Branching Architecture Research Articles

Constructing branch architectures on fly ash surfaces to immobilize iron tetracarboxy phthalocyanine for synergistic degradation of dye contaminants

Utilization of fly ash (FA) solid wastes as the economical carrier materials to support the high active catalysts for environmental purification would contribute to the low carbon society, resource conservation and environmental improvement. For this purpose, in-depth understanding of the surface structure dependent fly ash carriers for the high efficiency immobilization of catalyst is essential. In this study, the highly active molecule catalysts of iron tetracarboxy phthalocyanine (TCFePc) complexes were successfully supported on FA for the first time. The FA particulates were successively activated to generate the dense branch crystal surfaces for immobilizing TCFePc complexes via a simple precipitation method. The TCFePc@FA composites can activate tert-butyl hydroperoxide (BuOOH) to produce hydroxyl and alkoxy radicals along with the generation of high valent iron-oxygen species to synergistically degrade high concentration organic dyes. Significantly, the TCFePc@FA-2% (2 wt% of catalyst) exhibited obviously higher catalytic efficiency than that of pure TCFePc. The immobilization mechanism and the catalytic processes of TCFePc@FA composites were discussed in detail. Our investigation provides an efficient and economical strategy for designing FA-based catalysts in the field of environmental catalysis.

QTL mapping of the narrow-branch “Pendula” phenotype in Norway spruce (Picea abies L. Karst.)

Pendula-phenotyped Norway spruce has a potential forestry interest for high-density plantations. This phenotype is believed to be caused by a dominant single mutation. Despite the availability of RAPD markers linked to the trait, the nature of the mutation is yet unknown. We performed a quantitative trait loci (QTL) mapping based on two different progenies of F1 crosses between pendula and normal crowned trees using NGS technologies. Approximately 25% of all gene bearing scaffolds of Picea abies genome assembly v1.0 were mapped to 12 linkage groups and a single QTL, positioned near the center of LG VI, was found in both crosses. The closest probe markers placed on the maps were positioned 0.82 cm and 0.48 cm away from the Pendula marker in two independent pendula-crowned × normal-crowned wild-type crosses, respectively. We have identified genes close to the QTL region with differential mutations on coding regions and discussed their potential role in changing branch architecture.

MDC-Net: a multi-directional constrained and prior assisted neural network for wood and leaf separation from terrestrial laser scanning

ABSTRACT Wood-leaf separation from terrestrial laser scanning (TLS) is a crucial prerequisite for quantifying many biophysical properties and understanding ecological functions. In this study, we propose a novel multi-directional collaborative convolutional neural network (MDC-Net) that takes the original 3D coordinates and useful features from prior knowledge (prior features) as input, and outputs the semantic labels of TLS point clouds. The MDC-Net contains two key units: (1) a multi-directional neighborhood construction (MDNC) unit to obtain more representative neighbors and enable directionally aware feature encoding in the subsequent local feature extraction, to mitigate occlusion effects; (2) a collaborative feature encoding (CFE) unit is introduced to incorporate useful features from prior knowledge into the network through a collaborative cross coding to enhance the discrimination for thin structures (e.g. small branches and leaf). The MDC-Net is evaluated on five plots from forests in Guangxi, China, with different branch architectures and leaf distributions. Experimental results showed that the MDC-Net achieved an OA of 0.973 and a mIoU of 0.821 and outperformed other related methods. We believe the MDC-Net would facilitate the usage of TLS in ecology studies for quantifying tree size and morphology and thus promote the development of relevant ecological applications.

Branch architecture in relation to canopy positions in three Mediterranean oaks

Branch architecture is a key determinant of plant performance owing to its role in a light interception by photosynthetic tissues. However, under stressed conditions, excess light may be harmful to the photosynthetic apparatus, and plants often present structural mechanisms to avoid photoinhibition. Three-dimensional models were constructed of the aerial parts in different locations within the crown of three co-occurring tree species (Quercus ilex, Q. suber and Q. faginea) growing in a Mediterranean environment. We hypothesized that the species with the shorter leaf life span would exhibit higher leaf display efficiency (silhouette to total leaf area, STAR), maximizing light interception and photosynthesis in the short term. In addition, more exposed positions within a canopy should develop more structural avoidance mechanisms to minimize excessive radiation. Significant differences were detected in architectural traits at both the intra- and interspecific level. Architectural traits promoting greater self-shading were more frequent in the species with longer leaf longevity and in the canopy locations experiencing higher temperatures at the times of maximum sunlight. However, these trends were in part counteracted by the changes in individual leaf area, which tended to be larger in the species with shorter leaf longevity and in the less exposed canopy locations. We conclude that the variation in architectural traits occurs mainly as a means to avoid the excessive self-shading of branches with the largest leaf size.

Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways.

Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our studies showed that the synthetic SL analog rac-GR24 and the biosynthetic inhibitor TIS108 affected stem length and diameter, aboveground weight, and chlorophyll content. The stem length of cherry rootstocks following TIS108 treatment reached a maximum value of 6.97cm, which was much higher than that following rac-GR24 treatments at 30 days after treatment. Stem paraffin section showed that SLs affected cell size. A total of 1936, 743, and 1656 differentially expressed genes (DEGs) were observed in stems treated with 10 μM rac-GR24, 0.1 μM rac-GR24, and 10 μM TIS108, respectively. RNA-seq results highlighted several DEGs, including CKX, LOG, YUCCA, AUX, and EXP, which play vital roles in stem growth and development. UPLC-3Q-MS analysis revealed that SL analogs and inhibitors affected the levels of several hormones in the stems. The endogenous GA3 content of stems increased significantly with 0.1 μM rac-GR24 or 10 μM TIS108 treatment, which is consistent with changes in the stem length following the same treatments. This study demonstrated that SLs affected stem growth of cherry rootstocks by changing other endogenous hormone levels. These results provide a solid theoretical basis for using SLs to modulate plant height and achieve sweet cherry dwarfing and high-density cultivation.

Meristem dormancy in Marchantia polymorpha is regulated by a liverwort-specific miRNA and a clade III SPL gene

The shape of modular organisms depends on the branching architecture, which in plants is determined by the fates of generative centers called meristems. The branches of the liverwort Marchantia polymorpha are derived from two adjacent meristems that develop at thallus apices. These meristems may be active and develop branches or may be dormant and do not form branches. The relative number and position of active and dormant meristems define the overall shape and form of the thallus. We show that the clade III SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor MpSPL1 is required for meristem dormancy. The activity of MpSPL1 is regulated by the liverwort-specific Mpo-MR13 miRNA, which, in turn, is regulated by PIF-mediated signaling. An unrelated PIF-regulated miRNA, MIR156, represses a different SPL gene (belonging to clade IV) that inhibits branching during the shade avoidance response in Arabidopsis thaliana. This suggests that a conserved light signaling mechanism modulates branching architecture in liverworts and angiosperms and therefore is likely operated in the last common ancestor. However, PIF-mediated signaling represses the expression of different miRNA genes with different SPL targets during dichotomous, apical branching in liverworts and during lateral, subapical branching in angiosperms. We speculate that the mechanism that acts downstream of light and regulates meristem dormancy evolved independently in liverworts and angiosperms.

Interspecies transfer of RAMOSA1 orthologs and promoter cis sequences impacts maize inflorescence architecture.

Grass inflorescences support floral structures that each bear a single grain, where variation in branch architecture directly impacts yield. The maize (Zea mays) RAMOSA1 (ZmRA1) transcription factor acts as a key regulator of inflorescence development by imposing branch meristem determinacy. Here, we show RA1 transcripts accumulate in boundary domains adjacent to spikelet meristems in sorghum (Sorghum bicolor, Sb) and green millet (Setaria viridis, Sv) inflorescences similar as in the developing maize tassel and ear. To evaluate the functional conservation of syntenic RA1 orthologs and promoter cis sequences in maize, sorghum, and setaria, we utilized interspecies gene transfer and assayed genetic complementation in a common inbred background by quantifying recovery of normal branching in highly ramified ra1-R mutants. A ZmRA1 transgene that includes endogenous upstream and downstream flanking sequences recovered normal tassel and ear branching in ra1-R. Interspecies expression of two transgene variants of the SbRA1 locus, modeled as the entire endogenous tandem duplication or just the nonframeshifted downstream copy, complemented ra1-R branching defects and induced unusual fasciation and branch patterns. The SvRA1 locus lacks conserved, upstream noncoding cis sequences found in maize and sorghum; interspecies expression of a SvRA1 transgene did not or only partially recovered normal inflorescence forms. Driving expression of the SvRA1 coding region by the ZmRA1 upstream region, however, recovered normal inflorescence morphology in ra1-R. These data leveraging interspecies gene transfer suggest that cis-encoded temporal regulation of RA1 expression is a key factor in modulating branch meristem determinacy that ultimately impacts grass inflorescence architecture.

Variant anatomy of the colonic and aberrant branches of the superior mesenteric artery

Background. The rapid development of abdominal, endovascular surgery and transplantology necessitate a detailed study of the structural features of the abdominal cavity vessels.
 Aim. To study the variant anatomy of the colonic branches of the superior mesenteric artery in men and women.
 Material and methods. The study was conducted based on the analysis of the results of multislice spiral computed tomography, a total of 2300 computed tomograms of adults aged 25 to 75 years (913 males and 1387 females) were studied. To assess the statistical significance of the aorta branches architectonics variants frequency dependence on gender, the method of analyzing four-field tables (Pearson's chi-square test) was used. The correlation strength was assessed using the normalized value of the Pearson coefficient.
 Results. It has been established that the branches of the superior mesenteric artery, which vascularize the large intestine, have a wide range of variant anatomy. According to their number and the presence of aberrant branches, these variants can be classified into mono-, bi-, tri-, quadri- and penta-arterial. The most common variant of the architectonics of these branches of the superior mesenteric artery is biarterial. Mono- and pentaarterial branching variants of the colonic branches of the superior mesenteric artery are extremely rare in both sexes. The iliocolic and middle colic arteries are permanent branches of the superior mesenteric artery, and the right colic artery may be absent (in 7% of cases in males and 19% in females). Among the aberrant branches of the superior mesenteric artery, we noted the arteries involved in the blood supply to the liver: the right accessory, right replacement, and common hepatic arteries. In men in 25% of cases, in women in 16% of cases, a deviation from the superior mesenteric artery of the aberrant hepatic branch was noted.
 Conclusion. The architecture of the colonic branches of the superior mesenteric artery is characterized by a wide range of variant anatomy and a high incidence of aberrant hepatic arteries.

A Reconfigurable Neural Architecture for Edge–Cloud Collaborative Real-Time Object Detection

Although recent advances in deep neural networks (DNNs) have enabled remarkable performance on various computer vision tasks, it is challenging for edge devices to perform real-time inference of complex DNN models due to their stringent resource constraints. To enhance the inference throughput, recent studies have proposed collaborative intelligence (CI), which splits DNN computation into edge and cloud platforms, mostly for simple tasks, such as image classification. However, for general DNN-based object detectors with a branching architecture, CI is highly restricted because of a significant feature transmission overhead. To resolve this issue, in this study, we propose a reconfigurable DNN architecture for real-time object detection that can configure the optimal split point according to the edge–cloud CI environment. The proposed architecture allows the DNN model to be splittable by a feature reconstruction network and asymmetric scaling. Based on the splittable architecture, we integrate independent splittable models for each split point into a single-weight reconfigurable model that enables multipath inference by switchable quantization and distribution matching. Finally, we introduce an adaptive application procedure of the reconfigurable model for efficient CI, which includes asymmetric scale configuration and split point selection. The performance evaluation using YOLOv5 as the baseline showed that the proposed architecture achieved 30 frames/s ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$2.6\times $ </tex-math></inline-formula> and <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$1.6\times $ </tex-math></inline-formula> higher than edge-only and cloud-only inference, respectively), on the NVIDIA Jetson TX2 platform in a WiFi environment.

Endoscopic Features of Gastric Epithelial Neoplasm of Fundic Gland Mucosa Lineage

The endoscopic features of gastric epithelial neoplasms of fundic gland mucosa lineage (GEN-FGML) have not been well investigated. We aimed to clarify the endoscopic features of GEN-FGML and differences between gastric adenocarcinoma of the fundic gland type (GA-FG) and fundic gland mucosa type (GA-FGM). A total of 62 GEN-FGML lesions, including 52 GA-FG and 10 GA-FGM, were retrospectively analyzed using endoscopic and clinicopathological findings to provide information of diagnostic value using white light imaging (WLI) and magnifying endoscopy with narrow-band imaging (M-NBI). GA-FG frequently presented with a whitish, submucosal tumor (SMT) shape with dilated vessels with branching architecture and background mucosa without atrophic change in WLI, an indistinct demarcation line (DL), dilatation of the crypt opening and intervening part (IP), and microvessels without distinct irregularity in M-NBI. GA-FGM frequently presented as a reddish, elevated lesion in WLI, with a distinct DL, dilatation of the IP, and an irregular microvascular pattern in M-NBI. As for an M-NBI diagnosis, five GA-FGM lesions met the diagnostic criteria for cancer, whereas none of the GA-FG lesions met the same criteria. We highlight the endoscopic features of GEN-FGML, and the differentiation between GA-FG and GA-FGM might be possible by combination of lesion color and morphology in WLI and M-NBI diagnoses.

Genetic dissection of branch architecture in oilseed rape (Brassica napus L.) germplasm.

Branch architecture is an important factor influencing rapeseed planting density, mechanized harvest, and yield. However, its related genes and regulatory mechanisms remain largely unknown. In this study, branch angle (BA) and branch dispersion degree (BD) were used to evaluate branch architecture. Branch angle exhibited a dynamic change from an increase in the early stage to a gradual decrease until reaching a stable state. Cytological analysis showed that BA variation was mainly due to xylem size differences in the vascular bundle of the branch junction. The phenotypic analysis of 327 natural accessions revealed that BA in six environments ranged from 24.3° to 67.9°, and that BD in three environments varied from 4.20cm to 21.4cm, respectively. A total of 115 significant loci were detected through association mapping in three models (MLM, mrMLM, and FarmCPU), which explained 0.53%-19.4% of the phenotypic variations. Of them, 10 loci were repeatedly detected in different environments and models, one of which qBAD.A03-2 was verified as a stable QTL using a secondary segregation population. Totally, 1066 differentially expressed genes (DEGs) were identified between branch adaxial- and abaxial- sides from four extremely large or small BA/BD accessions through RNA sequencing. These DEGs were significantly enriched in the pathways related to auxin biosynthesis and transport as well as cell extension such as indole alkaloid biosynthesis, other glycan degradation, and fatty acid elongation. Four known candidate genes BnaA02g16500D (PIN1), BnaA03g10430D (PIN2), BnaC03g06250D (LAZY1), and BnaC06g20640D (ARF17) were identified by both GWAS and RNA-seq, all of which were involved in regulating the asymmetric distribution of auxins. Our identified association loci and candidate genes provide a theoretical basis for further study of gene cloning and genetic improvement of branch architecture.

Considerate motion imagination classification method using deep learning.

In order to improve the classification accuracy of motion imagination, a considerate motion imagination classification method using deep learning is proposed. Specifically, based on a graph structure suitable for electroencephalography as input, the proposed model can accurately represent the distribution of electroencephalography electrodes in non-Euclidean space and fully consider the spatial correlation between electrodes. In addition, the spatial-spectral-temporal multi-dimensional feature information was extracted from the spatial-temporal graph representation and spatial-spectral graph representation transformed from the original electroencephalography signal using the dual branch architecture. Finally, the attention mechanism and global feature aggregation module were designed and combined with graph convolution to adaptively capture the dynamic correlation intensity and effective feature of electroencephalography signals in various dimensions. A series of contrast experiments and ablation experiments on several different public brain-computer interface datasets demonstrated that the excellence of proposed method. It is worth mentioning that, the proposed model is a general framework for the classification of electroencephalography signals, which is suitable for emotion recognition, sleep staging and other fields based on electroencephalography research. Moreover, the model has the potential to be applied in the medical field of motion imagination rehabilitation in real life.

The branching architecture of artemisia ordosica and its resistance to wind erosion

Different branching architectures reflect the adaptation strategies of different plants and affect their resistance to wind erosion. This study presents field-based observations that demonstrate the relationship between the branching architecture of Artemisia ordosica and its resistance to wind erosion. This species is the dominant plant species in the semi-fixed and fixed dunes of the Mu Us Sandy land. The overall bifurcation ratio (OBR) of semi-fixed sandy land is higher than the fixed sandy land 0.27; Similarly, the total stepwise bifurcation ratio (SBR) is higher than the fixed sandy land about 0.74; The length of first levels of total branches is also higher than 8.07. The aerodynamic roughness was greater than the A. ordosica community in the fixed and semi-fixed sandy land than in the bare sandy land. The airflow fields in the cross-wind direction were strongly affected by the windward shape of the plants, which became gradually narrower from the base to the top, while in the leeward direction, the wind speed at different heights behind the plant returned to the incoming airflow velocity. The result confirms that the influence of the windward shape of the plant on the surrounding airflow field is much larger than the influence of plant thickness, porosity or other factors.

Lipophilic PAMAM Dendrimer: Conceptualization of Targeted Cosmetics and Drug Delivery

Abstract: The structure, properties, synthesis, negligible toxicity, and surface modification of PAMAM (polyamidoamine dendrimers) are all discussed in this review. The properties of supramolecular PAMAM dendrimers in nanopolymer science have shown great progress in delivering medicines. A divergent strategy was used to construct a Generation four (G4.0) PAMAM dendrimer with an ethylenediaminetetraacetic acid core and repeating units of acrylic acid and ethylenediamine. PAMAM dendrimers, have an aminodoamine repeat branching architecture that starts with an ethylene diamine initiator core. A generation [G] is a set of branching steps that follow each other. Drug molecules can be transferred either as covalently bonded to the functional groups on the dendrimer surface or by forming non-covalent complexes with dendrimers. Full generation PAMAM dendrimers are terminated with amine surface [G0, G1, G2, G3, G4], whereas half-generation dendrimers are terminated with carboxylate [G1.5, G2.5, etc]. PAMAM dendrimers appear to have negligible toxicity and immunogenicity, as well as favorable biodistribution-: according to the current study they can improve drug solubility, prevent drug degradation, increase circulation time, and potentially target drugs. According to the characterization study, they exhibit strong lipophilic qualities, allowing them to easily pass the blood-brain barrier. Due to cheaper polydispersity index of dendrimers, they possess greater stability and the void spaces of dendrimers are accessible for drug loading. The existence of a duplet functional group on the dendrimers enables appending vectors, ligands and devices for targed the drug delivery in the body.

Liriodendron chinense LcMAX1 regulates primary root growth and shoot branching in Arabidopsis thaliana

Strigolactones (SLs) play prominent roles in regulating shoot branching and root architecture in model plants. However, their roles in non-model (particularly woody) plants remain unclear. Liriodendron chinense is a timber tree species widely planted in southern China. The outturn percentage and wood quality of L. chinense are greatly affected by the branching characteristics of its shoot, and the rooting ability of the cuttings is key for its vegetative propagation. Here, we isolated and analyzed the function of the MORE AXILLARY GROWTH 1 (LcMAX1) gene, which is involved in L. chinense SL biosynthesis. RT-qPCR showed that LcMAX1 was highly expressed in the roots and axillary buds. LcMAX1 was located in the endoplasmic reticulum (ER) and nucleus. LcMAX1 ectopic expression promoted primary root growth, whereas there were no phenotypic differences in shoot branching between transgenic and wild-type (WT) A. thaliana plants. LcMAX1 overexpression in the max1 mutant restored them to the WT A. thaliana phenotypes. Additionally, AtPIN1, AtPIN2, and AtBRC1 expressions were significantly upregulated in transgenic A. thaliana and the max1 mutant. It was therefore speculated that LcMAX1 promotes primary root growth by regulating expression of auxin transport-related genes in A. thaliana, and LcMAX1 inhibits shoot branching by upregulating expression of AtBRC1 in the max1 mutant. Altogether, these results demonstrated that the root development and shoot branching functions of LcMAX1 were similar to those of AtMAX1. Our findings provide a foundation for obtaining further insights into root and branch development in L. chinense.

Effect of Different Cytokinins on Shoot Outgrowth and Bioactive Compounds Profile of Lemograss Essential Oil

Lemongrass (Cymbopogon citratus) essential oil (EO) is a major source of bioactive compounds (BC) with anticancer activity such as α-citral, limonene, geraniol, geranyl acetate, and β-caryophyllene. Comparative studies about cytokinin effects on BC profiles in lemongrass are missing. Here, we evaluated four cytokinins (2iP, tZ, BAP, and KIN) in two different osmotic media, MS-N (3% sucrose, 3 g L−1 Gelrite™) and MS-S (5% sucrose, 5 g L−1 Gelrite™). It results in a higher multiplication rate in BAP containing medium compared to tZ, KIN, and 2iP (p ≤ 0.05). While shoots grown on MS-N/BAP, tZ, and KIN exhibited a highly branching morphology, MS-N/2iP produced a less branching architecture. BC profile analysis of established plants in pots revealed that their maxima production depends on the in vitro shoot growth conditions: i.e., highest content (80%) of α-citral in plants that were cultured in MS-S/BAP (p ≤ 0.05), limonene (41%) in MS-N/2iP, or geranyl acetate (25.79%) in MS-S/2iP. These results indicate that it is possible to increase or address the production of BC in lemongrass by manipulating the cytokinin type and osmotic pressure in culture media. The culture protocol described here is currently successfully applied for somatic embryogenesis induction and genetic transformation in lemongrass.

Role of a ZF-HD Transcription Factor in miR157-Mediated Feed-Forward Regulatory Module That Determines Plant Architecture in Arabidopsis.

In plants, vegetative and reproductive development are associated with agronomically important traits that contribute to grain yield and biomass. Zinc finger homeodomain (ZF-HD) transcription factors (TFs) constitute a relatively small gene family that has been studied in several model plants, including Arabidopsis thaliana L. and Oryza sativa L. The ZF-HD family members play important roles in plant growth and development, but their contribution to the regulation of plant architecture remains largely unknown due to their functional redundancy. To understand the gene regulatory network controlled by ZF-HD TFs, we analyzed multiple loss-of-function mutants of ZF-HD TFs in Arabidopsis that exhibited morphological abnormalities in branching and flowering architecture. We found that ZF-HD TFs, especially HB34, negatively regulate the expression of miR157 and positively regulate SQUAMOSA PROMOTER BINDING–LIKE 10 (SPL10), a target of miR157. Genome-wide chromatin immunoprecipitation sequencing (ChIP-Seq) analysis revealed that miR157D and SPL10 are direct targets of HB34, creating a feed-forward loop that constitutes a robust miRNA regulatory module. Network motif analysis contains overrepresented coherent type IV feedforward motifs in the amiR zf-HD and hbq mutant background. This finding indicates that miRNA-mediated ZF-HD feedforward modules modify branching and inflorescence architecture in Arabidopsis. Taken together, these findings reveal a guiding role of ZF-HD TFs in the regulatory network module and demonstrate its role in plant architecture in Arabidopsis.

Tree height-growth trajectory estimation using uni-temporal UAV laser scanning data and deep learning

Abstract Information on tree height-growth dynamics is essential for optimizing forest management and wood procurement. Although methods to derive information on height-growth information from multi-temporal laser scanning data already exist, there is no method to derive such information from data acquired at a single point in time. Drone laser scanning data (unmanned aerial vehicles, UAV-LS) allows for the efficient collection of very dense point clouds, creating new opportunities to measure tree and branch architecture. In this study, we examine if it is possible to measure the vertical positions of branch whorls, which correspond to nodes, and thus can in turn be used to trace the height growth of individual trees. We propose a method to measure the vertical positions of whorls based on a single-acquisition of UAV-LS data coupled with deep-learning techniques. First, single-tree point clouds were converted into 2D image projections, and a YOLOv5 (you-only-look-once) convolutional neural network was trained to detect whorls based on a sample of manually annotated images. Second, the trained whorl detector was applied to a set of 39 trees that were destructively sampled after the UAV-LS data acquisition. The detected whorls were then used to estimate tree-, plot- and stand-level height-growth trajectories. The results indicated that 70 per cent (i.e. precision) of the measured whorls were correctly detected and that 63 per cent (i.e. recall) of the detected whorls were true whorls. These results translated into an overall root-mean-squared error and Bias of 8 and −5 cm for the estimated mean annual height increment. The method’s performance was consistent throughout the height of the trees and independent of tree size. As a use case, we demonstrate the possibility of developing a height-age curve, such as those that could be used for forecasting site productivity. Overall, this study provides proof of concept for new methods to analyse dense aerial point clouds based on image-based deep-learning techniques and demonstrates the potential for deriving useful analytics for forest management purposes at operationally-relevant spatial-scales.

Zebrafish Erc1b mediates motor innervation and organization of craniofacial muscles in control of jaw movement.

Movement of the lower jaw, a common behavior observed among vertebrates, is required for eating and processing food. This movement is controlled by signals sent from the trigeminal motor nerve through neuromuscular junctions (NMJs) to the masticatory muscles. Dysfunctional jaw movements contribute to craniomandibular disorders, yet the pathophysiology of these disorders is not well understood, as limited studies have been conducted on the molecular mechanisms of jaw movement. Using erc1b/kimm533 genetic loss of function mutant, we evaluated lower jaw muscle organization and innervation by the cranial motor nerves in developing zebrafish. Using time-lapse confocal imaging of the erc1b mutant in a transgenic fluorescent reporter line, we found delayed trigeminal nerve growth and disrupted nerve branching architecture during muscle innervation. By automated 3D image analysis of NMJ distribution, we identified an increased number of small, disorganized NMJ clusters in erc1b mutant larvae compared to WT siblings. Using genetic replacement experiments, we determined the Rab GTPase binding domain of Erc1b is required for cranial motor nerve branching, but not NMJ organization or muscle attachment. We identified Erc1b/ERC1 as a novel component of a genetic pathway contributing to muscle organization, trigeminal nerve outgrowth, and NMJ spatial distribution during development that is required for jaw movement.

The Impact of Visual Defects and Neighboring Trees on Wind-Related Tree Failures

Urban trees are often more sun- and wind-exposed than their forest-grown counterparts. These environmental differences can impact how many species grow–impacting trunk taper, crown spread, branch architecture, and other aspects of tree form. Given these differences, windthrow models derived from traditional forest production data sources may not be appropriate for urban forest management. Additionally, visual abnormalities historically labeled as “defects” in timber production, may not have a significant impact on tree failure potential. In this study, we look at urban tree failures associated with Hurricane Irma in Tampa, Florida, USA. We used spatial analysis to determine if patterns of failure existed among our inventoried trees. We also looked at risk assessment data to determine which visual defects were the most common and the most likely to be associated with branch or whole-tree failure. Results indicate that there was no spatial pattern associated with the observed tree failures–trees failed or withstood the storm as individuals. While some defects like decay and dead wood were associated with increased tree failure, other defects such as weak branch unions and poor branch architecture were less problematic.