oil industry, agriculture, bioremediation, medicine and pharmaceutical industry. Yield of biosurfactant or bioemulsifier production can be increased by optimizing certain media parameters with the natural substrate concentrations. Growth parameters such as pH, temperature, salinity, carbon and nitrogen content have effect on stability of microorganism for maximum biosurfactant or bioemulsifier production. This review describes some recent developments and applications for the commercial biosurfactant or bioemulsifier production using cheap and unconventional natural wastes.

Adsorption Kinetics of Toxic Metals from Brewery Waste Water Using Adsorbent Prepared from Borassus aethiopum

Adsorption Kinetics of Toxic Metals from Brewery Waste Water Using Adsorbent Prepared from Borassus aethiopum

Development of natural pigments microencapsulated in waste yeast Saccharomyces cerevisiae using spray drying technology and their application in yogurt.

Although Saccharomyces cerevisiae has shown potential utilization as a bio-vehicle for encapsulation, there are no reports about the functionality of natural colorants encapsulated using yeast cells. The main objectives of this study were to produce natural food coloring by encapsulating extracts from grape pomace (GP) and jabuticaba byproducts (JB) in brewery waste yeast and evaluate the functionality of the pigments by their incorporation into yogurts. Particles produced by the encapsulation of extracts from GP and JB in S. cerevisiae using 5% of yeast had the highest encapsulation efficiencies for both anthocyanins (11.1 and 47.3%) and phenolic compounds (67.5 and 63.6%), the highest concentration of both bioactives during storage and stable luminosity. Yogurts showed a pseudoplastic behavior and were considered weak gels. Colored yogurts had acceptance indexes between 73.9 and 81.4%. This work evidenced the utilization of enriched yeasts as coloring agents and interesting additives for the production of functional foods.