Brazilian Pantanal Research Articles

Invasive wild boar affected feral hog skull evolution over 25 years in Brazilian Pantanal wetland

Abstract The morphological plasticity of the Sus scrofa wild–domestic species complex is evident in both free-ranging morphotypes and domestic breeds. The Brazilian Pantanal feral hog (“porco-monteiro”) evolved after a long-term feralization process and represents a significant proportion of the mammalian biomass in the region. Its coexistence with native fauna brings several ecological impacts, which are mitigated by the Brazilian Pantanal wetland vastness and resource availability. They are a local subsistence game resource but also impact crops and pastures. Around the 2000s to mid-2010s, numerous European wild boar were introduced in Brazil to fulfill a demand for gourmet meat. Wild boar was also introduced to the Brazilian Pantanal as a game species and to breed with porco-monteiro feral hogs in a commercial venture to produce an even more exotic meat. The craze for wild boar meat has since dwindled, but their populations remain widespread and uncontrolled. Moreover, the full impact of the wild boar introduction on feral hog populations is still unknown. This study analyzes the skulls of porco-monteiro feral hogs from the 1990s, 2000s, and 2010s to assess possible morphological and functional variations along with skulls of wild boar and hybrids from Argentina, Uruguay, and Southern Brazil. The results indicate a trend of broadening of feral hog skulls at the coronal plane and a progressive reduction of the semispinalis capitis muscle scars over the decades. Biomechanical analysis denoted a decrease in head-elevation force that could affect the rooting performance of the latest feral hog morphotypes. Taken together, results show that wild boars likely influenced the evolution of the Pantanal feral hog skull. The magnitude of ecomorphological implications of these morphofunctional changes is unknown, as well as its effects in the Brazilian Pantanal wetland ecology.

Living Together, Singing Together: Revealing Similar Patterns of Vocal Activity in Two Tropical Songbirds Applying BirdNET.

In recent years, several automated and noninvasive methods for wildlife monitoring, such as passive acoustic monitoring (PAM), have emerged. PAM consists of the use of acoustic sensors followed by sound interpretation to obtain ecological information about certain species. One challenge associated with PAM is the generation of a significant amount of data, which often requires the use of machine learning tools for automated recognition. Here, we couple PAM with BirdNET, a free-to-use sound algorithm to assess, for the first time, the precision of BirdNET in predicting three tropical songbirds and to describe their patterns of vocal activity over a year in the Brazilian Pantanal. The precision of the BirdNET method was high for all three species (ranging from 72 to 84%). We were able to describe the vocal activity patterns of two of the species, the Buff-breasted Wren (Cantorchilus leucotis) and Thrush-like Wren (Campylorhynchus turdinus). Both species presented very similar vocal activity patterns during the day, with a maximum around sunrise, and throughout the year, with peak vocal activity occurring between April and June, when food availability for insectivorous species may be high. Further research should improve our knowledge regarding the ability of coupling PAM with BirdNET for monitoring a wider range of tropical species.

Detection of Brucella S19 Vaccine Strain DNA in Domestic and Wild Ungulates from Brazilian Pantanal.

The Pantanal region, the largest floodplain in the world, has a huge biodiversity and is an important livestock center. Bovine brucellosis has been reported in the region over the last three decades, posing implications for cattle industry as well as for the maintenance of biodiversity. We aimed to investigate the presence of B. abortus S19 vaccine strain DNA in unvaccinated domestic and wild ungulates from the Brazilian Pantanal. Fifty-two heifers, 63 ovine, 24 domestic pigs, 28 feral pigs, and three Pampas deer were sampled. Brucella spp. was detected through bcsp31 PCR of blood samples in 45.3% (77/170) of the sampled animals, of which 36.4% (28/77) showed positivity in ery PCR corresponding to B. abortus S19 strain. Feral pigs presented the highest occurrence of positive samples in bcsp31 PCR (75%), followed by ovine (47.6%), domestic pigs (41.7%), and unvaccinated heifers (30.8%). We did not observe positivity in Pampas deer. Our results strongly suggest that vaccination against bovine brucellosis may promote spill-over of B. abortus S19 strain in the Pantanal region. Moreover, our data indicate that wild strains of Brucella circulates in the Pantanal Biome.

Ichthyoplankton dynamics in the Brazilian Pantanal: Contribution of an important tributary and maintenance of connectivity

AbstractIn order to investigate the reproductive dynamics of the ichthyofauna in the Brazilian Pantanal, this study examines the spatial and temporal distribution of ichthyoplankton, taxonomic composition, and its correlation with environmental factors within one of the most important sub‐basins, the Taquari River. Egg and larvae collections were conducted at 11 sampling points between October and March of 2017/2018 and 2018/2019. Significant differences were observed between collection points, months, and years, with higher ichthyoplankton densities in the Coxim and Taquari rivers, particularly between November and January. Twenty‐eight genera and/or species of fish were identified, including 10 long‐distance migratory species. Egg density showed a negative correlation with water temperature and rainfall. For larvae, water transparency and rainfall were both significant, negatively influencing larval abundance. However, different species tend to occur in environments with distinct abiotic qualities. Yolk‐sac, preflexion, and flexion larval development stages were distributed throughout the sub‐basin, especially near the Pantanal. Considering the data and analyses presented in this study, the importance of connectivity between these environments, specifically between the plateau and Pantanal, is evident, highlighting the significance of the entire sub‐basin for fish reproductive processes. Therefore, research focusing on the ecological processes influencing the distribution of ichthyoplankton offers valuable insights for ecosystem conservation and aids in effective environmental management.

El legado matemático de la escritura cuneiforme: matemática funcional en la antigua mesopotamia

Tourism for the observation of large cats, such as the jaguar in the Brazilian Pantanal and the puma in Chilean Patagonia, has emerged as a powerful tool to promote biodiversity conservation and sustainable economic development. This article explores the opportunities and challenges associated with this form of tourism, highlighting best practices and strategies for maximizing benefits and mitigating negative impacts. The analysis reveals that big cat tourism can provide significant economic benefits to local communities, creating livelihoods and raising living standards. However, it is crucial to maintain a balance between economic benefits and ecological sustainability. Implementing capacity limits, strict regulations, and sustainable tourism practices are essential to prevent the degradation of natural habitats and animal stress. Tourism education plays a vital role in promoting responsible behaviors among visitors. Orientation programs and the presence of experienced guides help ensure that wildlife interactions are safe and informative. Additionally, collaborating with academic institutions to monitor and research the impact of tourism on feline populations and their habitats is critical for making informed decisions. The involvement of local communities is another critical aspect. Initiatives that ensure communities directly benefit from tourism strengthen local support for conservation efforts and create sustainable income streams. Habitat restoration projects and anti-poaching units, funded by tourism revenue, can promote ecological recovery and reduce threats to wildlife. Diversification of tourism activities is also recommended to distribute pressure on wildlife habitats and extend the stay of tourists. In addition to feline watching, cultural visits, ecological trails and other activities can promote conservation and environmental education. Jaguar and cougar sighting tourism presents a valuable opportunity to promote conservation and economic development. Adopting sustainable tourism practices, education, and community engagement are essential to ensure that this form of tourism continues to thrive, benefiting both wildlife and local communities.

Feline watching tourism in South America: Conservation, sustainability and impacts in the Pantanal and Patagonia

Tourism for the observation of large cats, such as the jaguar in the Brazilian Pantanal and the puma in Chilean Patagonia, has emerged as a powerful tool to promote biodiversity conservation and sustainable economic development. This article explores the opportunities and challenges associated with this form of tourism, highlighting best practices and strategies for maximizing benefits and mitigating negative impacts. The analysis reveals that big cat tourism can provide significant economic benefits to local communities, creating livelihoods and raising living standards. However, it is crucial to maintain a balance between economic benefits and ecological sustainability. Implementing capacity limits, strict regulations, and sustainable tourism practices are essential to prevent the degradation of natural habitats and animal stress. Tourism education plays a vital role in promoting responsible behaviors among visitors. Orientation programs and the presence of experienced guides help ensure that wildlife interactions are safe and informative. Additionally, collaborating with academic institutions to monitor and research the impact of tourism on feline populations and their habitats is critical for making informed decisions. The involvement of local communities is another critical aspect. Initiatives that ensure communities directly benefit from tourism strengthen local support for conservation efforts and create sustainable income streams. Habitat restoration projects and anti-poaching units, funded by tourism revenue, can promote ecological recovery and reduce threats to wildlife. Diversification of tourism activities is also recommended to distribute pressure on wildlife habitats and extend the stay of tourists. In addition to feline watching, cultural visits, ecological trails and other activities can promote conservation and environmental education. Jaguar and cougar sighting tourism presents a valuable opportunity to promote conservation and economic development. Adopting sustainable tourism practices, education, and community engagement are essential to ensure that this form of tourism continues to thrive, benefiting both wildlife and local communities.

Contributing to the knowledge of Pantanal Flora: a new endemic species of Dimerostemma (Asteraceae: Heliantheae) from Mato Grosso do Sul, Brazil

Dimerostemma pantanalense is a new species, which is described, illustrated, and discussed in this paper. The species can only be found in the municipalities of Ladário and Corumbá, Mato Grosso do Sul state, Pantanal, Brazil. It is preliminarily categorized as Critically Endangered (CR) due to the advance of urbanization and the risk of fires in the Pantanal. It is morphologically similar to D. annuum but differs by its life-cycle perennial (vs. annual), leaves opposite and alternate (vs. only opposite), sessile (vs. subsessile with petiole up to 5 mm long), lanceolate (vs. linear), venation suprabasal acrodromous imperfect (vs. hyphodromous), adaxial and abaxial leaf surfaces strongly villous to estrigose and glandular (vs. only nearly glabrous), receptacle convex to conical (vs. only convex), phyllaries 3-seriate (vs. 2-seriate), conduplicate palea carinate-alate (vs. not carinate-alate), cypselae oblanceolate to obovate (vs. subquadrangular) and pappus coroniform with two slightly developed awns (vs. coroniform with two well-developed awns). We present photographs and line drawings of the new species, along with discussions on its geographical distribution, habitat, phenology, and conservation status. Additionally, we provide an identification key for species of the genus in the Brazilian Pantanal.

Influence of Land Use and Land Cover on the Quality of Surface Waters and Natural Wetlands in the Miranda River Watershed, Brazilian Pantanal

Water quality assessment stands as a fundamental step in water resource management. In addition to substances and organisms present in the water, land use and land cover are two factors also affecting water quality. This study analyzed the correlation and influence between water quality and land use and land cover in the Miranda River Basin (MRB) and the natural wetlands in the Pantanal Biome. Using a watershed-wide approach, results suggested that water quality management policies can be considered in terms of sub-basins (smaller planning units). The Water Quality Index (WQI) was considered GOOD or EXCELLENT throughout the MRB. It was observed that the natural wetlands have the capacity to contribute to improving the WQI, with reductions in thermotolerant coliforms and turbidity; however, high values were found for thermotolerant coliforms, a temporal increasing trend of biochemical oxygen demand (BOD), and a reduction in dissolved oxygen and total phosphorus. Conversely, in these flooded areas, trends of a decrease in dissolved oxygen and an increase in BOD levels were found. Natural wetlands play important hydrological and ecological roles in water quality, storing, removing, and cycling nutrients. They are highly relevant areas for defining watershed management and conservation strategies, environmental protection, and providing ecosystem services.

Forest type modulates mammalian responses to megafires

Although considered an evolutionary force responsible for shaping ecosystems and biodiversity, fires’ natural cycle is being altered by human activities, increasing the odds of destructive megafire events. Here, we show that forest type modulates the responses of terrestrial mammals, from species to assemblage level, to a catastrophic megafire in the Brazilian Pantanal. We unraveled that mammalian richness was higher 1 year after fire passage compared to a pre-fire condition, which can be attributed to habitat modification caused by wildfires, attracting herbivores and open-area tolerant species. We observed changes in assemblage composition between burned/unburned sites, but no difference in mammalian richness or relative abundance. However, by partitioning the effects of burned area proportion per forest type (monospecific vs. polyspecific), we detected differential responses of mammals at several levels of organization, with pronounced declines in species richness and relative abundance in monospecific forests. Eighty-six percent of the species presented moderate to strong negative effects on their relative abundance, with an overall strong negative effect for the entire assemblage. Wildfires are predicted to be more frequent with climate and land use change, and if events analogous to Pantanal-2020 become recurrent, they might trigger regional beta diversity change, benefitting open-area tolerant species.

Essential and Non-Essential Elements Levels in Fish Species Highly Consumed in the Middle Miranda River, Brazilian Pantanal.

The present study investigated the levels of As, Ag, Al, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Se, and Zn in muscle samples of six fish species (Pygocentrus nattereri, Serrasalmus marginatus, Mylossoma duriventre, Leporinus friderici, Pimelodus argenteus, and P. pantaneiro) highly consumed by local riverine and non-resident people in middle Miranda River, southern Brazilian Pantanal. Significant differences were detected for Ba, Fe, Mn, and Zn levels among the analyzed species. Pairwise comparison detected significant differences in element levels between species with similar diets, which implies that other factors, instead of species' feeding habits, could be involved in distinct levels of element bioaccumulation. Significant correlations between body size and concentrations were found for Ba in P. nattereri (moderate positive correlation), and for Mn in M. duriventre (weak positive correlation) and P. argenteus (moderate negative correlation). Levels exceeding tolerable daily intake (TDI) were found for Pb maximum concentrations in P. nattereri, M. duriventre, L. friderici, P. argenteus, and P. pantaneiro, and for Cd maximum concentration in P. argenteus. Health risk assessment indicated considerable risk only for the worst-case scenario (calculated from maximum concentrations) for all species. Adopting a Pb monitoring program in biotic and abiotic compartments in Miranda River is strongly recommended.

Exploring Morphometric Features of the Female Reproductive Tract in Brazilian Pantanal Cattle

Exploring Morphometric Features of the Female Reproductive Tract in Brazilian Pantanal Cattle

Gastrointestinal Helminths in Wild Felids in the Cerrado and Pantanal: Zoonotic Bioindicators in Important Brazilian Biomes.

Environmental changes in the Brazilian Pantanal and Cerrado facilitate the spread of parasitic diseases in wildlife, with significant implications for public health owing to their zoonotic potential. This study aimed to examine the occurrence and diversity of gastrointestinal parasites in wild felids within these regions to assess their ecological and health impacts. We collected and analyzed helminth-positive samples from 27 wild felids using specific taxonomic keys. Diverse parasitic taxa were detected, including zoonotic helminths, such as Ancylostoma braziliense, Ancylostoma caninum, Ancylostoma pluridentatum, Toxocara cati, Toxocara canis, Dipylidium caninum, Taenia spp., Echinococcus spp., and Spirometra spp. Other nematodes, such as Physaloptera praeputialis and Physaloptera anomala, were identified, along with acanthocephalans from the genus Oncicola and a trematode, Neodiplostomum spp. (potentially the first record of this parasite in wild felids in the Americas). Human encroachment into natural habitats has profound effects on wild populations, influencing parasitic infection rates and patterns. This study underscores the importance of continuous monitoring and research on parasitic infections as a means of safeguarding both wildlife and human populations and highlights the role of wild felids as bioindicators of environmental health.

The Dynamics of Fire Activity in the Brazilian Pantanal: A Log-Gaussian Cox Process-Based Structural Decomposition

We present a novel statistical methodology for analyzing shifts in spatio-temporal fire occurrence patterns within the Brazilian Pantanal, utilizing remote sensing data. Our approach employs a Log-Gaussian Cox Process to model the spatiotemporal dynamics of fire occurrence, deconstructing the intensity function into components of trend, seasonality, cycle, covariates, and time-varying spatial effects components. The results indicate a negative correlation between rainfall and fire intensity, with lower precipitation associated with heightened fire intensity. Forest formations exhibit a positive effect on fire intensity, whereas agricultural land use shows no significant impact. Savannas and grasslands, typical fire-dependent ecosystems, demonstrate a positive relationship with fire intensity. Human-induced fires, often used for agricultural purposes, contribute to an increase in both fire frequency and intensity, particularly in grassland areas. Trend analysis reveals fluctuating fire activity over time, with notable peaks in 2018–2021.

Introduction dynamics: the advancement of peacock bass (Cichla sp.) in aquatic ecosystems of the pantanal (mato grosso)

The aim of this study was to survey the spatial distribution of the Cichla sp. species in aquatic bodies around the municipality of Cáceres-MT and the evolution of records for the Brazilian Pantanal. Collections took place in the marginal lakes and troughs of the Caramujo, Padre Ignácio and Paraguay rivers, with collections during high and low water periods in 2018 and 2019. Interviews were conducted with fishermen using a semi-structured questionnaire and obtaining locations where tucunaré occur. Secondary data was obtained by searching for articles on the occurrence of species in the Pantanal. Thirty-five specimens of Cichla kelberi were caught in 21 localities in the study area. Interviews revealed 44 localities, predominantly on the left banks of the Paraguay River. In the literature, there have been 9 studies on the occurrence of tucunaré in the Brazilian Pantanal, 7 of which in the southern Pantanal. The data points to a distribution of C. piquiti further south and C. kelberi further north in the biome, which may be due to the routes of introductions and the characteristics of the biome reducing the speed of diffusion.

Black soils in the southwest of the Brazilian Pantanal: Organic carbon and secondary carbonates accumulation in Phaeozems–Gleysol-Chernozem

The southwest of the Brazilian Pantanal contains areas formed by calcareous sediments, which give this hydromorphic environment special characteristics in the formation of soils such as Phaeozems, Chernozems and Gleysols. These soils have a dark surface horizon, enriched with organic matter, high natural fertility in the sorption complex and high clay activity. These soils are important because they store large amounts of organic carbon and can be used in carbon sequestration and greenhouse gases studies. However, despite their abundance in the Pantanal region and importance, these soils are few studied. Thus, our study aimed to characterize the morphology, physical, and chemical attributes of black soils in this region, identifying the main mechanisms of organic carbon and secondary carbonate accumulation. Three profiles located in the municipality of Corumbá-MS were studied, namely Phaeozem (profiles P1), Gleysol (profile P2) and Chernozem (profiles P3). In all profiles, high concentrations of Ca2+, Mg2+ and K+ elements were observed, mainly derived from the sediments of the Serra da Bodoquena and the Urucum-Amolar residual plateau, ion transport fluxes and secondary carbonate dissolution. The preservation of these elements and 2:1 clays is closely linked to the rainfall dynamics of the region, alternating between floods and droughts. The high 2:1 clays and exchangeable Ca2+ benefits the stabilization of organic carbon by means of organic–mineral interactions and promote the increase of humin content. In Phaeozem and Chernozem, the highest levels of secondary carbonates were observed at the top of the B horizons of these soils, where the pressure of CO2 is lower. Being a lowland area under the direct influence of flooding, the development of secondary carbonates is limited in the B horizons of the Gleysol, where access to the water table and root activity at depth increases the CO2 pressure and hinders the re-precipitation of carbonates in this soil, precipitating in the capillary bangs and in the upper zone (surface horizons).

Characteristics of extreme meteorological droughts over the Brazilian Pantanal throughout the 21st century

The Brazilian Pantanal is a remarkable ecosystem, encompassing vital aspects such as fish, fruit, and grain production, greenhouse gas emission regulation, temperature and precipitation modulation, and groundwater recharge. However, 2019 and 2020 experienced prolonged and severe drought that profoundly impacted the Pantanal region. Although these impacts are still under review, their effects on wildlife, vegetation, and the local population are undeniable. To enhance our understanding of the magnitude and consequences of the 2019/2020 drought, the present study conducted a comprehensive evaluation of drought occurrences in the Pantanal biome. Utilizing two well-established drought indices, the standard precipitation index (SPI) and the standard precipitation evapotranspiration index (SPEI), we analyzed the available observational data provided by the Climate Research Unit (CRU). Additionally, we endeavored to project droughts throughout the 21st century, thereby facilitating the visualization of potential reoccurrences of hazardous events in the Pantanal area. Two regional climate model (RCM) ensembles, the Eta ensemble (E1) and CORDEX ensemble (E2), along with representative concentration pathways (RCP4.5 and RCP8.5 scenarios) were used to project the severity of droughts in the Brazilian Pantanal until 2098/2099. The findings indicate that the hydrological year of 2019/2020 was the driest on record in the Pantanal over the past 80 years. The E1 ensemble did not project such an extreme event, whereas the E2 ensemble forecasted extreme droughts before the 2040s under the RCP4.5 and RCP8.5 scenarios. Using the SPEI, both ensembles showed similar results, with extreme droughts projected toward the end of the 21st century. Notably, the SPI demonstrated effectiveness in detecting short-term droughts, while the SPEI was more efficient for long-term assessments, projecting extreme drought events for the last two decades. The SPEI, employed with the Eta and CORDEX ensembles, can provide valuable insights into drought projections. Moreover, the potential recurrence of extreme droughts, characterized by significant below-average precipitation spanning vast areas throughout the hydrological year, poses a severe threat to the Pantanal biome in this century.

Cenas de um Brasil sem saída: imagens do neossujeito na telenovela Pantanal

The study addresses the Brazilian soap opera Pantanal (2022) from the perspective in which the narrative presents the character Madeleine. The reiteration of aesthetic arrangements made up of elements such as water and checkered domestic environments place the character in images of anesthetic landscapes, making it possible to infer the figurativeness of the neo-subject. Considering the analysis frames of the chapters shown between March 31, 2022 and May 21, 2022 – from the first appearance of the character until the moment of the plane crash that ends the girl’s trajectory in the plot –, it is possible to assume that the investigated scenes, in terms of the produced meaning effects, function as a metaphor and metonymy of a neoliberal apparatus, allowing the audience to perceive itself housed, eventually, in places similar to those that are framed by the telenovela and occupied by the character.

Relationship of the “Dequada” Phenomenon with Mercury Methylation in Pantanal, Brazil

The illegal activity of gold mining in the Brazilian Pantanal is contaminating the aquatic ecosystem with mercury. This contamination has been potentiated by mercury methylation due to a typical natural phenomenon of the Pantanal ecosystem known as “dequada”. The present study estimated in the laboratory the temporal production of methylmercury at two timepoints: the beginning of a flood, when the “dequada” phenomenon occurs, and the dry season without such phenomenon. After collecting water and sediment, experiments were performed with microcosms to reproduce conditions close to nature. To monitor the concentration of methylmercury, 1.0 mg L−1 of Hg2+ was added to water and sediment experiments in the Castelo and Saracura bays, both during the “dequada” and without it; monitoring lasted for 25 days. The methylmercury concentration was analyzed using atomic fluorescence spectrometry, total organic carbon using TOC 5000A, and physical and chemical parameters such as pH, redox potential, and dissolved oxygen with a multi-parameter probe. The results led to the conclusion that the “dequada” phenomenon significantly affected mercury methylation, because the environmental changes caused by this phenomenon such as the increased concentration of organic matter, decreased dissolved oxygen, and decreased pH, potentiated mercury methylation. During the “dequada” in the Castelo bay, the methylation rate was 3.5 times higher (6297.56 ng L−1) than in the experiment without the “dequada” (1827.44 ng L−1). Therefore, the gold mining activity in Pantanal can cause great damage to the ecosystem because this environment favors mercury methylation, making its most toxic form active in the aquatic ecosystem of Pantanal.

Indicator‐based natural capital reporting to inform decision‐making in the Brazilian Pantanal

Indicator‐based natural capital reporting to inform decision‐making in the Brazilian Pantanal

Effects of reproductive seasonality on the excretion of fecal glucocorticoid metabolites in free‐ranging Pampas Deer

AbstractUnderstanding how fecal glucocorticoid (GC) concentration is associated with reproduction in wild animals allows us to associate physiological stress with the costs of reproduction. Glucocorticoids are among the main stress‐related hormones, and their secretion is strongly associated with reproductive seasonality. Using non‐invasive methods (thereby avoiding causing stress), we used fecal GC metabolites (FGC) to test the hypothesis that the reproductive phase (mating, non‐mating, gestation, and lactation) influences stress levels of the seasonally reproductive Pampas Deer (Ozotoceros bezoarticus). Furthermore, we compared FGC between sexes and between males of different antler statuses (velvet, hard, cast). During 1 year, in the Pantanal of Brazil, we collected 621 fresh fecal samples (327 from females, 294 from males) from which we estimated FGC using enzyme immunoassay (EIA). FGC concentrations varied by reproductive phase and antler status. Agonistic and courtship interactions associated with mating (i.e., fights between males, attempts to mount females), suggest that they influence FGC concentrations most strongly in both sexes. Females also had greater FGC concentrations during lactation, suggesting that this phase and parental care are also associated with increased physiological stress. In males, the association of FGC concentrations with antler status may be, in part, associated with photoperiod and testosterone secretion, both of which can trigger reproductive and agonistic behaviors. Finally, reproductive synchrony of the sexes causes similar FGC over time and suggests that environmental factors contribute as well. We show that non‐invasive monitoring of glucocorticoid metabolites levels is an effective tool for detecting changes in the physiological stress response in Pampas Deer, suggesting that this tool will be useful for detecting changes in physiological stress caused by human disturbance, such as cattle ranching in Pantanal and similar disturbances elsewhere.