On Haruku Island, Maluku Province, there is Adat Sasi where fishing activities and their use are regulated based on the culture of the local community from hereditary inherited from their ancestors. Adat Sasi is a prohibition against taking natural products in the form of agriculture and marine fisheries before the specified time so that the environment is preserved. On the other hand, Law Number 23 of 2014 concerning Regional Government regulates the boundaries of provincial authority in the sea area. The question is whether the fishing zone which is bound by Adat Sasi follows the National regulation? In this paper, Marine Fisheries Zoning Based on Adat Sasi Indigenous Local Wisdom is technically reviewed based on the principle of hydrography, which is one of the scientific fields related to positioning activities at sea. The methodology used is to search for information on the boundaries of the fishing zone that is tied to Adat Sasi, and then quantify it numerically to be compared with the Sea Boundary Map of Maluku Province. The results of the study indicate that the fishing zone boundary which is related to Adat Sasi is approximately 5.04 km from the coastline. While the provincial authority in the sea area is as far as 22.2 km. Thus, the Provincial Government has the opportunity to expand the fishing zone based on Adat Sasi or have other alternatives to determine zoning for coastal areas by considering Adat Sasi in the framework of sustainable nature conservation.