According to the requirements of boundary layer transition detection measurement flight test, the study on the detection technique of infrared thermal image in real flight conditions was carried out. In this paper, the basic principle of infrared measurement technique was introduced firstly. Through the analysis of the main factors, such as emissivity, radiation incidence angle and temperature difference between the measured object and environment, the test means were determined, which includes surface treatment, infrared light path adjustment, solar radiation and internal heating of tested parts. Then the whole flight test method of boundary layer transition measurement was designed, including selecting test object and test instrument, determining test procedure and image processing procedure, and giving transition location criterion. Finally, the validity of this flight test method was demonstrated through testing results, the research of which would be helpful and meaningful in boundary layer transition measurement.