Brassica Chinensis Research Articles

Zn Check: Revolutionizing Plant Nutrition with Amino Acid-based Zinc Chelation

Zinc plays a vital role in plant growth and development, impacting essential physiological processes such as cell division, nitrogen, carbohydrate metabolism and chloroplast development. However, zinc deficiency poses a significant challenge in agriculture, particularly in crops grown on calcareous soils. Traditional zinc fertilizers, like Zn EDTA, face limitations in alkaline soils, prompting the need for innovative solutions. This study introduces an amino acid-based chelating agent, Zinc Check, synthesized to address these challenges effectively. The research demonstrates the stability and efficacy of Zinc Check across diverse soil conditions, ensuring zinc availability to plants. Through various analytical techniques and pot trials with Pak choi plants, the study confirms the effectiveness of Zinc Check in promoting plant growth and chlorophyll synthesis, surpassing the performance of conventional zinc fertilizers. Moreover, the investigation explores the pH tolerance of Zinc Check, highlighting its stability across a wide pH range. Overall, this study underscores the importance of innovative solutions like Zinc Check in addressing zinc deficiency and enhancing agricultural productivity, contributing to global food security efforts.

Onion Peel Waste Has the Potential to Be Converted into a Useful Agricultural Product to Improve Vegetable Crop Growth

The onion processing industry produces hundreds of thousands of tons of onion waste annually. Normally, onion peel waste is dumped in landfills, which creates additional sources of greenhouse gases. Research has validated that onion peel is a concentrated source of bioactive compounds; therefore, it can be turned into useful agricultural products such as soil amendments and possibly biostimulants. This study conducted three experiments to investigate the plant growth-promoting potential of an onion juice concentrate (OJC). The first experiment explored whether the application of OJC could increase plant growth of Bermuda grass, lettuce, and bok choy. The second experiment evaluated the effects of foliar and subsurface drench applications of OJC on bok choy and lettuce growth. The third experiment investigated the interaction between OJC application methods and fertilizer type on bok choy and radish growth. The results indicated that foliar applications of OJC of 1% to 2% concentrations increased the yield of bok choy and its overall growth. Subirrigation with OJC, however, enhanced the root growth of bok choy, lettuce, and radish. Notably, the combined approach of foliar and subirrigation applications further promoted the growth of both bok choy and radish. Comparing across experiments, longer OJC application periods emerged as a promising strategy for amplifying its growth-promoting benefits. Overall, our findings suggest that OJC holds promise for promoting sustainable agriculture. This potential comes from its ability to enhance both the growth and yield of vegetable crops like bok choy, lettuce, and radish while simultaneously reducing waste.

Analysis of Glucosinolates and Phenolic Content in Sprouts of 7 Brassica rapa Subspecies

Objective: Brassica rapa is an important source of phytochemicals essential for plant defense and human health. To better understand their metabolic characteristics, this study aimed to investigate the glucosinolates and phenolic content from sprouts of 7 B. rapa subspecies. Methods: Glucosinolates and phenolic compounds were extracted using previously reported procedures, with slight modifications. Glucosinolates were identified based on their high-performance liquid chromatography peak area ratios and quantified based on the peak areas, retention time, and response factor and phenolic compounds were identified based on retention times and spiking tests and quantified concerning a corresponding calibration curve. Results: Statistical analysis showed that Choy sum (31.82 ± 2.3 mg/g DW) and Bok choy (31.73 ± 1.49 mg/g DW) presented the highest total glucosinolates content, and the lowest content was observed in Turnip rape (21.75 ± 1.67 mg/g DW). Between the contents of individual glucosinolates, progoitrin presented the highest content in Bok choy (17.84 ± 1.24 mg/g DW). The highest concentration of total phenolic compounds was found in the sprouts of Chinese cabbage (2.7 ± 0.05 mg/g DW), whereas the lowest concentration was found in Choy sum sprouts (2.17 ± 0.05 mg/g DW). The highest individual phenolic content was observed for benzoic acid (0.96 ± 0.03 mg/g DW) and rutin (0.85 ± 0.03 mg/g DW), which were highest in mizuna and Chinese cabbage, respectively. Conclusion: Subspecies may influence the levels of glucosinolates and polyphenols in vegetables. The results of our study showed the distinctive metabolic signatures embedded in the 7 B. rapa subspecies.

Application of majapahit (Crescentia cujete L.) fruit extract to control armyworm (Spodoptera litura Fabricius, 1775) infestation in Chinese mustard crops

Spodoptera litura poses a threat to important crops in Indonesia, leading to productivity failure and economic repercussions. Botanical insecticides have garnered attention in crop cultivation due to their easy applicability and effectiveness against specific pests. In this study, the effectiveness of majapahit (Crescentia cujete), a popular medicinal plant, in reducing S. litura infestation was investigated through in planta test using Chinese mustard (Brassica chinensis var. parachinensis). The experiment followed a completely randomized design (CRD) with four treatments of C. cujete fruit extract at concentrations of 0, 20, 40, 60, and 80%, each replicated four times. Parameters observed in this study included the growth profile of mustard plants (plant height, number of plants, fresh weight), leaf damage index (LDI), and mortality after application for 30 days after planting. Results revealed a significant concentration-response relationship for both the LDI values and mortality rates. The determination of LC50 for C. cujete fruit extract yielded a value of 29%. The use of C. cujete as an insecticidal agent warrants promotion, and further investigation with a larger sample population and different target crops is necessary in the future.

Improving The Growth and Yield of Pak Choy (Brassica chinensis L.) Using Cacao Pod Husk Biochar

This work aimed to assess the influence of cacao pod husk biochar treatments combined with the urea fertilizer addition on the growth and yield of pak choy (Brassica chinensis L.). The study used pot trials in a completely randomized design with two factors. The first was the addition of biochar from cacao pod husk, which consisted of 4 levels, namely 0, 62, 125, and 187 g/pot. The second factor was the dosage of urea addition with 4 levels, namely 0, 0.46, 0.93, and 1.40 g/pot. Pak choy was planted in triplicate polybags. Plant parameters included plant height, number of leaves, canopy cover area, fresh yield, water consumption, and water productivity. The results exhibited that adding biochar was significant in terms of parameters. Low dose biochar (62 g/pot) increased plant height, number of leaves, and water productivity, whereas high dose (125 to 187 g/pot) negatively affected pak choy growth and yield. The addition of urea is significant, except for pH and number of leaves. A fertilizer dose of up to 1.40 g/pot positively affected plant growth and yield of the pak choy. The most optimal interaction of the two factors occurred at a biochar dose of 62 g/pot and urea dose of 1.40 g/pot, which produced a maximum crop fresh yield of 111.83 g/plant. This research concluded that biochar from pyrolysis of cacao pod husk can be employed as soil amendment during pak choy cultivation but with controlled doses.

PENGARUH UMUR PINDAH TANAM BIBIT DAN APLIKASI BAKTERI FOTOSINTESA PADA SISTEM HIDROPONIK TERHADAP PERTUMBUHAN DAN HASIL PAKCOY (Brassica rapa L.)

The focus of the research was to find and analyze the interaction between the effect of transplanting age of seedlings and the application of photosynthetic bacteria on the growth and yield of pak choy (Brassica rapa L.). This research activity was carried out at the ARD Farm Garden from December 2023 to January 2024. Factorial experiment (4 x 3) with a total of 3 repetitions in the research. The first factor is the treatment of transplanting age (S), namely: S0: age 10 DAS, S1: age 13 DAS, S2: age 16 DAS. The second factor is the treatment of folier application of photosynthetic bacteria 2ml/liter (F), namely: F0: control application, F1: folier application 3 DAT, F2: folier application 7 DAT, F3: folier application 10 DAT. Analysis of variance (ANOVA) was used to analyze the data statistically before carrying out further tests using the Honestly Significant Difference (BNJ) at the 5% level. The research parameters observed included; number of leaves, leaf width, crown weight, and harvest index. The research results showed that the best growth of pak choy was in the transplanting treatment at 16 DAS with folier application at 7 DAT. The research results showed that the best pak choy harvest was in the transplanting treatment at 16 DAS and folier application at 10 DAT.

Effective remediation and phytotoxicity assessment of oxytetracycline and Cd co-contaminated soil using biochar

Heavy metal-antibiotic complex contamination in soil has raised much attention. Biochar is a common soil conditioner and pollutant barrier agent. However, there has rare research about biochar control effectiveness on heavy metals and antibiotics in plant-soil system. This work evaluated the impact of applying unmodified biochar (M) and H3PO4-modified biochar (PM) on Cd, OTC, and antibiotic resistance genes (ARGs) distribution in pakchoi (Brassica chinensis L.) and soil by pot experiment. Biochar can improve quality, antioxidative defense responses of plant and enzymes activity, physiochemical properties, microbial community of soil. The content of OTC, Cd, and ARGs in pakchoi were significantly reduced after applying biochar. There was a 73.08–78.48 % decrease rate in relative abundance of ARGs, a 58.96 %–59.66 % decrease rate in OTC content, and a 71.65 %–79.64 % decrease rate in available Cd content when biochar had been added to soil with OTC and Cd contaminations. The addition of 1.5 % biochar converted exchangeable Cd to residual fraction. Furthermore, applying PM biochar led to a greater reduction in Cd mobility and OTC in soil and their accumulation in pakchoi compared to M biochar. The biochar also mitigated the OTC and Cd phytotoxicity and improved plant quality and soil enzymes activity. Biochar could reduce Proteobacteria, Actinobacteria, Planctomycetes, Gemmatimonadetes, and Firmicutes abundancies. Soil organic matter accounted for the greatest proportion of variation in ARGs. Overall, the use of biochar, particularly PM biochar, was a promising approach for remediating soil contaminated with multiple pollutants.

Exploration the interaction of cadmium and copper toxic effects in pakchoi (Brassica chinensis L) roots through combinatorial transcriptomic and weighted gene co-expression network analysis

The interaction between cadmium(Cd) and copper(Cu) during combined pollution can lead to more complex toxic effects on humans and plants.However, there is still a lack of sufficient understanding regarding the types of interactions at the plant molecular level and the response strategies of plants to combined pollution. To assess this, we investigated the phenotypic and transcriptomic patterns of pakchoi (Brassica chinensis L) roots in response to individual and combined pollution of Cd and Cu. The results showed that compared to single addition, the translocation factor of heavy metals in roots significantly decreased (p < 0.05) under the combined addition, resulting in higher accumulation of Cd and Cu in the roots. Transcriptomic analysis of pakchoi roots revealed that compared to single pollution, there were 312 and 1926 differentially expressed genes (DEGs) specifically regulated in the Cd2Cu20 and Cd2Cu100 combined treatments, respectively. By comparing the expression of these DEGs among different treatments, we found that the combined pollution of Cd and Cu mainly affected the transcriptome of the roots in an antagonistic manner. Enrichment analysis indicated that pakchoi roots upregulated the expression of genes involved in glucosetransferase activity, phospholipid homeostasis, proton transport, and the biosynthesis of phenylpropanoids and flavonoids to resist Cd and Cu combined pollution. Using weighted gene co-expression network analysis (WGCNA), we identified hub genes related to the accumulation of Cd and Cu in the roots, which mainly belonged to the LBD, thaumatin-like protein, ERF, MYB, WRKY, and TCP transcription factor families. This may reflect a transcription factor-driven trade-off strategy between heavy metal accumulation and growth in pakchoi roots. Additionally, compared to single metal pollution, the expression of genes related to Nramp, cation/H+ antiporters, and some belonging to the ABC transporter family in the pakchoi roots was significantly upregulated under combined pollution. This could lead to increased accumulation of Cd and Cu in the roots. These findings provide new insights into the interactions and toxic mechanisms of multiple metal combined pollution at the molecular level in plants.

RAFT: An IoT-Based Nutrition Monitoring System for Bok Choy Hydroponics Plants

The Internet of Things (IoT) plays a crucial role in technology advancements, especially in the agricultural sector, such as hydroponics. Manual monitoring of parameters like nutrient levels, pH, and water levels in plants consumes farmers' time and energy and increases the risk of crop failure. This research aims to evaluate the effectiveness of using IoT RAFT (Remote Automated Farming Technology) system for farmers, particularly hydroponic bok choy farmers, in monitoring and controlling plant nutrient levels and the development process using waterfall as a research methodology. Parameters tested in this research include bok choy height, number of leaves, and bok choy harvest weight. We conducted this research on 14 plants for one harvesting period, then we used linear regression to determine the growth rate by calculating the slope. The results show that the plant height, the number of leaves, and harvest weight using the IoT RAFT system are 0.5897 cm/day, 0.6391 leaves/day, and 216.43 grams, respectively. We also compared the IoT RAFT system with a non-IoT bok choy growing method, and we concluded our IoT RAFT system has a better growth rate compared to the non-IoT bok choy growing method.

Differentiation of clubroot disease suppression in Pak choi under different concentrations of Trichoderma harzianum and fluazinam

Differentiation of clubroot disease suppression in Pak choi under different concentrations of Trichoderma harzianum and fluazinam

In-Depth Characterization of bZIP Genes in the Context of Endoplasmic Reticulum (ER) Stress in Brassica campestris ssp. chinensis.

Numerous studies have been conducted to investigate the genomic characterization of bZIP genes and their involvement in the cellular response to endoplasmic reticulum (ER) stress. These studies have provided valuable insights into the coordinated cellular response to ER stress, which is mediated by bZIP transcription factors (TFs). However, a comprehensive and systematic investigations regarding the role of bZIP genes and their involvement in ER stress response in pak choi is currently lacking in the existing literature. To address this knowledge gap, the current study was initiated to elucidate the genomic characteristics of bZIP genes, gain insight into their expression patterns during ER stress in pak choi, and investigate the protein-to-protein interaction of bZIP genes with the ER chaperone BiP. In total, 112 members of the BcbZIP genes were identified through a comprehensive genome-wide analysis. Based on an analysis of sequence similarity, gene structure, conserved domains, and responsive motifs, the identified BcbZIP genes were categorized into 10 distinct subfamilies through phylogenetic analysis. Chromosomal location and duplication events provided insight into their genomic context and evolutionary history. Divergence analysis estimated their evolutionary history with a predicted divergence time ranging from 0.73 to 80.71 million years ago (MYA). Promoter regions of the BcbZIP genes were discovered to exhibit a wide variety of cis-elements, including light, hormone, and stress-responsive elements. GO enrichment analysis further confirmed their roles in the ER unfolded protein response (UPR), while co-expression network analysis showed a strong relationship of BcbZIP genes with ER-stress-responsive genes. Moreover, gene expression profiles and protein-protein interaction with ER chaperone BiP further confirmed their roles and capacity to respond to ER stress in pak choi.

Evidence on the inhibitory effect of Brassica plants against Acinetobacter baumannii lipases: phytochemical analysis, in vitro, and molecular docking studies

BackgroundInfections caused by Acinetobacter baumannii are becoming a rising public health problem due to its high degree of acquired and intrinsic resistance mechanisms. Bacterial lipases penetrate and damage host tissues, resulting in multiple infections. Because there are very few effective inhibitors of bacterial lipases, new alternatives for treating A. baumannii infections are urgently needed. In recent years, Brassica vegetables have received a lot of attention since their phytochemical compounds have been directly linked to diverse antimicrobial actions by inhibiting the growth of various Gram-positive and Gram-negative bacteria, yeast, and fungi. Despite their longstanding antibacterial history, there is currently a lack of scientific evidence to support their role in the management of infections caused by the nosocomial bacterium, A. baumannii. This study aimed to address this gap in knowledge by examining the antibacterial and lipase inhibitory effects of six commonly consumed Brassica greens, Chinese cabbage (CC), curly and Tuscan kale (CK and TK), red and green Pak choi (RP and GP), and Brussels sprouts (BR), against A. baumannii in relation to their chemical profiles.MethodsThe secondary metabolites of the six extracts were identified using LC-QTOF-MS/MS analysis, and they were subsequently correlated with the lipase inhibitory activity using multivariate data analysis and molecular docking.ResultsIn total, 99 metabolites from various chemical classes were identified in the extracts. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) revealed the chemical similarities and variabilities among the specimens, with glucosinolates and phenolic compounds being the major metabolites. RP and GP showed the highest antibacterial activity against A. baumannii, followed by CK. Additionally, four species showed a significant effect on the bacterial growth curves and demonstrated relevant inhibition of A. baumannii lipolytic activity. CK showed the greatest inhibition (26%), followed by RP (21%), GP (21%), and TK (15%). Orthogonal partial least squares-discriminant analysis (OPLS-DA) pinpointed 9 metabolites positively correlated with the observed bioactivities. Further, the biomarkers displayed good binding affinities towards lipase active sites ranging from −70.61 to −30.91 kcal/mol, compared to orlistat.ConclusionThis study emphasizes the significance of Brassica vegetables as a novel natural source of potential inhibitors of lipase from A. baumannii.

Synergistic improvement of humus formation in compost residue by fenton-like and effective microorganism composite agents

Improving the humification of compost through a synergistic approach of biotic and abiotic methods is of great significance. This study employed a composite reagent, comprising Fenton-like agents and effective microorganisms (EM) to improve humification. This composite reagent increased humic-acid production by 37.44 %, reaching 39.82 g kg−1, surpassing the control group. The composite reagent synergistically promoted micromolecular fulvic acid and large humic acid production. Collaborative mechanism suggests that Fenton-like agents contributed to bulk residue decomposition and stimulated the evolution of microbial communities, whereas EMs promoted highly aromatic substance synthesis and adjusted the microbial community structure. Sequencing analysis indicates the Fenton-like agent initiated compost decomposition by Firmicutes, and EM reduced the abundance of Virgibacillus, Lentibacillus, and Alcanivorax. Applied as an organic fertilizer in Brassica chinensis L. plantations, the composite reagent considerably improved growth and photosynthetic pigment content. This composite reagent with biotic and abiotic components provides a learnable method for promoting humification.

Physiological and Transcriptomic Responses of Bok Choy to Heat Stress.

High temperatures have adverse effects on the yield and quality of vegetables. Bok choy, a popular vegetable, shows varying resistance to heat. However, the mechanism underlying the thermotolerance of bok choy remains unclear. In this study, 26 bok choy varieties were identified in screening as being heat-resistant at the seedling stage; at 43 °C, it was possible to observe obvious heat damage in different bok choy varieties. The physiological and biochemical reactions of a heat-tolerant cultivar, Jinmei (J7), and a heat-sensitive cultivar, Sanyueman (S16), were analyzed in terms of the growth index, peroxide, and photosynthetic parameters. The results show that Jinmei has lower relative conductivity, lower peroxide content, and higher total antioxidant capacity after heat stress. We performed transcriptome analysis of the two bok choy varieties under heat stress and normal temperatures. Under heat stress, some key genes involved in sulfur metabolism, glutathione metabolism, and the ribosome pathway were found to be significantly upregulated in the heat-tolerant cultivar. The key genes of each pathway were screened according to their fold-change values. In terms of sulfur metabolism, genes related to protease activity were significantly upregulated. Glutathione synthetase (GSH2) in the glutathione metabolism pathway and the L3e, L23, and S19 genes in the ribosomal pathway were significantly upregulated in heat-stressed cultivars. These results suggest that the total antioxidant capacity and heat injury repair capacity are higher in Jinmei than in the heat-sensitive variety, which might be related to the specific upregulation of genes in certain metabolic pathways after heat stress.

Assessing Seasonal Effects on Identification of Cultivation Methods of Short-Growth Cycle Brassica chinensis L. Using IRMS and NIRS.

Seasonal (temporal) variations can influence the δ13C, δ2H, δ18O, and δ15N values and nutrient composition of organic (ORG), green (GRE), and conventional (CON) vegetables with a short growth cycle. Stable isotope ratio mass spectrometry (IRMS) and near-infrared spectroscopy (NIRS) combined with the partial least squares-discriminant analysis (PLS-DA) method were used to investigate seasonal effects on the identification of ORG, GRE, and CON Brassica chinensis L. samples (BCs). The results showed that δ15N values had significant differences among the three cultivation methods and that δ13C, δ2H, and δ18O values were significantly higher in winter and spring and lower in summer. The NIR spectra were relatively clustered across seasons. Neither IRMS-PLS-DA nor NIRS-PLS-DA could effectively identify all BC cultivation methods due to seasonal effects, while IRMS-NIRS-PLS-DA combined with Norris smoothing and derivative pretreatment had better predictive abilities, with an 89.80% accuracy for ORG and BCs, 88.89% for ORG and GRE BCs, and 75.00% for GRE and CON BCs. The IRMS-NIRS-PLS-DA provided an effective and robust method to identify BC cultivation methods, integrating multi-seasonal differences.

Decoding early stress signaling waves in living plants using nanosensor multiplexing.

Increased exposure to environmental stresses due to climate change have adversely affected plant growth and productivity. Upon stress, plants activate a signaling cascade, involving multiple molecules like H2O2, and plant hormones such as salicylic acid (SA) leading to resistance or stress adaptation. However, the temporal ordering and composition of the resulting cascade remains largely unknown. In this study we developed a nanosensor for SA and multiplexed it with H2O2 nanosensor for simultaneous monitoring of stress-induced H2O2 and SA signals when Brassica rapa subsp. Chinensis (Pak choi) plants were subjected to distinct stress treatments, namely light, heat, pathogen stress and mechanical wounding. Nanosensors reported distinct dynamics and temporal wave characteristics of H2O2 and SA generation for each stress. Based on these temporal insights, we have formulated a biochemical kinetic model that suggests the early H2O2 waveform encodes information specific to each stress type. These results demonstrate that sensor multiplexing can reveal stress signaling mechanisms in plants, aiding in developing climate-resilient crops and pre-symptomatic stress diagnoses.

Effects of wollastonite and phosphate treatments on cadmium bioaccessibility in pak choi (Brassica rapa L. ssp. chinensis) grown in contaminated soils.

Cadmium (Cd) contamination of soil can strongly impact human health through the food chain due to uptake by crop plants. Inorganic immobilizing agents such as silicates and phosphates have been shown to effectively reduce Cd transfer from the soil to cereal crops. However, the effects of such agents on total Cd and its bioaccessibility in leafy vegetables are not yet known. Pak choi (Brassica rapa L. ssp. chinensis) was here selected as a representative leafy vegetable to be tested in pots to reveal the effects of silicate-phosphate amendments on soil Cd chemical fractions, total plant Cd levels, and plant bioaccessibility. The collected Cd contaminated soil was mixed with control soil at 1:0, 1:1, 1:4, 0:1 with a view to Cd high/moderate/mild/control soil samples. Three heavy metal-immobilizing agents: wollastonite (W), potassium tripolyphosphate (KTPP), and sodium hexametaphosphate (SHMP) were added to the soil in order to get four different treatment groups, i.e., control (CK), application of wollastonite alone (W), wollastonite co-applied with KTPP (WKTPP), application of wollastonite co-applied with SHMP (WSHMP) for remediation of soils with different levels of Cd contamination. All three treatments increased the effective bio-Cd concentration in the soils with varying levels of contamination, except for W under moderate and heavy Cd contamination. The total Cd concentration in pak choi plants grown in mildly Cd-contaminated soil was elevated by 86.2% after WKTPP treatment compared to the control treatment could function as a phytoremediation aid for mildly Cd-contaminated soil. Using an in vitro digestion method (physiologically based extraction test) combined with transmission electron microscopy, silicate and phosphorus agents were found to reduce the bioaccessibility of Cd in pak choi by up to 66.13% with WSHMP treatment. Application of silicate alone reduced soil bio-Cd concentration through the formation of insoluble complexes and silanol groups with Cd, but the addition of phosphate may have facilitated Cd translocation into pak choi by first co-precipitating with Ca in wollastonite while simultaneously altering soil pH. Meanwhile, wollastonite and phosphate treatments may cause Cd to be firmly enclosed in the cell wall in an insoluble form, reducing its translocation to edible parts and decreasing the bioaccessibility of Cd in pak choi. This study contributes to the mitigation of Cd bioaccessibility in pak choi by reducing soil Cd concentration through in situ remediation and will help us to extend the effects of wollastonite and phosphate on Cd bioaccessibility to other common vegetables. Therefore, this study thus reveals effective strategies for the remediation of soil Cd and the reduction of Cd bioaccessibility in crops based on two indicators: total Cd and Cd bioaccessibility. Our findings contribute to the development of methods for safer cultivation of commonly consumed leafy vegetables and for soil remediation.

Early detection of infestation by mustard aphid, vegetable thrips and two-spotted spider mite in bok choy with deep neural network (DNN) classification model using hyperspectral imaging data

Although exclusion measures (e.g., air filters, biosecurity practices) can be employed to prevent occurrence of pest outbreaks, indoors vegetable farms in Singapore are still susceptible to various arthropod pests. Due to strong interest from the industry to pursue pesticide-free production, indoors pest management is often focused on early detection for timely containment and eradication, implying the importance of robust and vigorous pest monitoring programs. In recent years, application of machine vision technologies, especially hyperspectral imaging (HSI), has been studied for their capacity to early detect pest infestation. However, there is a lack of studies conducted in actual indoor environments and on multiple arthropod pests. Thus, this study aimed to non-destructively collect hyperspectral data of bok choy Brassica rapa subspecies chinensis which were healthy or infested with either mustard aphids Lipaphis erisymi, vegetable thrips Echinothrips americanus or two-spotted spider mites Tetranichus urticae in indoor environment to build deep neural network (DNN) classification model for early detection. Based on HSI data of control and infested plants collected daily over a period of two weeks, we found that point percentage change (PPC) values associated with leaf reflectance in 420–440 nm, 500–520 nm, 620–637 nm, 720–800 nm, and 850 nm were sensitive to infestation by the mentioned arthropod pests. Deep Neural Network (DNN) classification models trained on collected HSI data were found to outperform Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM) classification models. DNN models achieved 92.8 ± 0.4 % overall classification accuracy across all days. As early as two days after infestation, DNN models could achieved classification precision values of 96.4 %, 96.9 %, 93.9 % and 100 % for control plants and plants infested with either aphids, spider mites or thrips respectively. These results highlight the feasibility of multiclass early detection of different arthropod pests and the potential of HSI system coupled with DNN classification as an autonomous plant health monitoring tool in indoor crop production.

Pengaruh Konsentrasi Beauveria Bassiana Terhadap Intensitas Serangan Serangga Hama Ulat Daun (Plutella xylostella L.), Pertumbuhan Dan Hasil Beberapa Varietas Pakcoy (Brassica rapa L.)

This research aims to determine the interaction between administering concentrations of B. bassiana and several varieties on attacks by leaf caterpillar insect pests in increasing plant growth, plant fresh weight and quality of pakcoy plants. The experiment was carried out on experimental land in Cibedug Village, Cikole Village, Lembang District, West Bandung Regency with an altitude of 1100 m above sea level (asl) with Andisol soil order and was carried out from August 2023 to September 2023. The experimental design used was an environmental design experiment using a Split Plot Design consisting of 12 treatment combinations repeated 3 times. consists of two treatment factors, namely B. bassiana concentration (K) with the following levels: k0 : 0 g L-1 Solution (control), k1 : 2 g L-1 Solution, k2 : 4 g L-1 Solution and k3 : 6 g L-1 Solution and the second factor are several Pakcoy varieties (V) with the following levels: v1: Nauli F1 variety, v2: Flaminggo variety, v3: Masbro variety, which is repeated three times. The results of the research showed that there was no interaction between the concentration of B. bassiana and several pak choy varieties on all observed parameters. Giving a B.bassiana concentration of 4 g L-1 Solution had the most efficient effect on the intensity of attacks, weight and length of the 3rd instar larvae of P.xylostella. The Nauli F1 variety gave the best results in terms of plant height and number of leaves and the Flaminggo variety gave the best results in terms of fresh weight per plant and fresh weight per plot.

Pemberdayaan Masyarakat Dalam Upaya Mandiri Pangan Melaui Hidroponik

People's need for food, such as fruit and vegetables, is increasing every year along with population growth. Efforts currently being made to meet the self-sufficient needs of the community's food are by importing, because local products have not been able to meet the food needs of the community due to the not yet optimal level of land productivity in Indonesia so that the hydroponic system is the most appropriate to address food problems because hydroponics is a cultivation system with take advantage of narrow land. The hydroponic training aims to support the introduction of more efficient and sustainable farming methods in the context of limited land and resources and then provide an understanding of farming using the hydroponic method for the people of Candi Baru RT 06/04, Genting village, Boyolali district. The MMK KKN Hydroponic training activities (special mission independent) were held on July 31, 2023. It was held in Dukuh Candi Baru Village, RT 06/04, Kec. Genting district. Boyolali coincided with a local resident's house. The hydroponic technique taught is a simple technique, namely thewick system, plant seeds taught in the hydroponic technique, namely kale, spinach, and bok choy. The results of the questionnaire recap show that the knowledge level of the participants has increased, this is shown by the graph which shows the level of knowledge after the training has increased above 80%. This shows the success of the training carried out by the Mandiri KKN Special Mission UIN Walisongo Semarang for the participants.