Brazilian Cerrado Biome Research Articles

Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome

Many soil microorganisms are able to transform insoluble forms of phosphorus to an accessible soluble form, contributing to plant nutrition as plant growth-promoting microorganisms (PGPM). The objective of this work was to isolate, screen and evaluate the phosphate solubilization activity of microorganisms in maize rhizosphere soil to manage soil microbial communities and to select potential microbial inoculants. Forty-five of the best isolates from 371 colonies were isolated from rhizosphere soil of maize grown in an oxisol of the Cerrado Biome with P deficiency. These microorganisms were selected based on the solubilization efficiency of inorganic and organic phosphate sources in a modified Pikovskaya's liquid medium culture containing sodium phytate (phytic acid), soybean lecithin, aluminum phosphate (AlPO 4), and tricalcium phosphate (Ca 3(PO 4) 2). The isolates were identified based on nucleotide sequence data from the 16S ribosomal DNA (rDNA) for bacteria and actinobacteria and internal transcribed spacer (ITS) rDNA for fungi. Bacteria produced the greatest solubilization in medium containing tricalcium phosphate. Strains B17 and B5, identified as Bacillus sp. and Burkholderia sp., respectively, were the most effective, mobilizing 67% and 58.5% of the total P (Ca 3(PO 4) 2) after 10 days, and were isolated from the rhizosphere of the P efficient L3 maize genotype, under P stress. The fungal population was the most effective in solubilizing P sources of aluminum, phytate, and lecithin. A greater diversity of P-solubilizing microorganisms was observed in the rhizosphere of the P efficient maize genotypes suggesting that the P efficiency in these cultivars may be related to the potential to enhance microbial interactions of P-solubilizing microorganisms.

LARVICIDAL ACTIVITY OF SOME CERRADO PLANT EXTRACTS AGAINST AEDES AEGYPTI

One hundred ninety hexanic and ethanolic extract from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 microg/ml. Fourteen extracts from 7 species showed activity (>65% mortality) against the larvae. Of these Dugeutia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 microg/ml, respectively. Annona crassiflora and Cybistax antisyphilitica showed activity at 23.06 and 27.61 microg/ml. The larvicidal properties of these species are described for the first time, and may prove to be promising in active chemical compound isolation.

Relationships between drosophilids (Diptera, Drosophilidae) and the environment in two contrasting tropical vegetations

Although natural populations of drosophilid flies have been the subject of ecological studies, the population ecology of these insects in the tropics is still poorly known. This paper discusses aspects of the relationship between drosophilids and their environment, based on 28 monthly collections made in two contrasting vegetations of the Brazilian Cerrado biome: gallery forest and savanna. Exotic species were found in both types of environment; but 14 of the 30 captured Neotropical species occurred exclusively in the gallery forests, probably because of their climatic stability and greater environmental heterogeneity. Even though some endemic species were more abundant in the dry and cold months, most populations exhibited peaks of abundance in the wet season. The species diversity indexes (H′ and D), higher in the dry season, were probably affected by increased evenness at this time of year, when the populations of practically all the species are greatly reduced. As species richness in the savanna vegetation clearly decreased in the dry season, increasing again in the wet season, it is suggested that some drosophilids migrate to the forests when climatic conditions are too stressful in the savannas. © 2006 The Linnean Society of London, Biological Journal of the Linnean Society, 2006, 87, 233–247.

Down-modulation of nitric oxide production in murine macrophages treated with crude plant extracts from the Brazilian Cerrado

Several plant species from the Cerrado biome in Brazil are popularly used as herbal medicines for its reputed analgesic, anti-acid, anti-microbial, anti-inflammatory and anti-tumoral properties, among others. It has been reported that some plant extracts interfere in the production of nitric oxide (NO), an important inflammatory mediator. In the present study, we investigated the effect of hexanic and ethanolic extracts from three plant species on NO production by LPS/IFN-γ-activated J774 macrophages based on traditional use. The cytotoxic effect of the crude extracts was determined by the thiazolyl blue test (MTT) to measure cell viability. Serjania lethalis stem extracts and Cupania vernalis leaf extracts significantly inhibited NO production, while extracts from Casearia sylvestris var. lingua were inactive or showed low activity on NO production, or were very cytotoxic. The ethanolic stem bark and leaf extracts of Serjania lethalis and Cupania vernalis, respectively, almost completely inhibited the production of NO by J774 macrophages. It can be concluded that the selected extracts are potential sources of active compounds that might be used as anti-inflammatory agents.

Biogeographic patterns, -diversity and dominance in the cerrado biome of Brazil

From a total woody flora of ca. 1000 species, a suite of 121 species forms an oligarchy dominating the cerrado biome. This mirrors patterns of dominance described in western Amazonian rain forests. Widespread sampling shows that across the biome this suite of species contributes on average 66% of the total species composition, and 75% of the total Importance Value Index in cerrado communities. An analysis of the floristic similarity between six cerrado phytogeographic provinces (southern, central and south-eastern, central-western, far-western, north-eastern, disjunct Amazonian) reveals great heterogeneity within the biome, principally of the less common species. Of the 951 species recorded from 375 floristic surveys across the biome, 494 species (more than half of the total) are found in only one of the provinces, with very few species (37, i.e., 3.9%) found in all six provinces. Each of the provinces contains a significant number of species which are apparently confined to it, ranging from 15 species (1.6% of the total woody cerrado flora) in the far-western province (primarily in the state of Rondonia) to 162 species (17%) in the central-western province. At the local level, floristic similarity can be very high. An analysis of the floristic composition of 13 sites within the Federal District shows a woody flora of 236 species, indicating that 25% of the total woody flora of the cerrado biome is represented in 0.3% of its area, illustrating the great conservation importance of this region. Floristic similarity between sites within the Federal District is high, although much of this similarity is accounted for by ‘oligarch’ species which account for between 59 and 89% of total species recorded per site. Informed conservation judgements within the cerrado need to take account of regional floristic patterns to ensure maximum protection of biodiversity, as the majority of species are not geographically widespread within the biome.

Seasonal landscape and spectral vegetation index dynamics in the Brazilian Cerrado: An analysis within the Large-Scale Biosphere–Atmosphere Experiment in Amazônia (LBA)

The Brazilian Cerrado biome comprises a vertically structured mosaic of grassland, shrubland, and woodland physiognomies with distinct phenology patterns. In this study, we investigated the utility of spectral vegetation indices in differentiating these physiognomies and in monitoring their seasonal dynamics. We obtained high spectral resolution reflectances, during the 2000 wet and dry seasons, over the major Cerrado types at Brasilia National Park (BNP) using the light aircraft-based Modland Quick Airborne Looks (MQUALS) package, consisting of a spectroradiometer and digital camera. Site-intensive biophysical and canopy structural measurements were made simultaneously at each of the Cerrado types including Cerrado grassland, shrub Cerrado, wooded Cerrado, Cerrado woodland, and gallery forest. We analyzed the spectral reflectance signatures, their first derivative analogs, and convolved spectral vegetation indices (VI) over all the Cerrado physiognomies. The high spectral resolution data were convolved to the MODIS, AVHRR, and ETM + bandpasses and converted to the normalized difference vegetation index (NDVI) and the enhanced vegetation index (EVI) to simulate their respective sensors. Dry and wet season comparisons of the measured biophysical attributes were made with the reflectance and VI data for the different Cerrado physiognomies. We found that three major domains of Cerrado could be distinguished with the dry and wet season spectral signatures and vegetation indices. The EVI showed a higher sensitivity to seasonality than the NDVI; however, both indices displayed seasonal variations that were approximately one-half that found with the measured landscape green cover dynamics. Inter-sensor comparisons of seasonal dynamics, based on spectral bandpass properties, revealed the ETM +-simulated VIs had the best seasonal discrimination capability, followed by MODIS and AVHRR. Differences between sensor bandpass-derived VI values, however, varied with Cerrado type and between dry and wet seasons, indicating the need for inter-sensor VI translation equations for effective multi-sensor applications.

Genetic studies in representatives of genusRhipidomys (Rodentia, Sigmodontinae) from Brazil

Karyotypic polymorphism of five taxa of the rodent genusRhipidomys from the Brazilian Amazon and Cerrado biomes was analysed.Rhipidomys nitela Thomas, 1901 from Amazon has 2n=48, FN=68. The other species, all have 2n=44, but can be separated into two groups, one with high FNs (76, 80) and the other with low FNs (48, 52). Two cytotypes ofR. mastacalis (Lund, 1840) with high FNs were trapped in four localities of the Cerrado, showing 19 and 17 biarmed autosomes, respectively. A low FN (48) was observed inR. leucodactylus (Tschudi, 1844) in two localities of the Cerrado and FN=52 in one locality in the Cerrado and the Amazon. All taxa with 2n=44 have a medium-sized acrocentric X chromosome and a small Y.Rhipidomys nitela is different from the species with 2n=44 by presenting a heterochromatic short arm of the X chromosome. In all karyotypes analysed, the nucleolus organizer regions were located in the short arms of two to six pairs and the (T2AG3)n telomeric probes hybridizedin situ in both the short and long arms of all pairs of the karyotypes.

Karyologic evidence of diversification of the genus Thrichomys (Rodentia, Echimyidae)

Karyologic analysis of Thrichomys specimens from different Brazilian localities, in Pantanal, Cerrado and Caatinga biomes, shows different chromosome complements. The 2n = 34, FN = 64 karyotype is found in Mato Grosso do Sul state; the 2n = 30, FN = 54 karyotype in Bahia, Pernambuco, Piauí and Ceará states; the 2n = 30, FN = 56 karyotype in Goiás and Tocantins states, the 2n = 28, FN = 50 karyotype in Minas Gerais state, the 2n = 28, FN = 52 and 2n = 26, FN = 48 karyotypes in Bahia state. Comparisons of G-band patterns allowed the identification of homologies shared by all karyotypes and show that the two karyotypes with the lowest diploid number (2n = 26 and 2n = 28) belong to two different evolutionary lineages. The most proper names for each karyomorphic population are: Thrichomys pachyurus for 2n = 34; Thrichomys apereoides apereoides for 2n = 28, FN = 50; Thrichomys apereoides laurentius for 2n = 30, FN = 54 and Thrichomys inermis for 2n = 26. Two karyotypes (2n = 28, FN = 52 and 2n = 30, FN = 56) could not be attributed to any subspecies. These different karyomorphotypes are allopatric and/or parapatric.

Loranthaceae e Viscaceae no Bioma Cerrado

The aim of this study to seek subsidies to define phytogeografic patterns in the Cerrado Biome of Brazil. The study was based on the botanic collections of the main herbaria from the Cerrado Biome. It consists of 2 chapters: l) Loranthaceae and Viscaceae synopsis representing the first studies of these families for the Cerrado Biome. Seventy-three species were found and 8 genera: Dendrophthora Eichl.; Phoradendron Nutt.; Oryctina Eichl.; Oryctanthus (Gris.) Eichl. ; Phthirusa Linn.; Psittacanthus Mart.; Struthanthus Mart.; Tripodanthus Van Tiegh.; 2) Phytogeography of the Loranthaceae / Viscaceae in the Cerrado Biome. The geographic coordinates were obtained from the 17 Brazilian herbarium and registered in an Excel table; maps were then produced by the Maptitude Geographic Information System. A similarity matrix was built using the Jaccard coefficient. The rectangles were clustered using UPGMA (Unweighted Pair Groups Method using Arithmetic Averages). This technique produced six provinces for the Cerrado Biome. Loranthaceae and Viscaceae showed patterns of geographic distribuition that vary from widely distributed species, in and out of the Cerrado Biome, to restricted endemics

The Brazilian Cerrado Vegetation and Threats to its Biodiversity

The Brazilian cerrado (savanna) biome covers 2 million km2representing 23% of the area of the country. It is an ancient biome with rich biodiversity, estimated at 160000 species of plants, fungi and animals. There are about 800 species of trees and large shrubs in the savanna vegetation and several times that number of ground species (herbs and subshrubs). When the flora of gallery forests, mesophytic forests and other habitats occurring in the biome are included, the total number of vascular plant species is estimated to reach about 10000. During the last 25 years modern agriculture has been developed in the cerrado to produce soya, maize, rice, etc and enormous numbers of cattle are raised in planted pastures. Charcoal production for the Brazilian steel industry also causes great destruction of the cerrado. By 1994 an estimated 695000 km2of cerrado (representing 35% of its area) had been converted to ‘anthropic landscape’. This compares to the destruction of about 400000 km2of Brazilian Amazonian forest representing 12 or 13% of the area of this biome. Conservation initiatives are now desperately needed. Only 1.5% of the cerrado biome is preserved as Federal Reserves and this area needs to be at least tripled. Surveys of the vascular flora aimed at discovering biogeographic patterns are now in progress with the objective of choosing representative areas and biodiversity ‘hot spots’ for conservation.