Abstract
ABSTRACT This study investigated SNP mutation sites of Gonadotrophin releasing hormone (GnRH) gene in China yellow quail, Beijing white quail and Korean quail through PCR amplification and DNA sequencing technologies. Moreover, polymorphism of GnRH gene and its association with growth traits of quail were analyzed, aiming to get molecular markers associated to growth traits of quail, which could provide references for breeding of new quail species. According to research results, a total of 14 SNP mutation sites of GnRH were detected in China yellow quail, Beijing white quail and Korean quail, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T and C458T. Except that only 2 genotypes were detected for A209C and C281T in China yellow quail and Beijing white quail, 3 genotypes were detected for all of the remaining 12 SNP mutation sites in three quail species. Of the 14 SNP sites, C71T, A209C, C215T, C281T, C293G, C339T and C458T were significantly associated with body weight (p<0.05), C71T, C108T, C168T, C178T, A184G, C206T, C215T, A252G, C293G, C339T and C458T were significantly associated with shank length (p<0.05), C71T, C215T, C293G and C458T were significantly associated with breastbone length (p<0.05), A209C and C281T were significantly associated with shank circumference (p<0.05).
Highlights
Gonadotrophin releasing hormone (GnRH) is a decapeptide firstly gained from hypothalamus of pig by Schally’s research team
Zhang et al (2019) discovered that GnRH mRNA was expressed in corpus leteum tissues of sow during different gestation periods, and the expression level was consistent with corpus leteum functions in the gestation period
The amplification fragments of GnRH gene all gave a high-definition bright stripe near 906bp, which conformed to the target size of 906bp
Summary
Gonadotrophin releasing hormone (GnRH) is a decapeptide firstly gained from hypothalamus of pig by Schally’s research team. G840327C of the GnRH-I gene and age at first egg (AFE) (p0.05) in a Chinese chicken population. The growth traits such as body weight, chest depth, chest width, breastbone length, body length, shank length and shank circumference were measured from quails at 7-17 weeks.
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