Brassica Chinensis Research Articles

Combined Biochar and Bentonite Application for Immobilization of Cadmium and Brassica Chinensis L. Growth in Contaminated Soil

Combined Biochar and Bentonite Application for Immobilization of Cadmium and Brassica Chinensis L. Growth in Contaminated Soil

Application of exogenous jasmonic acid enhances the resistance of pak choi (Brassica chinensis L.) to high levels of selenium-toxicity in the soil

Application of exogenous jasmonic acid enhances the resistance of pak choi (Brassica chinensis L.) to high levels of selenium-toxicity in the soil

Transcriptome and WGCNA Analyses Reveal Key Genes Regulating Anthocyanin Biosynthesis in Purple Sprout of Pak Choi (Brassica rapa L. ssp. chinensis).

Chinese cabbage is rich in vitamins, fibre, and nutrients and is one of the primary vegetables consumed in autumn and winter in South Asia. 'Purple pak choi' sprouts are particularly rich in anthocyanins and are favoured by consumers. However, reports on the regulation of anthocyanin synthesis in purple pak choi sprouts do not exist. In this study, we examined the phenotypic development of purple pak choi sprouts after germination. The total anthocyanin content increased from 0.02 to 0.52 mg/g FW from days 0 to 6. RNA-seq data analysis revealed an increase in differentially expressed genes corresponding to the development of purple pak choi sprouts. Expression pattern analysis of genes associated with the anthocyanin biosynthesis pathway revealed a significant upregulation of structural genes during the purple phase, suggesting that the transcription factors PAP2 and MYBL2 may play crucial regulatory roles. BraPAP2.A03, BraTT8.A09, and BraMYBL2.A07 exhibited strong interactions with key genes in the anthocyanin biosynthesis pathway, specifically BraDFR.A09. Furthermore, the expression of BraPAP2.A03 aligned with the expression patterns of most anthocyanin biosynthesis-related genes, whereas those of BraTT8.A09 and BraMYBL2.A07 corresponded with the expression pattern of BraDFR.A09. These results provide valuable insights into regulatory mechanisms underlying anthocyanin synthesis in purple pak choi sprouts.

Consumer liking of steamed green Chinese cabbage (Brassica rapa ssp. chinensis) cultivated in formulated vegetable waste-based organic hydroponic solutions

Chinese cabbage or pak choy (Brassica rapa ssp. chinensis) is often consumed because of its high nutritional content and several health advantages. Due to their promotion as being healthier and more ecologically friendly, hydroponic vegetables have seen a recent spike in consumer interest. The production of Chinese cabbage involved the processing of the vegetable waste to create various hydroponic solution formulations (F1 = banana peels, eggshells, and bean sprouts; F2 = banana humps, onion peels, bean sprouts, and moringa leaves; F3 = moringa leaves, onion peels, and bean sprouts; and F4 = AB Mix solution). They were applied at concentrations of 600, 900, 1,200, and 1,500 parts per million. The aim of this study was to investigate the relationship between the type of hydroponic growth solutions and Chinese cabbage customer acceptance. As a result, using a hedonic test of consumer liking, a panel of 32 untrained individuals was asked to show their liking score on sensory attributes of Chinese cabbage. There was a significant difference in the color liking of Chinese cabbage as an effect of hydroponic solution formulation. However, both organic vegetable waste-based formulations did not statistically substantially affect consumer acceptance, which includes color, taste, flavor, texture, and overall liking. Ultimately, vegetable waste-based organic formulations can be used to produce Chinese cabbage that is as acceptable to customers as those prepared with inorganic AB-Mix solution.

Respon Pertumbuhan Tanaman Pakcoy (Brassica rapa L.) Varietas Nauli F1 Melalui Pemberian Pupuk Kandang Sapi dan NPK 16-16-16

Pakcoy (Brassica rapa L.) is a type of vegetable plant that belongs to the Brassicaceae family. Pakcoy plants originate from China and have been cultivated since the 5th century widely in southern China and central China and Taiwan. The purpose of this study was to determine the response of growth and yield of pakcoy mustard plants (Brassica rapa L.) through the provision of organic fertilizer Cow Manure and Inorganic fertilizer NPK 16-16-16. This study used a Randomized Block Design (RAK), consisting of 2 factors and 4 levels with 3 Replications. Where the first factor is Cow Manure (P) with 4 levels, namely = P0 Control P1: (1 kg/plot), P2: (2 kg/plot), P3: (3 kg/plot). And the second factor is NPK 16-16-16 fertilizer with 4 levels, namely: N0: control, N1: (25 grams/plot), N2: (50 grams/plot), N3: (75 grams/plot). The parameters observed are: plant height (cm), number of leaves (strands). Analysis of variance with Analysis of variance (Anova), continued with DMRT test at 0.05% level if there is a real treatment. The average height of pak choy plants that gave the highest value was in treatment P2 = 19.15, N0 = 17.72 In the 28th observation hst, the number of leaves in treatment P1 = 114.21, N2 = 12.49. Keywords: pakcoy, cow manure, NPK 16-16-16.

Biogas Residue Carbonization Rather Than Biogas Residue Promoted the Yield of Pakchoi and Reduced the N2O Production Potential in Horticultural Soil

ABSTRACTBiogas residue (BR) and biogas residue‐derived biochar (BRC) are widely used as substitute for inorganic Nitrogen (N) fertilizers in vegetable production. Yet, their comparative research on vegetable production and N2O production was still lacking. Here, the pot experiment of pakchoi (Brassica chinensis) with a gradient of BR or BRC application rates (0, 30%, 60%, 100% w/w) was carried out to simulate different N fertilizer substitution rates. The results showed that the pakchoi yield had no difference between BR or BRC and control treatments; BRC had more advantages than BR in maintaining or increasing the pakchoi yield. BR and BRC could all reduce N2O production potential in vegetable soils. But BRC had a stronger ability to inhibit denitrification while BR had a stronger ability to inhibit nitrification compared with each other. The results showed that BR and BRC had different regulatory pathways for pakchoi yield and N2O production. BR regulated the pakchoi yield majorly through nitrification, but BRC majorly through denitrification. It suggested that BR and BRC could partially or completely replace inorganic fertilizers without reducing pakchoi yield. BRC combined with chemical fertilizers was a higher intelligence strategy in vegetable systems to improve pakchoi yield and N2O production compared with BR. It provided a theoretical basis for the application of BR and BRC to nutrient cycling and microbial processes in the soil‐vegetable system.

Effect of Paenibacillus favisporus CHP14 inoculation on selenium accumulation and tolerance of Pakchoi (Brassica chinensis L.) under exogenous selenite treatments

The effects of Paenibacillus favisporus CHP14 inoculation on selenium (Se) accumulation and Se tolerance of Pakchoi were studied by a pot experiment conducted in greenhouse. The results revealed that the growth traits such as plant height, root length, and biomass were significantly elevated during CHP14 treatment at 0 ∼ 8.0 mg·kg−1 Se(IV) levels. CHP14-inoculated plants accumulated more Se in root and shoot, which were 24.1%∼57.3% and 7.5%∼50.9% higher than those of non-inoculated plants. The contents of leaf nitrogen (N), phosphorus (P), magnesium (Mg), and iron (Fe), as well as the ratio of indoleacetic acid and abscisic acid contents (IAA/ABA) were increased by CHP14 inoculation, and positively associated with photosynthetic pigment contents (p < 0.05). At ≥ 4.0 mg·kg−1 Se(IV) levels, superoxide dismutase, peroxidase, and glutathione peroxidase activities of Pakchoi roots were increased with CHP14 inoculation, by 9.9%∼17.1%, 28.4%∼40.7%, and 7.4%∼15.3%, respectively. Moreover, CHP14 inoculation enhanced ascorbate-glutathione (AsA-GSH) metabolism in roots by upregulating the related enzymes activities and antioxidant contents under excess Se(IV) stress. These findings suggest that CHP14 is beneficial to improve plant growth and enhance Se(IV) resistance of Pakchoi, and can be exploited as potential inoculants for phytoremediation process in Se contaminated soil.

Bcwf regulates the white petal color in pak choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis]

Flower color is important in determining the ornamental value of Brassica species. However, our knowledge about the regulation of flower color in pak choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis] is limited. In this study, we investigated the molecular mechanism underlying white flower traits in pak choi by analyzing a genetic population with white and yellow flowers. Our genetic analysis revealed that the white trait is controlled by a single recessive gene called Bcwf. Through BSA-Seq and fine mapping, we identified a candidate gene, BraC02g039450.1, which is similar to Arabidopsis AtPES2 involved in carotenoid ester synthesis. Sequence analysis showed some mutations in the promoter region of Bcwf in white flowers. Tobacco transient assay confirmed that these mutations reduce the promoter's activity, leading to downregulation of Bcwf expression in white flowers. Furthermore, the silencing of Bcwf in pak choi resulted in lighter petal color and reduced carotenoid content. These findings provide new insights into the molecular regulation of white flower traits in pak choi and highlight the importance of Bcwf in petal coloring and carotenoid accumulation.

Construction of Greenhouse for Horticultural Cultivation with Soil Media and Hydroponics

Theneral background greenhouse technology is increasingly recognized as an efficient solution for sustainable agricultural practices, especially in regions with limited fertile land. Specific background In Kedungpeluk Village, where the majority of residents work as fish farmers, there are also unused and arid lands. These underutilized spaces provide an opportunity for greenhouse-based horticultural cultivation using soil media and hydroponic technology. Knowledge gap however, limited knowledge and infrastructure have prevented local farmers from effectively utilizing these lands for sustainable crop production. Aims this community engagement project aims to build and utilize a greenhouse system to cultivate horticultural plants, focusing on optimizing both soil and hydroponic methods. Results the project successfully transformed barren land into productive greenhouses that facilitate the growth of tomatoes, chilies, eggplants, pak choy, and water spinach. The greenhouse structure, designed with transparent roofing, enhances sunlight exposure, promoting optimal plant growth. Both soil and hydroponic methods showed potential for increasing crop yields, with soil cultivation being more flexible but requiring extra care for pests, while hydroponics provided faster results with minimal weed and pest interference. Novelty this project introduces a dual-method approach combining traditional soil cultivation with modern hydroponic technology in a controlled greenhouse environment. Implications the findings highlight that greenhouse farming is a viable, modern agricultural solution for rural communities, offering increased productivity and quality crops, while also addressing the challenges of limited fertile land and changing climate conditions. This project serves as a model for sustainable agriculture in similar settings. Highlights: Dual-method approach: Combining soil and hydroponic techniques in a greenhouse. Efficient land use: Transforming unused arid land for productive horticulture. Controlled environment: Optimizing plant growth through sunlight, temperature, and humidity control. Keywords: Greenhouse, Horticulture, Soil Media, Hydroponics, Sustainable Agriculture

Reclamation of co-pyrolyzed dredging sediment as soil cadmium and arsenic immobilization material: Immobilization efficiency, application safety, and underlying mechanisms

The safe management of toxic metal-polluted dredging sediment (DS) is imperative owing to its potential secondary hazards. Herein, the co-pyrolysis product (DS@BC) of polluted DS was creatively applied to immobilize soil Cd and As to achieve DS resource utilization, and the efficiency, safety, and mechanism were investigated. The results revealed that the DS@BC was more effective at reducing soil Cd bioavailability than the DS was (58.9–73.2% vs. 21.8–27.4%), except for the dilution effect, whereas the opposite phenomenon occurred for soil As (25.5–35.7% vs. 35.7–42.8%). The DS@BC immobilization efficiency was dose-dependent for both Cd and As. Soil labile Cd and As were transformed to more stable fractions after DS@BC immobilization. DS@BC immobilization inhibited the transfer of soil Cd and As to Brassica chinensis L. and did not cause excessive accumulation of other toxic metals in the plants. The appropriate addition of the DS@BC (8%) sufficiently alleviated the oxidative stress response of the plants and enhanced their growth. These findings indicate that the DS@BC was safe and effective for soil Cd and As immobilization. DS@BC immobilization decreased the diversity and richness of the rhizosphere soil bacterial community because of the dilution effect. The DS@BC immobilized soil Cd and As via direct adsorption, and indirect increasing soil pH, and regulating the abundance of specific beneficial bacteria (e.g., Bacillus). Therefore, the use of co-pyrolyzed DS as a soil Cd and As immobilization material is a promising resource utilization method for DS. Notably, to verify the long-term effects and safety of DS@BC immobilization, field trials should be conducted to explore the effectiveness and risk of harmful metal release from DS@BC immobilization under real-world conditions.

Passivation of metal sulfides by a marine bacterium for acid mine drainage control

Acid mine drainage originates from metal sulfides oxidation, which results in acidic metal-rich leachate. In this study, a novel and environmentally friendly approach was demonstrated to passivate pyrite and lead-zinc tailings, respectively. The key to this approach is to develop biofilms of the marine bacterium Qipengyuania flava S1. Biofilms can induce biomineralization, thereby isolating metal sulfides from air and water. The stability and biological toxicity of the bio-passivation layers were evaluated by leaching bio-passivated pyrite or tailings in initially acidic H2O2 solutions with shaking for 180 days and then cultivating Brassica chinensis and Allium cepa with the leachates. Our results showed that after passivation, the amount of iron released by pyrite decreased by at least 99.2 ± 0.2 (in wt%). For lead-zinc tailings after passivation, the released metal ions (Fe+Al+Pb+Zn) decreased by at least 52.0 ± 3.2 (in wt%). The bio-passivation layers also maintained the pH of the leachate in the range of 7.5–8.0. Before bio-passivation, compared with mineral water, the pyrite leachate significantly inhibited the growth of the two plants, and the tailings leachate significantly inhibited the growth of A. cepa, whereas the bio-passivated pyrite or tailings leachate did not show any inhibitory effect.

Management of soft rot in pakchoi (Brassica chinensis L.) using polyphenols from pineapple peel waste

Soft rot is one of the most susceptible soil-borne diseases of pakchoi (Brassica chinensis L.), severely hindering its industrial development. The research aimed to evaluate the application effect of polyphenols extracted from pineapple peel waste in improving the rhizosphere soil environment of pakchoi infected by soft rot and promoting plant health and growth. We found that using pineapple peel polyphenols (PPPs) at 4 mg/mL concentration (P1 treatment) significantly improved soil quality, reduced pakchoi disease incidence, and effectively promoted pakchoi growth. Compared with the control group (CK), the P1 treatment led to a marked 79.97 % reduction in diseased pakchoi leaves. Meanwhile, the rhizosphere soil structure showed reduced diseased genera and increased beneficial bacterial phyla, along with boosted soil enzyme activities. Furthermore, P1 treatment improved key growth parameters of pakchoi, including neck thickness (62.07 %), plant height (57.43 %), fresh weight (154.52 %), dry weight (121.88 %), maximum leaf length (47.28 %), width (30.98 %), and total chlorophyll content (50.11 %). The application of PPPs has shown significant effects in enhancing the ecological environment of the diseased soil and reducing the incidence of soft rot disease of pakchoi and has great potential in increasing the yield of pakchoi. This study offers an eco-friendly and sustainable approach for the valorization of PPPs and the management of soil-borne diseases, which holds profound implications for enhancing crop quality, soil health, and human well-being.

The Marine-Origin Exopolysaccharide-Producing Bacteria Micrococcus Antarcticus HZ Inhibits Pb Uptake in Pakchoi (Brassica chinensis L.) and Affects Rhizosphere Microbial Communities.

Exopolysaccharides (EPSs) produced by microorganisms play an important role in biotolerance and reducing heavy metal (HM) contamination by limiting the migration of HMs into plants. However, research on the application of EPS-producing marine bacteria for soil heavy metal remediation remains limited, particularly regarding their mechanisms of HM immobilization in soil and impact on plant growth. In this study, the EPS-producing marine bacterium Micrococcus antarcticus HZ was investigated for its ability to immobilize Pb and produce EPSs in soil filtrate. The effects on the growth quality and biomass of pakchoi (Brassica chinensis L.), as well as bacterial communities in inter-root soil contaminated with Pb, were also investigated. The results indicated that HZ could reduce the Pb concentration in the soil filtrate, achieving a removal rate of 43.25-63.5%. The EPS content and pH levels increased in the presence of Pb. Pot experiments showed that adding HZ significantly increased the biomass of pakchoi (9.45-14.69%), vitamin C (Vc) (9.69-12.92%), and soluble protein content (22.58-49.7%). HZ reduced the Pb content in the roots (17.52-47.48%) and leaves (edible tissues) (43.82-52.83%) of pakchoi. HZ increased soil enzyme activities (alkaline phosphatase, dehydrogenase, and urease), and the contents of ammonium nitrogen and nitrate nitrogen. Additionally, HZ also increased the relative abundance of beneficial bacteria (e.g., Proteobacteria, Cyanobacteria, and Chlorobacteria) in the inter-root soil, which have prophylactic and heavy-metal fixation functions. In summary, HZ reduces effective Pb content in edible tissues, roots, and inter-root soil by regulating inter-root soil microbial community structure, increasing soil pH, nitrogen content, and soil enzyme activity, and altering dominant phylum abundance.

Comprehensive effects of acetamiprid uptake and translocation from soil on pak choi and lettuce at the environmental level

Comprehensive effects of acetamiprid uptake and translocation from soil on pak choi and lettuce at the environmental level

Analysis of the correlation between Hashimoto's thyroiditis and food intolerance.

This study aims to explore the correlation between patients with Hashimoto's thyroiditis and food intolerance. A total of 172 subjects who visited Guangdong Provincial Hospital of Traditional Chinese Medicine between January 2020 and March 2023 were selected and tested for 90 food-specific IgG antibodies. The study group composed 85 individuals diagnosed with Hashimoto's thyroiditis, while the control group consisted of 87 healthy individuals. Data were analyzed to determine the correlation between Hashimoto's thyroiditis and food intolerance. Among the 85 patients with Hashimoto's thyroiditis, 97.65% exhibited food intolerance, with an average of 15.76 ± 10.61 types of food intolerances. The most common intolerances were to eggs (75.29%), bok choy (71.76%), and milk (65.88%), each exceeding a 60% intolerance rate. In the control group of 87 healthy individuals, the intolerance rate was 95.40%, with an average of 9.57 ± 8.90 types of food intolerances. The most prevalent intolerances in the control group were to bok choy (54.02%) and eggs (52.87%), each exceeding a 50% intolerance rate. The findings suggest that patients with Hashimoto's thyroiditis are more likely to develop food intolerance compared to the healthy population, which may indicate a correlation between Hashimoto's thyroiditis and food intolerance. Different dietary patterns may affect the activity of the thyroid axis and may even be the cause of autoimmune thyroid disease. The technique of detecting food intolerance IgG antibodies has the potential to be an important reference for dietary interventions in patients with Hashimoto's thyroiditis.

BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module promotes root development for improved salt tolerance in Bok choy

BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module promotes root development for improved salt tolerance in Bok choy

Seed coating with biocontrol bacteria encapsulated in sporopollenin exine capsules for the control of soil-borne plant diseases

Coating seeds with biocontrol agents represents an effective approach for managing soil-borne plant diseases. However, improving the viability of biocontrol microorganisms on the seed surface or in the rhizosphere remains a big challenge due to biotic and abiotic stresses. In this work, we developed a microbial seed coating strategy that uses sporopollenin exine capsules (SECs) as carriers for the encapsulation of the biofilm-like biocontrol bacteria. SECs was extracted from camellia bee pollen, and then characterized by Fourier Transform infrared spectroscopy (FTIR), elemental analysis and thermal gravity analysis (TG). The Paenibacillus polymyxa ZF129, a biocontrol bacterium, was introduced into SECs using the vacuum-incubation method and characterized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Notably, the ZF129 cell formed a biofilm-like structure inside the SECs, which enhanced their tolerance to acidic stress. As a proof of concept, we applied ZF129-loaded SECs to coat pak choi seeds using a straightforward plate-shaking technique. The coated seeds demonstrated a high control efficacy of up to 60.46 % against clubroot disease. Overall, this study sheds light on the application of SECs as promising carrier for the encapsulation of biofilm-like biocontrol bacteria, further augmenting the biocontrol functionality of microbial seed coating.

Synergistic effect of biochar and intercropping on lead phytoavailability in the rhizosphere of a vegetable-grass system

The effects of engineered steam exploded biochar on the phytoavailability of toxic elements in the shared- and nonshared-rhizosphere of vegetable-grass intercropping system have not been investigated yet. Therefore, we explored and elucidated the synergistic effect of pristine rape-straw biochar (BC), steam exploded BC (BCSE), KMnO4-modified BCSE (BCSEMn), and hydroxyapatite-modified BCSE (BCSEHA) on the solubility, fractionation and phytoavailability of lead (Pb) in a vegetable-grass intercropping system. In a rhizosphere box, Brassica chinensis L. (pakchoi; PC, as a vegetable) and Pennisetum polystachion L. (mission grass; MG, as a Pb hyperaccumulator), were grown in the biochar treated soil with (non-shared rhizosphere) or without (shared rhizosphere) root separation. Addition of BCSEMn and BCSEHA, particularly BCSEMn, significantly improved plant growth, photosynthetic pigment levels, and positively influenced the gas exchange attributes by suppressing oxidative stress and boosting antioxidant enzymes activities. Both biochars altered a proportion of Pb in the acid soluble to the immobile fraction and thus significantly decreased its leachability (TCLP-Pb) and bioavailability (CaCl2-extrcated Pb) by 32.7 %–33.9 % and 48.5 %–53.5 %, respectively, as compared to the control. Both biochars, particularly BCSEMn, reduced significantly the Pb content in shoots and roots of PC and MG with a significantly higher efficiency in the PC than in the MG; this was the case more in the shared than in the non-shared rhizosphere. These findings indicate the synergistic effect of BCSEMn and BCSEHA and intercropping for enhancing the grass phytostabilization capacity for Pb and reducing its uptake by edible plants in a vegetables-grass system, which could be used as a promising approach for the phytomanagement of Pb contaminated soils.

Selenium absorption, translocation and biotransformation in pak choi (Brassica chinensis L.) after foliar application of selenium nanoparticles

Diets consisting of selenium-deficient crops are associated with immune disorders and cardiomyopathy. Compared to the extensively used but highly toxic selenite (SeO32−), low-toxicity selenium nanoparticles (SeNPs) have emerged as a promising nanoplatform for Se biofortification in agriculture; however, the mechanisms underlying their transportation and biotransformation within crops remain elusive. In this study, SeNPs were successfully prepared using liquid-phase laser irradiation. We conducted a comparative study on the effects of foliar application of SeO32− and SeNPs on the growth of pak choi (Brassica chinensis L.), and investigated the absorption, translocation, and biotransformation mechanisms of Se in pak choi. The recommended dietary intake can be effectively achieved by applying SeNPs using leaf-spraying techniques. Our findings suggested that foliar application of SeNPs might be an efficient way to produce Se fortified crops, especially leafy vegetables, which are favorable for human health.

Non-thermal plasma enhances growth and salinity tolerance of bok choy (Brassica rapa subsp. chinensis) in hydroponic culture.

In this study, we aimed to examine the growth, physiological and biochemical status, and responses to salinity stress of bok choy (Brassica rapa subsp. chinensis) cultivated in a hydroponic system with a plasma-treated solution. Plasma gas generated using a cylindrical dielectric barrier discharge or air (control) was injected into Hoagland nutrient solution once a week for different durations (0, 5, and 10 min). After 4 weeks, the length of the shoots and roots, number of leaves, and dry weight of bok choy plants significantly increased in individuals grown with Hoagland solution treated with plasma gas for 10 min. An increase in dry weight of individual plants of approximately 80.5% was observed in plants in the plasma-treated group compared to those in a control group. The levels of chlorophyll, total soluble proteins, and nitrogen uptake, and transcription of genes related to salinity stress tolerance-WRKY2, HHP3, and ABI1- were also significantly elevated in bok choy grown with plasma treated Hoagland solution. Moreover, when exposed to 20 mM NaCl, plant length and leaf number were significantly increased, in the group grown with Hoagland solution treated with plasma gas for 10 min. Level of H2O2 was significantly elevated in the treated nutrient solutions. In plants grown with the treated nutrient solution, intracellular NO was highly detected in the cell division and elongation zone of roots. Our findings suggest that plasma treatment of nutrient solutions in hydroponic culture systems may improve the growth, physiological and biochemical status, and tolerance to salinity stress in plants, and a crucial role of H2O2 generated in the treated nutrient solutions may play in this improvement.