BP1 Cells Research Articles

Expression of Forssman antigen of avian lymphoblastoid cell lines transformed by Marek's disease virus or avian leukosis virus.

The expression of Forssman-type heterophile antigen on Marek's disease (MD) virus (MDV)-transformed cell lines, MDCC-MSBI 1, -HP1, -RP1 and -BP1, and avian leukosis virus (ALV)-transformed cell lines, LSCC-1104B1 and -1104X5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. Forssman antigen was detected on a high percentage of the cells in two ALV-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. Of the MDV-transformed cell lines tested only the RP1 and BP1 cell lines, derived from transplantable MD tumours, expressed Forssman antigen, while the MSB1 and HP1 cell lines, derived from MD lymphomas, did not. Forssman antigen appears to be unrelated to MD tumour-associated surface antigen (MATSA).

Production of cytotoxic antibody to a benz(a)pyrene-induced sarcoma in mice receiving xenogeneic antitumor immune RNA

Groups of normal C3Hf mice were injected subcutaneously and intraperitoneally over a 2-week period with various xenogeneic immune RNA (I-RNA) preparations. I-RNAs were extracted from the lymphoid organs of guinea pigs following immunization with normal C3Hf tissue cells, or tumor cells from a benz(a)pyrene-induced sarcoma (BP-1), a methylcholanthrene-induced sarcoma MC-1, or a spontaneous mammary carcinoma (SMT). In sera from mice injected with anti-BP-1 I-RNA, antibodies were detected which were specifically cytotoxic to BP-1 target cells, in vitro, but not to MC-1 target cells. Absorption with BP-1 sarcoma cells removed this cytotoxic activity while absorption with syngeneic spleen cells, or MC-1 sarcoma cells did not. Sera from mice receiving any of the other I-RNAs were not cytotoxic for either BP-1 or MC-1 target cells. Treatment of mice with BP-1 I-RNA may have induced the production of tumor-specific cytotoxic antibody, in vivo.