This work evaluated the production of cellulases and accessory enzymes by nine Trichoderma strains from the Brazilian Amazon Forest in comparison to the reference strain Trichoderma reesei RUT C-30. A selected T. harzianum strain showed total cellulase activity of 1.63 FPU/mL, which was higher than that measured for T. reesei RUT C-30. T. harzianum also produced high β-glucosidase levels, reaching 3.55 BGU/mL. The cellulolytic efficiency of T. harzianum and T. reesei Rut C-30 supernatants supplemented with Aspergillus awamori supernatant as a source of β-glucosidase and accessory enzymes were evaluated under comparative conditions using ball-milled and hydrothermally treated sugarcane bagasse. Both enzyme preparations showed comparable glucose yields of 72% for the low crystalline ball-milled material. However, T. harzianum enzymes were 25% less efficient than those of T. reesei-A. awamori for the hydrolysis of hydrothermally treated bagasse, which presents a higher crystallinity. Nevertheless, the association of a high cellulolytic activity and a high β-glucosidase activity in the same microorganism indicates the industrial potential of this new wild type T. harzianum strain.