Aim: The aim of this study was to investigate the effect of training for breast cancer and self-breast examination on university students’ anxiety level and health belief model. Methods: This study is a quasi-experimental study with one group pretest-posttest design. The sample of the study was selected by convenience sampling method. The target population of this study was the female students attending at a public university from eastern Anatolia region of Turkey and the sample was composed of those who accepted to participate in the study. 126 students in total participated in the study on a voluntary basis. “Personal Information Form”, and “Champion's Health Belief Model Scale” and “Hamilton Anxiety Rating Scale” were used as data collection tools. Results: It was determined that 84.1% of the students evaluated their health as good and 93.7% of them did not have a breast cancer history in their family. After training, it was determined that students’ health beliefs were improved, and their anxiety levels was decreased. When the subscale total scores of the health belief model scale were examined, it was observed that the perceived susceptibility and perceived confidence sub-dimension scores increased significantly after the training, and the perceived barrier score decreased significantly (p<0.05). Conclusion: As a result of the study, it was determined that breast cancer and breast self-examination training had an effect on students’ anxiety level and health beliefs. After the training, while students’ anxiety level decreased, their health beliefs was improved.