The stratigraphic architecture and environmental evolution of the Cerro Guido-Rio de las Chinas Valley Complex contains the upper Cretaceous to Eocene record of the Magallanes/Austral Basin, located in southernmost Chile. This retroarc foreland basin contains a significant record of early Cretaceous to Miocene biogeographic and environmental changes that occurred in high paleolatitudes after the break-up of Gondwana. Using 37 stratigraphic sections, we generated a 1390 m thick stratigraphic column, which was used to developed sedimentary facies and an analysis of sequence stratigraphy. Results show, in general, aggradation composed of six third-order depositional sequences each delimited by an erosive surface. A lowstand systems tract developed above each surface, represented by alluvial environments similar to braided fluvial systems, and coastal plain environments, with the development of meandering fluvial and lacustrine systems. Above the lowstand tract, the transgressive systems tract is composed of a shallow marine environment with foreshore, shoreface, and offshore systems. A highstand systems tract was observed in one sequence, and is represented by shallow marine facies. The extensive outcrop in the studied area contains a diverse and voluminous fossil record, containing fossilized invertebrates (bivalves, gastropods, bryozoans, and brachiopods), vertebrates (hadrosaurs, ornithischians, sauropods, theropods, birds, mammals, frogs, turtles, plesiosaurs, mosasaurs, and sharks), and plants (wood, leaves impressions, flowers, pollen, and spores). Thus, this study broadens the understanding of the South American-Antarctic connection during the late Cretaceous-Paleogene, through a diverse fossil record and study of the paleoenvironmental evolution of this high-latitude basin.