Goat Breeds Research Articles

Whole-genome resequencing reveals diversity and selective signals in the Wuxue goat.

Animal genetic resources are crucial for ensuring global food security. However, in recent years, a noticeable decline in the genetic diversity of livestock has occurred worldwide. This decline is pronounced in developing countries, where the management of these resources is insufficient. In the current study, we performed whole genome sequencing for 20 Wuxue (WX) and five Guizhou White (GW) goats. Additionally, we utilized the published genomes of 131 samples representing five different goat breeds from various regions in China. We investigated and compared the genetic diversity and selection signatures of WX goats. Whole genome sequencing analysis of the WX and GW populations yielded 120 425 063 SNPs, which resided primarily in intergenic and intron regions. Population genetic structure revealed that WX exhibited genetic resemblance to GW, Chengdu Brown, and Jintang Black and significant differentiation from the other goat breeds. In addition, three methods (nucleotide diversity, linkage disequilibrium decay, and runs of homozygosity) showed moderate genetic diversity in WX goats. We used nucleotide diversity and composite likelihood ratio methods to identify within-breed signatures of positive selection in WX goats. A total of 369 genes were identified using both detection methods, including genes related to reproduction (GRID2, ZNF276, TCF25, and SPIRE2), growth (HMGA2 and GJA3), and immunity (IRF3 and SRSF3). Overall, this study explored the adaptability of WX goats, shedding light on their genetic richness and potential to thrive in challenges posed by climatic changes and diseases. Further investigations are warranted to harness these insights to enhance more efficient and sustainable goat breeding initiatives.

Review: Goat and Sheep Meat Production in Indonesia

This review describes the production systems and products pertaining to goats and sheep (GS) in Indonesia. These small ruminant species are found across 38 provinces in Indonesia. Almost 99% of the GS are farmed by smallholders, with the animals either being housed in an enclosure (small shed near the farmer’s house) or in a mix of both grazing and confinement. The livestock are typically fed natural and/or artificial feed. Two sheep types, thin-tailed and fat-tailed are the most popular with smallholder producers, while Bali, Etawah, Boerawa, Jawa Randu Kosta, and Gembrong are the dominant goat breeds. As one of the biggest Muslim countries in the world, Indonesian production and consumption of GS is influenced by culture and religious systems. The Halal slaughter method applied is described, as are the religious/cultural festivals in Indonesia, which have a significant relationship on the GS demand and consumption, such as Eid al-Adha and Aqiqah. The preparation, processing, and consumption of GS meat in Indonesia are strongly influenced by the use of spices, being part of the Moluccas archipelago, also known as the “Spice islands” with over 30,000 species of spices grown. Some traditional meats and meat processing technologies in Indonesia are presented to provide insight into how GS meat is processed. The main challenges in the processing of GS meat in Indonesia and some current research on the development of GS production are presented in this paper. Implications: Goat and sheep belong to the most widely consumed meats in the world after pork, poultry, and beef. There are some reasons why GS meat has become one of the more popular protein choices for many families, of which its low-fat levels are one. GS meat is also considered one of the only meats with minimal religious taboos in the world. For these reasons, GS meat is also favored by Indonesian people in the most populous Muslim-majority country. However, information regarding goat and sheep in Indonesia is still lacking. This review aims to provide information on the distribution of GS in Indonesia over 38 provinces, the breed types that grow there, their production, and Indonesian consumption behavior, highlighting the religious festivals that cause the highest demand for these meats. This review provides information to the stakeholders of goat and sheep production chains.

Genetic parameters for reproductive traits and somatic cell score in U.S. dairy goats.

There are no studies regarding the estimation of genetic parameters and genetic trends for reproductive traits and somatic cells in goats. Their knowledge allows optimization of selection schemes. The objective of this study was to estimate genetic parameters and genetic and phenotypic trends for age at first kidding (AFK), kidding interval (KIN) and somatic cell score (SCS). Analyses were conducted within and across seven US goat breeds, namely, Nubian (NU), Alpine (AL), LaMancha (LM), Toggenburg (TO), Saanen (SA), Nigerian Dwarf (ND) and Oberhasli (OB), and a set of all of these breeds (AB). The restricted maximum likelihood methodology and trivariate animal models were used. Genetic and phenotypic trends were estimated using regression models. The average and standard deviation of AFK, KIN and SCS for AB were 573.6 ± 178.5days, 418.8 ± 125.5days and 4.67 ± 2.23 Log2, respectively. The heritabilities (h2) and standard errors of AFK, KIN and SCS for AB were 0.28 ± 0.02, 0.04 ± 0.02 and 0.22 ± 0.01, respectively. The h2 ranged from 0.15 (SA) to 0.37 (NU) for AFK, from 0.04 (AB) to 0.10 (AL) for KIN, and from 0.11 (TO) to 0.26 (LM and ND) for SCS. Genetic correlations between AFK and KIN and between AFK and SCS for AB were positive and weak (0.07 and 0.12, respectively) but significant (P < 0.01). Genetic correlations between SCS and KIN were significant (P < 0.01) for all the breeds and ranged from -0.15 (NU) to 0.44 (AL). Genetic correlations between AFK and SCS in the NU and AL breeds were similar (approximately 0.21). A positive genetic trend was found for KIN in the SA breed, which caused an increase in the number of days between consecutive kiddings. The genetic trend of SCS for the NU, AL and ND breeds was negative and decreased annually, which is beneficial for producers. These first results show the intensity and direction of some favorable/unfavorable relationships between AFK or KIN and SCS Log2 in some U.S. goat genetic groups.

Population viability analysis on the Iranian endangered goat breeds

Iranian goat breeds, including Markhoz, Adani, and Najdi, are considered as endangered populations because of the downward trend of breeding goats and flocks, habitat loss, using other breeds in reproduction, and the presence of survival-threatening factors. This research aims to investigate the current status and provide the necessary information to conserve these three endangered populations. For this purpose, the census data and the required parameters were collected from the available resources and flocks during the years of the project implementation. Then, two scenarios, the past and future dynamics of the population assuming continuing existing conditions, were simulated using the population viability analysis (PVA) method. Finally, the sensitivity analyses of parameters affecting the viability criteria were done. PVA was conducted using the Vortex simulation program version 10.5.6. The past dynamics simulation of the population showed that the biological parameters and assumptions used in the model have valid and reliable values and frequencies, and they can be used in the simulation of different management scenarios. Based on this scenario, the inbreeding rates (∆F) for the Markhoz and Adani populations have reached above the critical level of 0.5%. The future dynamics simulation of the population showed that the process of increasing inbreeding and decreasing genetic diversity would accelerate, and the breeds' extinction probability would start in 2026, 2033, and 2031 for Markhoz, Adani, and Najdi, respectively. The mean time to the first extinction was also 15.4 ± 2.53, 26.68 ± 3.75, and 13.96 ±1.78 years, respectively. The sensitivity analyses indicated that adult does overharvest, the frequency of feed limitation, and competition with other breeds had the highest effect on the population viability criteria of the investigated breeds. Markhoz, Najdi, and Adani goats need urgent conservation measures. The results obtained in this research may prepare a suitable framework for providing conservation programs.

Impacts of Climate Change on Livestock Productivity and Adaptation Strategies among Smallholder Farmers in Ethiopia: A Review on Climate Smart Livestock Production

Ethiopia has the largest livestock number in Africa. Nevertheless, the productivity of this sector faces susceptibility to the effects of climate change and associated risks. Therefore, it is imperative to conduct further research and gather data to gain a deeper understanding of these impacts. This understanding will facilitate the design of effective adaptation and mitigation measures, allowing Ethiopia to effectively respond to the challenges posed by climate change and variability. The primary aim of this article is to provide a review of the impacts of climate change on livestock productivity and summarize adaptation strategies employed by small-scale farmers in Ethiopia. The study highlights an increasing trend in the intensity and frequency of drought occurrences in the country, particularly in pastoralist and agro-pastoralist regions. These trends exacerbate climate-related issues such as limited access to rainfall and water, reduced availability and quality of animal feed, heightened risks of animal diseases, and increased heat stress, resulting in diminished livestock performance. Consequently, collaboration on adaptation programs among stakeholders is crucial to bolster the traditional coping mechanisms of pastoralists and agro-pastoralists in response to the adverse effects of climate change. Additionally, comprehensive research is needed to identify and select camel and goat breeds specifically suited for drought-prone areas, serving particular purposes.

Effect of Season on the Scrotum, Seminal Characteristics, Spermatozoa Integrity and Sexual Behavior in Ganjam Buck

Background: Bucks raised for breeding purposes receive no special attention and are raised at random without following the correct selection process. Besides, the fertility of the male bucks is never addressed. Goat breeders have noted a sharp decline in the reproductive efficiency of Ganjam goats, associated with a lengthened kidding interval, fewer successful pregnancies and decreased twining frequency. The measurement of male fertility parameters is crucial for estimating the breeding performance of any species since males contribute 50% of the reproductive efficiency. Methods: A total of 120 Ganjam bucks of different age groups reared by the local farmers of Rambha, Khalikote and Chhatrapur areas of Ganjam district were used for the present study. A total of six healthier bucks of age 1.5(B1), 2(B2), 2.5(B3), 3.5(B4), 4(B5) and 4.5(B6) years were procured from the Farmers for the experimental purpose. For study on effect of season on the semen qualities, twelve ejaculates from each buck were collected, six ejaculates between the month of July to August (season 1) and six ejaculates during October to November (Season 2). Result: The scrotal length, scrotal width, scrotal circumference have highly significant positive degree of association with Flehmen’s reaction and Libido score and all the parameters exhibited highly significant negative degree of association with reaction time. Moreover, the scrotal length, scrotal width and scrotal circumference had significantly higher positive degree of association with colour, volume of the semen and negative degree of association with MBRT. Further, the sperm concentration revealed a highly significant degree of association with scrotal length, scrotal width, scrotal circumference, livability % and acrosome integrity and a significant degree of association with mass activity. Livability % of the semen samples of the six bucks revealed a highly significant degree of association with scrotal length, scrotal width, scrotal circumference, sperm concentration and acrosome integrity.

Genomic analysis uncovers novel candidate genes related to adaptation to tropical climates and milk production traits in native goats

BackgroundSince domestication, both evolutionary forces and human selection have played crucial roles in producing adaptive and economic traits, resulting in animal breeds that have been selected for specific climates and different breeding goals. Pakistani goat breeds have acquired genomic adaptations to their native climate conditions, such as tropical and hot climates. In this study, using next-generation sequencing data, we aimed to assess the signatures of positive selection in three native Pakistani goats, known as milk production breeds, that have been well adapted to their local climate.ResultsTo explore the genomic relationship between studied goat populations and their population structure, whole genome sequence data from native goat populations in Pakistan (n = 26) was merged with available worldwide goat genomic data (n = 184), resulting in a total dataset of 210 individuals. The results showed a high genetic correlation between Pakistani goats and samples from North-East Asia. Across all populations analyzed, a higher linkage disequilibrium (LD) level (– 0.59) was found in the Pakistani goat group at a genomic distance of 1 Kb. Our findings from admixture analysis (K = 5 and K = 6) showed no evidence of shared genomic ancestry between Pakistani goats and other goat populations from Asia. The results from genomic selection analysis revealed several candidate genes related to adaptation to tropical/hot climates (such as; KITLG, HSPB9, HSP70, HSPA12B, and HSPA12B) and milk production related-traits (such as IGFBP3, LPL, LEPR, TSHR, and ACACA) in Pakistani native goat breeds.ConclusionsThe results from this study shed light on the structural variation in the DNA of the three native Pakistani goat breeds. Several candidate genes were discovered for adaptation to tropical/hot climates, immune responses, and milk production traits. The identified genes could be exploited in goat breeding programs to select efficient breeds for tropical/hot climate regions.

Early growth performance in the Murciano-Granadina goats: insights from genetic and phenotypic analyses

Abstract This study investigates the genetic and phenotypic aspects of early growth performance in the Murciano-Granadina goat breed, using data collected between 2016 and 2022 from a private dairy farm in Ghale-Ganj city, located in the southern area of Kerman province, Iran. Pedigree and data information were collected on several early body weight traits, including birth weight (BW), weaning weight (WW), average daily gain (ADG), Kleiber ratio (KR) and growth efficiency from birth to weaning (GE). Nine univariate animal models included direct additive genetic effects and different combinations of maternal effects were compared by using Akaike information criterion (AIC). Among the tested models, the best genetic analysis model for BW, included direct additive, maternal additive, maternal permanent and maternal temporary environmental effects. The best model for ADG, KR and GE included direct additive, maternal permanent and litter effects. For WW, the best model was determined to be one that included direct additive and maternal additive genetic effects. The estimated direct heritabilities were low values of 0.04, 0.07, 0.08, 0.05 and 0.07 for BW, ADG, KR, GE and WW, respectively. The estimates of genetic correlations among the studied traits were positive and low to high in magnitude which ranged from 0.11 for BW-KR to 0.91 for BW-GE. The phenotypic correlations ranged from 0.03 for KR-WW to 0.87 for ADG-KR. The positive correlations observed among the studied growth traits of the Murciano-Granadina goat breed indicate no negative genetic or phenotypic changes associated with selection for these traits.

Influence of stimulating basics on the level of iron in the blood of Saanen kids

Goat milk is becoming increasingly attractive for the food sector due to some advantages compared to cow milk: it has higher fat content, protein levels, magnesium, potassium, phosphorus, cobalt, vitamins B1, B2, C, and some amino acids. The Saanen breed is the most attractive for farms since, along with the qualitative superiority of milk, this breed is characterized by increased milk yield. Currently, the main goal of research in goat breeding is to preserve and improve animals’ genetic potential under intensive exploitation conditions when any environmental factor (incredibly inadequate feeding) can negatively affect the productive qualities of animals. The period of their prenatal and early postnatal ontogenesis is especially significant. One of the critical indicators of metabolic processes in the body of young animals is iron, which enhances oxidative processes and promotes the reproduction of erythrocyte hemoglobin. The study showed the influence of the age factor on an increase in the concentration of iron in the blood and better absorption of iron in the presence of copper in the body. The effectiveness of using an iron-containing preparation in combination with protein hydrolysate, a source of protein necessary for the formation of enzymes and hormones involved in regulating iron metabolism in the body, has been noted.

A bibliometric analysis of the literature on goat breeding

Background Bibliometric analysis plays an essential role in scientific research. However, some research areas still have several gaps. This is also the case in the animal sciences. Therefore, the status of current studies can be analyzed to provide guidance for future research through scientific studies that will be conducted. The aim of this study was to perform a bibliometric analysis of goat breeding to evaluate current trends, gaps in knowledge, and future perspectives. Methods A large dataset was extracted from the Web of Science (WoS), a popular database for scientific studies. According to the WoS, we used 1000 scientific papers (1991–2023) that contained articles, book chapters, data papers, proceedings papers, meeting abstracts, and notes. All bibliometric analyses were performed using the bibliometrix package. Results and Conclusions In 2023, there were 34 studies available. Average citations per year showed that the highest average citation count occurred in 2006 (31.35). In total, 265 scientific journals in the WoS database have published goat breeding studies. Small Ruminant Research led to the remaining 145 articles. In conclusion, scientific studies on goat breeding have become popular in recent years. The authors may gravitate towards popular journals in the future.

Development and verification of a 10K liquid chip for Hainan black goat based on genotyping by pinpoint sequencing of liquid captured targets

BackgroundChina has thousands years of goat breeding and abundant goat genetic resources. Additionally, the Hainan black goat is one of the high-quality local goat breeds in China. In order to conserve the germplasm resources of the Hainan black goat, facilitate its genetic improvement and further protect the genetic diversity of goats, it is urgent to develop a single nucleotide polymorphism (SNP) chip for Hainan black goat.ResultsIn this study, we aimed to design a 10K liquid chip for Hainan black goat based on genotyping by pinpoint sequencing of liquid captured targets (cGPS). A total of 45,588 candidate SNP sites were obtained, 10,677 of which representative SNP sites were selected to design probes, which finally covered 9,993 intervals and formed a 10K cGPS liquid chip for Hainan black goat. To verify the 10K cGPS liquid chip, some southern Chinese goat breeds and a sheep breed with similar phenotype to the Hainan black goat were selected. A total of 104 samples were used to verify the clustering ability of the 10K cGPS liquid chip for Hainan black goat. The results showed that the detection rate of sites was 97.34% -99.93%. 84.5% of SNP sites were polymorphic. The heterozygosity rate was 3.08%-36.80%. The depth of more than 99.4% sites was above 10X. The repetition rate was 99.66%-99.82%. The average consistency between cGPS liquid chip results and resequencing results was 85.58%. In addition, the phylogenetic tree clustering analysis verified that the SNP sites on the chip had better clustering ability.ConclusionThese results indicate that we have successfully realized the development and verification of the 10K cGPS liquid chip for Hainan black goat, which provides a useful tool for the genome analysis of Hainan black goat. Moreover, the 10K cGPS liquid chip is conducive to the research and protection of Hainan black goat germplasm resources and lays a solid foundation for its subsequent breeding work.

Prolactin gene (C576A) polymorphism is associated with milk production performance in crossbred Anglo-Nubian dairy goats

Crossbred Anglo-Nubian goats (Anglo-Nubian × native goat breeds), are valued for exceptional milk production and adaptability. In-depth research on these goats is imperative to propel advancements in dairy goat breeds and enhance milk production efficiency within the region. The present study aims to assess polymorphism in prolactin (C576A) gene and determine the influence of different prolactin (PRL) genotypes to milk yield performance in crossbred Anglo-Nubian dairy goats raised from Awang, Opol, Misamis Oriental and Talay, Dumaguete City, Negros Oriental. Genomic DNA was extracted from hair follicles using commercial DNA extraction kit and PCR-RFLP was performed for the genotyping of the C576A polymorphism located in exon 5 of goats’ prolactin gene using Eco241 restriction enzyme. Genotypic frequencies of 0.56 for AA, 0.44 for AB, while allelic frequencies of 0.78 for A, and 0.22 for B were recorded. All populations followed the Hardy–Weinberg principle, except for dairy goats from Farm A located in Opol, Misamis Oriental. A two-way factorial (2 × 4) in a Randomized Complete Block Design was used to evaluate the relationship between genotypes and milk yield performance. AB genotype goats produced significantly higher milk yield traits (average daily milk yield and total milk production) than AA genotype, an indication that the polymorphism in the caprine PRL (C576A) gene influenced milk yield performance in the population of studied crossbred Anglo-Nubian goats. These results have to be validated in other dairy goat breeds.

Using Metabolomics to Understand Selective Breeding of Goats that Enables Tolerance of Plant Secondary Compounds

The objective of this study was to determine aspects of liver function in goats with a divergent propensity to consume juniper ( Juniperus spp.), a terpene -containing woody plant. Goats represent a significant contribution to sustainable food production and local economies worldwide. Climate change in arid and semiarid regions has led to a transition of grasslands to woodlands. Goats ( Capra hircus) are adapted to utilize woody plants as a food source; however, herbivory can be limited by plant secondary compounds (e.g., monoterpenes). Estimated breeding values (EBV) have been determined for domestic goats based on their propensity to consume above (H: EBV = 13.1 ± 0.2) or below (L: EBV = −14.8 ± 0.5) the herd average for proportion of juniper in the diet of free-ranging animals. To investigate the physiological and metabolic mechanisms enabling increased intake and tolerance of juniper, H and L adult male Spanish goats within young (2-yr-old) and old (3-5-yr-old) groups that spent their life grazing pastures containing juniper were randomly selected from our research herd. Twenty goats (n = 5 of each age and juniper consumption combination) were weighed and harvested. Serum samples and multiple organs (i.e., liver, kidney, heart, spleen, adrenal, testis) were collected, weighed, and preserved for analyses. With an initial focus on the liver, samples (200 mg) were submitted to Metabolon, Inc. (Morrisville, NC) to identify metabolites by ultra-high-performance liquid chromatography/tandem high resolution/accurate mass spectrometry chromatography methods. Data were analyzed using the PROC MIXED procedure of Statistical Analysis System (SAS Institute Inc., Cary, NC). Goat body weight (BW), relative organ weight (g/kg BW), and serum constituents were analyzed using a model that included age (O, Y), treatment (H, L), and the age x treatment interaction. Animals were the experimental unit and error degrees of freedom = 16. Statistical significance was determined at P ≤ 0.05. Multiple differences in metabolites of the lipid and protein pathways were identified. For example, the relative intensity of 19 monoacylglycerol metabolites was greater (P &lt; 0.05) in H than L goats. This report, however, focuses on the gamma-glutamyl amino acid (GGAA) pathway due to its role in diagnosis of liver dysfunction. Relative liver weight (g/kg BW) was numerically greater (P = 0.15) in H (15.80 ± 0.75) than L (14.58 ± 0.39) goats. Serum gamma glutamyl transferase (U/L) was within the clinically normal range but was greater (P &lt; 0.0001) in H (59.2 ± 2.1) versus L (44.3 ± 2.2), and in Y (55.3 ± 2.9) versus O (48.2 ± 3.2) goats. Of the 18 GGAAs identified from the GGAA super- and sub-pathways, the relative intensities of 11 metabolites were greater (P &lt; 0.05) in liver from H than L; 8 were greater in HY versus LY, and only 2 were greater in OH versus OL goats. The increased expression of serum and liver components of the GGAA pathway for the goats with a positive estimated breeding value for juniper consumption may be indicative of metabolic mechanisms and biomarkers associated with increased tolerance of plant secondary compounds. Texas A&amp;M AgriLife Research. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Grading system to support the Kacang goat breeding program

This study aims to make it easier for breeders to determine which Kacang goats have advantages based on the phenotypic parameters (chest diameter). This study used a survey method using questionnaires and morphometric measurements as the basis for grading. The results showed that male Kacang goats aged 1.5 and 2 years had an average chest diameter of 63.52 ± 5.82 cm and 67.4 ± 2.8 cm, respectively, with coefficients of variance of 10.92% and 24.07%. The mean chest diameter of female Kacang goats aged 1.5 and 2 years were 70.10 ± 6.12 cm and 72.6 ± 1.85 cm, respectively, with coefficients of variance of 11.45% and 39.14%. The best chest diameters based on SNI on males aged 1.5 and 2 years were 8 and 2, respectively. Females aged 1.5 and 2 years were 20 and 5, respectively, from the total recorded data.

Selection signatures and landscape genomics analysis to reveal climate adaptation of goat breeds

Goats have achieved global prominence as essential livestock since their initial domestication, primarily owing to their remarkable adaptability to diverse environmental and production systems. Differential selection pressures influenced by climate have led to variations in their physical attributes, leaving genetic imprints within the genomes of goat breeds raised in diverse agroecological settings. In light of this, our study pursued a comprehensive analysis, merging environmental data with single nucleotide polymorphism (SNP) variations, to unearth indications of selection shaped by climate-mediated forces in goats. Through the examination of 43,300 SNPs from 51 indigenous goat breeds adapting to different climatic conditions using four analytical methods: latent factor mixed models (LFMM), F-statistics (Fst), Extended haplotype homozygosity across populations (XPEHH), and spatial analysis method (SAM), A total of 74 genes were revealed to display clear signs of selection, which are believed to be influenced by climatic conditions. Among these genes, 32 were consistently identified by at least two of the applied methods, and three genes (DENND1A, PLCB1, and ITPR2) were confirmed by all four approaches. Moreover, our investigation yielded 148 Gene Ontology (GO) terms based on these 74 genes, underlining pivotal biological pathways crucial for environmental adaptation. These pathways encompass functions like vascular smooth muscle contraction, cellular response to heat, GTPase regulator activity, rhythmic processes, and responses to temperature stimuli. Of significance, GO terms about endocrine regulation and energy metabolic responses, key for local adaptation were also uncovered, including biological processes, such as cell differentiation, regulation of peptide hormone secretion, and lipid metabolism. These findings contribute to our knowledge of the genetic structure of climate-triggered adaptation across the goat genome and have practical implications for marker-assisted breeding in goats.

Metabolic response of endangered goats fed spineless cactus associated with Tifton-85 hay or Maniçoba hay

The aim of this study was to evaluate the effects of replacement of Tifton-85 hay by maniçoba hay, in spineless cactus-based diets, on markers of energy, protein, mineral metabolism and serum enzyme activity of Moxotó goats. Sixteen uncastrated male Moxotó breed goats, with 20 months old and initial body weight of 21.7 ± 3.92 kg were used in a randomized blocks design, using the initial weight as the criterion for the formation of blocks, with two treatments and eight replicates per treatment. The experimental treatments consisted of two diets: diets containing Miúda spineless cactus associated with Tifton-85 hay (1) or maniçoba hay (2), with roughage:concentrate ratio of 70:30. Blood samples were collected fortnightly (baseline, 15 days, 30 days and 45 days) during the feedlot period. There was a higher serum concentration of total protein and fructosamine (P &lt; 0.05) in goats that received maniçoba hay. However, there was higher serum gamma-glutamyl transferase (GGT) enzyme activity in goats that ingested Tifton-85 hay (P &lt; 0.05). Concerning to the profile of blood metabolites in the different fortnightly collections, it was possible to verify a significant effect for urea, fructosamine, GGT enzyme activity, K and Cl levels (P &lt; 0.05). In conclusion, Tifton-85 hay and maniçoba hay combined with spineless cactus does not cause metabolic disorders in feedlot native goats.

Genomic Inbreeding and Runs of Homozygosity Analysis of Cashmere Goat.

Cashmere goats are valuable genetic resources which are famous worldwide for their high-quality fiber. Runs of homozygosity (ROHs) have been identified as an efficient tool to assess inbreeding level and identify related genes under selection. However, there is limited research on ROHs in cashmere goats. Therefore, we investigated the ROH pattern, assessed genomic inbreeding levels and examined the candidate genes associated with the cashmere trait using whole-genome resequencing data from 123 goats. Herein, the Inner Mongolia cashmere goat presented the lowest inbreeding coefficient of 0.0263. In total, we identified 57,224 ROHs. Seventy-four ROH islands containing 50 genes were detected. Certain identified genes were related to meat, fiber and milk production (FGF1, PTPRM, RERE, GRID2, RARA); fertility (BIRC6, ECE2, CDH23, PAK1); disease or cold resistance and adaptability (PDCD1LG2, SVIL, PRDM16, RFX4, SH3BP2); and body size and growth (TMEM63C, SYN3, SDC1, STRBP, SMG6). 135 consensus ROHs were identified, and we found candidate genes (FGF5, DVL3, NRAS, KIT) were associated with fiber length or color. These findings enhance our comprehension of inbreeding levels in cashmere goats and the genetic foundations of traits influenced by selective breeding. This research contributes significantly to the future breeding, reservation and use of cashmere goats and other goat breeds.

MiR-2284b regulation of α-s1 casein synthesis in mammary epithelial cells of dairy goats

The lactation character of dairy goats is the most important characteristic, and milk protein is an important index to evaluate milk quality. Casein accounts for more than 80% of the total milk protein in goat milk and is the main component of milk protein. Using GMECs (goat mammary epithelial cells) as the research object, the CHECK2 vector of the CSN1S1 gene and the overexpression vector of pcDNA 3.1 were constructed, and the mimics of miR-2284b and the interfering RNA of CSN1S1 were synthesized. Using PCR, RT–qPCR, a dual luciferase activity detection system, EdU, CCK8, cell apoptosis detection and ELISA detection, we explored the regulatory mechanism and molecular mechanism of miR-2284b regulation of αs1-casein synthesis in GMECs. miR-2284b negatively regulates proliferation and apoptosis of GMECs and αs1-casein synthesis. Two new gene sequences of CSN1S1 were discovered. CSN1S1-1/-2 promoted the proliferation of GMECs and inhibited cell apoptosis. However, it had no effect on αs1-casein synthesis. MiR-2284b negatively regulates αs1-casein synthesis in GMECs by inhibiting the CSN1S1 gene. These results all indicated that miR-2284b could regulate αs1-casein synthesis, thus playing a theoretical guiding role in the future breeding process of dairy goats and accelerating the development of dairy goat breeding.

A Mitochondrial DNA-based Molecular Phylogenetics Study of the Mahalli Goat as a New Animal Genetic Resource in Southern Anatolia in Turkey

Background: The Karacadağ Mountains are of extinct volcanic origin and cover a large area in the triangle between Diyarbakır (Center, Nar and Ergani districts), Şanlılurfa (Siverek and Viranşehir districts) and Mardin (Derik district). Agricultural activities are limited due to the stony structure of the region; therefore, small animal husbandry is intensively practiced in the villages of the region. Mahalli goats, which are morphologically new domestic goats, are distinguished from other domestic goats by their spiral horn structure. They are not only resistant to cold weather conditions, but also resistant to diseases. Therefore, present study aimed to determine the genetic diversity and phylogenetic relationships of Mahalli goats in Diyarbakır region. Methods: The study was carried out at Şanlıurfa, Harran University of Agricultural Faculty, Laboratory of Animal Biotechnology and Molecular Genetics, in 2019-2020. Out of 24 Mahalli goats, forward and reverse primers were used to amplify the 598 bp region over the mtDNA D-loop region encompassing the 481 bp most variable region (HVR1). Analysis of goat D-loop sequences revealed 481 regions of 0.378 G+C, 97 polymorphic regions and 19 haplotypes. The phylogenetic analysis of Mahalli goats was carried out using molecular genetic techniques. Result: As a result of data analysis, both haplotype diversity (HD) and nucleotide diversity (ND) were calculated to be 1.000±0.0039 and 0.0375±0.00209, respectively. The genetic distance between the haplotypes of Mahalli goat ranged from 0.003 to 0.087 and the genetic distance between the haplotypes of Mahalli goat and other domestic goat breeds in Turkey ranged from 0.016 to 0.027. All 19 haplotypes used in this study were represented in the lineage A phylogenetic tree.

Состояние отечественной отрасли козоводства на современном этапе развития России

The article analyzes the state of the goat breeding industry in Russia from 2007 to 2022. Based on official data from the Federal State Statistics Service and data from the All-Russian Research Institute of Breeding, presented in the public domain, an assessment of the state of the goat breeding industry in Russia was carried out and the main trends and patterns were identified. As part of the study, it was revealed that goat breeding in Russia is mainly concentrated in small forms of farming and currently there are no prospects for scalability, while the number of goats is decreasing annually. The leader among federal districts in terms of livestock is the Siberian Federal District, and among the subjects of the Federation the undisputed leader is the Republic of Tyva. Dairy goat farming ranks third in the world in milk production, behind cows and buffaloes. In Russia, there is a clear trend towards increasing milk production. The leader in goat milk production is the Leningrad region. The analysis of modern domestic goat breeding made it possible to formulate the main problems that impede the effective development of the industry in the country and outline possible ways to solve them.