The present experiment is to analyze the effect of blended biodiesel of chicken fat CF10 (10% chicken fat and 90% diesel), waste cooking oil WC10 (10% waste cooking oil and 90% diesel), and combined chicken fat with waste cooking oil CF&WC10 (5% chicken fat + 5% waste cooking oil and 90% diesel) on the performance of diesel engine. The experiment is conducted at a constant compression ratio of 17, mixing 50 ppm alumina alfa (Al2O3-α) catalyst nanoparticles in each blend. The performance of biodiesel is analyzed at 25%, 50%, 75%, and 100% load conditions and is compared with pure diesel. The results show that the brake torque (BT) and brake power of CF&WC10 were found to be 22.48 Nm and 3.58 kW at full load conditions. However, the indicated thermal efficiency and BTE of CF&WC10 are at full loading conditions of 52.45% and 26.78% respectively. Because the supply of A/F ratio is 18.81, at full load condition and also lowering the brake specific fuel consumption up to 0.32 kg/kWh. Therefore, optimization of both performance and exhaust emission of an engine depends on BTE and NOx as well as HC emissions. Hence, from the emissions reduction point of view, CF&WC10 has lower emissions while achieving brake thermal efficiency similar to diesel fuel. The novelty of research shows CF&WC10 leads to enhanced performance of diesel engine compared to pure diesel fuel.