Breast Bone Length Research Articles

Research Note: Association of insulin-like growth factor 1 receptor gene polymorphism with production performance in Savimalt and French Giant meat-type quails

This study aimed to investigate the association of insulin-like growth factor 1 receptor (IGF-1R) gene single nucleotide polymorphisms (SNPs) with growth traits and carcass traits of quail by PCR amplification and direct sequencing technology. Genomic DNA was extracted from blood samples collected from 49 female French Giant (FG) quails and 36 female Savimalt (SV) quails as part of this study. Growth traits and carcass traits were measured and assessed for IGF-1R gene analysis in the 2 meat-type quail strains. The results showed that 2 SNPs (A57G and A72T) of the IGF-1R gene were detected in the 2 quail strains. The A57G (P=0.002) and A72T (P=0.026) were significantly associated with breastbone length (BBL) in FG. Whereas A57G was significantly associated with chest weight (CW, P=0.004), BBL (P=0.009), and body length (BL, P=0.009) in SV, while A72T was significantly associated with BBL (P=0.014) and BL (P=0.028) in SV. Haplotypes based on these 2 SNPs showed significant effects on BBL in FG strain (P=0.000), and they also had significant effects on CW (P=0.007), BBL (P=0.004), and BL (P=0.001) in SV strain. Additionally, A57G was significantly associated with liver rate (LR) in FG strain (P=0.017). A72T showed significant associations with dressed carcass weight (DCW, P=0.048) and breast muscle weight (BMW, P=0.018) in FG strain. A57G was significantly associated with DCW (P=0.048), whole net carcass weight (WNCW, P=0.048), BMW (P=0.036), and liver muscle rate (LMR, P=0.003) in SV strain. Haplotypes also displayed significant effects on BMW (P=0.029) and LMR (P=0.010) in FG strain. These findings indicated that the IGF-1R gene could serve as a valuable molecular genetic marker for enhancing growth traits and carcass traits in meat-type quails.

Research Note: Association of LEPR gene polymorphism with growth and carcass traits in Savimalt and French Giant meat-type quails

The leptin receptor (LEPR) gene is a member of the class I cytokine receptor family, which plays an important role in weight regulation, fat accumulation and neuroendocrine function in animals. This study aimed to explore the association of single nucleotide polymorphisms (SNPs) of the LEPR gene with growth and carcass traits in meat-type quail by PCR amplification and DNA direct sequencing. In this study, genomic DNA was extracted from blood samples of 36 female Savimalt (SV) quails and 49 female French Giant (FG) quails. Growth traits (measured at 3 or 5 wk) and carcass traits (measured at 5 wk) were used for LEPR gene association analysis. The results showed the existence of 9 SNPs (T81C, G90T, C187A, A191G, A219G, G258A, C286T, G346A, and G373A) of the LEPR gene in the 2 quail strains. The statistical analyses indicated that these SNPs of LEPR gene was significantly associated with shank circumference (SC), shank length (SL), breastbone length (BBL), heart rate (HR), and whole net carcass rate (WNCR) of FG (P < 0.05); chest width (CW), body length (BL), leg muscle rate (LMR), whole net carcass rate (WNCR), and heart rate (HR) of SV (P < 0.05). While haplotypes showed significant effect on SL, BBL, heart weight (HW), WNCR, and HR of FG (P < 0.05). Therefore, the LEPR gene may serve as a molecular genetic marker for improving growth and carcass traits in quails.

Research Note: Polymorphisms of gonadotrophin-releasing hormone gene and their association with growth traits in quail (Coturnix Coturnix)

This study aimed to identify polymorphisms of gonadotropin-releasing hormone (GnRH) gene and their association with growth traits in quail by PCR and direct sequencing. Genomic DNA was extracted from quail blood samples of 36 from Savimalt (SV) and 49 from French Giant (FG). Growth traits were measured and used for candidate gene analysis, as body weight (BW), shank length (SL), chest width (CW), chest depth (CD), breastbone length (BBL), body length (BL), and shank circumference (SC). The results showed that a total of 20 SNPs were detected in GnRH gene, whereas 8 SNPs were significantly associated with growth traits (P < 0.05). The T215C, G279A, C458T, A520G, and C547G were significantly associated with SL at 3 wk of age in the FG strain, whereas A583T was significantly related to BBL and BL, and C591T was significantly related to SL, BBL, and BL, whereas A592G was significantly correlated with SL, CW, CD, BBL, and BL (P < 0.05). The 8 SNPs were significantly related to CW, CD, and BBL at 3 wk of age in the SV strain, whereas A583T, C591T, and A592G were significantly associated with BW (P < 0.05). The G279A showed significant correlations with SL at 5 wk of age in FG, whereas A583T showed significant associations with SC in FG, and C591T was significantly associated with BW and SC in FG, whereas A592T was significantly related to BW, SL, and CD in FG (P < 0.05). The T215C, G279A, C458T, A520G, and C547G were significantly correlated with BW, CW, BBL, and BL at 5 wk of age in SV, whereas A583T, C591T, and A592G were significantly related to BW, SL, CW, BBL, and BL (P < 0.05). Haplotypes based on 8 SNPs showed significant correlation with BW, SL, CW, CD, BBL, BL, and SC in FG (P < 0.05). In conclusion, the GnRH gene could be used as a molecular genetic marker to provide theoretical foundation to improve growth traits in quail.

Supplementation of Threonine and Tryptophan in Feed with Different Crude Protein Levels on Body Dimensions of Native Chickens in the Grower Phase (6 - 14 Weeks)

The purpose of this study is to find out the influence of feeding different levels of protein in feed on the dimensions of the body of the village chicken grower phase (6-14 weeks), to find out the addition of amino acids treonine and tryptophan in feed to the dimensions of the body of the village chicken grower phase (6-14 weeks) and to know the interaction of protein leveling and the addition of amino acids treonine and tryptophan in feed to the dimensions of the body of the village chicken grower phase (6-14 weeks). This research has been conducted in Sasi Subdistrict, Kefamenanu City District, North Central Timor Regency for 8 weeks from October-December 2020. This study used 6 weeks old native chickens as many as 120 heads. The method used in this study is a complete random design of factorial patterns of 2 x 3 i.e. 2 levels of amino acids and 3 levels of coarse protein. Treatment given A1C1 (treonine 0.50% + tryptophan 0.15% + protein 15%), A1C2 (treonine 0.50% + tryptophan 0.15% + protein 17%), A1C3 (treonine 0.50% + tryptophan 0.15% + protein 19%), A2C1 (treonine 0.75% + tryptophan 0.20% + protein 15%), A2C2 (treonine 0.75% + tryptophan 0.20% + protein 17%), A2C3 (treonine 0.75% + tryptophan 0.20% + protein 19%). The variables observed in this study were weight, chest circumference, body length, length of sternum, femur length, tibia length, shank length, shank circumference and finger length. The data obtained was analyzed using variance analysis and multiple distance tests. Statistical analysis showed that crude protein levels had an unreal effect on chest circumference, body length, breastbone length, femur length, tibia length, shank length, shank circumference and finger length. Levels of amino acids (treonine + tryptophan) have a noticeable effect on weight, chest circumference, body length, length of sternum, tibia length, shank length and finger length. There is an interaction between amino acids (treonine + tryptophan) and crude protein levels against chest circumference, femur length, shank circumference and finger length.

Comparative study of hatchability rate and egg quality between different strains of Japanese Quail (Coturnix japonica)

The objectives of this study were to determine morphology of Japanese quail strains andhatchability rate and egg quality between commercial strain which is Poultry Progress Institute(IKTA) strain and White Texas strains of Japanese Quail (Coturnix japonica) rear in Malaysia. Atotal of 300 quails were randomly picked and the breastbone length, chest girth, body weight,body length, shank length and drum length were measured for the morphology. Then, 270 eggs ofCommercial and White Texas strains were used to test the hatchability rate (n=150) and eggquality (n=120). The ratio of the parents of the quails between male and female of each type ofstrain were 1:3. The cleanliness, shape, weight, and texture of the eggshell were observed,weighed and measured. The eggs were incubated for 17 days (37.6 °C, 65% humidity). The shapeindex, egg weight, egg volume and eggshell surface were weighed and measured for the exteriortrait of eggs. For interior trait, the albumen index, Haugh units, and internal quality units (IQU)were measured. Significant findings were observed in all of the features (P&lt;0.05), except for thewing length in morphology. For hatchability rate, there was a high significant in the percentage ofhatchability of incubated eggs, hatchability of fertile eggs and smoothness texture of the eggshell.In egg quality there was high significant in Haugh unit but low significant internal quality unit.The significant variability might be due from the genetic information that inherited from theparents. The IKTA and White strains were not the same in morphology, hatchability rate andinterior trait of eggs but same exterior trait of eggs. In conclusion, this research is important ingiving information about Japanese quail strain in Malaysia and more genetic study should be donein the future to determine good strain and increase understanding about quail strain.

The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia

Abstract. Abinawanto, Zulistiana T, Lestari R, Dwiranti A, Bowolaksono A. 2021. The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia. Biodiversitas 22: 3145-3155. Ayam Ketawa, a variation of Gallus gallus domesticus Gallus gallus domesticus, comes from Sidendreng-Rappang (Sidrap) District, South Sulawesi, Indonesia. Apart from Sidrap, ayam ketawa farms are also found in Bangkalan District, on the island of Madura, Indonesia. Ayam ketawa from Bangkalan District often wins national level contests. Therefore, the research was carried out with the aim of knowing the genetic diversity of ayam ketawa in Kamal Subdistrict, Socah Subdistrict, and Burneh Subdistrict, based on bioacoustic, morphometric, and barcoding DNA analysis. The studied ayam ketawa consists of dangdut and slow types. The longest duration (6.00 ± 3.0) seconds was shown by ayam ketawa from Kamal Subdistrict, while the highest number of syllables (7.20 ± 5.80) came from Socah Subdistrict. The results of the Mann-Whitney test showed that eight morphological characters were significantly different (P &lt;0.05) between ayam ketawa population in Kamal and Burneh subdistricts, namely body weight, femur length, shank length, shank circumference, wing length, comb height, third finger length, and breastbone length. Four morphological characters differed significantly (P &lt;0.05) between ayam ketawa population in Kamal and Socah subdistricts, namely shank length, wing length, third finger length, and sternum length. Three morphological characters were significantly different (P &lt;0.05) between the Burneh and Socah subdistricts population, namely body weight, femur length, and shank circumference. The analysis results of DNA Barcoding gene of the Cytochrome Oxidase subunit 1 (CO1) showed a genetic distance ranging from 0.025 to 1.872.

Polymorphism analysis of myogenin gene in meat quail (Coturnix coturnix)

Results demonstrated that in meat quail, three genotypes (AA, BB and AB) were detected at locus A and B in MyoG gene. Locus A is significantly correlated with weight and body length of meat quail (P&lt;0.05). Locus B is significantly correlated with weight, breastbone length and body length of meat quail (P&lt;0.05). The MyoG gene can be used for marker-assisted selection of growth traits in meat quails.

Association Analysis Between Polymorphism of Gonadotrophin Releasing Hormone Genes and Growth Traits of Quail (Coturnix Coturnix)

ABSTRACT This study investigated SNP mutation sites of Gonadotrophin releasing hormone (GnRH) gene in China yellow quail, Beijing white quail and Korean quail through PCR amplification and DNA sequencing technologies. Moreover, polymorphism of GnRH gene and its association with growth traits of quail were analyzed, aiming to get molecular markers associated to growth traits of quail, which could provide references for breeding of new quail species. According to research results, a total of 14 SNP mutation sites of GnRH were detected in China yellow quail, Beijing white quail and Korean quail, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T and C458T. Except that only 2 genotypes were detected for A209C and C281T in China yellow quail and Beijing white quail, 3 genotypes were detected for all of the remaining 12 SNP mutation sites in three quail species. Of the 14 SNP sites, C71T, A209C, C215T, C281T, C293G, C339T and C458T were significantly associated with body weight (p<0.05), C71T, C108T, C168T, C178T, A184G, C206T, C215T, A252G, C293G, C339T and C458T were significantly associated with shank length (p<0.05), C71T, C215T, C293G and C458T were significantly associated with breastbone length (p<0.05), A209C and C281T were significantly associated with shank circumference (p<0.05).

Detection of CNV in the SH3RF2 gene and its effects on growth and carcass traits in chickens

BackgroundThe SH3RF2 gene is a protein-coding gene located in a quantitative trait locus associated with body weight, and its deletion has been shown to be positively associated with body weight in chickens.ResultsIn the present study, CNV in the SH3RF2 gene was detected in 4079 individuals from 17 populations, including the “Gushi ×Anka” F2 resource population and populations of Chinese native chickens, commercial layers, and commercial broilers. The F2 resource population was then used to investigate the genetic effects of the chicken SH3RF2 gene. The results showed that the local chickens and commercial layers were all homozygous for the wild-type allele. Deletion mutation individuals were detected in all of the commercial broiler breeds except Hubbard broiler. A total of, 798 individuals in the F2 resource group were used to analyze the effects of genotype (DD/ID/II) on chicken production traits. The results showed that CNV was associated with 2-, 6-, 10-, and 12-week body weight (P = 0.026, 0.042, 0.021 and 0.039 respectively) and significantly associated with 8-week breast bone length (P = 0.045). The mutation was significantly associated with 8-week body weight (P = 0.007) and 4-week breast bone length (P = 0.010). CNV was significantly associated with evisceration weight, leg muscle weight, carcass weight, breast muscle weight and gizzard weight (P = 0.032, 0.033, 0.045, 0.004 and 0.000, respectively).ConclusionsCNV of the SH3RF2 gene contributed to variation in the growth and weight gain of chickens.

Comparative analysis on early growth and development of different egg-laying quails

This study was carried out to evaluate growth and development and difference between size and weights of three quail species namely China yellow quail, Beijing white quail and Korea quail. Various parameters in respect of these species like shank length, chest width, chest depth, breastbone length, shank circumference, body length, daily weight gain and weight were recorded under similar feeding conditions. The data so obtained was analysed statistically using SPSS. It was revealed that the body weight, shank length, chest width, chest depth, breastbone length, body length, shank circumference and daily gain of Korea quail are significantly higher (P£0.05) than those of China yellow quail and Beijing white quail. It was concluded the Korea quail grows fastest, followed by China yellow quail and Beijing white quail, successively.

SNP in pre-miR-1666 decreases mature miRNA expression and is associated with chicken performance.

Polymorphisms in miRNA genes could potentially alter various biological processes by influencing the processing and (or) target selection of miRNAs. The rs14120863 (C > G) mutation, which we characterized in a Gushi-Anka F2 resource population, resides in the precursor region of miR-1666. Association analysis with chicken carcass and growth traits showed that the SNP was significantly associated with carcass weight, evisceration weight, breast muscle weight, leg muscle weight, and body weight at 8 weeks of age, as well as some body size indexes including shank girth, chest breadth, breast bone length, and body slanting length, in the Gushi-Anka F2 resource population. Quantitative RT-PCR results showed that miR-1666 expression levels in muscle tissues differed within various genotypes. Experiment in DF1 cells further confirmed that the SNP in miR-1666 could significantly alter mature miRNA production. Subsequently, using dual-luciferase report assay, we verified that miR-1666 could perform its function through targeting of the CBFB gene. In conclusion, the SNP in the precursor of miR-1666 could significantly reduce mature miR-1666 production. It may further affect the function of miR-1666 through the target gene CBFB, hence it is associated with chicken growth traits.

Identification of single nucleotide polymorphisms in the ASB15 gene and their associations with chicken growth and carcass traits.

ASB15 is a member of the ankyrin repeat and suppressor of cytokine signaling box family, and is predominantly expressed in skeletal muscle. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken ASB15 gene. Two single nucleotide polymorphisms (SNPs) (rs315759231 A>G and rs312619270 T>C) were identified in exon 7 of the ASB15 gene using forced chain reaction-restriction fragment length polymorphism and DNA sequencing. One was a missense SNP (rs315759231 A>G) and the other was a synonymous SNP (rs312619270 T>C). The rs315759231 A>G polymorphism was significantly associated with body weight at birth, 12-week body slanting length, semi-evisceration weight, evisceration weight, leg muscle weight, and carcass weight (P < 0.05). The rs312619270 T>C polymorphism was significantly associated with body weight at birth, 4, 8, and 12-week body weight, 8-week shank length, 12-week breast bone length, 8 and 12-week body slanting length, breast muscle weight, and carcass weight (P < 0.05). Our results suggest that the ASB15 gene profoundly affects chicken growth and carcass traits.

Adjacent SNPs in the transcriptional regulatory region of the FADS2 gene associated with fatty acid and growth traits in chickens.

Delta-6 fatty acid desaturases are rate-limiting desaturases involved in metabolic processes of fatty acids, and they are encoded by the FADS2 gene. In the current study, an F2 resource population of Gushi chickens crossed with Anak broilers was used to investigate the genetic effects of the chicken FADS2. Two adjacent single nucleotide polymorphisms (SNPs) (g.4290C>G and g.4291C>A) were identified in the transcriptional regulatory region of the FADS2 gene by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site-PCR-RFLP. Associations between the two SNPs with chicken fatty acid contents and growth traits were determined using linkage disequilibrium, haplotype construction, and association analysis. The two SNPs and their haplotype combinations were significantly associated with linoleic acid (C18:2), α-linolenic acid (C18:3), arachidonic acid (C20:4), body weight (BW)2, BW4, BW6, shank girth (SG)4, and breast bone length 4 (P<0.05). These results suggested that the SNPs of the FADS2 gene affected the content of essential fatty acid in muscle, and played a role in the early-stage growth rate of chickens.

Cloning of TPO gene and associations of polymorphisms with chicken growth and carcass traits

Thyroid peroxidase (TPO), which located on the apical membrane surface of thyrocytes, is the key enzyme involved in thyroid hormone synthesis, mainly catalyses the iodination of tyrosine residues and the coupling of iodotyrosines on thyroglobulin to form thyroxine and triiodothyronine. The objectives of this study were to identify genetic polymorphisms of the chicken TPO gene and to analyze potential association between single nucleotide polymorphisms (SNPs) and growth and carcass traits in chicken. Partial sequences of TPO gene were cloned firstly. The nucleotide sequence was found to have 72 % identity with that of humans. The chicken TPO amino acid sequence was 71 %. Through polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing methods, three novel mutations of the chicken TPO gene were detected in the F2 resource population from Gushi chickens and Anka broilers. The association analysis indicated that all of the three SNPs showed association with chicken growth at different periods. The g.29996C>T polymorphisms was significantly associated with body weight, breast bone length, pectoral angle at 12 weeks, claw weight and leg muscle weight (P < 0.05). In addition, individuals with the TT genotype had higher value for almost all the traits than CC and CT genotype. Meanwhile for CLW, the additive effects were significant (P < 0.05). Hence, we suggest that genotype TT can be regarded as a potential molecular marker for later growth and carcass traits in chicken.

Association study of polymorphisms inside the miR-1657 seed region with chicken growth and meat traits

1. Polymorphisms occurring in the seed region of microRNAs (miRNAs) could influence their target gene and lead to phenotypic variation. The purpose of the research was to explore the genetic effects of the rs14934924 (G > A) mutation resident in the conserved seed region of miR-1657 on growth and meat traits of the Gushi-Anka F2 resource population. 2. The NdeI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and association analysis were used to analyse the polymorphism. 3. The mutation was associated with body weight at 8 weeks of age, shank girth at 12 weeks of age, breast bone length at 12 weeks of age, pelvis breadth at 4 weeks of age and subcutaneous fat thickness (P < 0·05) and was associated with body weight at 4, 6, 10 and 12 weeks of age (P < 0·01). 4. Our results will be a useful resource for a subsequent study in miRNA function, and provide a basis for molecular techniques in chicken breeding.

Growth Curves for Body Weight and Some Body Measurement of Ross 308 Broiler Chickens

Mendeş, M. 2009. Growth curves for body weight and some body measurement of Ross 308 broiler chickens. J. Appl. Anim. Res., 36: 85–88. To investigate the growth characteristics of Ross 308 male broiler chickens 30 male broiler chickens were used. Results of growth curve analyses suggest that the Gompertz growth model fitted the weight-age, shank width-age, shank length-age and body length-age data very well, while the linear growth model fitted the breast bone length-age, breast width-age and breast circumference-age data. The growth rate (b) for body weight (0.04) was found obviously smaller than that of shank width (0.23), shank length (0.18) and body length (0.23). Inflection point (k) for shank width and body length was found similar. For one weak increase in age a 19.49 mm enhancement in breast bone length, 6.41 mm enhancement in breast width and 4.06 cm enhancement in breast circumference are expected. All regression slope estimates (except for breast width) were significant.

Regression tree analysis for predicting slaughter weight in broilers

In this study, Regression Tree Analysis (RTA) was used to predict and to determine the most important variables in predicting the slaughter weight of Ross 308 broiler chickens. Data for this study came from 224 chickens raised during three different seasons, namely spring (n=66), summer (n=66), winter (n=92). Second week body weight, shank length, shank width, breast bone length, breast width, breast circumference and body length were used to predict the slaughter weight. Results of RTA showed that among the seven independent variables only four were selected, namely; body weight, breast bone length, shank width, and breast circumference. These selected independent variables were more efficient than the others in predicting the slaughter weight.RTA indicated that the birds which had values of second week body weight >295.95 g, breast bone length >55.82 mm and breast circumference >14.18 cm or that of body weight ≤295.95 g, breast bone length >60.26 mm and shank width >8.32 mm could be expected to have higher slaughter weights.