Introduction: In vitro and in vivo approaches are currently being used for guided cell repopulation of both biological and synthetic scaffolds. Till now, researchers have used different strategies to decellularize biological samples such as the use of detergents like SDS, 1× Triton-X and EDTA solutions. However, their widespread clinical acceptance is very limited. In the present study, different tissueengineered biological conduits and caffolds made from bovine carotid artery (BCA) have been developed using cultured endothelial cells from sheep external jugular vein endothelial cells (SEJVECs). Materials and methods: Conduit preparation was achieved as follows: (a) 0.2% glutaraldehyde fixation and (b) scaffold preparation by decellularization method using 75% alcohol–0.25% trypsin treatment at two different time intervals. The percentage of decellularization was calculated for all the prepared conduits (n = 3), and it was observed that longer exposure to alcohol and trypsin leads to better decellularization. Endothelial cells were seeded on the conduits prepared by the above methods. Results: About 0.2% glutaraldehyde-fixed conduits showed better attachment of endothelial cells, as compared with ethanol-trypsin–treated decellularized conduits. These results demonstrate the use of BCA as a source of biological conduits in the creation of a new small-diameter tissue-engineered vascular graft. Conclusion:This study shows that it is possible to prepare conduit and scaffold (glutaraldehyde- treated and decellularized) for seeding endothelial cells on their luminal surface, which which can be used for vascular grafting.