Bridges are generally affected by thermal loads which include the daily cycle, seasonal cycle and annual cycle. Thermal loads mode and thermal effects on bridges, especially for concrete girders, are quite essential but complicated. To investigate the temperature field and thermal stress in the thickness direction of a concrete box girder, the temperature field of a prestressed concrete continuous box girder bridge is monitored, and the temperature distribution in the thickness direction of the concrete box girder is analyzed. Finite element simulation, utilizing air elements specifically designed for concrete box girders, is employed to analyze the temperature field and thermal stress profiles along the thickness of the slab. The findings indicate a variation in temperature along the thickness of the concrete box girder slab. The most significant temperature differential, reaching up to 10.7 °C, is observed along the thickness of the top slab, followed by the bottom plate, with the web exhibiting relatively smaller differentials. Temperature in the full thickness range has a significant impact on the top plate, while the web plate and bottom plate are greatly influenced by temperature ranging from the outer surface to the center of the plate thickness. The temperature difference between the center of the plate thickness and the inner surface is approximately 0. The variation in temperature due to the variation in thickness direction is a temporal factor, wherein the outer layer of the roof is primarily compressed, while the inner layer is subjected to tension. The external surface of the web is mainly compressed. The stress exerted by the internal surface temperature is minimal. The internal and external surface effects of the floor are similar, and as time passes, tensile and compressive stresses appear.